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P21147

- ACO1_YEAST

UniProt

P21147 - ACO1_YEAST

Protein

Acyl-CoA desaturase 1

Gene

OLE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Utilizes O2 and electrons from the reduced cytochrome b5 domain to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates.Curated

    Catalytic activityi

    Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.

    Cofactori

    Iron.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi444 – 4441Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi470 – 4701Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. electron carrier activity Source: SGD
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. stearoyl-CoA 9-desaturase activity Source: SGD

    GO - Biological processi

    1. unsaturated fatty acid biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17617.
    YEAST:YGL055W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-CoA desaturase 1 (EC:1.14.19.1)
    Alternative name(s):
    Fatty acid desaturase 1
    Stearoyl-CoA desaturase 1
    Gene namesi
    Name:OLE1
    Ordered Locus Names:YGL055W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL055w.
    SGDiS000003023. OLE1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 510510Acyl-CoA desaturase 1PRO_0000185406Add
    BLAST

    Proteomic databases

    MaxQBiP21147.
    PaxDbiP21147.
    PeptideAtlasiP21147.

    Expressioni

    Gene expression databases

    GenevestigatoriP21147.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-2098,EBI-2098
    AGP1P253761EBI-2098,EBI-2357
    CAB3P360761EBI-2098,EBI-26778
    CHO1P084561EBI-2098,EBI-14055
    EMC2P471331EBI-2098,EBI-25568
    ERG11P106141EBI-2098,EBI-5127
    ERG5P547811EBI-2098,EBI-6563
    ERV25P548371EBI-2098,EBI-6642
    GAP1P191451EBI-2098,EBI-7314
    GSF2Q046971EBI-2098,EBI-27807
    KRE27P405401EBI-2098,EBI-24977
    MID2P360271EBI-2098,EBI-10901
    OST1P415431EBI-2098,EBI-12651
    PGA3Q127461EBI-2098,EBI-27973
    PHO84P252971EBI-2098,EBI-13320
    PHO88P382641EBI-2098,EBI-13350
    PMP2P409751EBI-2098,EBI-2043041
    PPZ1P265701EBI-2098,EBI-13807
    RTN1Q049471EBI-2098,EBI-38020
    SAC1P323681EBI-2098,EBI-16210
    SEC63P149061EBI-2098,EBI-16636
    SPF1P399861EBI-2098,EBI-3128
    TPO4Q122561EBI-2098,EBI-37213
    UBP1P250371EBI-2098,EBI-19819
    VRG4P401071EBI-2098,EBI-7764
    YDR476CQ033621EBI-2098,EBI-2057915
    YET1P357231EBI-2098,EBI-26730

    Protein-protein interaction databases

    BioGridi33192. 103 interactions.
    DIPiDIP-5026N.
    IntActiP21147. 40 interactions.
    MINTiMINT-563591.
    STRINGi4932.YGL055W.

    Structurei

    3D structure databases

    ProteinModelPortaliP21147.
    SMRiP21147. Positions 407-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 112112CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini134 – 1385LumenalSequence Analysis
    Topological domaini160 – 25596CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini277 – 2804LumenalSequence Analysis
    Topological domaini302 – 510209CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei113 – 13321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei139 – 15921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei256 – 27621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei281 – 30121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini409 – 48779Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi161 – 1666Histidine box-1
    Motifi198 – 2025Histidine box-2
    Motifi335 – 3395Histidine box-3

    Domaini

    The histidine box domains may contain the active site and/or be involved in metal ion binding.

    Sequence similaritiesi

    Belongs to the fatty acid desaturase family.Curated
    Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1398.
    GeneTreeiENSGT00530000063158.
    HOGENOMiHOG000270353.
    KOiK00507.
    OMAiFNGGVYF.
    OrthoDBiEOG7S2277.

    Family and domain databases

    Gene3Di3.10.120.10. 1 hit.
    InterProiIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
    IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    IPR005804. Fatty_acid_desaturase-1.
    IPR001522. Fatty_acid_desaturase-1_C.
    IPR015876. Fatty_acid_desaturase-1_core.
    [Graphical view]
    PfamiPF00173. Cyt-b5. 1 hit.
    PF00487. FA_desaturase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000345. OLE1. 1 hit.
    PRINTSiPR00075. FACDDSATRASE.
    SUPFAMiSSF55856. SSF55856. 1 hit.
    PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    PS00476. FATTY_ACID_DESATUR_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21147-1 [UniParc]FASTAAdd to Basket

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    MPTSGTTIEL IDDQFPKDDS ASSGIVDEVD LTEANILATG LNKKAPRIVN    50
    GFGSLMGSKE MVSVEFDKKG NEKKSNLDRL LEKDNQEKEE AKTKIHISEQ 100
    PWTLNNWHQH LNWLNMVLVC GMPMIGWYFA LSGKVPLHLN VFLFSVFYYA 150
    VGGVSITAGY HRLWSHRSYS AHWPLRLFYA IFGCASVEGS AKWWGHSHRI 200
    HHRYTDTLRD PYDARRGLWY SHMGWMLLKP NPKYKARADI TDMTDDWTIR 250
    FQHRHYILLM LLTAFVIPTL ICGYFFNDYM GGLIYAGFIR VFVIQQATFC 300
    INSLAHYIGT QPFDDRRTPR DNWITAIVTF GEGYHNFHHE FPTDYRNAIK 350
    WYQYDPTKVI IYLTSLVGLA YDLKKFSQNA IEEALIQQEQ KKINKKKAKI 400
    NWGPVLTDLP MWDKQTFLAK SKENKGLVII SGIVHDVSGY ISEHPGGETL 450
    IKTALGKDAT KAFSGGVYRH SNAAQNVLAD MRVAVIKESK NSAIRMASKR 500
    GEIYETGKFF 510
    Length:510
    Mass (Da):58,403
    Last modified:October 1, 1996 - v2
    Checksum:iA6CC78DD4210ECCA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti67 – 671D → G in AAT93029. (PubMed:17322287)Curated
    Sequence conflicti304 – 3041L → M in AAA34826. (PubMed:1978720)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05676 Genomic DNA. Translation: AAA34826.1.
    Z72577 Genomic DNA. Translation: CAA96757.1.
    AY693010 Genomic DNA. Translation: AAT93029.1.
    BK006941 Genomic DNA. Translation: DAA08047.1.
    PIRiS64059.
    RefSeqiNP_011460.3. NM_001180920.3.

    Genome annotation databases

    EnsemblFungiiYGL055W; YGL055W; YGL055W.
    GeneIDi852825.
    KEGGisce:YGL055W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05676 Genomic DNA. Translation: AAA34826.1 .
    Z72577 Genomic DNA. Translation: CAA96757.1 .
    AY693010 Genomic DNA. Translation: AAT93029.1 .
    BK006941 Genomic DNA. Translation: DAA08047.1 .
    PIRi S64059.
    RefSeqi NP_011460.3. NM_001180920.3.

    3D structure databases

    ProteinModelPortali P21147.
    SMRi P21147. Positions 407-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33192. 103 interactions.
    DIPi DIP-5026N.
    IntActi P21147. 40 interactions.
    MINTi MINT-563591.
    STRINGi 4932.YGL055W.

    Proteomic databases

    MaxQBi P21147.
    PaxDbi P21147.
    PeptideAtlasi P21147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL055W ; YGL055W ; YGL055W .
    GeneIDi 852825.
    KEGGi sce:YGL055W.

    Organism-specific databases

    CYGDi YGL055w.
    SGDi S000003023. OLE1.

    Phylogenomic databases

    eggNOGi COG1398.
    GeneTreei ENSGT00530000063158.
    HOGENOMi HOG000270353.
    KOi K00507.
    OMAi FNGGVYF.
    OrthoDBi EOG7S2277.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-17617.
    YEAST:YGL055W-MONOMER.

    Miscellaneous databases

    NextBioi 972381.

    Gene expression databases

    Genevestigatori P21147.

    Family and domain databases

    Gene3Di 3.10.120.10. 1 hit.
    InterProi IPR009160. Acyl-CoA_deSatase_haem/ster-bd.
    IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    IPR005804. Fatty_acid_desaturase-1.
    IPR001522. Fatty_acid_desaturase-1_C.
    IPR015876. Fatty_acid_desaturase-1_core.
    [Graphical view ]
    Pfami PF00173. Cyt-b5. 1 hit.
    PF00487. FA_desaturase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000345. OLE1. 1 hit.
    PRINTSi PR00075. FACDDSATRASE.
    SUPFAMi SSF55856. SSF55856. 1 hit.
    PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    PS00476. FATTY_ACID_DESATUR_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The OLE1 gene of Saccharomyces cerevisiae encodes the delta 9 fatty acid desaturase and can be functionally replaced by the rat stearoyl-CoA desaturase gene."
      Stukey J.E., McDonough V.M., Martin C.E.
      J. Biol. Chem. 265:20144-20149(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
      Feuermann M., de Montigny J., Potier S., Souciet J.-L.
      Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiACO1_YEAST
    AccessioniPrimary (citable) accession number: P21147
    Secondary accession number(s): D6VU86, E9P911
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3