Reviewed, UniProtKB/Swiss-Prot P21203 (PHEA_BACSU)

Names and origin

Protein names

Recommended name:
    Prephenate dehydratase
      Short name=PDT
    EC=4.2.1.51

Gene names

Name:	pheA
Ordered Locus Names:	BSU27900

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Catalytic activity	Prephenate = phenylpyruvate + H2O + CO2.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.
Sequence similarities	Contains 1 ACT domain. Contains 1 prephenate dehydratase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Phenylalanine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro prephenate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Prephenate dehydratase		PRO_0000119176
Regions
	Domain	2 – 183	182	Prephenate dehydratase
	Domain	202 – 277	76	ACT
Sites
	Site	176	1	Essential for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21203-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: A480D3F931906EEF	FASTA	285	31,901
	10 20 30 40 50 60 MKVGYLGPAA TFTHLAVSSC FQNGAEHVAY RTIPECIDAA VAGEVDFAFV PLENALEGSV 70 80 90 100 110 120 NLTIDYLIHE QPLPIVGEMT LPIHQHLLVH PSRENAWKEL DKIYSHSHAI AQCHKFLHRH 130 140 150 160 170 180 FPSVPYEYAN STGAAAKFVS DHPELNIGVI ANDMAASTYE LKIVKRDIQD YRDNHTRFVI 190 200 210 220 230 240 LSPDENISFE VNSKLSSRPK TTLMVMLPQD DQSGALHRVL SAFSWRNLNL SKIESRPTKT 250 260 270 280 GLGHYFFIID IEKAFDDVLI PGAMQELEAL GCKVRLLGAY QSYQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein." Trach K., Hoch J.A. J. Bacteriol. 171:1362-1371(1989) [PubMed: 2537815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis." Sun D., Setlow P.L. J. Bacteriol. 175:1423-1432(1993) [PubMed: 8444804] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 282-285. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24537 Genomic DNA. Translation: AAA22507.1. AL009126 Genomic DNA. Translation: CAB14750.1. M98822 Genomic DNA. No translation available.
PIR	C32804.
RefSeq	NP_390668.1.
3D structure databases
SMR	P21203. Positions 1-281.
ModBase	Search...
Genome annotation databases
GeneID	937513.
GenomeReviews	Gene locus BSU27900 in contig AL009126_GR.
KEGG	bsu:BSU27900.
NMPDR	fig|224308.1.peg.2793.
Organism-specific databases
SubtiList	BG10914. pheA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG693866.
OMA	GLGNYFF.
PhylomeDB	P21203.
Enzyme and pathway databases
BRENDA	4.2.1.51. 150.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001086. Preph_deHydtase. IPR018528. Preph_deHydtase_CS. [Graphical view]
Pfam	PF01842. ACT. 1 hit. PF00800. PDT. 1 hit. [Graphical view]
PROSITE	PS00857. PREPHENATE_DEHYDR_1. 1 hit. PS00858. PREPHENATE_DEHYDR_2. 1 hit. PS51171. PREPHENATE_DEHYDR_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHEA_BACSU

Accession

Primary (citable) accession number: P21203

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	January 19, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)