P21236 (ERM_STRPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
rRNA adenine N-6-methyltransferase
EC=2.1.1.184

Alternative name(s):
ErmAM
Macrolide-lincosamide-streptogramin B resistance protein

Gene names

Name:

erm

Organism

Streptococcus pneumoniae

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity	2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA.
Sequence similarities	Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Transposable element
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 245

245

rRNA adenine N-6-methyltransferase

PRO_0000101690

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

245

Helix Strand Turn

Details...

Turn

15 – 17

3

Helix

18 – 24

7

Beta strand

29 – 35

7

Helix

45 – 50

6

Beta strand

51 – 60

10

Beta strand

62 – 68

7

Turn

70 – 73

4

Beta strand

75 – 79

5

Turn

85 – 88

4

Beta strand

92 – 100

9

Beta strand

103 – 105

3

Helix

107 – 116

10

Beta strand

120 – 129

10

Helix

130 – 135

6

Helix

137 – 139

3

Helix

141 – 144

4

Turn

145 – 148

4

Beta strand

153 – 157

5

Beta strand

162 – 165

4

Beta strand

170 – 175

6

Helix

184 – 199

16

Helix

202 – 205

4

Beta strand

207 – 209

3

Helix

210 – 217

8

Helix

229 – 241

13

Sequences

	Sequence		Length	Mass (Da)
	P21236 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 5E3AB1BAAA6511C5	FASTA	245	28,783
	10 20 30 40 50 60 MNKNIKYSQN FLTSEKVLNQ IIKQLNLKET DTVYEIGTGK GHLTTKLAKI SKQVTSIELD 70 80 90 100 110 120 SHLFNLSSEK LKLNIRVTLI HQDILQFQFP NKQRYKIVGS IPYHLSTQII KKVVFESHAS 130 140 150 160 170 180 DIYLIVEEGF YKRTLDIHRT LGLLLHTQVS IQQLLKLPAE CFHPKPKVNS VLIKLTRHTT 190 200 210 220 230 240 DVPDKYWKLY TYFVSKWVNR EYRQLFTKNQ FHQAMKHAKV NNLSTITYEQ VLSIFNSYLL FNGRK

References

[1]	"Nucleotide sequence of the erythromycin resistance gene of the conjugative transposon Tn1545." Trieu-Cuot P., Poyart-Salmeron C., Carlier C., Courvalin P. Nucleic Acids Res. 18:3660-3660(1990) [PubMed: 2163525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: Tn1545.
[2]	"Solution structure of an rRNA methyltransferase (ErmAM) that confers macrolide-lincosamide-streptogramin antibiotic resistance." Yu L., Petros A.M., Schnuchel A., Zhong P., Severin J.M., Walter K., Holzman T.F., Fesik S.W. Nat. Struct. Biol. 4:483-489(1997) [PubMed: 9187657] [Abstract] Cited for: STRUCTURE BY NMR.
[3]	Erratum Yu L., Petros A.M., Schnuchel A., Zhong P., Severin J.M., Walter K., Holzman T.F., Fesik S.W. Nat. Struct. Biol. 4:592-592(1997)
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X52632 Genomic DNA. No translation available.

PIR

S12727.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YUB	NMR	-	A	1-245	[»]

ProteinModelPortal

P21236.

SMR

P21236. Positions 1-245.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
[Graphical view]

Gene3D

G3DSA:1.10.8.100. rRNA_Ade_diMease-like. 1 hit.

PANTHER

PTHR11727. RRNA_meth_trans. 1 hit.

Pfam

PF00398. RrnaAD. 1 hit.
[Graphical view]

SMART

SM00650. rADc. 1 hit.
[Graphical view]

PROSITE

PS01131. RRNA_A_DIMETH. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ERM_STRPN

Accession

Primary (citable) accession number: P21236

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	May 31, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program