P21130 (SACB_BACAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Levansucrase
EC=2.4.1.10

Alternative name(s):
Beta-D-fructofuranosyl transferase
Sucrose 6-fructosyl transferase

Gene names

Name:

sacB

Organism

Bacillus amyloliquefaciens (Bacillus velezensis)

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside.
Subcellular location	Secreted.
Induction	By sucrose.
Sequence similarities	Belongs to the glycosyl hydrolase 68 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	carbohydrate utilization Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	levansucrase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29
	Chain	30 – 472	443	Levansucrase		PRO_0000012247

Sequences

	Sequence		Length	Mass (Da)
	P21130 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: F38592D272677E7D	FASTA	472	52,859
	10 20 30 40 50 60 MNIKKIVKQA TVLTFTTALL AGGATQAFAK ENNQKAYKET YGVSHITRHD MLQIPKQQQN 70 80 90 100 110 120 EKYQVPQFDQ STIKNIESAK GLDVWDSWPL QNADGTVAEY NGYHVVFALA GSPKDADDTS 130 140 150 160 170 180 IYMFYQKVGD NSIDSWKNAG RVFKDSDKFD ANDPILKDQT QEWSGSATFT SDGKIRLFYT 190 200 210 220 230 240 DYSGKHYGKQ SLTTAQVNVS KSDDTLKING VEDHKTIFDG DGKTYQNVQQ FIDEGNYTSG 250 260 270 280 290 300 DNHTLRDPHY VEDKGHKYLV FEANTGTENG YQGEESLFNK AYYGGGTNFF RKESQKLQQS 310 320 330 340 350 360 AKKRDAELAN GALGIIELNN DYTLKKVMKP LITSNTVTDE IERANVFKMN GKWYLFTDSR 370 380 390 400 410 420 GSKMTIDGIN SNDIYMLGYV SNSLTGPYKP LNKTGLVLQM GLDPNDVTFT YSHFAVPQAK 430 440 450 460 470 GNNVVITSYM TNRGFFEDKK ATFGPSFLMN IKGNKTSVVK NSILEQGQLT VN

References

[1]	"Isolation and characterization of levansucrase-encoding gene from Bacillus amyloliquefaciens." Tang L.B., Lenstra R., Borchert T.V., Vasantha N. Gene 96:89-93(1990) [PubMed: 2265762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23844 / P.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52988 Genomic DNA. Translation: CAA37179.1.
PIR	JQ0802.
3D structure databases
ProteinModelPortal	P21130.
SMR	P21130. Positions 34-472.
ModBase	Search...
Protein family/group databases
CAZy	GH68. Glycoside Hydrolase Family 68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR023296. Glyco_hydro_43_beta-prop. IPR003469. Glyco_hydro_68. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 2 hits.
Pfam	PF02435. Glyco_hydro_68. 1 hit. [Graphical view]
SUPFAM	SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SACB_BACAM

Accession

Primary (citable) accession number: P21130

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	December 14, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program