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Protein

Protochlorophyllide reductase B, chloroplastic

Gene

PORB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).2 Publications

Catalytic activityi

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathway:ichlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

  • chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  • photosynthesis Source: UniProtKB-KW
  • response to ethylene Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT4G27440-MONOMER.
ARA:GQT-2099-MONOMER.
BRENDAi1.3.1.33. 399.
UniPathwayiUPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protochlorophyllide reductase B, chloroplastic (EC:1.3.1.33)
Short name:
PCR B
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase B
Short name:
POR B
Gene namesi
Name:PORB
Ordered Locus Names:At4g27440
ORF Names:F27G19.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G27440.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast outer membrane Source: UniProtKB-SubCell
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid outer membrane, Thylakoid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype at the levels of the whole plant or chloroplast ultrastructure; due to the redundancy with PORC. Porb and porc double mutants have a seedling-lethal pale-yellow xantha phenotype at the cotyledon stage, contain only small amounts of Chla, and possess chloroplasts with mostly unstacked thylakoid membranes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6666ChloroplastSequence AnalysisAdd
BLAST
Chaini67 – 401335Protochlorophyllide reductase B, chloroplasticPRO_0000023288Add
BLAST

Proteomic databases

PaxDbiP21218.
PRIDEiP21218.

Expressioni

Tissue specificityi

Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds. Expressed in seedlings and adult plant.1 Publication

Developmental stagei

Expressed in etiolated seedlings.1 Publication

Inductioni

Circadian-regulation. Not regulated by light.3 Publications

Interactioni

Subunit structurei

Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1 (PubMed:22212719). Interacts with CDF1 in chloroplast (PubMed:24151298).2 Publications

Protein-protein interaction databases

BioGridi14140. 3 interactions.
IntActiP21218. 3 interactions.
MINTiMINT-8300916.
STRINGi3702.AT4G27440.1.

Structurei

3D structure databases

ProteinModelPortaliP21218.
SMRiP21218. Positions 91-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiP21218.
KOiK00218.
OMAiRFKREHS.
PhylomeDBiP21218.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
TIGRFAMsiTIGR01289. LPOR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQAASLVS SAFSVRKDAK LNASSSSFKD SSLFGASITD QIKSEHGSSS
60 70 80 90 100
LRFKREQSLR NLAIRAQTAA TSSPTVTKSV DGKKTLRKGN VVVTGASSGL
110 120 130 140 150
GLATAKALAE TGKWNVIMAC RDFLKAERAA KSVGMPKDSY TVMHLDLASL
160 170 180 190 200
DSVRQFVDNF RRTETPLDVL VCNAAVYFPT AKEPTYSAEG FELSVATNHL
210 220 230 240 250
GHFLLARLLL DDLKKSDYPS KRLIIVGSIT GNTNTLAGNV PPKANLGDLR
260 270 280 290 300
GLAGGLNGLN SSAMIDGGDF DGAKAYKDSK VCNMLTMQEF HRRFHEETGV
310 320 330 340 350
TFASLYPGCI ASTGLFREHI PLFRALFPPF QKYITKGYVS ETESGKRLAQ
360 370 380 390 400
VVSDPSLTKS GVYWSWNNAS ASFENQLSEE ASDVEKARKV WEISEKLVGL

A
Length:401
Mass (Da):43,359
Last modified:November 1, 1997 - v3
Checksum:i0C2132F980AF6CA3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471G → R in AAM65116 (Ref. 7) Curated
Sequence conflicti110 – 1101E → Q in AAM65116 (Ref. 7) Curated
Sequence conflicti231 – 2311G → A in AAM65116 (Ref. 7) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti395 – 3951E → D in strain: cv. An-2.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29785 mRNA. Translation: AAC49044.1.
AL078467 Genomic DNA. Translation: CAB43876.1.
AL161571 Genomic DNA. Translation: CAB81394.1.
CP002687 Genomic DNA. Translation: AEE85342.1.
CP002687 Genomic DNA. Translation: AEE85343.1.
AY042883 mRNA. Translation: AAK68823.1.
AY054206 mRNA. Translation: AAL06867.1.
AY081465 mRNA. Translation: AAM10027.1.
AK317009 mRNA. Translation: BAH19703.1.
AY087574 mRNA. Translation: AAM65116.1.
PIRiT08936.
RefSeqiNP_001031731.1. NM_001036654.1.
NP_194474.1. NM_118879.3.
UniGeneiAt.23416.
At.75221.

Genome annotation databases

EnsemblPlantsiAT4G27440.1; AT4G27440.1; AT4G27440.
AT4G27440.2; AT4G27440.2; AT4G27440.
GeneIDi828853.
KEGGiath:AT4G27440.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29785 mRNA. Translation: AAC49044.1.
AL078467 Genomic DNA. Translation: CAB43876.1.
AL161571 Genomic DNA. Translation: CAB81394.1.
CP002687 Genomic DNA. Translation: AEE85342.1.
CP002687 Genomic DNA. Translation: AEE85343.1.
AY042883 mRNA. Translation: AAK68823.1.
AY054206 mRNA. Translation: AAL06867.1.
AY081465 mRNA. Translation: AAM10027.1.
AK317009 mRNA. Translation: BAH19703.1.
AY087574 mRNA. Translation: AAM65116.1.
PIRiT08936.
RefSeqiNP_001031731.1. NM_001036654.1.
NP_194474.1. NM_118879.3.
UniGeneiAt.23416.
At.75221.

3D structure databases

ProteinModelPortaliP21218.
SMRiP21218. Positions 91-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14140. 3 interactions.
IntActiP21218. 3 interactions.
MINTiMINT-8300916.
STRINGi3702.AT4G27440.1.

Proteomic databases

PaxDbiP21218.
PRIDEiP21218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G27440.1; AT4G27440.1; AT4G27440.
AT4G27440.2; AT4G27440.2; AT4G27440.
GeneIDi828853.
KEGGiath:AT4G27440.

Organism-specific databases

TAIRiAT4G27440.

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiP21218.
KOiK00218.
OMAiRFKREHS.
PhylomeDBiP21218.

Enzyme and pathway databases

UniPathwayiUPA00668.
BioCyciARA:AT4G27440-MONOMER.
ARA:GQT-2099-MONOMER.
BRENDAi1.3.1.33. 399.

Miscellaneous databases

PROiP21218.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
TIGRFAMsiTIGR01289. LPOR. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana."
    Armstrong G.A., Runge S., Frick G., Sperling U., Apel K.
    Plant Physiol. 108:1505-1517(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY LIGHT.
    Strain: cv. Columbia.
  2. "Effect of light on the NADPH-protochlorophyllide oxidoreductase of Arabidopsis thaliana."
    Benli M., Schuelz R., Apel K.
    Plant Mol. Biol. 16:615-625(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANT ASP-395.
    Strain: cv. An-2.
    Tissue: Leaf.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "An Arabidopsis porB porC double mutant lacking light-dependent NADPH:protochlorophyllide oxidoreductases B and C is highly chlorophyll-deficient and developmentally arrested."
    Frick G., Su Q., Apel K., Armstrong G.A.
    Plant J. 35:141-153(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana."
    Oosawa N., Masuda T., Awai K., Fusada N., Shimada H., Ohta H., Takamiya K.
    FEBS Lett. 474:133-136(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION BY LIGHT.
    Strain: cv. Columbia.
  10. "POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light."
    Su Q., Frick G., Armstrong G., Apel K.
    Plant Mol. Biol. 47:805-813(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY LIGHT, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
  11. "Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
    Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
    Mol. Cell. Proteomics 2:325-345(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Wassilewskija.
  12. "Multiplicity of different cell- and organ-specific import routes for the NADPH-protochlorophyllide oxidoreductases A and B in plastids of Arabidopsis seedlings."
    Kim C., Ham H., Apel K.
    Plant J. 42:329-340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  13. "FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
    Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
    FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.
  14. "Cell growth defect factor1/chaperone-like protein of POR1 plays a role in stabilization of light-dependent protochlorophyllide oxidoreductase in Nicotiana benthamiana and Arabidopsis."
    Lee J.-Y., Lee H.-S., Song J.-Y., Jung Y.J., Reinbothe S., Park Y.-I., Lee S.Y., Pai H.-S.
    Plant Cell 25:3944-3960(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDF1.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPORB_ARATH
AccessioniPrimary (citable) accession number: P21218
Secondary accession number(s): B9DG36, Q42537, Q8LAV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The presence of TOC33 is required for the import of PORA into plastids in cotyledons and true leaves.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.