Reviewed, UniProtKB/Swiss-Prot P21226 (CHI2_PEA)

Names and origin

Protein names

Recommended name:
    Endochitinase A2
    EC=3.2.1.14

Gene names

Name:

CHI2

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Induction	By infection with the fungal pathogen Ascochyta pisi.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.2
	Chain	21 – 309	289	Endochitinase A2		PRO_0000005308
	Propeptide	310 – 324	15	Removed in mature form By similarity		PRO_0000005309
Regions
	Domain	21 – 61	41	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	23 ↔ 38		By similarity
	Disulfide bond	32 ↔ 44		By similarity
	Disulfide bond	37 ↔ 51		By similarity
	Disulfide bond	55 ↔ 59		By similarity
	Disulfide bond	151 ↔ 170		By similarity
	Disulfide bond	269 ↔ 301		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21226-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 540F0DA5EC1DC2FA	FASTA	324	34,678
	10 20 30 40 50 60 MSKLRIPILL VLFIVSCCSA EQCGTQAGGA LCPGGLCCSK FGWCGSTSEY CGDGCQSQCS 70 80 90 100 110 120 GSSGGGTLSS LISGDTFNNM LKHRNDNACQ GKPFYTYDAF LSAAKAFPNF ANKGDTATKK 130 140 150 160 170 180 REIAAFLGQT SHETTGGWPT APDGPYAWGY CFLREQNPST YCQASSEFPC ASGKQYYGRG 190 200 210 220 230 240 PIQISWNYNY GQCGRAIGVD LLNNPDLVAT DPVISFKTAL WFWMTPQSPK PSCHDVITGG 250 260 270 280 290 300 WTPSSADRAA GRLPGYGTVT NIINGGLECG RGQDSRVQDR IGFYKRYCDI FGIGYGDNLD 310 320 CYSQRPFGSS LPLSSILLDT VAAA

References

[1]	"Molecular cloning and characterization of a pea chitinase gene expressed in response to wounding, fungal infection and the elicitor chitosan." Chang M.M., Horovitz D., Culley D., Hadwiger L.A. Plant Mol. Biol. 28:105-111(1995) [PubMed: 7787175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Alcan.
[2]	"Induction, purification and characterization of chitinase isolated from pea leaves inoculated with Ascochyta pisi." Vad K., Mikkelsen J.D., Collinge D.B. Planta 184:24-29(1991) Cited for: PROTEIN SEQUENCE OF 21-36. Strain: cv. Birte. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L37876 Genomic DNA. Translation: AAA75196.1.
PIR	S56694.
3D structure databases
HSSP	HSSP built from PDB template 1HEV based on UniProtKB P02877.
SMR	P21226. Positions 21-307.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 287.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI2_PEA

Accession

Primary (citable) accession number: P21226

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)