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Protein

3-ketoacyl-CoA thiolase FadA

Gene

fadA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed. Involved in the aerobic and anaerobic degradation of long-chain fatty acids.2 Publications

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Kineticsi

  1. KM=96 µM for acetoacetyl-CoA1 Publication
  2. KM=102 µM for CoASH1 Publication

    Pathwayi: fatty acid beta-oxidation

    This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei91Acyl-thioester intermediateBy similarity1
    Active sitei343Proton acceptorBy similarity1
    Active sitei373Proton acceptorBy similarity1

    GO - Molecular functioni

    • acetyl-CoA C-acyltransferase activity Source: UniProtKB

    GO - Biological processi

    • fatty acid beta-oxidation Source: EcoCyc

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:FADA-MONOMER
    MetaCyc:FADA-MONOMER
    SABIO-RKiP21151
    UniPathwayiUPA00659

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-ketoacyl-CoA thiolase FadA (EC:2.3.1.16)
    Alternative name(s):
    Acetyl-CoA acyltransferase
    Beta-ketothiolase
    Fatty acid oxidation complex subunit beta
    Gene namesi
    Name:fadA
    Synonyms:oldA
    Ordered Locus Names:b3845, JW5578
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10278 fadA

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002063721 – 3873-ketoacyl-CoA thiolase FadAAdd BLAST387

    Proteomic databases

    EPDiP21151
    PaxDbiP21151
    PRIDEiP21151

    Expressioni

    Inductioni

    Repressed by FadR in the absence of LCFAs (fatty acids of, at least, 12 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs which bind to FadR resulting in its release from the DNA and thus derepression of the transcription.

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).1 Publication

    Protein-protein interaction databases

    BioGridi4263456, 162 interactors
    DIPiDIP-9559N
    STRINGi316385.ECDH10B_4034

    Structurei

    3D structure databases

    ProteinModelPortaliP21151
    SMRiP21151
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thiolase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CHU Bacteria
    COG0183 LUCA
    HOGENOMiHOG000012239
    InParanoidiP21151
    KOiK00632
    OMAiCGGTESM
    PhylomeDBiP21151

    Family and domain databases

    CDDicd00751 thiolase, 1 hit
    Gene3Di3.40.47.10, 4 hits
    HAMAPiMF_01620 FadA, 1 hit
    InterProiView protein in InterPro
    IPR012805 FadA
    IPR002155 Thiolase
    IPR016039 Thiolase-like
    IPR020615 Thiolase_acyl_enz_int_AS
    IPR020610 Thiolase_AS
    IPR020617 Thiolase_C
    IPR020613 Thiolase_CS
    IPR020616 Thiolase_N
    PfamiView protein in Pfam
    PF02803 Thiolase_C, 1 hit
    PF00108 Thiolase_N, 1 hit
    PIRSFiPIRSF000429 Ac-CoA_Ac_transf, 1 hit
    SUPFAMiSSF53901 SSF53901, 2 hits
    TIGRFAMsiTIGR01930 AcCoA-C-Actrans, 1 hit
    TIGR02445 fadA, 1 hit
    PROSITEiView protein in PROSITE
    PS00098 THIOLASE_1, 1 hit
    PS00737 THIOLASE_2, 1 hit
    PS00099 THIOLASE_3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P21151-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEQVVIVDAI RTPMGRSKGG AFRNVRAEDL SAHLMRSLLA RNPALEAAAL
    60 70 80 90 100
    DDIYWGCVQQ TLEQGFNIAR NAALLAEVPH SVPAVTVNRL CGSSMQALHD
    110 120 130 140 150
    AARMIMTGDA QACLVGGVEH MGHVPMSHGV DFHPGLSRNV AKAAGMMGLT
    160 170 180 190 200
    AEMLARMHGI SREMQDAFAA RSHARAWAAT QSAAFKNEII PTGGHDADGV
    210 220 230 240 250
    LKQFNYDEVI RPETTVEALA TLRPAFDPVN GMVTAGTSSA LSDGAAAMLV
    260 270 280 290 300
    MSESRAHELG LKPRARVRSM AVVGCDPSIM GYGPVPASKL ALKKAGLSAS
    310 320 330 340 350
    DIGVFEMNEA FAAQILPCIK DLGLIEQIDE KINLNGGAIA LGHPLGCSGA
    360 370 380
    RISTTLLNLM ERKDVQFGLA TMCIGLGQGI ATVFERV
    Length:387
    Mass (Da):40,876
    Last modified:September 27, 2004 - v3
    Checksum:i1A60EF1E6C351704
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti37S → T in CAB40810 (PubMed:2204034).Curated1
    Sequence conflicti37S → T in AAA67642 (PubMed:1379743).Curated1
    Sequence conflicti119E → G in CAB40810 (PubMed:2204034).Curated1
    Sequence conflicti371 – 374TMCI → DGCVS AA sequence (PubMed:2191949).Curated4

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M59368 Genomic DNA Translation: AAA23751.1
    M74164 Genomic DNA Translation: AAA62778.1
    X52837 Genomic DNA Translation: CAB40810.1
    M87049 Genomic DNA Translation: AAA67642.1
    U00096 Genomic DNA Translation: AAT48230.1
    AP009048 Genomic DNA Translation: BAE77458.1
    PIRiF65189 XUEC
    RefSeqiWP_000438725.1, NZ_LN832404.1
    YP_026272.1, NC_000913.3

    Genome annotation databases

    EnsemblBacteriaiAAT48230; AAT48230; b3845
    BAE77458; BAE77458; BAE77458
    GeneIDi948324
    KEGGiecj:JW5578
    eco:b3845
    PATRICifig|511145.12.peg.3959

    Similar proteinsi

    Entry informationi

    Entry nameiFADA_ECOLI
    AccessioniPrimary (citable) accession number: P21151
    Secondary accession number(s): P78130, Q2M8E8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: September 27, 2004
    Last modified: March 28, 2018
    This is version 154 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health