Metallothionein expression activator - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P21192 (ACE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 121. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Metallothionein expression activator

Gene names

Name:	ACE2
Ordered Locus Names:	YLR131C
ORF Names:	L3123, L9606.10

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	770 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in regulating basal-level expression of CUP1. Activates EGT2 transcription in the absence of SWI5.
Subcellular location	Nucleus.
Miscellaneous	Present with 538 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of cytokinetic cell separation involved in cell cycle cytokinesis Inferred from mutant phenotype. Source: SGD regulation of transcription involved in G1 phase of mitotic cell cycle Inferred from mutant phenotype. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype. Source: SGD core promoter proximal region sequence-specific DNA binding Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 770

770

Metallothionein expression activator

PRO_0000046800

Regions

Zinc finger

603 – 627

C2H2-type 1

Zinc finger

633 – 657

C2H2-type 2

Zinc finger

662 – 685

C2H2-type 3; atypical

Amino acid modifications

Modified residue

Phosphoserine Ref.6 Ref.7

Modified residue

122

Phosphoserine Ref.6

Modified residue

249

Phosphoserine Ref.10

Modified residue

253

Phosphoserine Ref.10

Modified residue

428

Phosphoserine Ref.8

Modified residue

501

Phosphothreonine Ref.8

Modified residue

564

Phosphoserine Ref.10

Modified residue

701

Phosphoserine Ref.4

Modified residue

709

Phosphoserine Ref.9 Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P21192 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: A7FCA8C506A2FD75
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FASTA

770

86,634

        10         20         30         40         50         60 
MDNVVDPWYI NPSGFAKDTQ DEEYVQHHDN VNPTIPPPDN YILNNENDDG LDNLLGMDYY 

        70         80         90        100        110        120 
NIDDLLTQEL RDLDIPLVPS PKTGDGSSDK KNIDRTWNLG DENNKVSHYS KKSMSSHKRG 

       130        140        150        160        170        180 
LSGTAIFGFL GHNKTLSISS LQQSILNMSK DPQPMELINE LGNHNTVKNN NDDFDHIREN 

       190        200        210        220        230        240 
DGENSYLSQV LLKQQEELRI ALEKQKEVNE KLEKQLRDNQ IQQEKLRKVL EEQEEVAQKL 

       250        260        270        280        290        300 
VSGATNSNSK PGSPVILKTP AMQNGRMKDN AIIVTTNSAN GGYQFPPPTL ISPRMSNTSI 

       310        320        330        340        350        360 
NGSPSRKYHR QRYPNKSPES NGLNLFSSNS GYLRDSELLS FSPQNYNLNL DGLTYNDHNN 

       370        380        390        400        410        420 
TSDKNNNDKK NSTGDNIFRL FEKTSPGGLS ISPRINGNSL RSPFLVGTDK SRDDRYAAGT 

       430        440        450        460        470        480 
FTPRTQLSPI HKKRESVVST VSTISQLQDD TEPIHMRNTQ NPTLRNANAL ASSSVLPPIP 

       490        500        510        520        530        540 
GSSNNTPIKN SLPQKHVFQH TPVKAPPKNG SNLAPLLNAP DLTDHQLEIK TPIRNNSHCE 

       550        560        570        580        590        600 
VESYPQVPPV THDIHKSPTL HSTSPLPDEI IPRTTPMKIT KKPTTLPPGT IDQYVKELPD 

       610        620        630        640        650        660 
KLFECLYPNC NKVFKRRYNI RSHIQTHLQD RPYSCDFPGC TKAFVRNHDL IRHKISHNAK 

       670        680        690        700        710        720 
KYICPCGKRF NREDALMVHR SRMICTGGKK LEHSINKKLT SPKKSLLDSP HDTSPVKETI 

       730        740        750        760        770 
ARDKDGSVLM KMEEQLRDDM RKHGLLDPPP STAAHEQNSN RTLSNETDAL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ACE2, an activator of yeast metallothionein expression which is homologous to SWI5." Butler G., Thiele D.J. Mol. Cell. Biol. 11:476-485(1991) [PubMed: 1986241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DBY939.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-701, MASS SPECTROMETRY. Strain: 2124.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-122, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428 AND THR-501, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-709, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-249; SER-253; SER-564 AND SER-709, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55619 Genomic DNA. Translation: AAA34387.1. X91258 Genomic DNA. Translation: CAA62643.1. Z73303 Genomic DNA. Translation: CAA97702.1. U53881 Genomic DNA. Translation: AAB82398.1. BK006945 Genomic DNA. Translation: DAA09442.1.
PIR	TWBYA2. S12943.
RefSeq	NP_013232.1. NM_001182018.1.
3D structure databases
ProteinModelPortal	P21192.
SMR	P21192. Positions 585-767.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2012N.
IntAct	P21192. 8 interactions.
MINT	MINT-388183.
STRING	P21192.
Proteomic databases
PeptideAtlas	P21192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR131C; YLR131C; YLR131C.
GeneID	850822.
KEGG	sce:YLR131C.
NMPDR	fig\|4932.3.peg.4225.
Organism-specific databases
CYGD	YLR131c.
SGD	S000004121. ACE2.
Phylogenomic databases
eggNOG	fuNOG11506.
GeneTree	EFGT00050000000791.
HOGENOM	HBG202461.
OMA	FQHTPVK.
OrthoDB	EOG49KK17.
Gene expression databases
ArrayExpress	P21192.
Genevestigator	P21192.
GermOnline	YLR131C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.
KO	K09238.
Pfam	PF00096. zf-C2H2. 2 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	967073.

Entry information

Entry name

ACE2_YEAST

Accession

Primary (citable) accession number: P21192
Secondary accession number(s): D6VYC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	December 14, 2011
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents