P21161 (DCMA_METED) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dichloromethane dehalogenase
Short name=DCM dehalogenase
EC=4.5.1.3

Gene names

Name:	dcmA
Ordered Locus Names:	METDI2656

Organism

Methylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium dichloromethanicum (strain DM4)) [Complete proteome] [HAMAP]

Taxonomic identifier

661410 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Dichloromethane + H2O = formaldehyde + 2 HCl.
Pathway	Xenobiotic degradation; dichloromethane degradation.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm Probable.
Induction	By dichloromethane.
Sequence similarities	Belongs to the GST superfamily. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence CAX24312.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dichloromethane dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Dichloromethane dehalogenase		PRO_0000185990
Regions
	Domain	12 – 94	83	GST N-terminal
	Domain	100 – 252	153	GST C-terminal
Natural variations
	Natural variant	1	1	Missing in 50 to 66% of the proteins.

Sequences

	Sequence		Length	Mass (Da)
	P21161 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 3B79D2C3424FDF11	FASTA	288	33,112
	10 20 30 40 50 60 MSPNPTNIHT GKTLRLLYHP ASQPCRSAHQ FMYEIDVPFE EEVVDISTDI TERQEFRDKY 70 80 90 100 110 120 NPTGQVPILV DGEFTVWESV AIARYVNEKF DGAGNWFGRG TQERAQINQF LQWYAYTLRL 130 140 150 160 170 180 GGGAFHWNIF GCLIYGEKPY SPKFTAEQNK GRTLLYEAMG TLENYWLRDR EYVCGDEVSY 190 200 210 220 230 240 ADLAAFHEFV SHEAGKIIPD RVWQGFPKIA AWFKKLSERP HAKTVSEWQY TNVGKIIRGE 250 260 270 280 LTASMFKRKT AVLKGTEVFS GHNHGIPYLN EKAEDYFKRV EKEGAAVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis and expression of the bacterial dichloromethane dehalogenase structural gene, a member of the glutathione S-transferase supergene family." la Roche S.D., Leisinger T. J. Bacteriol. 172:164-171(1990) [PubMed: 2104602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources." Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E. PLoS ONE 4:E5584-E5584(2009) [PubMed: 19440302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5838 / DM4.
[3]	"Evidence for identical dichloromethane dehalogenases in different methylotrophic bacteria." Kohler-Staub D., Hartmans S., Gaelli R., Suter F., Leisinger T. J. Gen. Microbiol. 132:2837-2843(1986) Cited for: PROTEIN SEQUENCE OF 1-15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32346 Genomic DNA. Translation: AAB68954.1. FP103042 Genomic DNA. Translation: CAX24312.1. Different initiation.
PIR	A47654.
RefSeq	YP_003068182.1. NC_012988.1.
3D structure databases
ProteinModelPortal	P21161.
ModBase	Search...
Protein-protein interaction databases
STRING	P21161.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8195277.
GenomeReviews	Gene locus METDI2656 in contig FP103042_GR.
KEGG	mdi:METDI2656.
Organism-specific databases
CMR	Search...
Phylogenomic databases
ProtClustDB	CLSK2815710.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCMA_METED

Accession

Primary (citable) accession number: P21161
Secondary accession number(s): C7CG48

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	July 27, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program