Reviewed, UniProtKB/Swiss-Prot P21217 (FUT3_HUMAN)

Names and origin

Protein names

Recommended name:
    Galactoside 3(4)-L-fucosyltransferase
    EC=2.4.1.65
Alternative name(s):
    Blood group Lewis alpha-4-fucosyltransferase
      Short name=Lewis FT
    Fucosyltransferase 3
    Fucosyltransferase III
      Short name=FucT-III

Gene names

Name:	FUT3
Synonyms:	FT3B, LE

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme.
Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->4))-N-acetyl-beta-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Tissue specificity	Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.
Miscellaneous	Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Sequence similarities	Belongs to the glycosyltransferase 10 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Molecular function	Blood group antigen Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Galactoside 3(4)-L-fucosyltransferase		PRO_0000221096
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 34	19	Signal-anchor for type II membrane protein Potential
	Topological domain	35 – 361	327	Lumenal Potential
Amino acid modifications
	Glycosylation	154	1	N-linked (GlcNAc...) Probable
	Glycosylation	185	1	N-linked (GlcNAc...) Probable
Natural variations
	Natural variant	5	1	G → S: dbSNP rs28362458. Ref.5		VAR_022200
	Natural variant	20	1	L → R in Le(-). dbSNP rs28362459. Ref.5 Ref.9 Ref.10 Ref.11 Ref.13		VAR_003426
	Natural variant	68	1	W → R in Le(-). dbSNP rs812936. Ref.5 Ref.13 Ref.4 Ref.6 Ref.14		VAR_007959
	Natural variant	102	1	Q → K in Le(+). Ref.15		VAR_007960
	Natural variant	105	1	T → M in Le(-). dbSNP rs778986. Ref.5 Ref.13 Ref.6 Ref.14 Ref.8		VAR_003427
	Natural variant	124	1	S → A in Le(+). Ref.15		VAR_007961
	Natural variant	160	1	R → C: dbSNP rs28362462. Ref.5		VAR_022201
	Natural variant	162	1	D → N in Le(-). dbSNP rs28362463. Ref.5 Ref.15		VAR_007962
	Natural variant	170	1	G → S in Le(-); completely inactive. dbSNP rs28362464. Ref.5 Ref.9 Ref.10		VAR_003428
	Natural variant	223	1	G → R in Le(-). dbSNP rs28362466. Ref.5 Ref.15		VAR_007963
	Natural variant	270	1	V → M in Le(-). dbSNP rs28381968. Ref.5 Ref.15		VAR_007964
	Natural variant	325	1	T → M: dbSNP rs28381969. Ref.5		VAR_022202
	Natural variant	327	1	R → Q: dbSNP rs28381970. Ref.5		VAR_022203
	Natural variant	336	1	D → A in Le(-). Ref.9		VAR_003429
	Natural variant	356	1	I → K in Le(-); less than 10% reduction in activity. dbSNP rs3894326. Ref.11 Ref.13 Ref.12		VAR_003430

Sequences

	Sequence		Length	Mass (Da)
	P21217-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: BF4398044F19C284	FASTA	361	42,117
	10 20 30 40 50 60 MDPLGAAKPQ WPWRRCLAAL LFQLLVAVCF FSYLRVSRDD ATGSPRAPSG SSRQDTTPTR 70 80 90 100 110 120 PTLLILLWTW PFHIPVALSR CSEMVPGTAD CHITADRKVY PQADTVIVHH WDIMSNPKSR 130 140 150 160 170 180 LPPSPRPQGQ RWIWFNLEPP PNCQHLEALD RYFNLTMSYR SDSDIFTPYG WLEPWSGQPA 190 200 210 220 230 240 HPPLNLSAKT ELVAWAVSNW KPDSARVRYY QSLQAHLKVD VYGRSHKPLP KGTMMETLSR 250 260 270 280 290 300 YKFYLAFENS LHPDYITEKL WRNALEAWAV PVVLGPSRSN YERFLPPDAF IHVDDFQSPK 310 320 330 340 350 360 DLARYLQELD KDHARYLSYF RWRETLRPRS FSWALDFCKA CWKLQQESRY QTVRSIAAWF T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase." Kukowska-Latallo J.F., Larsen R.D., Nair R.P., Lowe J.B. Genes Dev. 4:1288-1303(1990) [PubMed: 1977660] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression of human chromosome 19p alpha(1,3)-fucosyltransferase genes in normal tissues. Alternative splicing, polyadenylation, and isoforms." Cameron H.S., Szczepaniak D., Weston B.W. J. Biol. Chem. 270:20112-20122(1995) [PubMed: 7650030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Isolation and expression of human alpha (1,3/1,4) fucosyltransferase." Rahim I., Schmidt L.R., Wahl D., Drayson E., Maslanik W., Stranahan P.L., Pettijohn D.E. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Squamous cell carcinoma.
[4]	"Allele frequencies of fucosyltransferases 1, 2, and 3 of Styrian blood donors." Matzhold E.M. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-68. Tissue: Blood.
[5]	SeattleSNPs variation discovery resource Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-5; ARG-20; ARG-68; MET-105; CYS-160; ASN-162; SER-170; ARG-223; MET-270; MET-325 AND GLN-327.
[6]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS ARG-68 AND MET-105.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Lung.
[8]	"Genotypic heterogeneity among Lewis negative individuals." Elmgren A., Rydberg L., Larson G. Biochem. Biophys. Res. Commun. 196:515-520(1993) [PubMed: 8240322] [Abstract] Cited for: VARIANT LE(-) MET-105.
[9]	"Alpha (1,3/1,4)fucosyltransferase (FucT-III) gene is inactivated by a single amino acid substitution in Lewis histo-blood type negative individuals." Nishihara S., Yazawa S., Iwasaki H., Nakazato M., Kudo T., Ando T., Narimatsu H. Biochem. Biophys. Res. Commun. 196:624-631(1993) [PubMed: 8240337] [Abstract] Cited for: VARIANTS LE(-) ARG-20; SER-170 AND ALA-336.
[10]	"Analysis of Lewis fucosyltransferase genes from the human gastric mucosa of Lewis-positive and -negative individuals." Koda Y., Kimura H., Mekada E. Blood 82:2915-2919(1993) [PubMed: 8219240] [Abstract] Cited for: VARIANTS LE(-) ARG-20 AND SER-170.
[11]	"Molecular basis for Lewis alpha(1,3/1,4)-fucosyltransferase gene deficiency (FUT3) found in Lewis-negative Indonesian pedigrees." Mollicone R., Reguigne I., Kelly R.J., Fletcher A., Watt J., Chatfield S., Aziz A., Cameron H.S., Weston B.W., Lowe J.B., Oriol R. J. Biol. Chem. 269:20987-20994(1994) [PubMed: 8063716] [Abstract] Cited for: VARIANTS LE(-) ARG-20 AND LYS-356.
[12]	"Molecular genetic analysis of the human Lewis histo-blood group system." Nishihara S., Narimatsu H., Iwasaki H., Yazawa S., Akamatsu S., Ando T., Seno T., Narimatsu I. J. Biol. Chem. 269:29271-29278(1994) [PubMed: 7961897] [Abstract] Cited for: VARIANT LE(-) LYS-356.
[13]	"DNA sequencing and screening for point mutations in the human Lewis 'FUT3' gene enables molecular genotyping of the human Lewis blood group system." Elmgren A., Boerjeson C., Svensson L., Rydberg L., Larson G. Vox Sang. 70:97-103(1996) [PubMed: 8801770] [Abstract] Cited for: VARIANTS LE(-) ARG-20; ARG-68; MET-105 AND LYS-356.
[14]	"Significance of individual point mutations, T202C and C314T, in the human Lewis 'FUT3' gene for expression of Lewis antigens by the human alpha'1,3/1,4'-fucosyltransferase, Fuc-TIII." Elmgren A., Mollicone R., Costache M., Boerjeson C., Oriol R., Harrington J., Larson G. J. Biol. Chem. 272:21994-21998(1997) [PubMed: 9268337] [Abstract] Cited for: VARIANTS LE(-) ARG-68 AND MET-105.
[15]	"Five novel missense mutations of the Lewis gene 'FUT3' in African 'Xhosa' and Caucasian populations in South Africa." Pang H., Liu Y., Koda Y., Soejima M., Jia J., Schlaphoff T., du Toit E.D., Kimura H. Hum. Genet. 102:675-680(1998) [PubMed: 9703429] [Abstract] Cited for: VARIANTS LE(+) LYS-102 AND ALA-124, VARIANTS LE(-) ASN-162; ARG-223 AND MET-270.
+	Additional computationally mapped references.

Web resources

dbRBC/BGMUT

Blood group antigen gene mutation database

GGDB

GlycoGene database

SeattleSNPs

Functional Glycomics Gateway - GTase

Fucosyltransferase 3

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53578 mRNA. Translation: CAA37641.1. U27326 mRNA. Translation: AAC50185.1. U27327 mRNA. Translation: AAC50186.1. U27328 mRNA. Translation: AAC50187.1. D89324 Genomic DNA. Translation: BAA13941.1. D89325 Genomic DNA. Translation: BAA13942.1. AF131913 mRNA. Translation: AAD33514.1. FM210024 Genomic DNA. Translation: CAR64692.1. AY870341 Genomic DNA. Translation: AAW34365.1. AC024592 Genomic DNA. No translation available. BC074836 mRNA. Translation: AAH74836.1. BC074837 mRNA. Translation: AAH74837.1. BC108675 mRNA. Translation: AAI08676.1.
IPI	IPI00007671.
PIR	A36669.
RefSeq	NP_000140.1. NP_001091108.1. NP_001091109.1. NP_001091110.1.
UniGene	Hs.169238 Hs.631846
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P21217.
Protein family/group databases
CAZy	GT10. Glycosyltransferase Family 10.
Proteomic databases
PRIDE	P21217.
Genome annotation databases
Ensembl	ENST00000303225; ENSP00000305603; ENSG00000171124; Homo sapiens. [Genome view] ENST00000402214; ENSP00000384749; ENSG00000171124; Homo sapiens. [Genome view] ENST00000443606; ENSP00000408589; ENSG00000171124; Homo sapiens. [Genome view] ENST00000458379; ENSP00000416443; ENSG00000171124; Homo sapiens. [Genome view]
GeneID	2525.
KEGG	hsa:2525.
UCSC	uc002mdj.2. human.
Organism-specific databases
CTD	2525.
GeneCards	GC19M005793.
H-InvDB	HIX0039971.
HGNC	HGNC:4014. FUT3.
MIM	111100. gene+phenotype.
PharmGKB	PA28430.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P21217.
HOVERGEN	P21217.
Enzyme and pathway databases
BRENDA	2.4.1.65. 247.
Gene expression databases
ArrayExpress	P21217.
Bgee	P21217.
CleanEx	HS_FUT3.
Genevestigator	P21217.
GermOnline	ENSG00000171124. Homo sapiens.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
PANTHER	PTHR11929. Glyco_trans_10. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	9943.
SOURCE	Search...

Entry information

Entry name

FUT3_HUMAN

Accession

Primary (citable) accession number: P21217
Secondary accession number(s): B5U7U9 , Q32NE7, Q99448, Q99449

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	October 13, 2009
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.