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P21226

- CHI2_PEA

UniProt

P21226 - CHI2_PEA

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Protein
Endochitinase A2
Gene
CHI2
Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Defense against chitin containing fungal pathogens.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW
  2. chitinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response Source: UniProtKB-KW
  4. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase A2 (EC:3.2.1.14)
Gene namesi
Name:CHI2
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 Publication
Add
BLAST
Chaini21 – 309289Endochitinase A2
PRO_0000005308Add
BLAST
Propeptidei310 – 32415Removed in mature form By similarity
PRO_0000005309Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi23 ↔ 38 By similarity
Disulfide bondi32 ↔ 44 By similarity
Disulfide bondi37 ↔ 51 By similarity
Disulfide bondi55 ↔ 59 By similarity
Disulfide bondi151 ↔ 170 By similarity
Disulfide bondi269 ↔ 301 By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By infection with the fungal pathogen Ascochyta pisi.

Structurei

3D structure databases

ProteinModelPortaliP21226.
SMRiP21226. Positions 21-307.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 6141Chitin-binding type-1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21226-1 [UniParc]FASTAAdd to Basket

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MSKLRIPILL VLFIVSCCSA EQCGTQAGGA LCPGGLCCSK FGWCGSTSEY    50
CGDGCQSQCS GSSGGGTLSS LISGDTFNNM LKHRNDNACQ GKPFYTYDAF 100
LSAAKAFPNF ANKGDTATKK REIAAFLGQT SHETTGGWPT APDGPYAWGY 150
CFLREQNPST YCQASSEFPC ASGKQYYGRG PIQISWNYNY GQCGRAIGVD 200
LLNNPDLVAT DPVISFKTAL WFWMTPQSPK PSCHDVITGG WTPSSADRAA 250
GRLPGYGTVT NIINGGLECG RGQDSRVQDR IGFYKRYCDI FGIGYGDNLD 300
CYSQRPFGSS LPLSSILLDT VAAA 324
Length:324
Mass (Da):34,678
Last modified:October 1, 1996 - v2
Checksum:i540F0DA5EC1DC2FA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L37876 Genomic DNA. Translation: AAA75196.1.
PIRiS56694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L37876 Genomic DNA. Translation: AAA75196.1 .
PIRi S56694.

3D structure databases

ProteinModelPortali P21226.
SMRi P21226. Positions 21-307.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.60.10. 1 hit.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view ]
PIRSFi PIRSF001060. Endochitinase. 1 hit.
PRINTSi PR00451. CHITINBINDNG.
SMARTi SM00270. ChtBD1. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of a pea chitinase gene expressed in response to wounding, fungal infection and the elicitor chitosan."
    Chang M.M., Horovitz D., Culley D., Hadwiger L.A.
    Plant Mol. Biol. 28:105-111(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Alcan.
  2. "Induction, purification and characterization of chitinase isolated from pea leaves inoculated with Ascochyta pisi."
    Vad K., Mikkelsen J.D., Collinge D.B.
    Planta 184:24-29(1991)
    Cited for: PROTEIN SEQUENCE OF 21-36.
    Strain: cv. Birte.
    Tissue: Leaf.

Entry informationi

Entry nameiCHI2_PEA
AccessioniPrimary (citable) accession number: P21226
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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