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Protein

Sulfate adenylyltransferase subunit 2

Gene

cysD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.

Enzyme regulationi

Stimulated by an intrinsic GTPase (Probably CysN).

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • sulfate adenylyltransferase (ATP) activity Source: EcoCyc

GO - Biological processi

  • hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  • response to oxidative stress Source: EcoCyc
  • sulfate reduction Source: InterPro
  • sulfur compound metabolic process Source: EcoCyc

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:CYSD-MONOMER
MetaCyc:CYSD-MONOMER
SABIO-RKiP21156
UniPathwayiUPA00140; UER00204

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 2 (EC:2.7.7.4)
Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name:
SAT
Gene namesi
Name:cysD
Ordered Locus Names:b2752, JW2722
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10186 cysD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001006641 – 302Sulfate adenylyltransferase subunit 2Add BLAST302

Proteomic databases

PaxDbiP21156
PRIDEiP21156

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.

Protein-protein interaction databases

BioGridi4263040, 26 interactors
DIPiDIP-360N
IntActiP21156, 5 interactors
STRINGi316385.ECDH10B_2920

Structurei

3D structure databases

ProteinModelPortaliP21156
SMRiP21156
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysD subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C11 Bacteria
COG0175 LUCA
HOGENOMiHOG000263604
InParanoidiP21156
KOiK00957
OMAiFGQWQPK
PhylomeDBiP21156

Family and domain databases

CDDicd01713 PAPS_reductase, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00064 Sulf_adenylyltr_sub2, 1 hit
InterProiView protein in InterPro
IPR002500 PAPS_reduct
IPR014729 Rossmann-like_a/b/a_fold
IPR011784 SO4_adenylTrfase_ssu
PfamiView protein in Pfam
PF01507 PAPS_reduct, 1 hit
PIRSFiPIRSF002936 CysDAde_trans, 1 hit
TIGRFAMsiTIGR02039 CysD, 1 hit

Sequencei

Sequence statusi: Complete.

P21156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQIRLTHLR QLEAESIHII REVAAEFSNP VMLYSIGKDS SVMLHLARKA
60 70 80 90 100
FYPGTLPFPL LHVDTGWKFR EMYEFRDRTA KAYGCELLVH KNPEGVAMGI
110 120 130 140 150
NPFVHGSAKH TDIMKTEGLK QALNKYGFDA AFGGARRDEE KSRAKERIYS
160 170 180 190 200
FRDRFHRWDP KNQRPELWHN YNGQINKGES IRVFPLSNWT EQDIWQYIWL
210 220 230 240 250
ENIDIVPLYL AAERPVLERD GMLMMIDDNR IDLQPGEVIK KRMVRFRTLG
260 270 280 290 300
CWPLTGAVES NAQTLPEIIE EMLVSTTSER QGRVIDRDQA GSMELKKRQG

YF
Length:302
Mass (Da):35,188
Last modified:June 20, 2003 - v3
Checksum:iA48881C0E5E086A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180S → T in AAA23645 (PubMed:1316900).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74586 Genomic DNA Translation: AAA23645.1
U29579 Genomic DNA Translation: AAA69262.1
U00096 Genomic DNA Translation: AAC75794.1
AP009048 Genomic DNA Translation: BAE76829.1
M35098 Genomic DNA Translation: AAA74892.1
PIRiD65056
RefSeqiNP_417232.1, NC_000913.3
WP_000372108.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75794; AAC75794; b2752
BAE76829; BAE76829; BAE76829
GeneIDi947217
KEGGiecj:JW2722
eco:b2752
PATRICifig|1411691.4.peg.3988

Similar proteinsi

Entry informationi

Entry nameiCYSD_ECOLI
AccessioniPrimary (citable) accession number: P21156
Secondary accession number(s): Q2MA77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 20, 2003
Last modified: March 28, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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