Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P21218

- PORB_ARATH

UniProt

P21218 - PORB_ARATH

Protein

Protochlorophyllide reductase B, chloroplastic

Gene

PORB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).2 Publications

    Catalytic activityi

    Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. protochlorophyllide reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
    2. photosynthesis Source: UniProtKB-KW
    3. response to ethylene Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Photosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciARA:AT4G27440-MONOMER.
    ARA:GQT-2099-MONOMER.
    BRENDAi1.3.1.33. 399.
    UniPathwayiUPA00668.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protochlorophyllide reductase B, chloroplastic (EC:1.3.1.33)
    Short name:
    PCR B
    Alternative name(s):
    NADPH-protochlorophyllide oxidoreductase B
    Short name:
    POR B
    Gene namesi
    Name:PORB
    Ordered Locus Names:At4g27440
    ORF Names:F27G19.40
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G27440.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. chloroplast outer membrane Source: UniProtKB-SubCell
    4. chloroplast thylakoid Source: TAIR
    5. chloroplast thylakoid membrane Source: TAIR
    6. membrane Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Plastid outer membrane, Thylakoid

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype at the levels of the whole plant or chloroplast ultrastructure; due to the redundancy with PORC. Porb and porc double mutants have a seedling-lethal pale-yellow xantha phenotype at the cotyledon stage, contain only small amounts of Chla, and possess chloroplasts with mostly unstacked thylakoid membranes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6666ChloroplastSequence AnalysisAdd
    BLAST
    Chaini67 – 401335Protochlorophyllide reductase B, chloroplasticPRO_0000023288Add
    BLAST

    Proteomic databases

    PaxDbiP21218.
    PRIDEiP21218.

    Expressioni

    Tissue specificityi

    Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds. Expressed in seedlings and adult plant.1 Publication

    Developmental stagei

    Expressed in etiolated seedlings.1 Publication

    Inductioni

    Circadian-regulation. Not regulated by light.3 Publications

    Gene expression databases

    ArrayExpressiP21218.
    GenevestigatoriP21218.

    Interactioni

    Subunit structurei

    Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1.1 Publication

    Protein-protein interaction databases

    BioGridi14140. 3 interactions.
    IntActiP21218. 3 interactions.
    MINTiMINT-8300916.
    STRINGi3702.AT4G27440.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP21218.
    SMRiP21218. Positions 85-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1028.
    InParanoidiP21218.
    KOiK00218.
    OMAiPGCIAST.
    PhylomeDBiP21218.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR005979. Prochl_reduct.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    TIGRFAMsiTIGR01289. LPOR. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21218-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALQAASLVS SAFSVRKDAK LNASSSSFKD SSLFGASITD QIKSEHGSSS    50
    LRFKREQSLR NLAIRAQTAA TSSPTVTKSV DGKKTLRKGN VVVTGASSGL 100
    GLATAKALAE TGKWNVIMAC RDFLKAERAA KSVGMPKDSY TVMHLDLASL 150
    DSVRQFVDNF RRTETPLDVL VCNAAVYFPT AKEPTYSAEG FELSVATNHL 200
    GHFLLARLLL DDLKKSDYPS KRLIIVGSIT GNTNTLAGNV PPKANLGDLR 250
    GLAGGLNGLN SSAMIDGGDF DGAKAYKDSK VCNMLTMQEF HRRFHEETGV 300
    TFASLYPGCI ASTGLFREHI PLFRALFPPF QKYITKGYVS ETESGKRLAQ 350
    VVSDPSLTKS GVYWSWNNAS ASFENQLSEE ASDVEKARKV WEISEKLVGL 400
    A 401
    Length:401
    Mass (Da):43,359
    Last modified:November 1, 1997 - v3
    Checksum:i0C2132F980AF6CA3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471G → R in AAM65116. 1 PublicationCurated
    Sequence conflicti110 – 1101E → Q in AAM65116. 1 PublicationCurated
    Sequence conflicti231 – 2311G → A in AAM65116. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti395 – 3951E → D in strain: cv. An-2.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U29785 mRNA. Translation: AAC49044.1.
    AL078467 Genomic DNA. Translation: CAB43876.1.
    AL161571 Genomic DNA. Translation: CAB81394.1.
    CP002687 Genomic DNA. Translation: AEE85342.1.
    CP002687 Genomic DNA. Translation: AEE85343.1.
    AY042883 mRNA. Translation: AAK68823.1.
    AY054206 mRNA. Translation: AAL06867.1.
    AY081465 mRNA. Translation: AAM10027.1.
    AK317009 mRNA. Translation: BAH19703.1.
    AY087574 mRNA. Translation: AAM65116.1.
    PIRiT08936.
    RefSeqiNP_001031731.1. NM_001036654.1.
    NP_194474.1. NM_118879.3.
    UniGeneiAt.23416.
    At.75221.

    Genome annotation databases

    EnsemblPlantsiAT4G27440.1; AT4G27440.1; AT4G27440.
    AT4G27440.2; AT4G27440.2; AT4G27440.
    GeneIDi828853.
    KEGGiath:AT4G27440.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U29785 mRNA. Translation: AAC49044.1 .
    AL078467 Genomic DNA. Translation: CAB43876.1 .
    AL161571 Genomic DNA. Translation: CAB81394.1 .
    CP002687 Genomic DNA. Translation: AEE85342.1 .
    CP002687 Genomic DNA. Translation: AEE85343.1 .
    AY042883 mRNA. Translation: AAK68823.1 .
    AY054206 mRNA. Translation: AAL06867.1 .
    AY081465 mRNA. Translation: AAM10027.1 .
    AK317009 mRNA. Translation: BAH19703.1 .
    AY087574 mRNA. Translation: AAM65116.1 .
    PIRi T08936.
    RefSeqi NP_001031731.1. NM_001036654.1.
    NP_194474.1. NM_118879.3.
    UniGenei At.23416.
    At.75221.

    3D structure databases

    ProteinModelPortali P21218.
    SMRi P21218. Positions 85-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 14140. 3 interactions.
    IntActi P21218. 3 interactions.
    MINTi MINT-8300916.
    STRINGi 3702.AT4G27440.1-P.

    Proteomic databases

    PaxDbi P21218.
    PRIDEi P21218.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G27440.1 ; AT4G27440.1 ; AT4G27440 .
    AT4G27440.2 ; AT4G27440.2 ; AT4G27440 .
    GeneIDi 828853.
    KEGGi ath:AT4G27440.

    Organism-specific databases

    TAIRi AT4G27440.

    Phylogenomic databases

    eggNOGi COG1028.
    InParanoidi P21218.
    KOi K00218.
    OMAi PGCIAST.
    PhylomeDBi P21218.

    Enzyme and pathway databases

    UniPathwayi UPA00668 .
    BioCyci ARA:AT4G27440-MONOMER.
    ARA:GQT-2099-MONOMER.
    BRENDAi 1.3.1.33. 399.

    Gene expression databases

    ArrayExpressi P21218.
    Genevestigatori P21218.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR005979. Prochl_reduct.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    TIGRFAMsi TIGR01289. LPOR. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana."
      Armstrong G.A., Runge S., Frick G., Sperling U., Apel K.
      Plant Physiol. 108:1505-1517(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY LIGHT.
      Strain: cv. Columbia.
    2. "Effect of light on the NADPH-protochlorophyllide oxidoreductase of Arabidopsis thaliana."
      Benli M., Schuelz R., Apel K.
      Plant Mol. Biol. 16:615-625(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: cv. An-2.
      Tissue: Leaf.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "An Arabidopsis porB porC double mutant lacking light-dependent NADPH:protochlorophyllide oxidoreductases B and C is highly chlorophyll-deficient and developmentally arrested."
      Frick G., Su Q., Apel K., Armstrong G.A.
      Plant J. 35:141-153(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    9. "Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana."
      Oosawa N., Masuda T., Awai K., Fusada N., Shimada H., Ohta H., Takamiya K.
      FEBS Lett. 474:133-136(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION BY LIGHT.
      Strain: cv. Columbia.
    10. "POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light."
      Su Q., Frick G., Armstrong G., Apel K.
      Plant Mol. Biol. 47:805-813(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY LIGHT, DEVELOPMENTAL STAGE.
      Strain: cv. Columbia.
    11. "Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
      Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
      Mol. Cell. Proteomics 2:325-345(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Wassilewskija.
    12. "Multiplicity of different cell- and organ-specific import routes for the NADPH-protochlorophyllide oxidoreductases A and B in plastids of Arabidopsis seedlings."
      Kim C., Ham H., Apel K.
      Plant J. 42:329-340(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    13. "FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
      Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
      FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.

    Entry informationi

    Entry nameiPORB_ARATH
    AccessioniPrimary (citable) accession number: P21218
    Secondary accession number(s): B9DG36, Q42537, Q8LAV9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 139 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The presence of TOC33 is required for the import of PORA into plastids in cotyledons and true leaves.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3