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Protein

Brain-derived neurotrophic factor

Gene

Bdnf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 582Cleavage; by S1PBy similarity

GO - Molecular functioni

GO - Biological processi

  • axon extension Source: MGI
  • axon guidance Source: MGI
  • axon target recognition Source: MGI
  • behavioral fear response Source: MGI
  • cell-cell signaling Source: GO_Central
  • circadian rhythm Source: UniProtKB
  • dendrite development Source: MGI
  • dendrite extension Source: MGI
  • fear response Source: MGI
  • feeding behavior Source: MGI
  • gamma-aminobutyric acid signaling pathway Source: MGI
  • glutamate secretion Source: MGI
  • inner ear development Source: MGI
  • learning or memory Source: MGI
  • mechanoreceptor differentiation Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cell death Source: MGI
  • negative regulation of neuroblast proliferation Source: MGI
  • negative regulation of neuron apoptotic process Source: MGI
  • negative regulation of synaptic transmission, GABAergic Source: MGI
  • nerve development Source: MGI
  • neuron projection morphogenesis Source: GO_Central
  • neuron recognition Source: MGI
  • positive regulation of glucocorticoid receptor signaling pathway Source: MGI
  • positive regulation of neuron differentiation Source: MGI
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • positive regulation of synapse assembly Source: MGI
  • regulation of inhibitory postsynaptic membrane potential Source: MGI
  • regulation of metabolic process Source: MGI
  • regulation of neuron apoptotic process Source: MGI
  • regulation of retinal cell programmed cell death Source: MGI
  • regulation of synaptic plasticity Source: MGI
  • response to drug Source: MGI
  • taste bud development Source: MGI
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
  • ureteric bud development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Enzyme and pathway databases

ReactomeiREACT_289095. NRAGE signals death through JNK.
REACT_317611. NGF processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Brain-derived neurotrophic factor
Short name:
BDNF
Gene namesi
Name:Bdnf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88145. Bdnf.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic membrane-bounded vesicle Source: MGI
  • endoplasmic reticulum lumen Source: Reactome
  • extracellular region Source: Reactome
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi68 – 681V → M: Reduced hippocampal volume, dendritic complexity and context-dependent memory. Increased anxiety-related behaviors. 1 Publication
Mutagenesisi130 – 1301R → K: Forms homodimers processed at L-112 that seem more stable than the wild-type. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Propeptidei19 – 1301121 PublicationPRO_0000019635Add
BLAST
Chaini131 – 249119Brain-derived neurotrophic factorPRO_0000019636Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi143 ↔ 210By similarity
Disulfide bondi188 ↔ 239By similarity
Disulfide bondi198 ↔ 241By similarity

Post-translational modificationi

Converted into mature BDNF by plasmin (PLG).

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP21237.
PRIDEiP21237.

PTM databases

PhosphoSiteiP21237.

Expressioni

Inductioni

Expression oscillates in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain. Expressed at higher levels during the dark period and at lower levels during the light period.1 Publication

Gene expression databases

BgeeiP21237.
CleanExiMM_BDNF.
ExpressionAtlasiP21237. baseline and differential.
GenevisibleiP21237. MM.

Interactioni

Subunit structurei

Monomers and homodimers. Binds to NTRK2/TRKB.1 Publication

Protein-protein interaction databases

DIPiDIP-5724N.
IntActiP21237. 2 interactions.
STRINGi10090.ENSMUSP00000057989.

Structurei

3D structure databases

ProteinModelPortaliP21237.
SMRiP21237. Positions 138-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45248.
HOGENOMiHOG000231516.
HOVERGENiHBG006494.
InParanoidiP21237.
KOiK04355.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR020430. Brain-der_neurotrophic_factor.
IPR029034. Cystine-knot_cytokine.
IPR020408. Nerve_growth_factor-like.
IPR002072. Nerve_growth_factor-rel.
IPR019846. Nerve_growth_factor_CS.
[Graphical view]
PANTHERiPTHR11589. PTHR11589. 1 hit.
PfamiPF00243. NGF. 1 hit.
[Graphical view]
PIRSFiPIRSF001789. NGF. 1 hit.
PRINTSiPR01912. BDNFACTOR.
PR00268. NGF.
ProDomiPD002052. Nerve_growth_factor-rel. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00140. NGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00248. NGF_1. 1 hit.
PS50270. NGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTILFLTMVI SYFGCMKAAP MKEVNVHGQG NLAYPGVRTH GTLESVNGPR
60 70 80 90 100
AGSRGLTTTS LADTFEHVIE ELLDEDQKVR PNEENHKDAD LYTSRVMLSS
110 120 130 140 150
QVPLEPPLLF LLEEYKNYLD AANMSMRVRR HSDPARRGEL SVCDSISEWV
160 170 180 190 200
TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG
210 220 230 240
IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR
Length:249
Mass (Da):28,123
Last modified:May 1, 1991 - v1
Checksum:i90CE1F16BB235C97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55573 mRNA. Translation: CAA39159.1.
BC034862 mRNA. Translation: AAH34862.1.
CCDSiCCDS38194.1.
PIRiS12555.
RefSeqiNP_001041604.1. NM_001048139.1.
NP_001041606.1. NM_001048141.1.
NP_001041607.1. NM_001048142.1.
NP_001272345.1. NM_001285416.1.
NP_001272346.1. NM_001285417.1.
NP_001272347.1. NM_001285418.1.
NP_001272348.1. NM_001285419.1.
NP_001272349.1. NM_001285420.1.
NP_001272350.1. NM_001285421.1.
NP_001272351.1. NM_001285422.1.
UniGeneiMm.1442.

Genome annotation databases

EnsembliENSMUST00000111043; ENSMUSP00000106672; ENSMUSG00000048482.
ENSMUST00000111044; ENSMUSP00000106673; ENSMUSG00000048482.
ENSMUST00000111045; ENSMUSP00000106674; ENSMUSG00000048482.
ENSMUST00000111046; ENSMUSP00000106675; ENSMUSG00000048482.
ENSMUST00000111047; ENSMUSP00000106676; ENSMUSG00000048482.
ENSMUST00000111049; ENSMUSP00000106678; ENSMUSG00000048482.
ENSMUST00000111050; ENSMUSP00000106679; ENSMUSG00000048482.
ENSMUST00000111051; ENSMUSP00000106680; ENSMUSG00000048482.
ENSMUST00000176893; ENSMUSP00000135762; ENSMUSG00000048482.
GeneIDi12064.
KEGGimmu:12064.
UCSCiuc008lme.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55573 mRNA. Translation: CAA39159.1.
BC034862 mRNA. Translation: AAH34862.1.
CCDSiCCDS38194.1.
PIRiS12555.
RefSeqiNP_001041604.1. NM_001048139.1.
NP_001041606.1. NM_001048141.1.
NP_001041607.1. NM_001048142.1.
NP_001272345.1. NM_001285416.1.
NP_001272346.1. NM_001285417.1.
NP_001272347.1. NM_001285418.1.
NP_001272348.1. NM_001285419.1.
NP_001272349.1. NM_001285420.1.
NP_001272350.1. NM_001285421.1.
NP_001272351.1. NM_001285422.1.
UniGeneiMm.1442.

3D structure databases

ProteinModelPortaliP21237.
SMRiP21237. Positions 138-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-5724N.
IntActiP21237. 2 interactions.
STRINGi10090.ENSMUSP00000057989.

PTM databases

PhosphoSiteiP21237.

Proteomic databases

MaxQBiP21237.
PRIDEiP21237.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111043; ENSMUSP00000106672; ENSMUSG00000048482.
ENSMUST00000111044; ENSMUSP00000106673; ENSMUSG00000048482.
ENSMUST00000111045; ENSMUSP00000106674; ENSMUSG00000048482.
ENSMUST00000111046; ENSMUSP00000106675; ENSMUSG00000048482.
ENSMUST00000111047; ENSMUSP00000106676; ENSMUSG00000048482.
ENSMUST00000111049; ENSMUSP00000106678; ENSMUSG00000048482.
ENSMUST00000111050; ENSMUSP00000106679; ENSMUSG00000048482.
ENSMUST00000111051; ENSMUSP00000106680; ENSMUSG00000048482.
ENSMUST00000176893; ENSMUSP00000135762; ENSMUSG00000048482.
GeneIDi12064.
KEGGimmu:12064.
UCSCiuc008lme.1. mouse.

Organism-specific databases

CTDi627.
MGIiMGI:88145. Bdnf.

Phylogenomic databases

eggNOGiNOG45248.
HOGENOMiHOG000231516.
HOVERGENiHBG006494.
InParanoidiP21237.
KOiK04355.

Enzyme and pathway databases

ReactomeiREACT_289095. NRAGE signals death through JNK.
REACT_317611. NGF processing.

Miscellaneous databases

NextBioi280377.
PROiP21237.
SOURCEiSearch...

Gene expression databases

BgeeiP21237.
CleanExiMM_BDNF.
ExpressionAtlasiP21237. baseline and differential.
GenevisibleiP21237. MM.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR020430. Brain-der_neurotrophic_factor.
IPR029034. Cystine-knot_cytokine.
IPR020408. Nerve_growth_factor-like.
IPR002072. Nerve_growth_factor-rel.
IPR019846. Nerve_growth_factor_CS.
[Graphical view]
PANTHERiPTHR11589. PTHR11589. 1 hit.
PfamiPF00243. NGF. 1 hit.
[Graphical view]
PIRSFiPIRSF001789. NGF. 1 hit.
PRINTSiPR01912. BDNFACTOR.
PR00268. NGF.
ProDomiPD002052. Nerve_growth_factor-rel. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00140. NGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00248. NGF_1. 1 hit.
PS50270. NGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain."
    Hofer M., Pagliusi S.R., Hohn A., Leibrock J., Barde Y.-A.
    EMBO J. 9:2459-2464(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  3. "Characterisation of neurotrophin dimers and monomers."
    Kolbeck R., Jungbluth S., Barde Y.-A.
    Eur. J. Biochem. 225:995-1003(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 112-121 AND 131-135, SUBUNIT, MUTAGENESIS OF ARG-130.
  4. "Cleavage of proBDNF by tPA/plasmin is essential for long-term hippocampal plasticity."
    Pang P.T., Teng H.K., Zaitsev E., Woo N.T., Sakata K., Zhen S., Teng K.K., Yung W.-H., Hempstead B.L., Lu B.
    Science 306:487-491(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVATION BY PLG.
  5. "Genetic variant BDNF (Val66Met) polymorphism alters anxiety-related behavior."
    Chen Z.-Y., Jing D., Bath K.G., Ieraci A., Khan T., Siao C.-J., Herrera D.G., Toth M., Yang C., McEwen B.S., Hempstead B.L., Lee F.S.
    Science 314:140-143(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF VAL-68.
  6. "p75 neurotrophin receptor is a clock gene that regulates oscillatory components of circadian and metabolic networks."
    Baeza-Raja B., Eckel-Mahan K., Zhang L., Vagena E., Tsigelny I.F., Sassone-Corsi P., Ptacek L.J., Akassoglou K.
    J. Neurosci. 33:10221-10234(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiBDNF_MOUSE
AccessioniPrimary (citable) accession number: P21237
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: June 24, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.