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Protein

Histidine ammonia-lyase

Gene

Hal

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-histidine = urocanate + NH3.PROSITE-ProRule annotation1 Publication

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 1 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (Hal)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

GO - Molecular functioni

  • histidine ammonia-lyase activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Histidine metabolism

Enzyme and pathway databases

ReactomeiR-RNO-70921. Histidine catabolism.
UniPathwayiUPA00379; UER00549.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine ammonia-lyase (EC:4.3.1.3)
Short name:
Histidase
Gene namesi
Name:Hal
Synonyms:Huth
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi68363. Hal.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi223 – 2231S → A: 2.4% of activity.
Mutagenesisi254 – 2541S → A: Complete loss of activity. 1 Publication
Mutagenesisi508 – 5081S → A: 75% of activity.
Mutagenesisi533 – 5331S → A: 16% of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Histidine ammonia-lyasePRO_0000161060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki253 ↔ 2555-imidazolinone (Ala-Gly)By similarity
Modified residuei254 – 25412,3-didehydroalanine (Ser)PROSITE-ProRule annotation
Modified residuei396 – 3961PhosphothreonineBy similarity
Modified residuei635 – 6351PhosphoserineCombined sources
Modified residuei637 – 6371PhosphothreonineBy similarity
Modified residuei648 – 6481PhosphoserineCombined sources

Post-translational modificationi

Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21213.
PRIDEiP21213.

PTM databases

iPTMnetiP21213.
PhosphoSiteiP21213.

Expressioni

Tissue specificityi

Liver and skin.

Gene expression databases

GenevisibleiP21213. RN.

Interactioni

Protein-protein interaction databases

DIPiDIP-37290N.
IntActiP21213. 1 interaction.
STRINGi10116.ENSRNOP00000006971.

Structurei

3D structure databases

ProteinModelPortaliP21213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAL/histidase family.Curated

Phylogenomic databases

eggNOGiKOG0222. Eukaryota.
COG2986. LUCA.
GeneTreeiENSGT00390000009047.
HOGENOMiHOG000237619.
HOVERGENiHBG004509.
InParanoidiP21213.
KOiK01745.
OMAiCFLVRRC.
OrthoDBiEOG73RB9V.
PhylomeDBiP21213.
TreeFamiTF313824.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR001106. Aromatic_Lyase.
IPR021922. DUF3534.
IPR024083. Fumarase/histidase_N.
IPR005921. HutH.
IPR008948. L-Aspartase-like.
IPR022313. Phe/His_NH3-lyase_AS.
[Graphical view]
PfamiPF12053. DUF3534. 1 hit.
PF00221. Lyase_aromatic. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR01225. hutH. 1 hit.
PROSITEiPS00488. PAL_HISTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRYTVHVRG EWLAVPCQDG KLSVGWLGRE AVRRYMKNKP DNGGFTSVDE
60 70 80 90 100
VRFLVRRCKG LGLLDNEDLL EVALEDNEFV EVVIEGDVMS PDFIPSQPEG
110 120 130 140 150
VFLYSKYREP EKYIALDGDS LSTEDLVNLG KGHYKIKLTS IAEKKVQQSR
160 170 180 190 200
EVIDSIIKER TVVYGITTGF GKFARTVIPA NKLQELQVNL VRSHSSGVGK
210 220 230 240 250
PLSPERCRML LALRINVLAK GYSGISLETL KQVIEVFNAS CLSYVPEKGT
260 270 280 290 300
VGASGDLAPL SHLALGLIGE GKMWSPKSGW ADAKYVLEAH GLKPIVLKPK
310 320 330 340 350
EGLALINGTQ MITSLGCEAV ERASAIARQA DIVAALTLEV LKGTTKAFDT
360 370 380 390 400
DIHAVRPHRG QIEVAFRFRS LLDSDHHPSE IAESHRFCDR VQDAYTLRCC
410 420 430 440 450
PQVHGVVNDT IAFVKDIITT ELNSATDNPM VFASRGETIS GGNFHGEYPA
460 470 480 490 500
KALDYLAIGV HELAAISERR IERLCNPSLS ELPAFLVAEG GLNSGFMIAH
510 520 530 540 550
CTAAALVSES KALCHPSSVD SLSTSAATED HVSMGGWAAR KALRVIEHVE
560 570 580 590 600
QVLAIELLAA CQGIEFLRPL KTTTPLEKVY DLVRSVVRPW IKDRFMAPDI
610 620 630 640 650
EAAHRLLLDQ KVWEVAAPYI EKYRMEHIPE SRPLSPTAFS LESLRKNSAT

IPESDDL
Length:657
Mass (Da):72,284
Last modified:May 1, 1991 - v1
Checksum:iCBED79FC6CD40FA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58308 mRNA. Translation: AAA63491.1.
BC089809 mRNA. Translation: AAH89809.1.
PIRiA36087.
RefSeqiNP_058855.1. NM_017159.1.
UniGeneiRn.10037.

Genome annotation databases

EnsembliENSRNOT00000006971; ENSRNOP00000006971; ENSRNOG00000004502.
GeneIDi29301.
KEGGirno:29301.
UCSCiRGD:68363. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58308 mRNA. Translation: AAA63491.1.
BC089809 mRNA. Translation: AAH89809.1.
PIRiA36087.
RefSeqiNP_058855.1. NM_017159.1.
UniGeneiRn.10037.

3D structure databases

ProteinModelPortaliP21213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-37290N.
IntActiP21213. 1 interaction.
STRINGi10116.ENSRNOP00000006971.

PTM databases

iPTMnetiP21213.
PhosphoSiteiP21213.

Proteomic databases

PaxDbiP21213.
PRIDEiP21213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000006971; ENSRNOP00000006971; ENSRNOG00000004502.
GeneIDi29301.
KEGGirno:29301.
UCSCiRGD:68363. rat.

Organism-specific databases

CTDi3034.
RGDi68363. Hal.

Phylogenomic databases

eggNOGiKOG0222. Eukaryota.
COG2986. LUCA.
GeneTreeiENSGT00390000009047.
HOGENOMiHOG000237619.
HOVERGENiHBG004509.
InParanoidiP21213.
KOiK01745.
OMAiCFLVRRC.
OrthoDBiEOG73RB9V.
PhylomeDBiP21213.
TreeFamiTF313824.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00549.
ReactomeiR-RNO-70921. Histidine catabolism.

Miscellaneous databases

NextBioi608720.
PROiP21213.

Gene expression databases

GenevisibleiP21213. RN.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR001106. Aromatic_Lyase.
IPR021922. DUF3534.
IPR024083. Fumarase/histidase_N.
IPR005921. HutH.
IPR008948. L-Aspartase-like.
IPR022313. Phe/His_NH3-lyase_AS.
[Graphical view]
PfamiPF12053. DUF3534. 1 hit.
PF00221. Lyase_aromatic. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR01225. hutH. 1 hit.
PROSITEiPS00488. PAL_HISTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of rat histidase. Homology to two bacterial histidases and four phenylalanine ammonia-lyases."
    Taylor R.G., Lambert M.A., Sexsmith E., Sadler S.J., Ray P.N., Mahuran D.J., McInnes R.R.
    J. Biol. Chem. 265:18192-18199(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  3. "Site-directed mutagenesis of conserved serines in rat histidase. Identification of serine 254 as an essential active site residue."
    Taylor R.G., McInnes R.R.
    J. Biol. Chem. 269:27473-27477(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, ENZYME MECHANISM, MUTAGENESIS OF SER-254.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635 AND SER-648, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHUTH_RAT
AccessioniPrimary (citable) accession number: P21213
Secondary accession number(s): Q5EBB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: January 20, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.