Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protochlorophyllide reductase B, chloroplastic

Gene

PORB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).2 Publications

Miscellaneous

The presence of TOC33 is required for the import of PORA into plastids in cotyledons and true leaves.1 Publication

Catalytic activityi

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • protein domain specific binding Source: CAFA
  • protochlorophyllide reductase activity Source: UniProtKB-EC

GO - Biological processi

  • chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  • photosynthesis Source: UniProtKB-KW
  • response to ethylene Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processChlorophyll biosynthesis, Photosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT4G27440-MONOMER
BRENDAi1.3.1.33 399
UniPathwayiUPA00668

Names & Taxonomyi

Protein namesi
Recommended name:
Protochlorophyllide reductase B, chloroplastic (EC:1.3.1.33)
Short name:
PCR B
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase B
Short name:
POR B
Gene namesi
Name:PORB
Ordered Locus Names:At4g27440
ORF Names:F27G19.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G27440
TAIRilocus:2124044 AT4G27440

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid outer membrane, Thylakoid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype at the levels of the whole plant or chloroplast ultrastructure; due to the redundancy with PORC. Porb and porc double mutants have a seedling-lethal pale-yellow xantha phenotype at the cotyledon stage, contain only small amounts of Chla, and possess chloroplasts with mostly unstacked thylakoid membranes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 66ChloroplastSequence analysisCombined sourcesAdd BLAST66
ChainiPRO_000002328867 – 401Protochlorophyllide reductase B, chloroplasticAdd BLAST335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67Pyrrolidone carboxylic acidCombined sources1

Keywords - PTMi

Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP21218
PRIDEiP21218

PTM databases

iPTMnetiP21218

Expressioni

Tissue specificityi

Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds. Expressed in seedlings and adult plant.1 Publication

Developmental stagei

Expressed in etiolated seedlings.1 Publication

Inductioni

Circadian-regulation. Not regulated by light.3 Publications

Gene expression databases

ExpressionAtlasiP21218 baseline and differential
GenevisibleiP21218 AT

Interactioni

Subunit structurei

Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1 (PubMed:22212719). Interacts with CDF1 in chloroplast (PubMed:24151298).2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi14140, 3 interactors
IntActiP21218, 3 interactors
MINTiP21218
STRINGi3702.AT4G27440.1

Structurei

3D structure databases

ProteinModelPortaliP21218
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1208 Eukaryota
COG1028 LUCA
InParanoidiP21218
KOiK00218
OMAiQFVANVR
OrthoDBiEOG09360CR9
PhylomeDBiP21218

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR005979 Prochl_reduct
IPR002347 SDR_fam
PANTHERiPTHR44419 PTHR44419, 1 hit
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
SUPFAMiSSF51735 SSF51735, 2 hits
TIGRFAMsiTIGR01289 LPOR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQAASLVS SAFSVRKDAK LNASSSSFKD SSLFGASITD QIKSEHGSSS
60 70 80 90 100
LRFKREQSLR NLAIRAQTAA TSSPTVTKSV DGKKTLRKGN VVVTGASSGL
110 120 130 140 150
GLATAKALAE TGKWNVIMAC RDFLKAERAA KSVGMPKDSY TVMHLDLASL
160 170 180 190 200
DSVRQFVDNF RRTETPLDVL VCNAAVYFPT AKEPTYSAEG FELSVATNHL
210 220 230 240 250
GHFLLARLLL DDLKKSDYPS KRLIIVGSIT GNTNTLAGNV PPKANLGDLR
260 270 280 290 300
GLAGGLNGLN SSAMIDGGDF DGAKAYKDSK VCNMLTMQEF HRRFHEETGV
310 320 330 340 350
TFASLYPGCI ASTGLFREHI PLFRALFPPF QKYITKGYVS ETESGKRLAQ
360 370 380 390 400
VVSDPSLTKS GVYWSWNNAS ASFENQLSEE ASDVEKARKV WEISEKLVGL

A
Length:401
Mass (Da):43,359
Last modified:November 1, 1997 - v3
Checksum:i0C2132F980AF6CA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47G → R in AAM65116 (Ref. 7) Curated1
Sequence conflicti110E → Q in AAM65116 (Ref. 7) Curated1
Sequence conflicti231G → A in AAM65116 (Ref. 7) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti395E → D in strain: cv. An-2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29785 mRNA Translation: AAC49044.1
AL078467 Genomic DNA Translation: CAB43876.1
AL161571 Genomic DNA Translation: CAB81394.1
CP002687 Genomic DNA Translation: AEE85342.1
CP002687 Genomic DNA Translation: AEE85343.1
AY042883 mRNA Translation: AAK68823.1
AY054206 mRNA Translation: AAL06867.1
AY081465 mRNA Translation: AAM10027.1
AK317009 mRNA Translation: BAH19703.1
AY087574 mRNA Translation: AAM65116.1
PIRiT08936
RefSeqiNP_001031731.1, NM_001036654.1
NP_194474.1, NM_118879.4
UniGeneiAt.23416
At.75221

Genome annotation databases

EnsemblPlantsiAT4G27440.1; AT4G27440.1; AT4G27440
AT4G27440.2; AT4G27440.2; AT4G27440
GeneIDi828853
GrameneiAT4G27440.1; AT4G27440.1; AT4G27440
AT4G27440.2; AT4G27440.2; AT4G27440
KEGGiath:AT4G27440

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPORB_ARATH
AccessioniPrimary (citable) accession number: P21218
Secondary accession number(s): B9DG36, Q42537, Q8LAV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health