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P21226 (CHI2_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endochitinase A2

EC=3.2.1.14
Gene names
Name:CHI2
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Defense against chitin containing fungal pathogens.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Induction

By infection with the fungal pathogen Ascochyta pisi.

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Contains 1 chitin-binding type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.2
Chain21 – 309289Endochitinase A2
PRO_0000005308
Propeptide310 – 32415Removed in mature form By similarity
PRO_0000005309

Regions

Domain21 – 6141Chitin-binding type-1

Amino acid modifications

Disulfide bond23 ↔ 38 By similarity
Disulfide bond32 ↔ 44 By similarity
Disulfide bond37 ↔ 51 By similarity
Disulfide bond55 ↔ 59 By similarity
Disulfide bond151 ↔ 170 By similarity
Disulfide bond269 ↔ 301 By similarity

Sequences

Sequence LengthMass (Da)Tools
P21226 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 540F0DA5EC1DC2FA

FASTA32434,678
        10         20         30         40         50         60 
MSKLRIPILL VLFIVSCCSA EQCGTQAGGA LCPGGLCCSK FGWCGSTSEY CGDGCQSQCS 

        70         80         90        100        110        120 
GSSGGGTLSS LISGDTFNNM LKHRNDNACQ GKPFYTYDAF LSAAKAFPNF ANKGDTATKK 

       130        140        150        160        170        180 
REIAAFLGQT SHETTGGWPT APDGPYAWGY CFLREQNPST YCQASSEFPC ASGKQYYGRG 

       190        200        210        220        230        240 
PIQISWNYNY GQCGRAIGVD LLNNPDLVAT DPVISFKTAL WFWMTPQSPK PSCHDVITGG 

       250        260        270        280        290        300 
WTPSSADRAA GRLPGYGTVT NIINGGLECG RGQDSRVQDR IGFYKRYCDI FGIGYGDNLD 

       310        320 
CYSQRPFGSS LPLSSILLDT VAAA 

« Hide

References

[1]"Molecular cloning and characterization of a pea chitinase gene expressed in response to wounding, fungal infection and the elicitor chitosan."
Chang M.M., Horovitz D., Culley D., Hadwiger L.A.
Plant Mol. Biol. 28:105-111(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Alcan.
[2]"Induction, purification and characterization of chitinase isolated from pea leaves inoculated with Ascochyta pisi."
Vad K., Mikkelsen J.D., Collinge D.B.
Planta 184:24-29(1991)
Cited for: PROTEIN SEQUENCE OF 21-36.
Strain: cv. Birte.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L37876 Genomic DNA. Translation: AAA75196.1.
PIRS56694.

3D structure databases

ProteinModelPortalP21226.
SMRP21226. Positions 21-307.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.60.10. 1 hit.
InterProIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
PRINTSPR00451. CHITINBINDNG.
SMARTSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHI2_PEA
AccessionPrimary (citable) accession number: P21226
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries