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Protein

Methyl-coenzyme M reductase II subunit alpha

Gene

mrtA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activityi

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactori

coenzyme F430By similarityNote: Binds 1 coenzyme F430 noncovalently per subunit. Coenzyme F430 is a yellow nickel porphinoid.By similarity

Pathwayi: methyl-coenzyme M reduction

This protein is involved in step 1 of the subpathway that synthesizes methane from methyl-coenzyme M.
Proteins known to be involved in this subpathway in this organism are:
  1. Methyl-coenzyme M reductase subunit alpha (mcrA), Methyl-coenzyme M reductase I subunit gamma (mcrG), Methyl-coenzyme M reductase II subunit gamma (mrtG), Methyl-coenzyme M reductase I subunit beta (mcrB), Methyl-coenzyme M reductase II subunit beta (mrtB), Methyl-coenzyme M reductase II subunit alpha (mrtA)
This subpathway is part of the pathway methyl-coenzyme M reduction, which is itself part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes methane from methyl-coenzyme M, the pathway methyl-coenzyme M reduction and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi150NickelBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processMethanogenesis
LigandMetal-binding, Nickel

Enzyme and pathway databases

BioCyciMetaCyc:MRTAMAUTO-MONOMER
MTHE187420:G1G16-1090-MONOMER
UniPathwayiUPA00646; UER00699

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-coenzyme M reductase II subunit alpha (EC:2.8.4.1)
Short name:
MCR II alpha
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase alpha
Gene namesi
Name:mrtA
Ordered Locus Names:MTH_1129
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001474571 – 553Methyl-coenzyme M reductase II subunit alphaAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei260Pros-methylhistidineBy similarity1
Modified residuei2745-methylarginineBy similarity1

Keywords - PTMi

Methylation

Expressioni

Developmental stagei

There are two MCR complexes in this bacteria. MCR II is expressed in the early growth phase. Late growth cells contains mostly MCR I.

Interactioni

Subunit structurei

Hexamer of two alpha, two beta, and two gamma chains.

Protein-protein interaction databases

IntActiP21110, 2 interactors
STRINGi187420.MTH1129

Structurei

3D structure databases

ProteinModelPortaliP21110
SMRiP21110
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiarCOG04857 Archaea
COG4058 LUCA
KOiK00399
OMAiVGHQPEY
OrthoDBiPOG093Z00ZI

Family and domain databases

Gene3Di1.20.840.10, 1 hit
3.30.70.470, 1 hit
3.90.390.10, 1 hit
InterProiView protein in InterPro
IPR016212 Me_CoM_Rdtase_asu
IPR008924 Me_CoM_Rdtase_asu/bsu_C
IPR009047 Me_CoM_Rdtase_asu_C
IPR003183 Me_CoM_Rdtase_asu_N
IPR015811 Me_CoM_Rdtase_asu_N_sub1
IPR015823 Me_CoM_Rdtase_asu_N_sub2
IPR009024 Me_CoM_Rdtase_Fd-like_fold
PfamiView protein in Pfam
PF02249 MCR_alpha, 1 hit
PF02745 MCR_alpha_N, 1 hit
PIRSFiPIRSF000262 MCR_alpha, 1 hit
SUPFAMiSSF48081 SSF48081, 1 hit
SSF55088 SSF55088, 1 hit
TIGRFAMsiTIGR03256 met_CoM_red_alp, 1 hit

Sequencei

Sequence statusi: Complete.

P21110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEKKLFLTA LKKKFEVEDP DEKYTNFYCF GGWEQSARKK EFTEYAKKAA
60 70 80 90 100
EKRGGIPFYN PDIGVPLGQR KLMAYRVSGT DAYVEGDDLH FVNNAAIQQM
110 120 130 140 150
VDDIKRTVIV GMDTAHAVLE KRLGVEVTPE TINEYMEAIN HALPGGAVVQ
160 170 180 190 200
EHMVEVHPGL VEDCYAKIFT GDDNLADELD KRILIDINKE FPEEQAEQLK
210 220 230 240 250
SYIGNRTYQV NRVPTIVVRT CDGGTVSRWS AMQIGMSFIS AYKLCAGEAA
260 270 280 290 300
IADFSYAAKH ADVIEMGTIM PARRARGPNE PGGVAFGTFA DIVQASRVSD
310 320 330 340 350
DPAKISLEVI AGAAALYDQV WLGSYMSGGV GFTQYATAAY TDDILDDFVY
360 370 380 390 400
YGMEYVDDKY GICGTKPTMD VVRDISTEVT LYSLEQYEEY PTLLEDHFGG
410 420 430 440 450
SQRAAVAAAA AGCSTAFATG NSNAGINGWY LSQILHKEAH SRLGFYGYDL
460 470 480 490 500
QDQCGASNSL SIRSDEGLIH ELRGPNYPNY AMNVGHQPEY AGIAQAPHAA
510 520 530 540 550
RGDAFCTNPL IKVAFADKDL SFDFTSPRKS IAAGALREFM PEGERDLIIP

AGK
Length:553
Mass (Da):60,707
Last modified:May 30, 2000 - v3
Checksum:i8A3511715F7EFE00
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6L → H in AAA73439 (PubMed:7929010).Curated1
Sequence conflicti24Y → I in AAA73439 (PubMed:7929010).Curated1
Sequence conflicti168I → V in AAA73439 (PubMed:7929010).Curated1
Sequence conflicti200K → KEQLK (PubMed:7929010).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09990 Genomic DNA Translation: AAA73439.1
AE000666 Genomic DNA Translation: AAB85618.1
PIRiC69017
T45150
RefSeqiWP_010876753.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaiAAB85618; AAB85618; MTH_1129
GeneIDi1471537
KEGGimth:MTH_1129
PATRICifig|187420.15.peg.1106

Similar proteinsi

Entry informationi

Entry nameiMCRX_METTH
AccessioniPrimary (citable) accession number: P21110
Secondary accession number(s): O27201, Q50487
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 30, 2000
Last modified: March 28, 2018
This is version 122 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health