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P21112 (MCRZ_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-coenzyme M reductase II subunit gamma

Short name=MCR II gamma
EC=2.8.4.1
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase gamma
Gene names
Name:mrtG
Ordered Locus Names:MTH_1130
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activity

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactor

Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.

Pathway

One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.

Subunit structure

Hexamer of two alpha, two beta, and two gamma chains.

Developmental stage

There are two MCR complexes in this bacteria. MCR II is expressed in the early growth phase. Late growth cells contains mostly MCR I.

Ontologies

Keywords
   Biological processMethanogenesis
   Molecular functionTransferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processmethanogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncoenzyme-B sulfoethylthiotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 265264Methyl-coenzyme M reductase II subunit gamma
PRO_0000147480

Experimental info

Sequence conflict1321D → V in AAA73438. Ref.1
Sequence conflict2301D → DD in AAA73438. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P21112 [UniParc].

Last modified January 23, 2007. Version 5.
Checksum: F1E6783AAE7A3CA8

FASTA26530,567
        10         20         30         40         50         60 
MTYKAQYTPG ETQIAENRRK HMDPDYEFRK LREVSDEDLV KVLGHRNPGE SYKSVHPPLD 

        70         80         90        100        110        120 
EMDFEEDIVR DMVEPIQGAK EGVRVRYIQF ADSMYNAPAQ PYDRARTYMW RYRGVDTGTL 

       130        140        150        160        170        180 
SGRQVIEMRE LDLEGVSKEL VETELFDPAT TGIRGATVHG HSLRLDENGL MFDALQRYVF 

       190        200        210        220        230        240 
DEEKGHVVYV KDQVGRPLDE PVDMGQPLGE DELKKITTIY RKDNIAMRDD KEAIEVVENI 

       250        260 
HTGRTMGGFG MDVFKDDLRK RLGDD 

« Hide

References

« Hide 'large scale' references
[1]"Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H."
Pihl T.D., Sharma S., Reeve J.N.
J. Bacteriol. 176:6384-6391(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
[2]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
[3]"Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta H."
Rospert S., Linder D., Ellermann J., Thauer R.K.
Eur. J. Biochem. 194:871-877(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-16.
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09990 Genomic DNA. Translation: AAA73438.1.
AE000666 Genomic DNA. Translation: AAB85619.1.
PIRT45149.
RefSeqNP_276258.1. NC_000916.1.

3D structure databases

ProteinModelPortalP21112.
SMRP21112. Positions 5-251.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP21112. 1 interaction.
STRING187420.MTH1130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB85619; AAB85619; MTH_1130.
GeneID1471538.
KEGGmth:MTH1130.

Phylogenomic databases

eggNOGCOG4057.
KOK00402.
OMAGHRNPGE.
ProtClustDBCLSK876060.

Enzyme and pathway databases

BioCycMetaCyc:MRTGMAUTO-MONOMER.
MTHE187420:GJNM-1132-MONOMER.
UniPathwayUPA00646; UER00699.

Family and domain databases

Gene3D3.90.320.20. 1 hit.
InterProIPR009024. Me_CoM_Rdtase_Fd-like_fold.
IPR003178. Me_CoM_Rdtase_gsu.
[Graphical view]
PfamPF02240. MCR_gamma. 1 hit.
[Graphical view]
PIRSFPIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDomPD005845. Me_CoM_Rdtase_gsu. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF55088. SSF55088. 1 hit.
TIGRFAMsTIGR03259. met_CoM_red_gam. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMCRZ_METTH
AccessionPrimary (citable) accession number: P21112
Secondary accession number(s): O27202, Q50486
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 99 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways