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P21217

- FUT3_HUMAN

UniProt

P21217 - FUT3_HUMAN

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Protein

Galactoside 3(4)-L-fucosyltransferase

Gene

FUT3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.

Catalytic activityi

GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->4))-N-acetyl-beta-D-glucosaminyl-R.

Pathwayi

GO - Molecular functioni

  1. 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity Source: UniProtKB-EC
  2. alpha-(1->3)-fucosyltransferase activity Source: BHF-UCL
  3. fucosyltransferase activity Source: BHF-UCL

GO - Biological processi

  1. cell-cell recognition Source: BHF-UCL
  2. fucosylation Source: GOC
  3. macromolecule glycosylation Source: BHF-UCL
  4. oligosaccharide biosynthetic process Source: BHF-UCL
  5. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Blood group antigen, Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.65. 2681.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT10. Glycosyltransferase Family 10.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactoside 3(4)-L-fucosyltransferase (EC:2.4.1.65)
Alternative name(s):
Blood group Lewis alpha-4-fucosyltransferase
Short name:
Lewis FT
Fucosyltransferase 3
Fucosyltransferase III
Short name:
FucT-III
Gene namesi
Name:FUT3
Synonyms:FT3B, LE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:4014. FUT3.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein
Note: Membrane-bound form in trans cisternae of Golgi.

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

MIMi111100. gene+phenotype.
PharmGKBiPA28430.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Galactoside 3(4)-L-fucosyltransferasePRO_0000221096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi154 – 1541N-linked (GlcNAc...)Curated
Glycosylationi185 – 1851N-linked (GlcNAc...)Curated

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP21217.
PRIDEiP21217.

PTM databases

PhosphoSiteiP21217.

Expressioni

Tissue specificityi

Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.

Gene expression databases

BgeeiP21217.
CleanExiHS_FUT3.
ExpressionAtlasiP21217. baseline and differential.
GenevestigatoriP21217.

Organism-specific databases

HPAiHPA046966.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000305603.

Structurei

3D structure databases

ProteinModelPortaliP21217.
SMRiP21217. Positions 187-325.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini35 – 361327LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3419Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 10 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG283180.
GeneTreeiENSGT00680000099679.
HOGENOMiHOG000045583.
HOVERGENiHBG000274.
InParanoidiP21217.
KOiK00716.
OrthoDBiEOG7RJPS6.
PhylomeDBiP21217.
TreeFamiTF316348.

Family and domain databases

InterProiIPR001503. Glyco_trans_10.
[Graphical view]
PANTHERiPTHR11929. PTHR11929. 1 hit.
PfamiPF00852. Glyco_transf_10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21217 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPLGAAKPQ WPWRRCLAAL LFQLLVAVCF FSYLRVSRDD ATGSPRAPSG
60 70 80 90 100
SSRQDTTPTR PTLLILLWTW PFHIPVALSR CSEMVPGTAD CHITADRKVY
110 120 130 140 150
PQADTVIVHH WDIMSNPKSR LPPSPRPQGQ RWIWFNLEPP PNCQHLEALD
160 170 180 190 200
RYFNLTMSYR SDSDIFTPYG WLEPWSGQPA HPPLNLSAKT ELVAWAVSNW
210 220 230 240 250
KPDSARVRYY QSLQAHLKVD VYGRSHKPLP KGTMMETLSR YKFYLAFENS
260 270 280 290 300
LHPDYITEKL WRNALEAWAV PVVLGPSRSN YERFLPPDAF IHVDDFQSPK
310 320 330 340 350
DLARYLQELD KDHARYLSYF RWRETLRPRS FSWALDFCKA CWKLQQESRY
360
QTVRSIAAWF T
Length:361
Mass (Da):42,117
Last modified:May 1, 1991 - v1
Checksum:iBF4398044F19C284
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti5 – 51G → S.1 Publication
Corresponds to variant rs28362458 [ dbSNP | Ensembl ].
VAR_022200
Natural varianti20 – 201L → R in Le(-). 5 Publications
Corresponds to variant rs28362459 [ dbSNP | Ensembl ].
VAR_003426
Natural varianti68 – 681W → R in Le(-). 5 Publications
Corresponds to variant rs812936 [ dbSNP | Ensembl ].
VAR_007959
Natural varianti102 – 1021Q → K in Le(+). 1 Publication
Corresponds to variant rs59796499 [ dbSNP | Ensembl ].
VAR_007960
Natural varianti105 – 1051T → M in Le(-). 6 Publications
Corresponds to variant rs778986 [ dbSNP | Ensembl ].
VAR_003427
Natural varianti124 – 1241S → A in Le(+). 1 Publication
VAR_007961
Natural varianti160 – 1601R → C.1 Publication
Corresponds to variant rs28362462 [ dbSNP | Ensembl ].
VAR_022201
Natural varianti162 – 1621D → N in Le(-). 2 Publications
Corresponds to variant rs28362463 [ dbSNP | Ensembl ].
VAR_007962
Natural varianti170 – 1701G → S in Le(-); completely inactive. 3 Publications
Corresponds to variant rs28362464 [ dbSNP | Ensembl ].
VAR_003428
Natural varianti223 – 2231G → R in Le(-). 2 Publications
Corresponds to variant rs28362466 [ dbSNP | Ensembl ].
VAR_007963
Natural varianti270 – 2701V → M in Le(-). 2 Publications
Corresponds to variant rs28381968 [ dbSNP | Ensembl ].
VAR_007964
Natural varianti325 – 3251T → M.1 Publication
Corresponds to variant rs28381969 [ dbSNP | Ensembl ].
VAR_022202
Natural varianti327 – 3271R → Q.1 Publication
Corresponds to variant rs28381970 [ dbSNP | Ensembl ].
VAR_022203
Natural varianti336 – 3361D → A in Le(-). 1 Publication
VAR_003429
Natural varianti356 – 3561I → K in Le(-); less than 10% reduction in activity. 3 Publications
Corresponds to variant rs3894326 [ dbSNP | Ensembl ].
VAR_003430

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53578 mRNA. Translation: CAA37641.1.
U27326 mRNA. Translation: AAC50185.1.
U27327 mRNA. Translation: AAC50186.1.
U27328 mRNA. Translation: AAC50187.1.
D89324 Genomic DNA. Translation: BAA13941.1.
D89325 Genomic DNA. Translation: BAA13942.1.
AF131913 mRNA. Translation: AAD33514.1.
FM210024 Genomic DNA. Translation: CAR64692.1.
FM210025 Genomic DNA. Translation: CAR64693.1.
AY870341 Genomic DNA. Translation: AAW34365.1.
AC024592 Genomic DNA. No translation available.
BC074836 mRNA. Translation: AAH74836.1.
BC074837 mRNA. Translation: AAH74837.1.
BC108675 mRNA. Translation: AAI08676.1.
CCDSiCCDS12153.1.
PIRiA36669.
RefSeqiNP_000140.1. NM_000149.3.
NP_001091108.1. NM_001097639.1.
NP_001091109.1. NM_001097640.1.
NP_001091110.1. NM_001097641.1.
UniGeneiHs.169238.

Genome annotation databases

EnsembliENST00000303225; ENSP00000305603; ENSG00000171124.
ENST00000458379; ENSP00000416443; ENSG00000171124.
ENST00000589620; ENSP00000465804; ENSG00000171124.
ENST00000589918; ENSP00000468123; ENSG00000171124.
GeneIDi2525.
KEGGihsa:2525.
UCSCiuc002mdj.2. human.

Polymorphism databases

DMDMi121137.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

dbRBC/BGMUT

Blood group antigen gene mutation database

GGDB

GlycoGene database

SeattleSNPs
Functional Glycomics Gateway - GTase

Fucosyltransferase 3

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53578 mRNA. Translation: CAA37641.1 .
U27326 mRNA. Translation: AAC50185.1 .
U27327 mRNA. Translation: AAC50186.1 .
U27328 mRNA. Translation: AAC50187.1 .
D89324 Genomic DNA. Translation: BAA13941.1 .
D89325 Genomic DNA. Translation: BAA13942.1 .
AF131913 mRNA. Translation: AAD33514.1 .
FM210024 Genomic DNA. Translation: CAR64692.1 .
FM210025 Genomic DNA. Translation: CAR64693.1 .
AY870341 Genomic DNA. Translation: AAW34365.1 .
AC024592 Genomic DNA. No translation available.
BC074836 mRNA. Translation: AAH74836.1 .
BC074837 mRNA. Translation: AAH74837.1 .
BC108675 mRNA. Translation: AAI08676.1 .
CCDSi CCDS12153.1.
PIRi A36669.
RefSeqi NP_000140.1. NM_000149.3.
NP_001091108.1. NM_001097639.1.
NP_001091109.1. NM_001097640.1.
NP_001091110.1. NM_001097641.1.
UniGenei Hs.169238.

3D structure databases

ProteinModelPortali P21217.
SMRi P21217. Positions 187-325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000305603.

Chemistry

ChEMBLi CHEMBL3269.

Protein family/group databases

CAZyi GT10. Glycosyltransferase Family 10.

PTM databases

PhosphoSitei P21217.

Polymorphism databases

DMDMi 121137.

Proteomic databases

PaxDbi P21217.
PRIDEi P21217.

Protocols and materials databases

DNASUi 2525.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000303225 ; ENSP00000305603 ; ENSG00000171124 .
ENST00000458379 ; ENSP00000416443 ; ENSG00000171124 .
ENST00000589620 ; ENSP00000465804 ; ENSG00000171124 .
ENST00000589918 ; ENSP00000468123 ; ENSG00000171124 .
GeneIDi 2525.
KEGGi hsa:2525.
UCSCi uc002mdj.2. human.

Organism-specific databases

CTDi 2525.
GeneCardsi GC19M005843.
HGNCi HGNC:4014. FUT3.
HPAi HPA046966.
MIMi 111100. gene+phenotype.
neXtProti NX_P21217.
PharmGKBi PA28430.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG283180.
GeneTreei ENSGT00680000099679.
HOGENOMi HOG000045583.
HOVERGENi HBG000274.
InParanoidi P21217.
KOi K00716.
OrthoDBi EOG7RJPS6.
PhylomeDBi P21217.
TreeFami TF316348.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.1.65. 2681.

Miscellaneous databases

GeneWikii Fucosyltransferase_3.
GenomeRNAii 2525.
NextBioi 9943.
PROi P21217.
SOURCEi Search...

Gene expression databases

Bgeei P21217.
CleanExi HS_FUT3.
ExpressionAtlasi P21217. baseline and differential.
Genevestigatori P21217.

Family and domain databases

InterProi IPR001503. Glyco_trans_10.
[Graphical view ]
PANTHERi PTHR11929. PTHR11929. 1 hit.
Pfami PF00852. Glyco_transf_10. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase."
    Kukowska-Latallo J.F., Larsen R.D., Nair R.P., Lowe J.B.
    Genes Dev. 4:1288-1303(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression of human chromosome 19p alpha(1,3)-fucosyltransferase genes in normal tissues. Alternative splicing, polyadenylation, and isoforms."
    Cameron H.S., Szczepaniak D., Weston B.W.
    J. Biol. Chem. 270:20112-20122(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Isolation and expression of human alpha (1,3/1,4) fucosyltransferase."
    Rahim I., Schmidt L.R., Wahl D., Drayson E., Maslanik W., Stranahan P.L., Pettijohn D.E.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Squamous cell carcinoma.
  4. "Allele frequencies of fucosyltransferases 1, 2, and 3 of Styrian blood donors."
    Matzhold E.M.
    Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-68 AND MET-105.
    Tissue: Blood.
  5. SeattleSNPs variation discovery resource
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-5; ARG-20; ARG-68; MET-105; CYS-160; ASN-162; SER-170; ARG-223; MET-270; MET-325 AND GLN-327.
  6. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS ARG-68 AND MET-105.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon and Lung.
  8. "Genotypic heterogeneity among Lewis negative individuals."
    Elmgren A., Rydberg L., Larson G.
    Biochem. Biophys. Res. Commun. 196:515-520(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT LE(-) MET-105.
  9. "Alpha (1,3/1,4)fucosyltransferase (FucT-III) gene is inactivated by a single amino acid substitution in Lewis histo-blood type negative individuals."
    Nishihara S., Yazawa S., Iwasaki H., Nakazato M., Kudo T., Ando T., Narimatsu H.
    Biochem. Biophys. Res. Commun. 196:624-631(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS LE(-) ARG-20; SER-170 AND ALA-336.
  10. "Analysis of Lewis fucosyltransferase genes from the human gastric mucosa of Lewis-positive and -negative individuals."
    Koda Y., Kimura H., Mekada E.
    Blood 82:2915-2919(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS LE(-) ARG-20 AND SER-170.
  11. "Molecular basis for Lewis alpha(1,3/1,4)-fucosyltransferase gene deficiency (FUT3) found in Lewis-negative Indonesian pedigrees."
    Mollicone R., Reguigne I., Kelly R.J., Fletcher A., Watt J., Chatfield S., Aziz A., Cameron H.S., Weston B.W., Lowe J.B., Oriol R.
    J. Biol. Chem. 269:20987-20994(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS LE(-) ARG-20 AND LYS-356.
  12. "Molecular genetic analysis of the human Lewis histo-blood group system."
    Nishihara S., Narimatsu H., Iwasaki H., Yazawa S., Akamatsu S., Ando T., Seno T., Narimatsu I.
    J. Biol. Chem. 269:29271-29278(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT LE(-) LYS-356.
  13. "DNA sequencing and screening for point mutations in the human Lewis 'FUT3' gene enables molecular genotyping of the human Lewis blood group system."
    Elmgren A., Boerjeson C., Svensson L., Rydberg L., Larson G.
    Vox Sang. 70:97-103(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS LE(-) ARG-20; ARG-68; MET-105 AND LYS-356.
  14. "Significance of individual point mutations, T202C and C314T, in the human Lewis 'FUT3' gene for expression of Lewis antigens by the human alpha'1,3/1,4'-fucosyltransferase, Fuc-TIII."
    Elmgren A., Mollicone R., Costache M., Boerjeson C., Oriol R., Harrington J., Larson G.
    J. Biol. Chem. 272:21994-21998(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS LE(-) ARG-68 AND MET-105.
  15. "Five novel missense mutations of the Lewis gene 'FUT3' in African 'Xhosa' and Caucasian populations in South Africa."
    Pang H., Liu Y., Koda Y., Soejima M., Jia J., Schlaphoff T., du Toit E.D., Kimura H.
    Hum. Genet. 102:675-680(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS LE(+) LYS-102 AND ALA-124, VARIANTS LE(-) ASN-162; ARG-223 AND MET-270.

Entry informationi

Entry nameiFUT3_HUMAN
AccessioniPrimary (citable) accession number: P21217
Secondary accession number(s): B5U7U9
, B5U7V0, Q32NE7, Q99448, Q99449
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 29, 2014
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Blood group antigen proteins
    Nomenclature of blood group antigens and list of entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3