P21219 (NHA1_RHORH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High-molecular weight cobalt-containing nitrile hydratase subunit alpha
Short name=H-NHase
Short name=H-nitrilase
EC=4.2.1.84

Gene names

Name:

nhhA

Organism

Rhodococcus rhodochrous

Taxonomic identifier

1829 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NHase catalyzes the hydration of various nitrile compounds to the corresponding amides.
Catalytic activity	An aliphatic amide = a nitrile + H2O.
Cofactor	Binds 1 Co3+ ion per subunit.
Subunit structure	Heterodimer of an alpha and a beta chain.
Induction	By cobalt and urea or cyclohexanecarboxamide.
Biotechnological use	Industrial production of acrylamide is now being developed using some of these enzymes.
Sequence similarities	Belongs to the nitrile hydratase subunit alpha family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	indole-3-acetonitrile nitrile hydratase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.3
	Chain	2 – 203	202	High-molecular weight cobalt-containing nitrile hydratase subunit alpha		PRO_0000186824
Sites
	Metal binding	102	1	Cobalt By similarity
	Metal binding	105	1	Cobalt By similarity
	Metal binding	106	1	Cobalt By similarity
	Metal binding	107	1	Cobalt By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21219 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 515ED2A4863E6C98	FASTA	203	22,835
	10 20 30 40 50 60 MSEHVNKYTE YEARTKAIET LLYERGLITP AAVDRVVSYY ENEIGPMGGA KVVAKSWVDP 70 80 90 100 110 120 EYRKWLEEDA TAAMASLGYA GEQAHQISAV FNDSQTHHVV VCTLCSCYPW PVLGLPPAWY 130 140 150 160 170 180 KSMEYRSRVV ADPRGVLKRD FGFDIPDEVE VRVWDSSSEI RYIVIPERPA GTDGWSEEEL 190 200 TKLVSRDSMI GVSNALTPQE VIV

References

[1]	"Cloning, nucleotide sequence and expression in Escherichia coli of two cobalt-containing nitrile hydratase genes from Rhodococcus rhodochrous J1." Kobayashi M., Nishiyama M., Nagasawa T., Horinouchi S., Beppu T., Yamada H. Biochim. Biophys. Acta 1129:23-33(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-27 AND 162-173. Strain: J1.
[2]	"Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1." Komeda H., Kobayashi M., Shimizu S. Proc. Natl. Acad. Sci. U.S.A. 93:4267-4272(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: J1.
[3]	"Characterization of a new cobalt-containing nitrile hydratase purified from urea-induced cells of Rhodococcus rhodochrous J1." Nagasawa T., Takeuchi K., Yamada H. Eur. J. Biochem. 196:581-589(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-29. Strain: J1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64359 Genomic DNA. Translation: CAA45710.1. D67027 Genomic DNA. Translation: BAA11044.1.
PIR	S19714.
3D structure databases
ProteinModelPortal	P21219.
SMR	P21219. Positions 9-195.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-2283.
Family and domain databases
Gene3D	3.90.330.10. 1 hit.
InterPro	IPR004232. CN_Hdrtase_a/SCN_Hdrlase_g. IPR023900. CN_Hdrtase_asu/SCN_Hdrlase_gsu. IPR018141. Nitrile_hydratase_asu. [Graphical view]
Pfam	PF02979. NHase_alpha. 1 hit. [Graphical view]
PIRSF	PIRSF001426. NHase_alpha. 1 hit.
ProDom	PD007559. CN_Hdrtase_asu/SCN_Hdrlase_gsu. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56209. Nitrile_hydratase_asu. 1 hit.
TIGRFAMs	TIGR01323. nitrile_alph. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NHA1_RHORH

Accession

Primary (citable) accession number: P21219

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program