P21218 (PORB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protochlorophyllide reductase B, chloroplastic
Short name=PCR B
EC=1.3.1.33

Alternative name(s):
NADPH-protochlorophyllide oxidoreductase B
Short name=POR B

Gene names

Name:	PORB
Ordered Locus Names:	At4g27440
ORF Names:	F27G19.40

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).
Catalytic activity	Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast outer membrane. Plastid › chloroplast thylakoid membrane Ref.6.
Tissue specificity	Expressed in seedlings and adult plant.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Membrane Plastid Plastid outer membrane Thylakoid
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW response to ethylene stimulus Inferred from expression pattern PubMed 19948955. Source: TAIR
Cellular_component	chloroplast envelope Inferred from direct assay Ref.6PubMed 12938931PubMed 20061580. Source: TAIR chloroplast outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast thylakoid membrane Inferred from direct assay PubMed 15322131. Source: TAIR
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro protochlorophyllide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 66	66	Chloroplast Potential
	Chain	67 – 401	335	Protochlorophyllide reductase B, chloroplastic		PRO_0000023288
Natural variations
	Natural variant	395	1	E → D in strain: cv. An-2.

Sequences

	Sequence		Length	Mass (Da)
	P21218 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 0C2132F980AF6CA3	FASTA	401	43,359
	10 20 30 40 50 60 MALQAASLVS SAFSVRKDAK LNASSSSFKD SSLFGASITD QIKSEHGSSS LRFKREQSLR 70 80 90 100 110 120 NLAIRAQTAA TSSPTVTKSV DGKKTLRKGN VVVTGASSGL GLATAKALAE TGKWNVIMAC 130 140 150 160 170 180 RDFLKAERAA KSVGMPKDSY TVMHLDLASL DSVRQFVDNF RRTETPLDVL VCNAAVYFPT 190 200 210 220 230 240 AKEPTYSAEG FELSVATNHL GHFLLARLLL DDLKKSDYPS KRLIIVGSIT GNTNTLAGNV 250 260 270 280 290 300 PPKANLGDLR GLAGGLNGLN SSAMIDGGDF DGAKAYKDSK VCNMLTMQEF HRRFHEETGV 310 320 330 340 350 360 TFASLYPGCI ASTGLFREHI PLFRALFPPF QKYITKGYVS ETESGKRLAQ VVSDPSLTKS 370 380 390 400 GVYWSWNNAS ASFENQLSEE ASDVEKARKV WEISEKLVGL A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana." Armstrong G.A., Runge S., Frick G., Sperling U., Apel K. Plant Physiol. 108:1505-1517(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Effect of light on the NADPH-protochlorophyllide oxidoreductase of Arabidopsis thaliana." Benli M., Schuelz R., Apel K. Plant Mol. Biol. 16:615-625(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE. Strain: cv. An-2. Tissue: Leaf.
[3]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana." Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N. Mol. Cell. Proteomics 2:325-345(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: cv. Wassilewskija.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29785 mRNA. Translation: AAC49044.1. AL078467 Genomic DNA. Translation: CAB43876.1. AL161571 Genomic DNA. Translation: CAB81394.1. CP002687 Genomic DNA. Translation: AEE85342.1. CP002687 Genomic DNA. Translation: AEE85343.1. AY042883 mRNA. Translation: AAK68823.1. AY054206 mRNA. Translation: AAL06867.1. AY081465 mRNA. Translation: AAM10027.1.
IPI	IPI00549013.
PIR	T08936.
RefSeq	NP_001031731.1. NM_001036654.1. NP_194474.1. NM_118879.3.
UniGene	At.23416. At.75221.
3D structure databases
ProteinModelPortal	P21218.
SMR	P21218. Positions 91-294.
ModBase	Search...
Protein-protein interaction databases
IntAct	P21218. 1 interaction.
STRING	3702.AT4G27440.1-P.
Proteomic databases
PaxDb	P21218.
PRIDE	P21218.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G27440.1; AT4G27440.1; AT4G27440. AT4G27440.2; AT4G27440.2; AT4G27440.
GeneID	828853.
KEGG	ath:AT4G27440.
Organism-specific databases
TAIR	At4g27440.
Phylogenomic databases
eggNOG	COG1028.
InParanoid	P21218.
KO	K00218.
OMA	WNNASAS.
PhylomeDB	P21218.
ProtClustDB	PLN00015.
Enzyme and pathway databases
BRENDA	1.3.1.33. 399.
UniPathway	UPA00668.
Gene expression databases
ArrayExpress	P21218.
Genevestigator	P21218.
GermOnline	AT4G27440. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR005979. Prochl_reduct. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01289. LPOR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PORB_ARATH

Accession

Primary (citable) accession number: P21218
Secondary accession number(s): Q42537

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 126 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program