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Protein

12-alpha-hydroxysteroid dehydrogenase

Gene
N/A
Organism
Clostridium sp. (strain ATCC 29733 / VPI C48-50)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of the 12-alpha-hydroxyl group of bile acids, both in their free and conjugated form. Also acts on bile alcohols.

Miscellaneous

The thermostability of the enzyme is greatly increased due to NADP binding.

Catalytic activityi

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NADP+ = 3-alpha,7-alpha-dihydroxy-12-oxo-5-beta-cholanate + NADPH.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processBile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15705

Names & Taxonomyi

Protein namesi
Recommended name:
12-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.176)
OrganismiClostridium sp. (strain ATCC 29733 / VPI C48-50)
Taxonomic identifieri1507 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000643471 – ›2912-alpha-hydroxysteroid dehydrogenaseAdd BLAST›29

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

SMRiP21215
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Fragment.

P21215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20 
MIFDGKVAII TGGGKAKSIG YGIAVAYAK
Length:29
Mass (Da):2,900
Last modified:August 1, 1991 - v1
Checksum:iA827DB34DB6C8812
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei291

Sequence databases

PIRiS14099

Similar proteinsi

Entry informationi

Entry namei12AH_CLOS4
AccessioniPrimary (citable) accession number: P21215
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: February 28, 2018
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
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Main funding by: National Institutes of Health