Reviewed, UniProtKB/Swiss-Prot P21127 (CD2L1_HUMAN)

Names and origin

Protein names

Recommended name:
    PITSLRE serine/threonine-protein kinase CDC2L1
    EC=2.7.11.22
Alternative name(s):
    Cell division cycle 2-like protein kinase 1
      Short name=CLK-1
    p58 CLK-1
    Galactosyltransferase-associated protein kinase p58/GTA
    CDK11

Gene names

Name:	CDC2L1
Synonyms:	PITSLREA, PK58

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Appears to play multiple roles in cell cycle progression, cytokinesis and apoptosis. The p110 isoforms have been suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. The p58 isoform may act as a negative regulator of normal cell cycle progression. Ref.1 Ref.11 Ref.12
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Enzyme regulation	Phosphorylation at Thr-448 or Tyr-449 inactivates the enzyme, while phosphorylation at Thr-595 activates it By similarity.
Subunit structure	The cleaved p110 isoform, p110C, binds to the serine/threonine kinase PAK1 and RANBP9. The cleaved p110 isoform interacts with RNPS1. The p58 isoform but not the p110 isoforms or p110C interacts with CCND3. The p110 isoforms are found in large molecular weight complexes containing CCNL1 and SFRS7. Ref.11 Ref.12 Ref.8 Ref.10
Subcellular location	Cytoplasm. Nucleus. Ref.11 Ref.10 Ref.4
Tissue specificity	Expressed ubiquitously. Some evidence of isoform-specific tissue distribution. Ref.4 Ref.5
Induction	The p58 isoform is specifically induced in G2/M phase of the cell cycle. Ref.9
Post-translational modification	During apoptosis, induced by Fas or tumor necrosis factor, specific PITSLRE p110 isoforms are cleaved by caspases to produce a protein (p110C) that contains the C-terminal kinase domain of the PITSLRE proteins. P110C can be autophosphorylated. Ref.10 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17
Miscellaneous	Duplicated gene. CDC2L1 and CDC2L2 encode almost identical protein kinases of 110 kDa that contain at their C termini the open reading frame of a smaller 58 kDa isoform which is expressed following IRES-mediated alternative initiation of translation.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Caution	Many references talk about 'p110 isoforms' but it is not yet known if this refers to CDC2L1 and/or CDC2L2 or one/some of the isoforms of each.
Sequence caution	The sequence AAC83664.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Apoptosis Cell cycle
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative initiation Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	apoptosis Ref.5 Non-traceable author statement. Source: UniProtKB cell proliferation Ref.4 Traceable author statement. Source: ProtInc mitosis Ref.3 Non-traceable author statement. Source: UniProtKB protein amino acid phosphorylation Ref.3 Ref.11 Inferred from direct assay. Source: UniProtKB regulation of cell growth Ref.9 Inferred from expression pattern. Source: UniProtKB regulation of mRNA processing Ref.11 Inferred from direct assay. Source: UniProtKB regulation of transcription, DNA-dependent Ref.5 Non-traceable author statement. Source: UniProtKB
Cellular component	cytoplasm Ref.4 Traceable author statement. Source: ProtInc nucleus Ref.4 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Ref.11 Inferred from direct assay. Source: UniProtKB cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC protein binding Ref.12 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAK1	Q13153	2	EBI-1298,EBI-1307
RNPS1	Q15287	1	EBI-1298,EBI-395959

Alternative products

This entry describes 10 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform SV9 (identifier: P21127-1)

Also known as: p110;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform SV1 (identifier: P21127-2)

Also known as: Alpha 2-1;

The sequence of this isoform differs from the canonical sequence as follows:
     252-265: GEARPARAQKPAQL → V

Isoform 2 (identifier: P21127-3)

Also known as: Alpha 2-2;

The sequence of this isoform differs from the canonical sequence as follows:
     110-119: Missing.
     165-165: R → RGNDGVCLFR
     252-265: GEARPARAQKPAQL → V

Isoform 3 (identifier: P21127-4)

Also known as: Alpha 1;

The sequence of this isoform differs from the canonical sequence as follows:
     2-335: Missing.

Isoform SV4 (identifier: P21127-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-269: Missing.

Isoform SV5 (identifier: P21127-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.
     35-37: LKN → MSQ
     110-119: Missing.
     252-265: GEARPARAQKPAQL → V

Isoform 8 (identifier: P21127-8)

Also known as: Alpha 2-3;

The sequence of this isoform differs from the canonical sequence as follows:
     110-119: Missing.
     252-265: GEARPARAQKPAQL → V

Isoform SV10 (identifier: P21127-9)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.
     35-37: LKN → MSQ
     252-265: GEARPARAQKPAQL → V

Isoform SV11 (identifier: P21127-10)

Also known as: Alpha 2-4;

The sequence of this isoform differs from the canonical sequence as follows:
     1-217: Missing.
     252-265: GEARPARAQKPAQL → V

Isoform 7 (identifier: P21127-12)

Also known as: p58;

The sequence of this isoform differs from the canonical sequence as follows:
     1-356: Missing.

Note: Produced by alternative initiation at Met-357 of isoform SV9.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 795	795	PITSLRE serine/threonine-protein kinase CDC2L1		PRO_0000024311
Regions
	Domain	438 – 723	286	Protein kinase
	Nucleotide binding	444 – 452	9	ATP By similarity
	Compositional bias	126 – 393	268	Glu-rich
Sites
	Active site	562	1	Proton acceptor By similarity
	Binding site	467	1	ATP By similarity
Amino acid modifications
	Modified residue	65	1	Phosphoserine Ref.17
	Modified residue	283	1	Phosphoserine Ref.13 Ref.15
	Modified residue	381	1	Phosphoserine Ref.17
	Modified residue	384	1	Phosphoserine Ref.17
	Modified residue	395	1	Phosphothreonine Ref.17
	Modified residue	410	1	Phosphoserine Ref.17
	Modified residue	414	1	Phosphoserine Ref.17
	Modified residue	448	1	Phosphothreonine By similarity
	Modified residue	449	1	Phosphotyrosine By similarity
	Modified residue	589	1	Phosphoserine Ref.13
	Modified residue	594	1	Phosphotyrosine Ref.17
	Modified residue	595	1	Phosphothreonine Ref.13 Ref.14 Ref.15 Ref.16 Ref.17
	Modified residue	751	1	Phosphothreonine By similarity
	Modified residue	752	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 356	356	Missing in isoform 7.		VSP_018834
	Alternative sequence	1 – 269	269	Missing in isoform SV4.		VSP_008275
	Alternative sequence	1 – 217	217	Missing in isoform SV11.		VSP_008274
	Alternative sequence	1 – 34	34	Missing in isoform SV5 and isoform SV10.		VSP_008273
	Alternative sequence	2 – 335	334	Missing in isoform 3.		VSP_008276
	Alternative sequence	35 – 37	3	LKN → MSQ in isoform SV5 and isoform SV10.		VSP_008277
	Alternative sequence	110 – 119	10	Missing in isoform 2, isoform SV5 and isoform 8.		VSP_008278
	Alternative sequence	165	1	R → RGNDGVCLFR in isoform 2.		VSP_008279
	Alternative sequence	252 – 265	14	GEARP…KPAQL → V in isoform SV1, isoform 2, isoform SV5, isoform 8, isoform SV10 and isoform SV11.		VSP_008280
	Natural variant	57	1	R → C Ref.4 Ref.18		VAR_041958
	Natural variant	93	1	R → W: dbSNP rs1059831.		VAR_057775
	Natural variant	201	1	R → W Ref.18		VAR_041959
	Natural variant	414	1	S → L Ref.18		VAR_041960
	Natural variant	452	1	V → A Ref.18		VAR_045577
	Natural variant	463	1	I → V Ref.18		VAR_041961
	Natural variant	506	1	G → S Ref.18		VAR_045578
	Natural variant	601	1	L → Q Ref.18 Ref.3		VAR_041962
	Natural variant	641	1	K → N Ref.18		VAR_041963
	Natural variant	670	1	A → V Ref.18		VAR_041964
Experimental info
	Sequence conflict	109	1	Missing in AAA19582. Ref.4
	Sequence conflict	109	1	Missing in AAA19583. Ref.4
	Sequence conflict	109	1	Missing in AAC72079. Ref.5
	Sequence conflict	126	1	E → K in AAA19582. Ref.4
	Sequence conflict	126	1	E → K in AAA19583. Ref.4
	Sequence conflict	126	1	E → K in AAA19586. Ref.4
	Sequence conflict	320	1	S → T Ref.6
	Sequence conflict	324 – 326	3	Missing in AAA19582. Ref.4
	Sequence conflict	324 – 326	3	Missing in AAA19583. Ref.4
	Sequence conflict	324 – 326	3	Missing in AAA19584. Ref.4
	Sequence conflict	324 – 326	3	Missing in AAA19586. Ref.4
	Sequence conflict	436	1	E → D Ref.3
	Sequence conflict	560	1	H → Q Ref.6
	Sequence conflict	567	1	N → T Ref.6
	Sequence conflict	678	1	E → R Ref.4
	Sequence conflict	678	1	E → R Ref.6
	Sequence conflict	694	1	D → E Ref.3
	Sequence conflict	697	1	F → C Ref.6
	Sequence conflict	712	1	I → L Ref.3
	Sequence conflict	715	1	E → Q Ref.3
	Sequence conflict	792	1	S → R Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform SV9 (p110) [UniParc]. Last modified September 19, 2003. Version 3. Checksum: C07CEE9A4F7A6804	FASTA	795	92,707
	10 20 30 40 50 60 MGDEKDSWKV KTLDEILQEK KRRKEQEEKA EIKRLKNSDD RDSKRDSLEE GELRDHRMEI 70 80 90 100 110 120 TIRNSPYRRE DSMEDRGEED DSLAIKPPQQ MSRKEKVHHR KDEKRKEKRR HRSHSAEGGK 130 140 150 160 170 180 HARVKEKERE HERRKRHREE QDKARREWER QKRREMAREH SRRERDRLEQ LERKRERERK 190 200 210 220 230 240 MREQQKEQRE QKERERRAEE RRKEREARRE VSAHHRTMRE DYSDKVKASH WSRSPPRPPR 250 260 270 280 290 300 ERFELGDGRK PGEARPARAQ KPAQLKEEKM EERDLLSDLQ DISDSERKTS SAESSSAESG 310 320 330 340 350 360 SGSEEEEEEE EEEEEEGSTS EESEEEEEEE EEEEEETGSN SEEASEQSAE EVSEEEMSED 370 380 390 400 410 420 EERENENHLL VVPESRFDRD SGESEEAEEE VGEGTPQSSA LTEGDYVPDS PALSPIELKQ 430 440 450 460 470 480 ELPKYLPALQ GCRSVEEFQC LNRIEEGTYG VVYRAKDKKT DEIVALKRLK MEKEKEGFPI 490 500 510 520 530 540 TSLREINTIL KAQHPNIVTV REIVVGSNMD KIYIVMNYVE HDLKSLMETM KQPFLPGEVK 550 560 570 580 590 600 TLMIQLLRGV KHLHDNWILH RDLKTSNLLL SHAGILKVGD FGLAREYGSP LKAYTPVVVT 610 620 630 640 650 660 LWYRAPELLL GAKEYSTAVD MWSVGCIFGE LLTQKPLFPG KSEIDQINKV FKDLGTPSEK 670 680 690 700 710 720 IWPGYSELPA VKKMTFSEHP YNNLRKRFGA LLSDQGFDLM NKFLTYFPGR RISAEDGLKH 730 740 750 760 770 780 EYFRETPLPI DPSMFPTWPA KSEQQRVKRG TSPRPPEGGL GYSQLGDDDL KETGFHLTTT 790 NQGASAAGPG FSLKF
	Isoform SV1 (Alpha 2-1). Checksum: 63A4E5BDBE1411FE	FASTA	782	91,331
	10 20 30 40 50 60 MGDEKDSWKV KTLDEILQEK KRRKEQEEKA EIKRLKNSDD RDSKRDSLEE GELRDHRMEI 70 80 90 100 110 120 TIRNSPYRRE DSMEDRGEED DSLAIKPPQQ MSRKEKVHHR KDEKRKEKRR HRSHSAEGGK 130 140 150 160 170 180 HARVKEKERE HERRKRHREE QDKARREWER QKRREMAREH SRRERDRLEQ LERKRERERK 190 200 210 220 230 240 MREQQKEQRE QKERERRAEE RRKEREARRE VSAHHRTMRE DYSDKVKASH WSRSPPRPPR 250 260 270 280 290 300 ERFELGDGRK PVKEEKMEER DLLSDLQDIS DSERKTSSAE SSSAESGSGS EEEEEEEEEE 310 320 330 340 350 360 EEEGSTSEES EEEEEEEEEE EEETGSNSEE ASEQSAEEVS EEEMSEDEER ENENHLLVVP 370 380 390 400 410 420 ESRFDRDSGE SEEAEEEVGE GTPQSSALTE GDYVPDSPAL SPIELKQELP KYLPALQGCR 430 440 450 460 470 480 SVEEFQCLNR IEEGTYGVVY RAKDKKTDEI VALKRLKMEK EKEGFPITSL REINTILKAQ 490 500 510 520 530 540 HPNIVTVREI VVGSNMDKIY IVMNYVEHDL KSLMETMKQP FLPGEVKTLM IQLLRGVKHL 550 560 570 580 590 600 HDNWILHRDL KTSNLLLSHA GILKVGDFGL AREYGSPLKA YTPVVVTLWY RAPELLLGAK 610 620 630 640 650 660 EYSTAVDMWS VGCIFGELLT QKPLFPGKSE IDQINKVFKD LGTPSEKIWP GYSELPAVKK 670 680 690 700 710 720 MTFSEHPYNN LRKRFGALLS DQGFDLMNKF LTYFPGRRIS AEDGLKHEYF RETPLPIDPS 730 740 750 760 770 780 MFPTWPAKSE QQRVKRGTSP RPPEGGLGYS QLGDDDLKET GFHLTTTNQG ASAAGPGFSL KF
	Isoform 2 (Alpha 2-2). Checksum: EDEE50FC3D19E26D	FASTA	781	91,218
	10 20 30 40 50 60 MGDEKDSWKV KTLDEILQEK KRRKEQEEKA EIKRLKNSDD RDSKRDSLEE GELRDHRMEI 70 80 90 100 110 120 TIRNSPYRRE DSMEDRGEED DSLAIKPPQQ MSRKEKVHHR KDEKRKEKRK HARVKEKERE 130 140 150 160 170 180 HERRKRHREE QDKARREWER QKRREMAREH SRRERGNDGV CLFRDRLEQL ERKRERERKM 190 200 210 220 230 240 REQQKEQREQ KERERRAEER RKEREARREV SAHHRTMRED YSDKVKASHW SRSPPRPPRE 250 260 270 280 290 300 RFELGDGRKP VKEEKMEERD LLSDLQDISD SERKTSSAES SSAESGSGSE EEEEEEEEEE 310 320 330 340 350 360 EEGSTSEESE EEEEEEEEEE EETGSNSEEA SEQSAEEVSE EEMSEDEERE NENHLLVVPE 370 380 390 400 410 420 SRFDRDSGES EEAEEEVGEG TPQSSALTEG DYVPDSPALS PIELKQELPK YLPALQGCRS 430 440 450 460 470 480 VEEFQCLNRI EEGTYGVVYR AKDKKTDEIV ALKRLKMEKE KEGFPITSLR EINTILKAQH 490 500 510 520 530 540 PNIVTVREIV VGSNMDKIYI VMNYVEHDLK SLMETMKQPF LPGEVKTLMI QLLRGVKHLH 550 560 570 580 590 600 DNWILHRDLK TSNLLLSHAG ILKVGDFGLA REYGSPLKAY TPVVVTLWYR APELLLGAKE 610 620 630 640 650 660 YSTAVDMWSV GCIFGELLTQ KPLFPGKSEI DQINKVFKDL GTPSEKIWPG YSELPAVKKM 670 680 690 700 710 720 TFSEHPYNNL RKRFGALLSD QGFDLMNKFL TYFPGRRISA EDGLKHEYFR ETPLPIDPSM 730 740 750 760 770 780 FPTWPAKSEQ QRVKRGTSPR PPEGGLGYSQ LGDDDLKETG FHLTTTNQGA SAAGPGFSLK F
	Isoform 3 (Alpha 1). Checksum: 6D054208C50D621C	FASTA	461	51,925
	10 20 30 40 50 60 METGSNSEEA SEQSAEEVSE EEMSEDEERE NENHLLVVPE SRFDRDSGES EEAEEEVGEG 70 80 90 100 110 120 TPQSSALTEG DYVPDSPALS PIELKQELPK YLPALQGCRS VEEFQCLNRI EEGTYGVVYR 130 140 150 160 170 180 AKDKKTDEIV ALKRLKMEKE KEGFPITSLR EINTILKAQH PNIVTVREIV VGSNMDKIYI 190 200 210 220 230 240 VMNYVEHDLK SLMETMKQPF LPGEVKTLMI QLLRGVKHLH DNWILHRDLK TSNLLLSHAG 250 260 270 280 290 300 ILKVGDFGLA REYGSPLKAY TPVVVTLWYR APELLLGAKE YSTAVDMWSV GCIFGELLTQ 310 320 330 340 350 360 KPLFPGKSEI DQINKVFKDL GTPSEKIWPG YSELPAVKKM TFSEHPYNNL RKRFGALLSD 370 380 390 400 410 420 QGFDLMNKFL TYFPGRRISA EDGLKHEYFR ETPLPIDPSM FPTWPAKSEQ QRVKRGTSPR 430 440 450 460 PPEGGLGYSQ LGDDDLKETG FHLTTTNQGA SAAGPGFSLK F
	Isoform SV4. Checksum: 3DFF4854ED82B81B	FASTA	526	59,273
	10 20 30 40 50 60 MEERDLLSDL QDISDSERKT SSAESSSAES GSGSEEEEEE EEEEEEEGST SEESEEEEEE 70 80 90 100 110 120 EEEEEEETGS NSEEASEQSA EEVSEEEMSE DEERENENHL LVVPESRFDR DSGESEEAEE 130 140 150 160 170 180 EVGEGTPQSS ALTEGDYVPD SPALSPIELK QELPKYLPAL QGCRSVEEFQ CLNRIEEGTY 190 200 210 220 230 240 GVVYRAKDKK TDEIVALKRL KMEKEKEGFP ITSLREINTI LKAQHPNIVT VREIVVGSNM 250 260 270 280 290 300 DKIYIVMNYV EHDLKSLMET MKQPFLPGEV KTLMIQLLRG VKHLHDNWIL HRDLKTSNLL 310 320 330 340 350 360 LSHAGILKVG DFGLAREYGS PLKAYTPVVV TLWYRAPELL LGAKEYSTAV DMWSVGCIFG 370 380 390 400 410 420 ELLTQKPLFP GKSEIDQINK VFKDLGTPSE KIWPGYSELP AVKKMTFSEH PYNNLRKRFG 430 440 450 460 470 480 ALLSDQGFDL MNKFLTYFPG RRISAEDGLK HEYFRETPLP IDPSMFPTWP AKSEQQRVKR 490 500 510 520 GTSPRPPEGG LGYSQLGDDD LKETGFHLTT TNQGASAAGP GFSLKF
	Isoform SV5. Checksum: 0885FB5ECECB398A	FASTA	738	86,063
	10 20 30 40 50 60 MSQSDDRDSK RDSLEEGELR DHRMEITIRN SPYRREDSME DRGEEDDSLA IKPPQQMSRK 70 80 90 100 110 120 EKVHHRKDEK RKEKRKHARV KEKEREHERR KRHREEQDKA RREWERQKRR EMAREHSRRE 130 140 150 160 170 180 RDRLEQLERK RERERKMREQ QKEQREQKER ERRAEERRKE REARREVSAH HRTMREDYSD 190 200 210 220 230 240 KVKASHWSRS PPRPPRERFE LGDGRKPVKE EKMEERDLLS DLQDISDSER KTSSAESSSA 250 260 270 280 290 300 ESGSGSEEEE EEEEEEEEEG STSEESEEEE EEEEEEEEET GSNSEEASEQ SAEEVSEEEM 310 320 330 340 350 360 SEDEERENEN HLLVVPESRF DRDSGESEEA EEEVGEGTPQ SSALTEGDYV PDSPALSPIE 370 380 390 400 410 420 LKQELPKYLP ALQGCRSVEE FQCLNRIEEG TYGVVYRAKD KKTDEIVALK RLKMEKEKEG 430 440 450 460 470 480 FPITSLREIN TILKAQHPNI VTVREIVVGS NMDKIYIVMN YVEHDLKSLM ETMKQPFLPG 490 500 510 520 530 540 EVKTLMIQLL RGVKHLHDNW ILHRDLKTSN LLLSHAGILK VGDFGLAREY GSPLKAYTPV 550 560 570 580 590 600 VVTLWYRAPE LLLGAKEYST AVDMWSVGCI FGELLTQKPL FPGKSEIDQI NKVFKDLGTP 610 620 630 640 650 660 SEKIWPGYSE LPAVKKMTFS EHPYNNLRKR FGALLSDQGF DLMNKFLTYF PGRRISAEDG 670 680 690 700 710 720 LKHEYFRETP LPIDPSMFPT WPAKSEQQRV KRGTSPRPPE GGLGYSQLGD DDLKETGFHL 730 TTTNQGASAA GPGFSLKF
	Isoform 8 (Alpha 2-3). Checksum: 03929873CFBD1CF6	FASTA	772	90,256
	10 20 30 40 50 60 MGDEKDSWKV KTLDEILQEK KRRKEQEEKA EIKRLKNSDD RDSKRDSLEE GELRDHRMEI 70 80 90 100 110 120 TIRNSPYRRE DSMEDRGEED DSLAIKPPQQ MSRKEKVHHR KDEKRKEKRK HARVKEKERE 130 140 150 160 170 180 HERRKRHREE QDKARREWER QKRREMAREH SRRERDRLEQ LERKRERERK MREQQKEQRE 190 200 210 220 230 240 QKERERRAEE RRKEREARRE VSAHHRTMRE DYSDKVKASH WSRSPPRPPR ERFELGDGRK 250 260 270 280 290 300 PVKEEKMEER DLLSDLQDIS DSERKTSSAE SSSAESGSGS EEEEEEEEEE EEEGSTSEES 310 320 330 340 350 360 EEEEEEEEEE EEETGSNSEE ASEQSAEEVS EEEMSEDEER ENENHLLVVP ESRFDRDSGE 370 380 390 400 410 420 SEEAEEEVGE GTPQSSALTE GDYVPDSPAL SPIELKQELP KYLPALQGCR SVEEFQCLNR 430 440 450 460 470 480 IEEGTYGVVY RAKDKKTDEI VALKRLKMEK EKEGFPITSL REINTILKAQ HPNIVTVREI 490 500 510 520 530 540 VVGSNMDKIY IVMNYVEHDL KSLMETMKQP FLPGEVKTLM IQLLRGVKHL HDNWILHRDL 550 560 570 580 590 600 KTSNLLLSHA GILKVGDFGL AREYGSPLKA YTPVVVTLWY RAPELLLGAK EYSTAVDMWS 610 620 630 640 650 660 VGCIFGELLT QKPLFPGKSE IDQINKVFKD LGTPSEKIWP GYSELPAVKK MTFSEHPYNN 670 680 690 700 710 720 LRKRFGALLS DQGFDLMNKF LTYFPGRRIS AEDGLKHEYF RETPLPIDPS MFPTWPAKSE 730 740 750 760 770 QQRVKRGTSP RPPEGGLGYS QLGDDDLKET GFHLTTTNQG ASAAGPGFSL KF
	Isoform SV10. Checksum: A13766F2F6F8E00A	FASTA	748	87,138
	10 20 30 40 50 60 MSQSDDRDSK RDSLEEGELR DHRMEITIRN SPYRREDSME DRGEEDDSLA IKPPQQMSRK 70 80 90 100 110 120 EKVHHRKDEK RKEKRRHRSH SAEGGKHARV KEKEREHERR KRHREEQDKA RREWERQKRR 130 140 150 160 170 180 EMAREHSRRE RDRLEQLERK RERERKMREQ QKEQREQKER ERRAEERRKE REARREVSAH 190 200 210 220 230 240 HRTMREDYSD KVKASHWSRS PPRPPRERFE LGDGRKPVKE EKMEERDLLS DLQDISDSER 250 260 270 280 290 300 KTSSAESSSA ESGSGSEEEE EEEEEEEEEG STSEESEEEE EEEEEEEEET GSNSEEASEQ 310 320 330 340 350 360 SAEEVSEEEM SEDEERENEN HLLVVPESRF DRDSGESEEA EEEVGEGTPQ SSALTEGDYV 370 380 390 400 410 420 PDSPALSPIE LKQELPKYLP ALQGCRSVEE FQCLNRIEEG TYGVVYRAKD KKTDEIVALK 430 440 450 460 470 480 RLKMEKEKEG FPITSLREIN TILKAQHPNI VTVREIVVGS NMDKIYIVMN YVEHDLKSLM 490 500 510 520 530 540 ETMKQPFLPG EVKTLMIQLL RGVKHLHDNW ILHRDLKTSN LLLSHAGILK VGDFGLAREY 550 560 570 580 590 600 GSPLKAYTPV VVTLWYRAPE LLLGAKEYST AVDMWSVGCI FGELLTQKPL FPGKSEIDQI 610 620 630 640 650 660 NKVFKDLGTP SEKIWPGYSE LPAVKKMTFS EHPYNNLRKR FGALLSDQGF DLMNKFLTYF 670 680 690 700 710 720 PGRRISAEDG LKHEYFRETP LPIDPSMFPT WPAKSEQQRV KRGTSPRPPE GGLGYSQLGD 730 740 DDLKETGFHL TTTNQGASAA GPGFSLKF
	Isoform SV11 (Alpha 2-4). Checksum: 6E088C8322ADB36A	FASTA	565	63,937
	10 20 30 40 50 60 MREDYSDKVK ASHWSRSPPR PPRERFELGD GRKPVKEEKM EERDLLSDLQ DISDSERKTS 70 80 90 100 110 120 SAESSSAESG SGSEEEEEEE EEEEEEGSTS EESEEEEEEE EEEEEETGSN SEEASEQSAE 130 140 150 160 170 180 EVSEEEMSED EERENENHLL VVPESRFDRD SGESEEAEEE VGEGTPQSSA LTEGDYVPDS 190 200 210 220 230 240 PALSPIELKQ ELPKYLPALQ GCRSVEEFQC LNRIEEGTYG VVYRAKDKKT DEIVALKRLK 250 260 270 280 290 300 MEKEKEGFPI TSLREINTIL KAQHPNIVTV REIVVGSNMD KIYIVMNYVE HDLKSLMETM 310 320 330 340 350 360 KQPFLPGEVK TLMIQLLRGV KHLHDNWILH RDLKTSNLLL SHAGILKVGD FGLAREYGSP 370 380 390 400 410 420 LKAYTPVVVT LWYRAPELLL GAKEYSTAVD MWSVGCIFGE LLTQKPLFPG KSEIDQINKV 430 440 450 460 470 480 FKDLGTPSEK IWPGYSELPA VKKMTFSEHP YNNLRKRFGA LLSDQGFDLM NKFLTYFPGR 490 500 510 520 530 540 RISAEDGLKH EYFRETPLPI DPSMFPTWPA KSEQQRVKRG TSPRPPEGGL GYSQLGDDDL 550 560 KETGFHLTTT NQGASAAGPG FSLKF
	Isoform 7 (p58). Checksum: 2008B90F6CE66EF6	FASTA	439	49,555
	10 20 30 40 50 60 MSEDEERENE NHLLVVPESR FDRDSGESEE AEEEVGEGTP QSSALTEGDY VPDSPALSPI 70 80 90 100 110 120 ELKQELPKYL PALQGCRSVE EFQCLNRIEE GTYGVVYRAK DKKTDEIVAL KRLKMEKEKE 130 140 150 160 170 180 GFPITSLREI NTILKAQHPN IVTVREIVVG SNMDKIYIVM NYVEHDLKSL METMKQPFLP 190 200 210 220 230 240 GEVKTLMIQL LRGVKHLHDN WILHRDLKTS NLLLSHAGIL KVGDFGLARE YGSPLKAYTP 250 260 270 280 290 300 VVVTLWYRAP ELLLGAKEYS TAVDMWSVGC IFGELLTQKP LFPGKSEIDQ INKVFKDLGT 310 320 330 340 350 360 PSEKIWPGYS ELPAVKKMTF SEHPYNNLRK RFGALLSDQG FDLMNKFLTY FPGRRISAED 370 380 390 400 410 420 GLKHEYFRET PLPIDPSMFP TWPAKSEQQR VKRGTSPRPP EGGLGYSQLG DDDLKETGFH 430 LTTTNQGASA AGPGFSLKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Increased expression of a 58-kDa protein kinase leads to changes in the CHO cell cycle." Bunnell B.A., Heath L.S., Adams D.E., Lahti J.M., Kidd V.J. Proc. Natl. Acad. Sci. U.S.A. 87:7467-7471(1990) [PubMed: 2217177] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 7), FUNCTION. Tissue: Liver.
[2]	Erratum Bunnell B.A., Heath L.S., Adams D.E., Lahti J.M., Kidd V.J. Proc. Natl. Acad. Sci. U.S.A. 88:2612-2612(1991) [PubMed: 2006197] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Structure and expression of the human p58clk-1 protein kinase chromosomal gene." Eipers P.G., Lahti J.M., Kidd V.J. Genomics 13:613-621(1992) [PubMed: 1639388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 7), VARIANT GLN-601. Tissue: Hematopoietic.
[4]	"Molecular cloning and expression of alternatively spliced PITSLRE protein kinase isoforms." Xiang J., Lahti J.M., Grenet J.A., Easton J.B., Kidd V.J. J. Biol. Chem. 269:15786-15794(1994) [PubMed: 8195233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SV1; 2; 3; 8 AND SV11), VARIANT CYS-57, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Cervix carcinoma.
[5]	"Duplication of a genomic region containing the Cdc2L1-2 and MMP21-22 genes on human chromosome 1p36.3 and their linkage to D1Z2." Gururajan R., Lahti J.M., Grenet J.A., Easton J., Gruber I., Ambros P.F., Kidd V.J. Genome Res. 8:929-939(1998) [PubMed: 9750192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS SV1; SV4; SV5; SV9; SV10 AND SV11), TISSUE SPECIFICITY. Tissue: Cervix carcinoma.
[6]	Govindan M.V., Warriar N. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SV11). Tissue: Placenta.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 288-795. Tissue: Uterus.
[8]	"The RNP protein, RNPS1, associates with specific isoforms of the p34cdc2-related PITSLRE protein kinases in vivo." Loyer P., Trembley J.H., Lahti J.M., Kidd V.J. J. Cell Sci. 111:1495-1506(1998) [PubMed: 9580558] [Abstract] Cited for: INTERACTION WITH RNPS1.
[9]	"Identification and characterization of a novel cell cycle-regulated internal ribosome entry site." Cornelis S., Bruynooghe Y., Denecker G., Van Huffel S., Tinton S., Beyaert R. Mol. Cell 5:597-605(2000) [PubMed: 10882096] [Abstract] Cited for: ALTERNATIVE INITIATION (ISOFORM 7), INDUCTION.
[10]	"The cyclin-dependent kinase 11(p46) isoform interacts with RanBPM." Mikolajczyk M., Shi J., Vaillancourt R.R., Sachs N.A., Nelson M. Biochem. Biophys. Res. Commun. 310:14-18(2003) [PubMed: 14511641] [Abstract] Cited for: INTERACTION WITH RANBP9, AUTOPHOSPHORYLATION, SUBCELLULAR LOCATION.
[11]	"CDK11 complexes promote pre-mRNA splicing." Hu D., Mayeda A., Trembley J.H., Lahti J.M., Kidd V.J. J. Biol. Chem. 278:8623-8629(2003) [PubMed: 12501247] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CCNL1 AND SFRS7.
[12]	"The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." Chen S., Yin X., Zhu X., Yan J., Ji S., Chen C., Cai M., Zhang S., Zong H., Hu Y., Yuan Z., Shen Z., Gu J. J. Biol. Chem. 278:20029-20036(2003) [PubMed: 12624090] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAK1.
[13]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283; SER-589 AND THR-595, MASS SPECTROMETRY. Tissue: Epithelium.
[14]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-595, MASS SPECTROMETRY. Tissue: Epithelium.
[15]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283 AND THR-595, MASS SPECTROMETRY. Tissue: Epithelium.
[16]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-595, MASS SPECTROMETRY.
[17]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-381; SER-384; THR-395; SER-410; SER-414; TYR-594 AND THR-595, MASS SPECTROMETRY.
[18]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] CYS-57; TRP-201; LEU-414; ALA-452; VAL-463; SER-506; GLN-601; ASN-641 AND VAL-670.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M37712 mRNA. Translation: AAA36406.1. Sequence problems. M88563 , M88553, M88554, M88555, M88558, M88559, M88560, M88561, M88562 Genomic DNA. Translation: AAB59449.1. Sequence problems. U04815 mRNA. Translation: AAA19581.1. U04816 mRNA. Translation: AAA19582.1. U04817 mRNA. Translation: AAA19583.1. U04818 mRNA. Translation: AAA19584.1. U04824 mRNA. Translation: AAA19586.1. AF067512 mRNA. Translation: AAC72077.1. AF067513 mRNA. Translation: AAC72078.1. AF067514 mRNA. Translation: AAC72079.1. AF067515 mRNA. Translation: AAC72080.1. AF067516 mRNA. Translation: AAC72081.1. AF067517 mRNA. Translation: AAC72082.1. AF080683 , AF080685, AF080686, AF080687, AF080688, AF092429, AF092430, AF080678, AF080679, AF080680, AF080681, AF080682 Genomic DNA. Translation: AAC83662.1. AF080683 , AF080685, AF080686, AF080687, AF080688, AF092429, AF092430, AF080678, AF080679, AF080680, AF080681, AF080682 Genomic DNA. Translation: AAC83663.1. AF080683 , AF080685, AF080686, AF080687, AF080688, AF092429, AF092430, AF080678, AF080679, AF080680, AF080681, AF080682 Genomic DNA. Translation: AAC83664.1. Sequence problems. AF080683 , AF080685, AF080686, AF080687, AF080688, AF092429, AF092430, AF080678, AF080679, AF080680, AF080681, AF080682 Genomic DNA. Translation: AAC83665.1. AF080683 , AF092430, AF080678, AF080679, AF080680, AF080681, AF080682 Genomic DNA. Translation: AAC83666.1. AF174497 mRNA. Translation: AAF36538.1. Different initiation. BC033069 mRNA. Translation: AAH33069.1. Different initiation.
IPI	IPI00071185. IPI00071188. IPI00215999. IPI00337617. IPI00376979. IPI00376980. IPI00376983. IPI00395687. IPI00736011. IPI00759690.
PIR	A38282. B54024. E54024. F54024. H54024. T09568.
RefSeq	NP_277021.1. NP_277022.1. NP_277023.1. NP_277024.1. NP_277027.1. NP_277028.1.
UniGene	Hs.651228 Hs.709182
3D structure databases
HSSP	HSSP built from PDB template 1H00 based on UniProtKB P24941.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:29015N.
IntAct	P21127. 4 interactions.
PTM databases
PhosphoSite	P21127.
Proteomic databases
PRIDE	P21127.
Genome annotation databases
Ensembl	ENSG00000008128. Homo sapiens. [Contig view]
GeneID	984.
KEGG	hsa:984.
Organism-specific databases
GeneCards	GC01M001626. GC01M001627.
H-InvDB	HIX0000039.
HGNC	HGNC:1729. CDC2L1.
HPA	CAB010467.
MIM	176873. gene.
PharmGKB	PA26262.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P21127.
Enzyme and pathway databases
BRENDA	2.7.11.22. 247.
Pathway_Interaction_DB	ar_pathway. Coregulation of Androgen receptor activity.
Gene expression databases
Bgee	P21127.
GermOnline	ENSG00000008128. Homo sapiens.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	4128.
PMAP-CutDB	P21127.
SOURCE	Search...

Entry information

Entry name

CD2L1_HUMAN

Accession

Primary (citable) accession number: P21127
Secondary accession number(s): O95265 , Q12817, Q12818, Q12819, Q12820, Q12822, Q8N530, Q9NZS5, Q9UBJ0, Q9UBQ1, Q9UBR0, Q9UNY2, Q9UP57, Q9UP58, Q9UP59

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	September 19, 2003
Last modified:	June 16, 2009
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)