P21115 (B5_VACCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Plaque-size/host range protein

Alternative name(s):
Protein B5

Gene names

Name:	PS/HR
ORF Names:	B5R

Organism

Vaccinia virus (strain Copenhagen) (VACV) [Complete proteome]

Taxonomic identifier

10249 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Play a role in the dissolution of the outermost membrane of extracellular enveloped virions (EEV) to allow virion entry into host cells. Participates also in wrapping intracellular mature virions (IMV) to form intracellular enveloped virions (IEV) By similarity.
Subunit structure	Interacts with A33; this interaction is required for efficient targeting of A33 and B5 into enveloped virions. Interacts with A34; this interaction is required for the correct glycosylation, trafficking and stability of A34 and B5 incorporation into extracellular enveloped virions By similarity.
Subcellular location	Host membrane; Single-pass type I membrane protein Potential. Virion. Note: B5 is found on extracellular virions but not on intracellular mature virions By similarity.
Sequence similarities	Belongs to the receptors of complement activation (RCA) family. Contains 4 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Cellular component	Host membrane Membrane Virion
Domain	Repeat Signal Sushi Transmembrane Transmembrane helix
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of complement activation Inferred from electronic annotation. Source: InterPro
Cellular component	host cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	complement binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 317	300	Plaque-size/host range protein		PRO_0000006017
Regions
	Topological domain	18 – 279	262	Extracellular Potential
	Transmembrane	280 – 300	21	Helical; Potential
	Topological domain	301 – 317	17	Cytoplasmic Potential
	Domain	19 – 74	56	Sushi 1
	Domain	75 – 126	52	Sushi 2
	Domain	127 – 184	58	Sushi 3
	Domain	185 – 239	55	Sushi 4
Amino acid modifications
	Disulfide bond	21 ↔ 61		By similarity
	Disulfide bond	48 ↔ 72		By similarity
	Disulfide bond	76 ↔ 110		By similarity
	Disulfide bond	101 ↔ 125		By similarity
	Disulfide bond	130 ↔ 171		By similarity
	Disulfide bond	157 ↔ 182		By similarity
	Disulfide bond	186 ↔ 225		By similarity
	Disulfide bond	211 ↔ 237		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21115 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: B66E3E29EF3C2BA7	FASTA	317	35,109
	10 20 30 40 50 60 MKTISVVTLL CVLPAVVYST CTVPTMNNAK LTSTETSFNN NQKVTFTCDQ GYHSSDPNAV 70 80 90 100 110 120 CETDKWKYEN PCKKMCTVSD YISELYNKPL YEVNSTMTLS CNGETKYFRC EEKNGNTSWN 130 140 150 160 170 180 DTVTCPNAEC QPLQLEHGSC QPVKEKYSFG EYMTINCDVG YEVIGASYIS CTANSWNVIP 190 200 210 220 230 240 SCQQKCDIPS LSNGLISGST FSIGGVIHLS CKSGFILTGS PSSTCIDGKW NPVLPICVRT 250 260 270 280 290 300 NEEFDPVDDG PDDETDLSKL SKDVVQYEQE IESLEATYHI IIVALTIMGV IFLISVIVLV 310 CSCDKNNDQY KFHKLLP

References

[1]	"The complete DNA sequence of vaccinia virus." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:247-266(1990) [PubMed: 2219722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Appendix to 'The complete DNA sequence of vaccinia virus'." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:517-563(1990) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35027 Genomic DNA. Translation: AAA48201.1.
PIR	D42526.
3D structure databases
ProteinModelPortal	P21115.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011176. CIP_VAC_C3L. IPR016060. Complement_control_module. IPR000436. Sushi_SCR_CCP. [Graphical view]
Gene3D	G3DSA:2.10.70.10. Complement_control_module. 3 hits.
Pfam	PF00084. Sushi. 3 hits. [Graphical view]
PIRSF	PIRSF002486. CIP_VAC_C3L. 1 hit.
SMART	SM00032. CCP. 4 hits. [Graphical view]
SUPFAM	SSF57535. Complement_control_module. 3 hits.
PROSITE	PS50923. SUSHI. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B5_VACCC

Accession

Primary (citable) accession number: P21115

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	October 19, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program