P21216 (IPYR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble inorganic pyrophosphatase 2
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase 2
Short name=PPase 2

Gene names

Name:	PPA2
Synonyms:	PPA
Ordered Locus Names:	At2g18230
ORF Names:	T30D6.26

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.
Sequence caution	The sequence CAA40764.1 differs from that shown. Reason: Frameshift at position 209.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Non-traceable author statement. Source: TAIR magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Soluble inorganic pyrophosphatase 2		PRO_0000137574
Sites
	Metal binding	104	1	Magnesium 1 By similarity
	Metal binding	109	1	Magnesium 1 By similarity
	Metal binding	109	1	Magnesium 2 By similarity
	Metal binding	141	1	Magnesium 1 By similarity
Experimental info
	Sequence conflict	12	1	G → A in AAM66110. Ref.5
	Sequence conflict	37	1	A → S in AAM66110. Ref.5
	Sequence conflict	58	1	V → A in CAA40764. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21216 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 884DCFAB6B1B239A	FASTA	218	24,671
	10 20 30 40 50 60 MAEIKDEGSA KGYAFPLRNP NVTLNERNFA AFTHRSAAAH PWHDLEIGPE APTVFNCVVE 70 80 90 100 110 120 ISKGGKVKYE LDKNSGLIKV DRVLYSSIVY PHNYGFIPRT ICEDSDPMDV LVLMQEPVLT 130 140 150 160 170 180 GSFLRARAIG LMPMIDQGEK DDKIIAVCAD DPEFRHYRDI KELPPHRLAE IRRFFEDYKK 190 200 210 NENKKVDVEA FLPAQAAIDA IKDSMDLYAA YIKAGLQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of an inorganic pyrophosphatase gene from Arabidopsis thaliana." Kieber J.J., Signer E.R. Plant Mol. Biol. 16:345-348(1991) [PubMed: 1654155] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57545 mRNA. Translation: CAA40764.1. Frameshift. AC006439 Genomic DNA. Translation: AAD15513.1. CP002685 Genomic DNA. Translation: AEC06742.1. AY048209 mRNA. Translation: AAK82472.1. AY091698 mRNA. Translation: AAM10297.1. AY088579 mRNA. Translation: AAM66110.1.
IPI	IPI00542975.
PIR	H84561. S13379.
RefSeq	NP_179415.1. NM_127380.3.
UniGene	At.22749.
3D structure databases
ProteinModelPortal	P21216.
SMR	P21216. Positions 12-216.
ModBase	Search...
Protein-protein interaction databases
STRING	P21216.
Proteomic databases
PRIDE	P21216.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G18230.1; AT2G18230.1; AT2G18230.
GeneID	816338.
GenomeReviews	Gene locus AT2G18230 in contig CT485783_GR.
KEGG	ath:AT2G18230.
NMPDR	fig|3702.1.peg.8818.
Organism-specific databases
TAIR	At2g18230.
Phylogenomic databases
eggNOG	KOG1626.
GeneTree	EPGT00050000000788.
HOGENOM	HBG529150.
InParanoid	P21216.
OMA	DRILYGD.
PhylomeDB	P21216.
ProtClustDB	PLN02373.
Gene expression databases
ArrayExpress	P21216.
Genevestigator	P21216.
GermOnline	AT2G18230. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR2_ARATH

Accession

Primary (citable) accession number: P21216
Secondary accession number(s): Q8L988, Q9ZPV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 6, 2002
Last modified:	November 16, 2011
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program