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P21218 (PORB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protochlorophyllide reductase B, chloroplastic

Short name=PCR B
EC=1.3.1.33
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase B
Short name=POR B
Gene names
Name:PORB
Ordered Locus Names:At4g27440
ORF Names:F27G19.40
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide). Ref.8 Ref.10

Catalytic activity

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathway

Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Subunit structure

Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1. Ref.13

Subcellular location

Plastidchloroplast outer membrane. Plastidchloroplast thylakoid membrane Ref.11 Ref.12 Ref.13.

Tissue specificity

Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds. Expressed in seedlings and adult plant. Ref.9

Developmental stage

Expressed in etiolated seedlings. Ref.10

Induction

Circadian-regulation. Not regulated by light. Ref.1 Ref.9 Ref.10

Disruption phenotype

No visible phenotype at the levels of the whole plant or chloroplast ultrastructure; due to the redundancy with PORC. Porb and porc double mutants have a seedling-lethal pale-yellow xantha phenotype at the cotyledon stage, contain only small amounts of Chla, and possess chloroplasts with mostly unstacked thylakoid membranes. Ref.8

Miscellaneous

The presence of TOC33 is required for the import of PORA into plastids in cotyledons and true leaves (Ref.12).

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6666Chloroplast Potential
Chain67 – 401335Protochlorophyllide reductase B, chloroplastic
PRO_0000023288

Natural variations

Natural variant3951E → D in strain: cv. An-2.

Experimental info

Sequence conflict471G → R in AAM65116. Ref.7
Sequence conflict1101E → Q in AAM65116. Ref.7
Sequence conflict2311G → A in AAM65116. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P21218 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 0C2132F980AF6CA3

FASTA40143,359
        10         20         30         40         50         60 
MALQAASLVS SAFSVRKDAK LNASSSSFKD SSLFGASITD QIKSEHGSSS LRFKREQSLR 

        70         80         90        100        110        120 
NLAIRAQTAA TSSPTVTKSV DGKKTLRKGN VVVTGASSGL GLATAKALAE TGKWNVIMAC 

       130        140        150        160        170        180 
RDFLKAERAA KSVGMPKDSY TVMHLDLASL DSVRQFVDNF RRTETPLDVL VCNAAVYFPT 

       190        200        210        220        230        240 
AKEPTYSAEG FELSVATNHL GHFLLARLLL DDLKKSDYPS KRLIIVGSIT GNTNTLAGNV 

       250        260        270        280        290        300 
PPKANLGDLR GLAGGLNGLN SSAMIDGGDF DGAKAYKDSK VCNMLTMQEF HRRFHEETGV 

       310        320        330        340        350        360 
TFASLYPGCI ASTGLFREHI PLFRALFPPF QKYITKGYVS ETESGKRLAQ VVSDPSLTKS 

       370        380        390        400 
GVYWSWNNAS ASFENQLSEE ASDVEKARKV WEISEKLVGL A 

« Hide

References

« Hide 'large scale' references
[1]"Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana."
Armstrong G.A., Runge S., Frick G., Sperling U., Apel K.
Plant Physiol. 108:1505-1517(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY LIGHT.
Strain: cv. Columbia.
[2]"Effect of light on the NADPH-protochlorophyllide oxidoreductase of Arabidopsis thaliana."
Benli M., Schuelz R., Apel K.
Plant Mol. Biol. 16:615-625(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. An-2.
Tissue: Leaf.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"An Arabidopsis porB porC double mutant lacking light-dependent NADPH:protochlorophyllide oxidoreductases B and C is highly chlorophyll-deficient and developmentally arrested."
Frick G., Su Q., Apel K., Armstrong G.A.
Plant J. 35:141-153(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[9]"Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana."
Oosawa N., Masuda T., Awai K., Fusada N., Shimada H., Ohta H., Takamiya K.
FEBS Lett. 474:133-136(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION BY LIGHT.
Strain: cv. Columbia.
[10]"POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light."
Su Q., Frick G., Armstrong G., Apel K.
Plant Mol. Biol. 47:805-813(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY LIGHT, DEVELOPMENTAL STAGE.
Strain: cv. Columbia.
[11]"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
Mol. Cell. Proteomics 2:325-345(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Wassilewskija.
[12]"Multiplicity of different cell- and organ-specific import routes for the NADPH-protochlorophyllide oxidoreductases A and B in plastids of Arabidopsis seedlings."
Kim C., Ham H., Apel K.
Plant J. 42:329-340(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[13]"FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U29785 mRNA. Translation: AAC49044.1.
AL078467 Genomic DNA. Translation: CAB43876.1.
AL161571 Genomic DNA. Translation: CAB81394.1.
CP002687 Genomic DNA. Translation: AEE85342.1.
CP002687 Genomic DNA. Translation: AEE85343.1.
AY042883 mRNA. Translation: AAK68823.1.
AY054206 mRNA. Translation: AAL06867.1.
AY081465 mRNA. Translation: AAM10027.1.
AK317009 mRNA. Translation: BAH19703.1.
AY087574 mRNA. Translation: AAM65116.1.
PIRT08936.
RefSeqNP_001031731.1. NM_001036654.1.
NP_194474.1. NM_118879.3.
UniGeneAt.23416.
At.75221.

3D structure databases

ProteinModelPortalP21218.
SMRP21218. Positions 83-401.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid14140. 3 interactions.
IntActP21218. 3 interactions.
MINTMINT-8300916.
STRING3702.AT4G27440.1-P.

Proteomic databases

PaxDbP21218.
PRIDEP21218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G27440.1; AT4G27440.1; AT4G27440.
AT4G27440.2; AT4G27440.2; AT4G27440.
GeneID828853.
KEGGath:AT4G27440.

Organism-specific databases

TAIRAT4G27440.

Phylogenomic databases

eggNOGCOG1028.
InParanoidP21218.
KOK00218.
OMAPGCIAST.
PhylomeDBP21218.
ProtClustDBPLN00015.

Enzyme and pathway databases

BioCycARA:AT4G27440-MONOMER.
ARA:GQT-2099-MONOMER.
BRENDA1.3.1.33. 399.
UniPathwayUPA00668.

Gene expression databases

ArrayExpressP21218.
GenevestigatorP21218.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
TIGRFAMsTIGR01289. LPOR. 1 hit.
ProtoNetSearch...

Entry information

Entry namePORB_ARATH
AccessionPrimary (citable) accession number: P21218
Secondary accession number(s): B9DG36, Q42537, Q8LAV9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names