Alpha-mannosidase 2C1 - Rattus norvegicus (Rat)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Alpha-mannosidase 2C1
EC=3.2.1.24

Alternative name(s):
Alpha-D-mannoside mannohydrolase
Mannosidase alpha class 2C member 1

Gene names

Name:

Man2c1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1040 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Endoplasmic reticulum.
Post-translational modification	A soluble isoform of the rat liver mannosidase is supposed to be derived from endoplasmic reticulum membrane alpha-mannosidase by proteolysis.
Sequence similarities	Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mannose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Non-traceable author statement Ref.1. Source: RGD
Molecular_function	alpha-mannosidase activity Inferred from direct assay Ref.1. Source: RGD carbohydrate binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1040

1040

Alpha-mannosidase 2C1

PRO_0000206909

Sites

Active site

371

1

Nucleophile By similarity

Metal binding

259

1

Zinc By similarity

Metal binding

261

1

Zinc By similarity

Metal binding

371

1

Zinc By similarity

Metal binding

576

1

Zinc By similarity

Amino acid modifications

Modified residue

481

1

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P21139 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: 144F6EB97C8F7EA7
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FASTA

1,040

115,971

        10         20         30         40         50         60 
MAAAPFLKHW RTTFERVEKF VSPIYFTDCN LRGRLFGDSC PVTLSSFLTP ERLPYEKAVQ 

        70         80         90        100        110        120 
QNFSPAQVGD SFGPTWWTCW FRVELVIPEV WVGKEVHLCW ESDGESLVWR DGEPVQGLTK 

       130        140        150        160        170        180 
EGEKTSYVLS ERLHAADPRS LTLYVEVACN GLLGAGKGSM IAAPDPEKMF QLSQAKLAVF 

       190        200        210        220        230        240 
HRDVHNLLVD LELLLGVAKG LGEDNQRSFQ ALYTANQMVN ICDPAQPETY PAAEALASKF 

       250        260        270        280        290        300 
FGQRGGESQH TIHATGHCHI DTAWLWPFKE TVRKCARSWS TAVKLMERNT EFTFACSQAQ 

       310        320        330        340        350        360 
QLEWVKNQYP GLYAQLQEFA CRGQFVPVGG TWVEMDGNLP SGEAMVRQFL QGQNFFLQEF 

       370        380        390        400        410        420 
GKMCSEFWLP DTFGYSAQLP QIMQGCGIKR FLTQKLSWNL VNSFPHHTFF WEGLDGSQVL 

       430        440        450        460        470        480 
VHFPPGDSYG MQGSVEEVLK TVTNNRDKGR TNHSGFLFGF GDGGGGPTQT MLDRLKRLGN 

       490        500        510        520        530        540 
TDGQPRVQLS SPGQLFTALE RDSGQLCTWV GELFLELHNG TYTTHAQLKK GNRECEQILH 

       550        560        570        580        590        600 
DVELLSSLAL ARSAQFLYPA VQLQRLWRLL LLNQFHDVVT GSCIQLVAED AMNYYEDIRS 

       610        620        630        640        650        660 
HGNTLLSAAA AALCAGEPGP KGLRHYQHTA LEAHRSVGTT QGLVGLTRLA LVTVPSIGYA 

       670        680        690        700        710        720 
PAPTPTSLQP LLPQQPVFVM QETDGSVTLD NGIIRVRLDP TGCLTSLVLV ASGREAIAEG 

       730        740        750        760        770        780 
ALGNQFVLFD DVPLYWDAWD VMDYHLETRK PVRGQAGTLA VGAEGGLRGS AWFLLQISPN 

       790        800        810        820        830        840 
SRLSQEVVLD VGCPYVRFHT EVHWHETHKF LKVEFPARVR SPQATYEIQF GHLQEADPQQ 

       850        860        870        880        890        900 
HSWDWARYEV WAHRWIDLSE CDFGLALLNN CKYGTSVRGN VLSLSLLRAP KAPDVTADMG 

       910        920        930        940        950        960 
RHEFTYALMP HKGSFQEAGV IQAAYNLNFP LLALPAPGPA PDTTWSAFSV SSPAVVLETI 

       970        980        990       1000       1010       1020 
KQAEKSHQHR TLVLRLYEAH GSHVDCWLHT SLPVQEATLC DLLEQRDPTG HLSLQDNRLK 

      1030       1040 
LTFSPFQVRS LLLVLQPPAN

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References

[1]	"Isolation, characterization, and expression of cDNA encoding a rat liver endoplasmic reticulum alpha-mannosidase." Bischoff J., Moremen K., Lodish H.F. J. Biol. Chem. 265:17110-17117(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57547 mRNA. Translation: AAA41565.1.
IPI	IPI00200121.
PIR	A38306.
RefSeq	NP_640349.1. NM_139256.1.
UniGene	Rn.11301.
3D structure databases
ProteinModelPortal	P21139.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3389982.
STRING	10116.ENSRNOP00000041867.
Protein family/group databases
CAZy	GH38. Glycoside Hydrolase Family 38.
Proteomic databases
PaxDb	P21139.
PRIDE	P21139.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	246136.
KEGG	rno:246136.
UCSC	RGD:628787. rat.
Organism-specific databases
CTD	4123.
RGD	628787. Man2c1.
Phylogenomic databases
eggNOG	COG0383.
HOGENOM	HOG000048425.
HOVERGEN	HBG055567.
InParanoid	P21139.
KO	K01191.
Enzyme and pathway databases
SABIO-RK	P21139.
Gene expression databases
ArrayExpress	P21139.
Genevestigator	P21139.
GermOnline	ENSRNOG00000030654. Rattus norvegicus.
Family and domain databases
Gene3D	3.20.110.10. 1 hit.
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR027291. Glyco_hydro_38/57_N. IPR011682. Glyco_hydro_38_C. IPR015341. Glyco_hydro_38_cen_dom. IPR000602. Glyco_hydro_38_N. [Graphical view]
Pfam	PF09261. Alpha-mann_mid. 1 hit. PF01074. Glyco_hydro_38. 1 hit. PF07748. Glyco_hydro_38C. 1 hit. [Graphical view]
SMART	SM00872. Alpha-mann_mid. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...
Other
BindingDB	P21139.
ChEMBL	CHEMBL2852.
NextBio	623390.

Entry information

Entry name

MA2C1_RAT

Accession

Primary (citable) accession number: P21139

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents