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P21189 (DPO2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase II
Short name=Pol II
EC=2.7.7.7
Gene names
Name:polB
Synonyms:dinA
Ordered Locus Names:b0060, JW0059
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length783 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thought to be involved in DNA repair and/or mutagenesis.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Enzyme regulation

DNA polymerase II activity is regulated by the lexA gene during the SOS response.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.6
Chain2 – 783782DNA polymerase II
PRO_0000046473

Natural variations

Natural variant4011G → D in allele POLB100.

Experimental info

Sequence conflict1721G → A in AAA24406. Ref.2
Sequence conflict1721G → A in AAA24407. Ref.2
Sequence conflict257 – 2582EH → DD in AAA63764. Ref.6
Sequence conflict257 – 2582EH → DD in AAA23684. Ref.6
Sequence conflict2721R → G in AAA63764. Ref.6
Sequence conflict2721R → G in AAA23684. Ref.6
Sequence conflict7351N → T in AAA24406. Ref.2
Sequence conflict7351N → T in AAA24407. Ref.2
Sequence conflict740 – 78344LDYQR…QLGLF → PGLPTFTTGLRTLSDPPATT RGGGNTPFY Ref.2

Secondary structure

1 ............................................................................................................................ 783
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P21189 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 1A12A21B2FDB0E22

FASTA78390,053
        10         20         30         40         50         60 
MAQAGFILTR HWRDTPQGTE VSFWLATDNG PLQVTLAPQE SVAFIPADQV PRAQHILQGE 

        70         80         90        100        110        120 
QGFRLTPLAL KDFHRQPVYG LYCRAHRQLM NYEKRLREGG VTVYEADVRP PERYLMERFI 

       130        140        150        160        170        180 
TSPVWVEGDM HNGTIVNARL KPHPDYRPPL KWVSIDIETT RHGELYCIGL EGCGQRIVYM 

       190        200        210        220        230        240 
LGPENGDASS LDFELEYVAS RPQLLEKLNA WFANYDPDVI IGWNVVQFDL RMLQKHAERY 

       250        260        270        280        290        300 
RLPLRLGRDN SELEWREHGF KNGVFFAQAK GRLIIDGIEA LKSAFWNFSS FSLETVAQEL 

       310        320        330        340        350        360 
LGEGKSIDNP WDRMDEIDRR FAEDKPALAT YNLKDCELVT QIFHKTEIMP FLLERATVNG 

       370        380        390        400        410        420 
LPVDRHGGSV AAFGHLYFPR MHRAGYVAPN LGEVPPHASP GGYVMDSRPG LYDSVLVLDY 

       430        440        450        460        470        480 
KSLYPSIIRT FLIDPVGLVE GMAQPDPEHS TEGFLDAWFS REKHCLPEIV TNIWHGRDEA 

       490        500        510        520        530        540 
KRQGNKPLSQ ALKIIMNAFY GVLGTTACRF FDPRLASSIT MRGHQIMRQT KALIEAQGYD 

       550        560        570        580        590        600 
VIYGDTDSTF VWLKGAHSEE EAAKIGRALV QHVNAWWAET LQKQRLTSAL ELEYETHFCR 

       610        620        630        640        650        660 
FLMPTIRGAD TGSKKRYAGL IQEGDKQRMV FKGLETVRTD WTPLAQQFQQ ELYLRIFRNE 

       670        680        690        700        710        720 
PYQEYVRETI DKLMAGELDA RLVYRKRLRR PLSEYQRNVP PHVRAARLAD EENQKRGRPL 

       730        740        750        760        770        780 
QYQNRGTIKY VWTTNGPEPL DYQRSPLDYE HYLTRQLQPV AEGILPFIED NFATLMTGQL 


GLF

« Hide

References

« Hide 'large scale' references
[1]"Escherichia coli DNA polymerase II is homologous to alpha-like DNA polymerases."
Iwasaki H., Ishino Y., Toh H., Nakata A., Shinagawa H.
Mol. Gen. Genet. 226:24-33(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Nucleotide sequence and deletion analysis of the polB gene of Escherichia coli."
Chen H., Sun Y., Stark T., Beattie W., Moses R.E.
DNA Cell Biol. 9:631-635(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli."
Bonner C.A., Hays S., McEntee K., Goodman M.F.
Proc. Natl. Acad. Sci. U.S.A. 87:7663-7667(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-458, PROTEIN SEQUENCE OF 2-28.
Strain: K12.
[7]"The Escherichia coli polB locus is identical to dinA, the structural gene for DNA polymerase II. Characterization of Pol II purified from a polB mutant."
Qiu Z., Goodman M.F.
J. Biol. Chem. 272:8611-8617(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT MUTANT ALLELE POLB100.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X54847 Genomic DNA. Translation: CAA38616.1.
M62646 Genomic DNA. Translation: AAA24406.1.
M35371 Genomic DNA. Translation: AAA24407.1.
M38283 Genomic DNA. Translation: AAA63764.1.
U00096 Genomic DNA. Translation: AAC73171.1.
AP009048 Genomic DNA. Translation: BAB96628.2.
M37727 Genomic DNA. Translation: AAA23684.1.
PIRJDEC22. S15943.
RefSeqNP_414602.1. NC_000913.2.
YP_488366.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q8IX-ray2.00A1-783[»]
3K57X-ray2.08A1-783[»]
3K58X-ray2.05A1-783[»]
3K59X-ray1.92A1-783[»]
3K5LX-ray2.70A1-783[»]
3K5MX-ray2.04A1-783[»]
3K5NX-ray3.15A/B1-783[»]
3K5OX-ray2.20A/B1-783[»]
3MAQX-ray2.40A1-783[»]
ProteinModelPortalP21189.
SMRP21189. Positions 1-780.
ModBaseSearch...

Protein-protein interaction databases

IntActP21189. 4 interactions.
STRING511145.b0060.

Proteomic databases

PRIDEP21189.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73171; AAC73171; b0060.
BAB96628; BAB96628; BAB96628.
GeneID12932627.
944779.
KEGGecj:Y75_p0060.
eco:b0060.
PATRIC32115221. VBIEscCol129921_0062.

Organism-specific databases

EchoBASEEB0740.
EcoGeneEG10747. polB.

Phylogenomic databases

eggNOGCOG0417.
HOGENOMHOG000221829.
KOK02336.
OMAVLDGIDW.
ProtClustDBPRK05762.

Enzyme and pathway databases

BioCycEcoCyc:EG10747-MONOMER.
ECOL316407:JW0059-MONOMER.
MetaCyc:EG10747-MONOMER.

Gene expression databases

GenevestigatorP21189.

Family and domain databases

Gene3D3.90.1600.10. 2 hits.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 2 hits.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSPR00106. DNAPOLB.
SMARTSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. RNaseH_fold. 1 hit.
PROSITEPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP21189.

Entry information

Entry nameDPO2_ECOLI
AccessionPrimary (citable) accession number: P21189
Secondary accession number(s): Q8KMX7, Q8KMX8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents