P21237 (BDNF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Brain-derived neurotrophic factor
Short name=BDNF

Gene names

Name:

Bdnf

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.
Subunit structure	Monomers and homodimers. Binds to NTRK2/TRKB. Ref.3
Subcellular location	Secreted.
Post-translational modification	Converted into mature BDNF by plasmin (PLG).
Sequence similarities	Belongs to the NGF-beta family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Inferred from mutant phenotype PubMed 15014133. Source: MGI axon target recognition Inferred from mutant phenotype PubMed 15464571. Source: MGI dendrite development Inferred from mutant phenotype PubMed 12890780. Source: MGI fear response Inferred from mutant phenotype PubMed 15249684. Source: MGI feeding behavior Inferred from mutant phenotype PubMed 12796784. Source: MGI glutamate secretion Inferred from physical interaction PubMed 16819522. Source: MGI inner ear development Inferred from mutant phenotype PubMed 7605630. Source: MGI learning or memory Inferred from mutant phenotype PubMed 15249684. Source: MGI mechanoreceptor differentiation Inferred from genetic interaction PubMed 15376326. Source: MGI negative regulation of neuroblast proliferation Inferred from direct assay PubMed 12798291. Source: MGI negative regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 15869495PubMed 8861722. Source: MGI nerve development Inferred from mutant phenotype PubMed 7605630. Source: MGI neuron recognition Inferred from mutant phenotype PubMed 15844198. Source: MGI positive regulation of neuron differentiation Inferred from direct assay PubMed 12798291. Source: MGI regulation of metabolic process Inferred from mutant phenotype PubMed 12106019. Source: MGI regulation of retinal cell programmed cell death Inferred from direct assay PubMed 11747369. Source: MGI regulation of synaptic plasticity Inferred from direct assay PubMed 16819522PubMed 17442827. Source: MGI response to drug Inferred from mutant phenotype PubMed 15249684. Source: MGI ureteric bud development Inferred from direct assay PubMed 16018995. Source: MGI
Cellular_component	cytoplasmic membrane-bounded vesicle Inferred from direct assay PubMed 14715936PubMed 15242649. Source: MGI endoplasmic reticulum lumen Traceable author statement. Source: Reactome extracellular region Traceable author statement. Source: Reactome perinuclear region of cytoplasm Inferred from direct assay PubMed 22209827. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 130	112			PRO_0000019635
	Chain	131 – 249	119	Brain-derived neurotrophic factor		PRO_0000019636
Sites
	Site	57 – 58	2	Cleavage; by S1P By similarity
Amino acid modifications
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Disulfide bond	143 ↔ 210		By similarity
	Disulfide bond	188 ↔ 239		By similarity
	Disulfide bond	198 ↔ 241		By similarity
Experimental info
	Mutagenesis	68	1	V → M: Reduced hippocampal volume, dendritic complexity and context-dependent memory. Increased anxiety-related behaviors. Ref.5
	Mutagenesis	130	1	R → K: Forms homodimers processed at L-112 that seem more stable than the wild-type. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P21237 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 90CE1F16BB235C97	FASTA	249	28,123
	10 20 30 40 50 60 MTILFLTMVI SYFGCMKAAP MKEVNVHGQG NLAYPGVRTH GTLESVNGPR AGSRGLTTTS 70 80 90 100 110 120 LADTFEHVIE ELLDEDQKVR PNEENHKDAD LYTSRVMLSS QVPLEPPLLF LLEEYKNYLD 130 140 150 160 170 180 AANMSMRVRR HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK 190 200 210 220 230 240 QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain." Hofer M., Pagliusi S.R., Hohn A., Leibrock J., Barde Y.-A. EMBO J. 9:2459-2464(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[3]	"Characterisation of neurotrophin dimers and monomers." Kolbeck R., Jungbluth S., Barde Y.-A. Eur. J. Biochem. 225:995-1003(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 112-121 AND 131-135, SUBUNIT, MUTAGENESIS OF ARG-130.
[4]	"Cleavage of proBDNF by tPA/plasmin is essential for long-term hippocampal plasticity." Pang P.T., Teng H.K., Zaitsev E., Woo N.T., Sakata K., Zhen S., Teng K.K., Yung W.-H., Hempstead B.L., Lu B. Science 306:487-491(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ACTIVATION BY PLG.
[5]	"Genetic variant BDNF (Val66Met) polymorphism alters anxiety-related behavior." Chen Z.-Y., Jing D., Bath K.G., Ieraci A., Khan T., Siao C.-J., Herrera D.G., Toth M., Yang C., McEwen B.S., Hempstead B.L., Lee F.S. Science 314:140-143(2006) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF VAL-68.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55573 mRNA. Translation: CAA39159.1. BC034862 mRNA. Translation: AAH34862.1.
IPI	IPI00121215.
PIR	S12555.
RefSeq	NP_001041604.1. NM_001048139.1. NP_001041606.1. NM_001048141.1. NP_001041607.1. NM_001048142.1.
UniGene	Mm.1442.
3D structure databases
ProteinModelPortal	P21237.
SMR	P21237. Positions 138-246.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5724N.
PTM databases
PhosphoSite	P21237.
Proteomic databases
PRIDE	P21237.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000111043; ENSMUSP00000106672; ENSMUSG00000048482. ENSMUST00000111044; ENSMUSP00000106673; ENSMUSG00000048482. ENSMUST00000111045; ENSMUSP00000106674; ENSMUSG00000048482. ENSMUST00000111046; ENSMUSP00000106675; ENSMUSG00000048482. ENSMUST00000111047; ENSMUSP00000106676; ENSMUSG00000048482. ENSMUST00000111049; ENSMUSP00000106678; ENSMUSG00000048482. ENSMUST00000111050; ENSMUSP00000106679; ENSMUSG00000048482. ENSMUST00000111051; ENSMUSP00000106680; ENSMUSG00000048482. ENSMUST00000176893; ENSMUSP00000135762; ENSMUSG00000048482.
GeneID	12064.
KEGG	mmu:12064.
Organism-specific databases
CTD	627.
MGI	MGI:88145. Bdnf.
Phylogenomic databases
eggNOG	NOG45248.
HOGENOM	HOG000231516.
HOVERGEN	HBG006494.
InParanoid	P21237.
KO	K04355.
OrthoDB	EOG4R503P.
Enzyme and pathway databases
Reactome	REACT_115202. Signal Transduction.
Gene expression databases
ArrayExpress	P21237.
Bgee	P21237.
CleanEx	MM_BDNF.
Genevestigator	P21237.
GermOnline	ENSMUSG00000048482. Mus musculus.
Family and domain databases
InterPro	IPR020430. Brain-der_neurotrophic_factor. IPR020408. Nerve_growth_factor-like. IPR002072. Nerve_growth_factor-rel. IPR019846. Nerve_growth_factor_CS. [Graphical view]
PANTHER	PTHR11589. PTHR11589. 1 hit.
Pfam	PF00243. NGF. 1 hit. [Graphical view]
PIRSF	PIRSF001789. NGF. 1 hit.
PRINTS	PR01912. BDNFACTOR. PR00268. NGF.
ProDom	PD002052. Nerve_growth_factor-rel. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00140. NGF. 1 hit. [Graphical view]
PROSITE	PS00248. NGF_1. 1 hit. PS50270. NGF_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280377.
SOURCE	Search...

Entry information

Entry name

BDNF_MOUSE

Accession

Primary (citable) accession number: P21237

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program