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Reviewed, UniProtKB/Swiss-Prot P21170 (SPEA_ECOLI)

Last modified June 16, 2009. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Biosynthetic arginine decarboxylase
      Short name=ADC
    EC=4.1.1.19
Gene names
Name: speA
Ordered Locus Names: b2938, JW2905
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length658 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the biosynthesis of agmatine from arginine By similarity.

Catalytic activity

L-arginine = agmatine + CO2. HAMAP MF_01417

Cofactor

Pyridoxal phosphate. HAMAP MF_01417

Magnesium. HAMAP MF_01417

Pathway

Amino-acid degradation; L-arginine degradation via ADC pathway; agmatine from L-arginine: step 1/1. HAMAP MF_01417

Subunit structure

Homotetramer. HAMAP MF_01417

Subcellular location

Periplasm. Ref.1

Induction

By growth in an acidic enriched medium containing arginine (biodegradative form), by growth in minimal media at neutral pH (biosynthetic). Putrescine and spermidine repress the speA gene and feedback inhibit ADC. HAMAP MF_01417

Post-translational modification

Processed post-translationally to a 70 kDa mature form. HAMAP MF_01417

The N-terminus is blocked. HAMAP MF_01417

Miscellaneous

ADC can be found in two forms: biodegradative and biosynthetic. The biodegradative form may play a role in regulating pH by consuming proteins. HAMAP MF_01417

Sequence similarities

Belongs to the Orn/Lys/Arg decarboxylase class-II family. SpeA subfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 8.4. HAMAP MF_01417

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 658658Biosynthetic arginine decarboxylase HAMAP MF_01417
PRO_0000149960

Regions

Region307 – 31711Substrate-binding Potential

Amino acid modifications

Modified residue1271N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict2261A → R in AAA24646. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P21170-1 [UniParc].

Last modified August 29, 2003. Version 2.
Checksum: 22613CEA5F957CC3

FASTA65873,898
        10         20         30         40         50         60 
MSDDMSMGLP SSAGEHGVLR SMQEVAMSSQ EASKMLRTYN IAWWGNNYYD VNELGHISVC 

        70         80         90        100        110        120 
PDPDVPEARV DLAQLVKTRE AQGQRLPALF CFPQILQHRL RSINAAFKRA RESYGYNGDY 

       130        140        150        160        170        180 
FLVYPIKVNQ HRRVIESLIH SGEPLGLEAG SKAELMAVLA HAGMTRSVIV CNGYKDREYI 

       190        200        210        220        230        240 
RLALIGEKMG HKVYLVIEKM SEIAIVLDEA ERLNVVPRLG VRARLASQGS GKWQSSGGEK 

       250        260        270        280        290        300 
SKFGLAATQV LQLVETLREA GRLDSLQLLH FHLGSQMANI RDIATGVRES ARFYVELHKL 

       310        320        330        340        350        360 
GVNIQCFDVG GGLGVDYEGT RSQSDCSVNY GLNEYANNII WAIGDACEEN GLPHPTVITE 

       370        380        390        400        410        420 
SGRAVTAHHT VLVSNIIGVE RNEYTVPTAP AEDAPRALQS MWETWQEMHE PGTRRSLREW 

       430        440        450        460        470        480 
LHDSQMDLHD IHIGYSSGIF SLQERAWAEQ LYLSMCHEVQ KQLDPQNRAH RPIIDELQER 

       490        500        510        520        530        540 
MADKMYVNFS LFQSMPDAWG IDQLFPVLPL EGLDQVPERR AVLLDITCDS DGAIDHYIDG 

       550        560        570        580        590        600 
DGIATTMPMP EYDPENPPML GFFMVGAYQE ILGNMHNLFG DTEAVDVFVF PDGSVEVELS 

       610        620        630        640        650 
DEGDTVADML QYVQLDPKTL LTQFRDQVKK TDLDAELQQQ FLEEFEAGLY GYTYLEDE 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and analysis of the speA gene encoding biosynthetic arginine decarboxylase in Escherichia coli."
Moore R.C., Boyle S.M.
J. Bacteriol. 172:4631-4640(1990) [PubMed: 2198270] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, PROTEOLYTIC PROCESSING.
Strain: K12.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Influence of cyclic AMP, agmatine, and a novel protein encoded by a flanking gene on speB (agmatine ureohydrolase) in Escherichia coli."
Szumanski M.B.W., Boyle S.M.
J. Bacteriol. 174:758-764(1992) [PubMed: 1310091] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 635-658.
Strain: K12.
[5]"Biosynthetic arginine decarboxylase from Escherichia coli. Purification and properties."
Wu W.H., Morris D.R.
J. Biol. Chem. 248:1687-1695(1973) [PubMed: 4571773] [Abstract]
Cited for: CHARACTERIZATION.
Strain: UW 44 / ATCC 27549.

Cross-references

Sequence databases

M31770 Genomic DNA. Translation: AAA24646.1.
U28377 Genomic DNA. Translation: AAA69105.1.
U00096 Genomic DNA. Translation: AAC75975.1.
AP009048 Genomic DNA. Translation: BAE77001.1.
M32363 Genomic DNA. No translation available.
PIRA65079.
RefSeqAP_003495.1.
NP_417413.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:307N.

Genome annotation databases

GeneID947432.
GenomeReviewsGene locus JW2905 in contig AP009048_GR.
Gene locus b2938 in contig U00096_GR.
KEGGecj:JW2905.
eco:b2938.

Organism-specific databases

EchoBASEEB0952.
EcoGeneEG10959. speA.
CMRSearch...

Phylogenomic databases

HOGENOMP21170.
OMAP21170. LICNGYK.

Enzyme and pathway databases

BioCycEcoCyc:ARGDECARBOXBIO-MON.
MetaCyc:ARGDECARBOXBIO-MON.

Family and domain databases

HAMAPMF_01417.
[Tree]
InterProIPR002985. Arg_decrbxlase.
IPR000183. De-COase2.
[Graphical view]
PANTHERPTHR11482:SF3. Arg_decrbxlase. 1 hit.
PfamPF02784. Orn_Arg_deC_N. 1 hit.
PF00278. Orn_DAP_Arg_deC. 1 hit.
[Graphical view]
PIRSFPIRSF001336. Arg_decrbxlase. 1 hit.
PRINTSPR01180. ARGDCRBXLASE.
PR01179. ODADCRBXLASE.
TIGRFAMsTIGR01273. speA. 1 hit.
PROSITEPS00878. ODR_DC_2_1. 1 hit.
PS00879. ODR_DC_2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPEA_ECOLI
AccessionPrimary (citable) accession number: P21170
Secondary accession number(s): Q2M9Q5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 29, 2003
Last modified: June 16, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Recent format changes

Overview of recent format changes

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents