Reviewed, UniProtKB/Swiss-Prot P21156 (CYSD_ECOLI)

Names and origin

Protein names

Recommended name:
    Sulfate adenylyltransferase subunit 2
    EC=2.7.7.4
Alternative name(s):
    Sulfate adenylate transferase
      Short name=SAT
    ATP-sulfurylase small subunit

Gene names

Name:	cysD
Ordered Locus Names:	b2752, JW2722

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Enzyme regulation	Stimulated by an intrinsic GTPase (Probably cysN). HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of cysD, the smaller subunit, and cysN. HAMAP MF_00064
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000100664
Experimental info
	Sequence conflict	180	1	S → T in AAA23645. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21156-1 [UniParc]. Last modified June 20, 2003. Version 3. Checksum: A48881C0E5E086A1	FASTA	302	35,188
	10 20 30 40 50 60 MDQIRLTHLR QLEAESIHII REVAAEFSNP VMLYSIGKDS SVMLHLARKA FYPGTLPFPL 70 80 90 100 110 120 LHVDTGWKFR EMYEFRDRTA KAYGCELLVH KNPEGVAMGI NPFVHGSAKH TDIMKTEGLK 130 140 150 160 170 180 QALNKYGFDA AFGGARRDEE KSRAKERIYS FRDRFHRWDP KNQRPELWHN YNGQINKGES 190 200 210 220 230 240 IRVFPLSNWT EQDIWQYIWL ENIDIVPLYL AAERPVLERD GMLMMIDDNR IDLQPGEVIK 250 260 270 280 290 300 KRMVRFRTLG CWPLTGAVES NAQTLPEIIE EMLVSTTSER QGRVIDRDQA GSMELKKRQG YF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of the sulfate activation locus from Escherichia coli K-12." Leyh T.S., Vogt T.F., Suo Y. J. Biol. Chem. 267:10405-10410(1992) [PubMed: 1316900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Promoter elements and regulation of expression of the cysD gene of Escherichia coli K-12." Malo M.S., Loughlin R.E. Gene 87:127-131(1990) [PubMed: 2185135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27. Strain: K12.
[5]	"The sulfate activation locus of Escherichia coli K12: cloning, genetic, and enzymatic characterization." Leyh T.S., Taylor J.C., Markham G.D. J. Biol. Chem. 263:2409-2416(1988) [PubMed: 2828368] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M74586 Genomic DNA. Translation: AAA23645.1. U29579 Genomic DNA. Translation: AAA69262.1. U00096 Genomic DNA. Translation: AAC75794.1. AP009048 Genomic DNA. Translation: BAE76829.1. M35098 Genomic DNA. Translation: AAA74892.1.
PIR	D65056.
RefSeq	AP_003319.1. NP_417232.1.
3D structure databases
SMR	P21156. Positions 5-212.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:360N. DIP:9376N.
2-D gel databases
ECO2DBASE	H036.0. 6TH EDITION.
Genome annotation databases
GeneID	947217.
GenomeReviews	Gene locus JW2722 in contig AP009048_GR. Gene locus b2752 in contig U00096_GR.
KEGG	ecj:JW2722. eco:b2752.
Organism-specific databases
EchoBASE	EB0183.
EcoGene	EG10186. cysD.
CMR	Search...
Phylogenomic databases
HOGENOM	P21156.
OMA	P21156. KTSERQG.
Enzyme and pathway databases
BioCyc	EcoCyc:CYSD-MON. MetaCyc:CYSD-MON.
Family and domain databases
HAMAP	MF_00064. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_ECOLI

Accession

Primary (citable) accession number: P21156
Secondary accession number(s): Q2MA77

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	June 20, 2003
Last modified:	June 16, 2009
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)