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Protein

Tropomyosin alpha-3 chain

Gene

Tpm3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-MMU-390522. Striated Muscle Contraction.
R-MMU-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin alpha-3 chain
Alternative name(s):
Gamma-tropomyosin
Tropomyosin-3
Gene namesi
Name:Tpm3
Synonyms:Tpm-5, Tpm5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1890149. Tpm3.

Subcellular locationi

GO - Cellular componenti

  • cortical cytoskeleton Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • growth cone Source: MGI
  • neuron projection Source: MGI
  • podosome Source: MGI
  • stress fiber Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 285284Tropomyosin alpha-3 chainPRO_0000205633Add
BLAST
Isoform 2 (identifier: P21107-2)
Initiator methionineiRemovedBy similarity

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylmethionineBy similarity
Modified residuei54 – 541PhosphothreonineBy similarity
Modified residuei62 – 621PhosphoserineBy similarity
Cross-linki78 – 78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei88 – 881PhosphoserineBy similarity
Modified residuei109 – 1091PhosphothreonineBy similarity
Modified residuei207 – 2071PhosphoserineBy similarity
Modified residuei216 – 2161PhosphoserineBy similarity
Modified residuei253 – 2531PhosphothreonineBy similarity
Modified residuei262 – 2621PhosphotyrosineBy similarity
Modified residuei272 – 2721PhosphoserineCombined sources
Modified residuei283 – 2831PhosphothreonineBy similarity
Modified residuei284 – 2841PhosphoserineCombined sources
Isoform 2 (identifier: P21107-2)
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei228 – 2281N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP21107.
MaxQBiP21107.
PeptideAtlasiP21107.
PRIDEiP21107.

2D gel databases

COMPLUYEAST-2DPAGEP21107.
REPRODUCTION-2DPAGEP21107.
SWISS-2DPAGEP21107.

PTM databases

iPTMnetiP21107.
PhosphoSiteiP21107.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027940.
CleanExiMM_TPM3.
ExpressionAtlasiP21107. baseline and differential.
GenevisibleiP21107. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. Binds to TMOD1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi208522. 5 interactions.
DIPiDIP-32061N.
IntActiP21107. 4 interactions.
MINTiMINT-1868808.

Structurei

3D structure databases

ProteinModelPortaliP21107.
SMRiP21107. Positions 9-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 285285By similarityAdd
BLAST

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231521.
HOVERGENiHBG107404.
InParanoidiP21107.
KOiK09290.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P21107-1) [UniParc]FASTAAdd to basket
Also known as: Skeletal muscle

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMEAIKKKMQ MLKLDKENVL DRAEQAEAEQ KQAEERSKQL EDELATMQKK
60 70 80 90 100
LKGTEDELDK YSEALKDAQE KLELAEKKAA DAEAEVASLN RRIQLVEEEL
110 120 130 140 150
DRAQERLATA LQKLEEAEKA ADESERGMKV IENRALKDEE KMELQEIQLK
160 170 180 190 200
EAKHIAEEAD RKYEEVARKL VIIEGDLERT EERAELAESK CSELEEELKN
210 220 230 240 250
VTNNLKSLEA QAEKYSQKED KYEEEIKILT DKLKEAETRA EFAERSVAKL
260 270 280
EKTIDDLEDE LYAQKLKYKA ISDELDHALN DMTSI
Length:285
Mass (Da):32,994
Last modified:June 26, 2013 - v3
Checksum:iF4407C132303002C
GO
Isoform 2 (identifier: P21107-2) [UniParc]FASTAAdd to basket
Also known as: Cytoskeletal

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: MMEAIKKKMQ...LELAEKKAAD → MAGTTTIEAV...VEGERRAREQ
     190-212: KCSELEEELKNVTNNLKSLEAQA → RCREMDEQIRLMDQNLKCLSAAE
     260-285: ELYAQKLKYKAISDELDHALNDMTSI → KLKCTKEEHLCTQRMLDQTLLDLNEM

Show »
Length:248
Mass (Da):29,021
Checksum:i90F49312A655F53D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191V → F in AAA03725 (Ref. 2) Curated
Sequence conflicti112 – 1121Q → R in AAA03725 (Ref. 2) Curated
Sequence conflicti181 – 1811E → G in AAA03725 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8181MMEAI…KKAAD → MAGTTTIEAVKRKIQVLQQQ ADDAEERAERLQREVEGERR AREQ in isoform 2. 2 PublicationsVSP_006608Add
BLAST
Alternative sequencei190 – 21223KCSEL…LEAQA → RCREMDEQIRLMDQNLKCLS AAE in isoform 2. 2 PublicationsVSP_006609Add
BLAST
Alternative sequencei260 – 28526ELYAQ…DMTSI → KLKCTKEEHLCTQRMLDQTL LDLNEM in isoform 2. 2 PublicationsVSP_006610Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53753 mRNA. Translation: CAA37782.1.
U04541 mRNA. Translation: AAA03725.1.
AF317223 mRNA. Translation: AAG38596.1.
AK088111 mRNA. Translation: BAC40150.1.
AK088902 mRNA. Translation: BAC40643.1.
X72633 mRNA. Translation: CAA51209.1.
CCDSiCCDS57223.1. [P21107-2]
PIRiI48852.
S11390.
RefSeqiNP_001240667.1. NM_001253738.1. [P21107-2]
NP_001280677.2. NM_001293748.1. [P21107-1]
UniGeneiMm.240839.
Mm.421791.
Mm.490309.

Genome annotation databases

EnsembliENSMUST00000029549; ENSMUSP00000029549; ENSMUSG00000027940. [P21107-2]
GeneIDi59069.
KEGGimmu:59069.
UCSCiuc008qbf.3. mouse. [P21107-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53753 mRNA. Translation: CAA37782.1.
U04541 mRNA. Translation: AAA03725.1.
AF317223 mRNA. Translation: AAG38596.1.
AK088111 mRNA. Translation: BAC40150.1.
AK088902 mRNA. Translation: BAC40643.1.
X72633 mRNA. Translation: CAA51209.1.
CCDSiCCDS57223.1. [P21107-2]
PIRiI48852.
S11390.
RefSeqiNP_001240667.1. NM_001253738.1. [P21107-2]
NP_001280677.2. NM_001293748.1. [P21107-1]
UniGeneiMm.240839.
Mm.421791.
Mm.490309.

3D structure databases

ProteinModelPortaliP21107.
SMRiP21107. Positions 9-285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208522. 5 interactions.
DIPiDIP-32061N.
IntActiP21107. 4 interactions.
MINTiMINT-1868808.

PTM databases

iPTMnetiP21107.
PhosphoSiteiP21107.

2D gel databases

COMPLUYEAST-2DPAGEP21107.
REPRODUCTION-2DPAGEP21107.
SWISS-2DPAGEP21107.

Proteomic databases

EPDiP21107.
MaxQBiP21107.
PeptideAtlasiP21107.
PRIDEiP21107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029549; ENSMUSP00000029549; ENSMUSG00000027940. [P21107-2]
GeneIDi59069.
KEGGimmu:59069.
UCSCiuc008qbf.3. mouse. [P21107-1]

Organism-specific databases

CTDi7170.
MGIiMGI:1890149. Tpm3.

Phylogenomic databases

GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231521.
HOVERGENiHBG107404.
InParanoidiP21107.
KOiK09290.

Enzyme and pathway databases

ReactomeiR-MMU-390522. Striated Muscle Contraction.
R-MMU-445355. Smooth Muscle Contraction.

Miscellaneous databases

PROiP21107.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027940.
CleanExiMM_TPM3.
ExpressionAtlasiP21107. baseline and differential.
GenevisibleiP21107. MM.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPM3_MOUSE
AccessioniPrimary (citable) accession number: P21107
Secondary accession number(s): Q09021
, Q60606, Q80SW6, Q9EPW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 26, 2013
Last modified: September 7, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.