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Protein

Complement C2

Gene

C2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.

Miscellaneous

C2 is a major histocompatibility complex class-III protein.

Catalytic activityi

Selective cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to form C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to form C5a and C5b.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi269Divalent metal cationBy similarity1
Metal bindingi271Divalent metal cationBy similarity1
Metal bindingi344Divalent metal cationBy similarity1
Active sitei514Charge relay systemBy similarity1
Active sitei570Charge relay systemBy similarity1
Active sitei689Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processComplement pathway, Immunity, Innate immunity
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-MMU-166663 Initial triggering of complement
R-MMU-174577 Activation of C3 and C5
R-MMU-977606 Regulation of Complement cascade

Protein family/group databases

MEROPSiS01.194

Names & Taxonomyi

Protein namesi
Recommended name:
Complement C2 (EC:3.4.21.43)
Alternative name(s):
C3/C5 convertase
Cleaved into the following 2 chains:
Gene namesi
Name:C2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:88226 C2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
ChainiPRO_000002761319 – 760Complement C2Add BLAST742
ChainiPRO_000002761419 – 250Complement C2b fragmentAdd BLAST232
ChainiPRO_0000027615251 – 760Complement C2a fragmentAdd BLAST510

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 62By similarity
Glycosylationi27N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi32N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi49 ↔ 89By similarity
Disulfide bondi94 ↔ 136By similarity
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi122 ↔ 149By similarity
Disulfide bondi156 ↔ 197By similarity
Disulfide bondi182 ↔ 210By similarity
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi340N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi474N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi478N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi499 ↔ 515By similarity
Glycosylationi663N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi685 ↔ 715By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP21180
PaxDbiP21180
PeptideAtlasiP21180
PRIDEiP21180

PTM databases

iPTMnetiP21180
PhosphoSitePlusiP21180

Expressioni

Gene expression databases

BgeeiENSMUSG00000024371
CleanExiMM_C2
ExpressionAtlasiP21180 baseline and differential
GenevisibleiP21180 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025230

Structurei

3D structure databases

ProteinModelPortaliP21180
SMRiP21180
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 90Sushi 1PROSITE-ProRule annotationAdd BLAST71
Domaini92 – 151Sushi 2PROSITE-ProRule annotationAdd BLAST60
Domaini154 – 212Sushi 3PROSITE-ProRule annotationAdd BLAST59
Domaini261 – 459VWFAPROSITE-ProRule annotationAdd BLAST199
Domaini471 – 752Peptidase S1PROSITE-ProRule annotationAdd BLAST282

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi267 – 271MIDAS-like motifBy similarity5

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations.By similarity

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00530000063826
HOGENOMiHOG000038034
HOVERGENiHBG002567
InParanoidiP21180
KOiK01332
OMAiRNVSEFY
OrthoDBiEOG091G02G8
TreeFamiTF330194

Family and domain databases

CDDicd00033 CCP, 2 hits
cd00190 Tryp_SPc, 1 hit
Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR011360 Compl_C2_B
IPR037568 Complement_C2
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PANTHERiPTHR19325:SF387 PTHR19325:SF387, 1 hit
PfamiView protein in Pfam
PF00084 Sushi, 2 hits
PF00089 Trypsin, 1 hit
PF00092 VWA, 1 hit
PIRSFiPIRSF001154 Compl_C2_B, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00032 CCP, 2 hits
SM00020 Tryp_SPc, 1 hit
SM00327 VWA, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF53300 SSF53300, 1 hit
SSF57535 SSF57535, 3 hits
PROSITEiView protein in PROSITE
PS50923 SUSHI, 3 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
PS50234 VWFA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P21180-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPLLALFYL LQLGPGLAAL FCNQNVNITG GNFTLSHGWA PGSLLIYSCP
60 70 80 90 100
LGRYPSPAWR KCQSNGQWLT PRSSSHHTLR SSRMVKAVCK PVRCLAPSSF
110 120 130 140 150
ENGIYFPRLV SYPVGSNVSF ECEQDFTLRG SPVRYCRPNG LWDGETAVCD
160 170 180 190 200
NGASHCPNPG ISVGTARTGL NFDLGDKVRY RCSSSNMVLT GSAERECQSN
210 220 230 240 250
GVWSGSEPIC RQPYSYDFPE DVASALDTSL TNLLGATNPT QNLLTKSLGR
260 270 280 290 300
KIIIQRSGHL NLYLLLDASQ SVTEKDFDIF KKSAELMVER IFSFEVNVSV
310 320 330 340 350
AIITFASQPK TIMSILSERS QDVTEVITSL DSASYKDHEN ATGTNTYEVL
360 370 380 390 400
IRVYSMMQSQ MDRLGMETSA WKEIRHTIIL LTDGKSNMGD SPKKAVTRIR
410 420 430 440 450
ELLSIEQNRD DYLDIYAIGV GKLDVDWKEL NELGSKKDGE RHAFILQDAK
460 470 480 490 500
ALQQIFEHML DVSKLTDTIC GVGNMSANAS DQERTPWQVT FKPKSKETCQ
510 520 530 540 550
GSLISDQWVL TAAHCFHDIQ MEDHHLWRVN VGDPTSQHGK EFLVEDVIIA
560 570 580 590 600
PGFNVHAKRK QGISEFYADD IALLKLSRKV KMSTHARPIC LPCTVGANMA
610 620 630 640 650
LRRSPGSTCK DHETELLSQQ KVPAHFVALN GNRLNINLRT GPEWTRCIQA
660 670 680 690 700
VSQNKNIFPS LTNVSEVVTD QFLCSGMEEE DDNPCKGESG GAVFLGRRYR
710 720 730 740 750
FFQVGLVSWG LFDPCHGSSN KNLRKKPPRG VLPRDFHISL FRLQPWLRQH
760
LDGVLDFLPL
Length:760
Mass (Da):84,741
Last modified:July 27, 2011 - v2
Checksum:i91C896A3EDC7D448
GO
Isoform Short (identifier: P21180-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     606-612: Missing.

Show »
Length:753
Mass (Da):84,013
Checksum:iC997F5CBA3A12278
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123 – 124EQ → DE in AAA37380 (PubMed:2229060).Curated2
Sequence conflicti123 – 124EQ → DE in AAA37381 (PubMed:2229060).Curated2
Sequence conflicti123 – 124EQ → DE in AAA63294 (PubMed:2229060).Curated2
Sequence conflicti299S → T in AAA37380 (PubMed:2229060).Curated1
Sequence conflicti299S → T in AAA37381 (PubMed:2229060).Curated1
Sequence conflicti299S → T in AAA63294 (PubMed:2229060).Curated1
Sequence conflicti344T → A in AAA37380 (PubMed:2229060).Curated1
Sequence conflicti344T → A in AAA37381 (PubMed:2229060).Curated1
Sequence conflicti344T → A in AAA63294 (PubMed:2229060).Curated1
Sequence conflicti359S → T in AAA37380 (PubMed:2229060).Curated1
Sequence conflicti359S → T in AAA37381 (PubMed:2229060).Curated1
Sequence conflicti359S → T in AAA63294 (PubMed:2229060).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005385606 – 612Missing in isoform Short. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57891 mRNA Translation: AAA63294.1
M60579
, M60563, M60564, M60565, M60566, M60567, M60568, M60569, M60570, M60571, M60572, M60573, M60574, M60575, M60605, M60576, M60577, M60578 Genomic DNA Translation: AAA37380.1
M60579
, M60563, M60564, M60565, M60566, M60567, M60568, M60569, M60570, M60571, M60572, M60573, M60574, M60575, M60605, M60576, M60577, M60578 Genomic DNA Translation: AAA37381.1
AK146812 mRNA Translation: BAE27452.1
AF109906 Genomic DNA Translation: AAC84162.1
CT025759 Genomic DNA Translation: CAX15332.1
CH466666 Genomic DNA Translation: EDL26734.1
BC011086 mRNA Translation: AAH11086.1
CCDSiCCDS28664.1 [P21180-1]
PIRiA38876 C2MS
RefSeqiNP_038512.2, NM_013484.2 [P21180-1]
UniGeneiMm.283217

Genome annotation databases

EnsembliENSMUST00000025230; ENSMUSP00000025230; ENSMUSG00000024371 [P21180-1]
GeneIDi12263
KEGGimmu:12263
UCSCiuc008cdz.2 mouse [P21180-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCO2_MOUSE
AccessioniPrimary (citable) accession number: P21180
Secondary accession number(s): O70350
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
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