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Reviewed, UniProtKB/Swiss-Prot P21218 (PORB_ARATH)

Last modified June 16, 2009. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Protochlorophyllide reductase B, chloroplastic
      Short name=PCR B
    EC=1.3.1.33
Alternative name(s):
    NADPH-protochlorophyllide oxidoreductase B
      Short name=POR B
Gene names
Name: PORB
Ordered Locus Names: At4g27440
ORF Names: F27G19.40
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).

Catalytic activity

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathway

Porphyrin biosynthesis; chlorophyll biosynthesis.

Subcellular location

Plastid › chloroplast outer membrane. Plastid › chloroplast thylakoid membrane. Ref.5

Tissue specificity

Expressed in seedlings and adult plant.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6666Chloroplast Potential
Chain67 – 401335Protochlorophyllide reductase B, chloroplastic
PRO_0000023288

Natural variations

Natural variant3951E → D in strain: cv. An-2.

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Sequences

Sequence LengthMass (Da)Tools
P21218-1 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 0C2132F980AF6CA3

FASTA40143,359
        10         20         30         40         50         60 
MALQAASLVS SAFSVRKDAK LNASSSSFKD SSLFGASITD QIKSEHGSSS LRFKREQSLR 

        70         80         90        100        110        120 
NLAIRAQTAA TSSPTVTKSV DGKKTLRKGN VVVTGASSGL GLATAKALAE TGKWNVIMAC 

       130        140        150        160        170        180 
RDFLKAERAA KSVGMPKDSY TVMHLDLASL DSVRQFVDNF RRTETPLDVL VCNAAVYFPT 

       190        200        210        220        230        240 
AKEPTYSAEG FELSVATNHL GHFLLARLLL DDLKKSDYPS KRLIIVGSIT GNTNTLAGNV 

       250        260        270        280        290        300 
PPKANLGDLR GLAGGLNGLN SSAMIDGGDF DGAKAYKDSK VCNMLTMQEF HRRFHEETGV 

       310        320        330        340        350        360 
TFASLYPGCI ASTGLFREHI PLFRALFPPF QKYITKGYVS ETESGKRLAQ VVSDPSLTKS 

       370        380        390        400 
GVYWSWNNAS ASFENQLSEE ASDVEKARKV WEISEKLVGL A

« Hide

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References

« Hide 'large scale' references
[1]"Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana."
Armstrong G.A., Runge S., Frick G., Sperling U., Apel K.
Plant Physiol. 108:1505-1517(1995) [PubMed: 7659751] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Effect of light on the NADPH-protochlorophyllide oxidoreductase of Arabidopsis thaliana."
Benli M., Schuelz R., Apel K.
Plant Mol. Biol. 16:615-625(1991) [PubMed: 1714319] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE.
Strain: cv. An-2.
Tissue: Leaf.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

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Cross-references

Sequence databases

U29785 mRNA. Translation: AAC49044.1.
AL078467 Genomic DNA. Translation: CAB43876.1.
AL161571 Genomic DNA. Translation: CAB81394.1.
AY042883 mRNA. Translation: AAK68823.1.
AY054206 mRNA. Translation: AAL06867.1.
AY081465 mRNA. Translation: AAM10027.1.
IPIIPI00549013.
PIRT08936.
RefSeqNP_001031731.1.
NP_194474.1.
UniGeneAt.23416
At.71042

3D structure databases

HSSPHSSP built from PDB template 1FDS based on UniProtKB P14061.
ModBaseSearch...

Proteomic databases

PRIDEP21218.

Genome annotation databases

GeneID828853.
GenomeReviewsGene locus AT4G27440 in contig CT486007_GR.
KEGGath:AT4G27440.
NMPDRfig|3702.1.peg.20681.

Organism-specific databases

TAIRAt4g27440.

Enzyme and pathway databases

BRENDA1.3.1.33. 302.

Gene expression databases

ArrayExpressP21218.
GermOnlineAT4G27440. Arabidopsis thaliana.

Family and domain databases

InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR19410. ADH_short_C2. 1 hit.
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
TIGRFAMsTIGR01289. LPOR. 1 hit.
ProtoNetSearch...

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Entry information

Entry namePORB_ARATH
AccessionPrimary (citable) accession number: P21218
Secondary accession number(s): Q42537
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1997
Last modified: June 16, 2009
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents