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Protein

Chaperonin 60 subunit alpha 1, chloroplastic

Gene

CPN60A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds RuBisCO small and large subunits and is implicated in the assembly of the enzyme oligomer. Involved in protein assisted folding. Required for proper chloroplast development.2 Publications

Miscellaneous

Assisted protein folding requires ATP hydrolysis, but not K+ ions.

GO - Molecular functioni

GO - Biological processi

  • 'de novo' protein folding Source: GO_Central
  • chaperone-mediated protein folding Source: GO_Central
  • chloroplast organization Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • mitochondrion organization Source: GO_Central
  • protein folding Source: TAIR
  • protein refolding Source: InterPro

Keywordsi

Molecular functionChaperone
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperonin 60 subunit alpha 1, chloroplastic
Short name:
CPN-60 alpha 1
Alternative name(s):
Protein SCHLEPPERLESS
RuBisCO large subunit-binding protein subunit alpha 1
Gene namesi
Name:CPN60A1
Synonyms:Cpn60-A(2), SLP
Ordered Locus Names:At2g28000
ORF Names:T1E2.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G28000
TAIRilocus:2057841 AT2G28000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryos are albino, can germinate but are unable to produce viable seedlings.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi335D → A: Retarded growth and pale-green leaves. 1 Publication1
Mutagenesisi342A → V in arc2; fewer, but larger chloroplasts. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46ChloroplastBy similarityAdd BLAST46
ChainiPRO_000000501647 – 586Chaperonin 60 subunit alpha 1, chloroplasticAdd BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21238
PRIDEiP21238

2D gel databases

SWISS-2DPAGEiP21238

PTM databases

iPTMnetiP21238
SwissPalmiP21238

Expressioni

Tissue specificityi

Expressed in leaves, stems, siliques and flowers.1 Publication

Inductioni

Up-regulated by light.1 Publication

Gene expression databases

ExpressionAtlasiP21238 baseline and differential
GenevisibleiP21238 AT

Interactioni

Subunit structurei

Part of the Cpn60 complex composed of 7 alpha and 7 beta subunits. This complex shows ATPase activity. The Cpn60 complex interacts with the Cpn10 complex.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2694, 6 interactors
IntActiP21238, 2 interactors
STRINGi3702.AT2G28000.1

Structurei

3D structure databases

ProteinModelPortaliP21238
SMRiP21238
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chaperonin (HSP60) family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0356 Eukaryota
COG0459 LUCA
HOGENOMiHOG000076290
InParanoidiP21238
OMAiTEGMMVE
OrthoDBiEOG093606F2
PhylomeDBiP21238

Family and domain databases

CDDicd03344 GroEL, 1 hit
Gene3Di1.10.560.10, 2 hits
3.30.260.10, 2 hits
3.50.7.10, 1 hit
HAMAPiMF_00600 CH60, 1 hit
InterProiView protein in InterPro
IPR018370 Chaperonin_Cpn60_CS
IPR001844 Chaprnin_Cpn60
IPR002423 Cpn60/TCP-1
IPR027409 GroEL-like_apical_dom_sf
IPR027413 GROEL-like_equatorial_sf
IPR027410 TCP-1-like_intermed_sf
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PRINTSiPR00298 CHAPERONIN60
SUPFAMiSSF48592 SSF48592, 2 hits
SSF52029 SSF52029, 1 hit
SSF54849 SSF54849, 2 hits
TIGRFAMsiTIGR02348 GroEL, 1 hit
PROSITEiView protein in PROSITE
PS00296 CHAPERONINS_CPN60, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21238-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASANALSSA SVLCSSRQSK LGGGNQQQGQ RVSYNKRTIR RFSVRANVKE
60 70 80 90 100
IAFDQHSRAA LQAGIDKLAD CVGLTLGPRG RNVVLDEFGS PKVVNDGVTI
110 120 130 140 150
ARAIELPNAM ENAGAALIRE VASKTNDSAG DGTTTASILA REIIKHGLLS
160 170 180 190 200
VTSGANPVSL KRGIDKTVQG LIEELQKKAR PVKGRDDIRA VASISAGNDD
210 220 230 240 250
LIGSMIADAI DKVGPDGVLS IESSSSFETT VEVEEGMEID RGYISPQFVT
260 270 280 290 300
NPEKLLAEFE NARVLITDQK ITAIKDIIPI LEKTTQLRAP LLIIAEDVTG
310 320 330 340 350
EALATLVVNK LRGVLNVVAV KAPGFGERRK AMLQDIAILT GAEYLAMDMS
360 370 380 390 400
LLVENATIDQ LGIARKVTIS KDSTTLIADA ASKDELQARI AQLKKELFET
410 420 430 440 450
DSVYDSEKLA ERIAKLSGGV AVIKVGAATE TELEDRKLRI EDAKNATFAA
460 470 480 490 500
IEEGIVPGGG AALVHLSTVI PAIKETFEDA DERLGADIVQ KALLSPAALI
510 520 530 540 550
AQNAGVEGEV VVEKIMFSDW ENGYNAMTDT YENLFEAGVI DPAKVTRCAL
560 570 580
QNAASVAGMV LTTQAIVVDK PKPKAPAAAA PEGLMV
Length:586
Mass (Da):62,072
Last modified:November 1, 1997 - v2
Checksum:i71BCBDC81C7905B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184G → D in AAM63618 (Ref. 5) Curated1
Sequence conflicti186D → V in AAA32724 (PubMed:1979547).Curated1
Sequence conflicti188I → Y in AAA32724 (PubMed:1979547).Curated1
Sequence conflicti569D → G in BAD95121 (Ref. 7) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49357 Genomic DNA Translation: AAA92061.1
AC006929 Genomic DNA Translation: AAD21502.1
CP002685 Genomic DNA Translation: AEC08068.1
BT002441 mRNA Translation: AAO00801.1
BT008784 mRNA Translation: AAP68223.1
AY086555 mRNA Translation: AAM63618.1
M35597 mRNA Translation: AAA32724.1
AK222126 mRNA Translation: BAD95121.1
PIRiS71235
RefSeqiNP_180367.1, NM_128359.5
UniGeneiAt.24154
At.67332

Genome annotation databases

EnsemblPlantsiAT2G28000.1; AT2G28000.1; AT2G28000
GeneIDi817344
GrameneiAT2G28000.1; AT2G28000.1; AT2G28000
KEGGiath:AT2G28000

Similar proteinsi

Entry informationi

Entry nameiCPNA1_ARATH
AccessioniPrimary (citable) accession number: P21238
Secondary accession number(s): Q42554, Q56WB8, Q8L5U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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