Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P21146

- ARBK1_BOVIN

UniProt

P21146 - ARBK1_BOVIN

Protein

Beta-adrenergic receptor kinase 1

Gene

ADRBK1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner By similarity.By similarity

    Catalytic activityi

    ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPPROSITE-ProRule annotation
    Active sitei317 – 3171Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. alpha-2A adrenergic receptor binding Source: BHF-UCL
    2. ATP binding Source: UniProtKB-KW
    3. beta-adrenergic receptor kinase activity Source: UniProtKB-EC
    4. Edg-2 lysophosphatidic acid receptor binding Source: UniProtKB
    5. G-protein coupled receptor kinase activity Source: BHF-UCL
    6. protein binding Source: IntAct
    7. protein kinase activity Source: BHF-UCL

    GO - Biological processi

    1. cardiac muscle contraction Source: BHF-UCL
    2. desensitization of G-protein coupled receptor protein signaling pathway Source: BHF-UCL
    3. G-protein coupled acetylcholine receptor signaling pathway Source: BHF-UCL
    4. G-protein coupled receptor internalization Source: UniProtKB
    5. G-protein coupled receptor signaling pathway Source: AgBase
    6. heart development Source: AgBase
    7. intracellular protein transport Source: BHF-UCL
    8. negative regulation of striated muscle contraction Source: BHF-UCL
    9. negative regulation of the force of heart contraction by chemical signal Source: BHF-UCL
    10. peptidyl-serine phosphorylation Source: BHF-UCL
    11. peptidyl-threonine phosphorylation Source: Ensembl
    12. protein phosphorylation Source: BHF-UCL
    13. regulation of signal transduction Source: BHF-UCL
    14. regulation of the force of heart contraction Source: AgBase
    15. tachykinin receptor signaling pathway Source: Ensembl
    16. termination of G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.15. 908.
    ReactomeiREACT_202422. Calmodulin induced events.
    REACT_209521. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-adrenergic receptor kinase 1 (EC:2.7.11.15)
    Short name:
    Beta-ARK-1
    Alternative name(s):
    G-protein-coupled receptor kinase 2
    Gene namesi
    Name:ADRBK1
    Synonyms:GRK2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: BHF-UCL
    2. membrane Source: BHF-UCL

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 689689Beta-adrenergic receptor kinase 1PRO_0000085626Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei670 – 6701PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP21146.

    Expressioni

    Tissue specificityi

    Ubiquitous; brain, spleen > heart, lung > kidney.

    Interactioni

    Subunit structurei

    Interacts with GIT1. Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1. Interacts with LPAR1 and LPAR2. Interacts with RALA in response to LPAR1 activation. ADRBK1 and RALA mutually inhibit each other's binding to LPAR1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HDAC6Q9UBN73EBI-1036401,EBI-301697From a different organism.
    Hdac6Q9Z2V52EBI-1036401,EBI-1009256From a different organism.

    Protein-protein interaction databases

    BioGridi159836. 3 interactions.
    DIPiDIP-37856N.
    IntActiP21146. 4 interactions.
    STRINGi9913.ENSBTAP00000050806.

    Structurei

    Secondary structure

    1
    689
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi39 – 4810
    Turni49 – 513
    Helixi55 – 595
    Helixi62 – 7514
    Turni77 – 793
    Helixi80 – 9314
    Helixi98 – 11215
    Helixi114 – 1185
    Helixi126 – 13712
    Turni143 – 1464
    Helixi147 – 15812
    Helixi160 – 1678
    Helixi169 – 18214
    Beta strandi191 – 1999
    Beta strandi201 – 21010
    Turni211 – 2133
    Beta strandi216 – 2238
    Helixi224 – 2307
    Helixi233 – 24412
    Beta strandi247 – 2493
    Beta strandi252 – 2543
    Beta strandi257 – 2626
    Beta strandi264 – 2729
    Helixi279 – 2868
    Helixi291 – 31020
    Helixi320 – 3223
    Beta strandi323 – 3253
    Beta strandi327 – 3293
    Beta strandi331 – 3333
    Helixi336 – 3383
    Beta strandi343 – 3453
    Helixi354 – 3563
    Helixi359 – 3624
    Helixi371 – 38616
    Beta strandi393 – 3953
    Helixi399 – 4068
    Beta strandi415 – 4173
    Helixi419 – 42810
    Turni433 – 4353
    Turni437 – 4393
    Beta strandi440 – 4423
    Helixi444 – 4485
    Helixi451 – 4533
    Helixi458 – 4625
    Helixi502 – 5043
    Turni505 – 5084
    Helixi514 – 5229
    Turni523 – 5253
    Helixi526 – 54621
    Turni553 – 5586
    Beta strandi561 – 5677
    Beta strandi577 – 5848
    Beta strandi587 – 5915
    Beta strandi593 – 5953
    Beta strandi599 – 6024
    Turni603 – 6053
    Beta strandi606 – 6149
    Beta strandi617 – 6248
    Turni625 – 6273
    Beta strandi629 – 6335
    Helixi637 – 66024
    Turni662 – 6643

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OMWX-ray2.50A1-689[»]
    1YM7X-ray4.50A/B/C/D1-689[»]
    2BCJX-ray3.06A1-689[»]
    3PSCX-ray2.67A1-689[»]
    3PVUX-ray2.48A1-689[»]
    3PVWX-ray2.49A1-689[»]
    3UZSX-ray4.52A1-681[»]
    3UZTX-ray3.51A1-689[»]
    ProteinModelPortaliP21146.
    SMRiP21146. Positions 28-667.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21146.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 175122RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini191 – 453263Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini454 – 52168AGC-kinase C-terminalAdd
    BLAST
    Domaini558 – 65295PHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 190190N-terminalAdd
    BLAST
    Regioni454 – 689236C-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000006742.
    HOVERGENiHBG050559.
    InParanoidiP21146.
    KOiK00910.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00233. PH. 1 hit.
    SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21146-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR    50
    GEVTFEKIFS QKLGYLLFRD FCLKHLEEAK PLVEFYEEIK KYEKLETEEE 100
    RLVCSREIFD TYIMKELLAC SHPFSKSAIE HVQGHLVKKQ VPPDLFQPYI 150
    EEICQNLRGD VFQKFIESDK FTRFCQWKNV ELNIHLTMND FSVHRIIGRG 200
    GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD 250
    CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE 300
    IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKKPHAS 350
    VGTHGYMAPE VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH 400
    EIDRMTLTMA VELPDSFSPE LRSLLEGLLQ RDVNRRLGCL GRGAQEVKES 450
    PFFRSLDWQM VFLQKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD 500
    SDQELYRNFP LTISERWQQE VAETVFDTIN AETDRLEARK KTKNKQLGHE 550
    EDYALGKDCI MHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RGEGEAPQSL 600
    LTMEEIQSVE ETQIKERKCL LLKIRGGKQF VLQCDSDPEL VQWKKELRDA 650
    YREAQQLVQR VPKMKNKPRS PVVELSKVPL IQRGSANGL 689
    Length:689
    Mass (Da):79,647
    Last modified:May 1, 1991 - v1
    Checksum:i4AB95DB5E630B9DA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34019 mRNA. Translation: AAA30384.1.
    PIRiA40088.
    RefSeqiNP_777135.1. NM_174710.2.
    UniGeneiBt.4693.

    Genome annotation databases

    GeneIDi282682.
    KEGGibta:282682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34019 mRNA. Translation: AAA30384.1 .
    PIRi A40088.
    RefSeqi NP_777135.1. NM_174710.2.
    UniGenei Bt.4693.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OMW X-ray 2.50 A 1-689 [» ]
    1YM7 X-ray 4.50 A/B/C/D 1-689 [» ]
    2BCJ X-ray 3.06 A 1-689 [» ]
    3PSC X-ray 2.67 A 1-689 [» ]
    3PVU X-ray 2.48 A 1-689 [» ]
    3PVW X-ray 2.49 A 1-689 [» ]
    3UZS X-ray 4.52 A 1-681 [» ]
    3UZT X-ray 3.51 A 1-689 [» ]
    ProteinModelPortali P21146.
    SMRi P21146. Positions 28-667.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 159836. 3 interactions.
    DIPi DIP-37856N.
    IntActi P21146. 4 interactions.
    STRINGi 9913.ENSBTAP00000050806.

    Proteomic databases

    PRIDEi P21146.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 282682.
    KEGGi bta:282682.

    Organism-specific databases

    CTDi 156.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000006742.
    HOVERGENi HBG050559.
    InParanoidi P21146.
    KOi K00910.

    Enzyme and pathway databases

    BRENDAi 2.7.11.15. 908.
    Reactomei REACT_202422. Calmodulin induced events.
    REACT_209521. G alpha (q) signalling events.

    Miscellaneous databases

    EvolutionaryTracei P21146.
    NextBioi 20806352.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00233. PH. 1 hit.
    SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Beta-adrenergic receptor kinase: primary structure delineates a multigene family."
      Benovic J.L., Deblasi A., Stone W.C., Caron M.G., Lefkowitz R.J.
      Science 246:235-240(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Beta2-adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein."
      Premont R.T., Claing A., Vitale N., Freeman J.L.R., Pitcher J.A., Patton W.A., Moss J., Vaughan M., Lefkowitz R.J.
      Proc. Natl. Acad. Sci. U.S.A. 95:14082-14087(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GIT1.

    Entry informationi

    Entry nameiARBK1_BOVIN
    AccessioniPrimary (citable) accession number: P21146
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3