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UniProtKB - P21107 (TPM3_MOUSE)

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Protein

Tropomyosin alpha-3 chain

Gene

Tpm3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionActin-binding, Muscle protein

Enzyme and pathway databases

ReactomeiR-MMU-390522. Striated Muscle Contraction.
R-MMU-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin alpha-3 chain
Alternative name(s):
Gamma-tropomyosin
Tropomyosin-3
Gene namesi
Name:Tpm3
Synonyms:Tpm-5, Tpm5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1890149. Tpm3.

Subcellular locationi

Isoform 2 :

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cortical cytoskeleton Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • growth cone Source: MGI
  • muscle thin filament tropomyosin Source: GO_Central
  • neuron projection Source: MGI
  • podosome Source: MGI
  • stress fiber Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002056332 – 285Tropomyosin alpha-3 chainAdd BLAST284
Isoform 2 (identifier: P21107-2)
Initiator methionineiRemovedBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylmethionineBy similarity1
Modified residuei54PhosphothreonineBy similarity1
Modified residuei62PhosphoserineBy similarity1
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei88PhosphoserineBy similarity1
Modified residuei109PhosphothreonineBy similarity1
Modified residuei207PhosphoserineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei253PhosphothreonineBy similarity1
Modified residuei262PhosphotyrosineBy similarity1
Modified residuei272PhosphoserineCombined sources1
Modified residuei283PhosphothreonineBy similarity1
Modified residuei284PhosphoserineCombined sources1
Isoform 2 (identifier: P21107-2)
Modified residuei2N-acetylalanineBy similarity1
Modified residuei228N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP21107.
MaxQBiP21107.
PeptideAtlasiP21107.
PRIDEiP21107.

2D gel databases

COMPLUYEAST-2DPAGEiP21107.
REPRODUCTION-2DPAGEiP21107.
SWISS-2DPAGEiP21107.

PTM databases

iPTMnetiP21107.
PhosphoSitePlusiP21107.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027940.
CleanExiMM_TPM3.
ExpressionAtlasiP21107. baseline and differential.
GenevisibleiP21107. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. Binds to TMOD1 (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Protein-protein interaction databases

BioGridi208522. 5 interactors.
DIPiDIP-32061N.
IntActiP21107. 5 interactors.
MINTiMINT-1868808.

Structurei

3D structure databases

ProteinModelPortaliP21107.
SMRiP21107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 285By similarityAdd BLAST285

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231521.
HOVERGENiHBG107404.
InParanoidiP21107.
KOiK09290.

Family and domain databases

InterProiView protein in InterPro
IPR000533. Tropomyosin.
PfamiView protein in Pfam
PF00261. Tropomyosin. 1 hit.
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiView protein in PROSITE
PS00326. TROPOMYOSIN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P21107-1) [UniParc]FASTAAdd to basket
Also known as: Skeletal muscle

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMEAIKKKMQ MLKLDKENVL DRAEQAEAEQ KQAEERSKQL EDELATMQKK 
        60         70         80         90        100
LKGTEDELDK YSEALKDAQE KLELAEKKAA DAEAEVASLN RRIQLVEEEL 
       110        120        130        140        150
DRAQERLATA LQKLEEAEKA ADESERGMKV IENRALKDEE KMELQEIQLK 
       160        170        180        190        200
EAKHIAEEAD RKYEEVARKL VIIEGDLERT EERAELAESK CSELEEELKN 
       210        220        230        240        250
VTNNLKSLEA QAEKYSQKED KYEEEIKILT DKLKEAETRA EFAERSVAKL 
       260        270        280 
EKTIDDLEDE LYAQKLKYKA ISDELDHALN DMTSI
Length:285
Mass (Da):32,994
Last modified:June 26, 2013 - v3
Checksum:iF4407C132303002C
GO
Isoform 2 (identifier: P21107-2) [UniParc]FASTAAdd to basket
Also known as: Cytoskeletal

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: MMEAIKKKMQ...LELAEKKAAD → MAGTTTIEAV...VEGERRAREQ
     190-212: KCSELEEELKNVTNNLKSLEAQA → RCREMDEQIRLMDQNLKCLSAAE
     260-285: ELYAQKLKYKAISDELDHALNDMTSI → KLKCTKEEHLCTQRMLDQTLLDLNEM

Show »
Length:248
Mass (Da):29,021
Checksum:i90F49312A655F53D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19V → F in AAA03725 (Ref. 2) Curated1
Sequence conflicti112Q → R in AAA03725 (Ref. 2) Curated1
Sequence conflicti181E → G in AAA03725 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0066081 – 81MMEAI…KKAAD → MAGTTTIEAVKRKIQVLQQQ ADDAEERAERLQREVEGERR AREQ in isoform 2. 2 PublicationsAdd BLAST81
Alternative sequenceiVSP_006609190 – 212KCSEL…LEAQA → RCREMDEQIRLMDQNLKCLS AAE in isoform 2. 2 PublicationsAdd BLAST23
Alternative sequenceiVSP_006610260 – 285ELYAQ…DMTSI → KLKCTKEEHLCTQRMLDQTL LDLNEM in isoform 2. 2 PublicationsAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53753 mRNA. Translation: CAA37782.1.
U04541 mRNA. Translation: AAA03725.1.
AF317223 mRNA. Translation: AAG38596.1.
AK088111 mRNA. Translation: BAC40150.1.
AK088902 mRNA. Translation: BAC40643.1.
X72633 mRNA. Translation: CAA51209.1.
CCDSiCCDS57223.1. [P21107-2]
PIRiI48852.
S11390.
RefSeqiNP_001240667.1. NM_001253738.1. [P21107-2]
NP_001280677.2. NM_001293748.1. [P21107-1]
UniGeneiMm.240839.
Mm.421791.
Mm.442249.
Mm.490309.

Genome annotation databases

EnsembliENSMUST00000029549; ENSMUSP00000029549; ENSMUSG00000027940. [P21107-2]
GeneIDi59069.
KEGGimmu:59069.
UCSCiuc008qbf.3. mouse. [P21107-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTPM3_MOUSE
AccessioniPrimary (citable) accession number: P21107
Secondary accession number(s): Q09021
, Q60606, Q80SW6, Q9EPW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 26, 2013
Last modified: May 10, 2017
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families