Tpm3

Functionⁱ

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

GO - Molecular functionⁱ

actin filament binding Source: GO_Central
structural constituent of muscle Source: GO_Central

Complete GO annotation...

GO - Biological processⁱ

actin filament organization Source: GO_Central
muscle contraction Source: GO_Central

Complete GO annotation...

Keywordsⁱ

Molecular function	Actin-binding, Muscle protein

Molecular function

Actin-binding, Muscle protein

Enzyme and pathway databases

Reactomeⁱ	R-MMU-390522. Striated Muscle Contraction. R-MMU-445355. Smooth Muscle Contraction.

Reactomeⁱ

R-MMU-390522. Striated Muscle Contraction.
R-MMU-445355. Smooth Muscle Contraction.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tropomyosin alpha-3 chain Alternative name(s): Gamma-tropomyosin Tropomyosin-3
Gene namesⁱ	Name:Tpm3 Synonyms:Tpm-5, Tpm5
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 3

Organism-specific databases

MGIⁱ	MGI:1890149. Tpm3.

Subcellular locationⁱ

Isoform 2 :

Cytoplasm › cytoskeleton

GO - Cellular componentⁱ

actin cytoskeleton Source: MGI
cortical cytoskeleton
Source: MGI
Inferred from direct assayⁱ
- 16765662
- 9473354
cytoplasm
Source: MGI
Inferred from direct assayⁱ
- 16765662
cytosol Source: MGI
extracellular exosome Source: MGI
growth cone
Source: MGI
Inferred from direct assayⁱ
- 15888546
muscle thin filament tropomyosin Source: GO_Central
neuron projection
Source: MGI
Inferred from direct assayⁱ
- 15888546
podosome
Source: MGI
Inferred from direct assayⁱ
- 16765662
stress fiber
Source: MGI
Inferred from sequence orthologyⁱ
- 16236705

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000205633	2 – 285	Tropomyosin alpha-3 chainAdd BLAST	284
Isoform 2 (identifier: P21107-2)
Initiator methionineⁱ		RemovedBy similarity

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylmethionineBy similarity	1
Modified residueⁱ	54	PhosphothreonineBy similarity	1
Modified residueⁱ	62	PhosphoserineBy similarity	1
Cross-linkⁱ	78	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	88	PhosphoserineBy similarity	1
Modified residueⁱ	109	PhosphothreonineBy similarity	1
Modified residueⁱ	207	PhosphoserineBy similarity	1
Modified residueⁱ	216	PhosphoserineBy similarity	1
Modified residueⁱ	253	PhosphothreonineBy similarity	1
Modified residueⁱ	262	PhosphotyrosineBy similarity	1
Modified residueⁱ	272	PhosphoserineCombined sources	1
Modified residueⁱ	283	PhosphothreonineBy similarity	1
Modified residueⁱ	284	PhosphoserineCombined sources	1
Isoform 2 (identifier: P21107-2)
Modified residueⁱ	2	N-acetylalanineBy similarity	1
Modified residueⁱ	228	N6-acetyllysineBy similarity	1

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	P21107.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P21107.
PeptideAtlas More...PeptideAtlasⁱ	P21107.
PRIDEⁱ	P21107.

2D gel databases

COMPLUYEAST-2DPAGEⁱ	P21107.
REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	P21107.
SWISS-2DPAGEⁱ	P21107.

PTM databases

iPTMnetⁱ	P21107.
PhosphoSitePlusⁱ	P21107.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000027940.
CleanExⁱ	MM_TPM3.
ExpressionAtlasⁱ	P21107. baseline and differential.
Genevisibleⁱ	P21107. MM.

Interactionⁱ

Subunit structureⁱ

Heterodimer of an alpha and a beta chain. Binds to TMOD1 (By similarity).By similarity

GO - Molecular functionⁱ

actin filament binding Source: GO_Central

Complete GO annotation...

Protein-protein interaction databases

BioGridⁱ	208522. 5 interactors.
Database of interacting proteins More...DIPⁱ	DIP-32061N.
IntActⁱ	P21107. 5 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1868808.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P21107.
SMRⁱ	P21107.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	1 – 285	By similarityAdd BLAST		285

Domainⁱ

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesⁱ

Belongs to the tropomyosin family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074494.
HOGENOMⁱ	HOG000231521.
HOVERGENⁱ	HBG107404.
InParanoidⁱ	P21107.
KEGG Orthology (KO) More...KOⁱ	K09290.

Family and domain databases

InterProⁱ	View protein in InterPro IPR000533. Tropomyosin.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00261. Tropomyosin. 1 hit.
PRINTSⁱ	PR00194. TROPOMYOSIN.
PROSITEⁱ	View protein in PROSITE PS00326. TROPOMYOSIN. 1 hit.

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P21107-1) [UniParc]FASTA Add to basket

Also known as: Skeletal muscle

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMEAIKKKMQ MLKLDKENVL DRAEQAEAEQ KQAEERSKQL EDELATMQKK 
        60         70         80         90        100
LKGTEDELDK YSEALKDAQE KLELAEKKAA DAEAEVASLN RRIQLVEEEL 
       110        120        130        140        150
DRAQERLATA LQKLEEAEKA ADESERGMKV IENRALKDEE KMELQEIQLK 
       160        170        180        190        200
EAKHIAEEAD RKYEEVARKL VIIEGDLERT EERAELAESK CSELEEELKN 
       210        220        230        240        250
VTNNLKSLEA QAEKYSQKED KYEEEIKILT DKLKEAETRA EFAERSVAKL 
       260        270        280 
EKTIDDLEDE LYAQKLKYKA ISDELDHALN DMTSI

Length:285

Mass (Da):32,994

Last modified:June 26, 2013 - v3

Checksum:ⁱF4407C132303002C

GO

Isoform 2 (identifier: P21107-2) [UniParc]FASTA Add to basket

Also known as: Cytoskeletal

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: MMEAIKKKMQ...LELAEKKAAD → MAGTTTIEAV...VEGERRAREQ
     190-212: KCSELEEELKNVTNNLKSLEAQA → RCREMDEQIRLMDQNLKCLSAAE
     260-285: ELYAQKLKYKAISDELDHALNDMTSI → KLKCTKEEHLCTQRMLDQTLLDLNEM

« Hide

        10         20         30         40         50
MAGTTTIEAV KRKIQVLQQQ ADDAEERAER LQREVEGERR AREQAEAEVA 
        60         70         80         90        100
SLNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRALK 
       110        120        130        140        150
DEEKMELQEI QLKEAKHIAE EADRKYEEVA RKLVIIEGDL ERTEERAELA 
       160        170        180        190        200
ESRCREMDEQ IRLMDQNLKC LSAAEEKYSQ KEDKYEEEIK ILTDKLKEAE 
       210        220        230        240 
TRAEFAERSV AKLEKTIDDL EDKLKCTKEE HLCTQRMLDQ TLLDLNEM

Show »

Length:248

Mass (Da):29,021

Checksum:ⁱ90F49312A655F53D

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	19	V → F in AAA03725 (Ref. 2) Curated	1
Sequence conflictⁱ	112	Q → R in AAA03725 (Ref. 2) Curated	1
Sequence conflictⁱ	181	E → G in AAA03725 (Ref. 2) Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_006608	1 – 81	MMEAI…KKAAD → MAGTTTIEAVKRKIQVLQQQ ADDAEERAERLQREVEGERR AREQ in isoform 2. 2 PublicationsAdd BLAST	81
Alternative sequenceⁱVSP_006609	190 – 212	KCSEL…LEAQA → RCREMDEQIRLMDQNLKCLS AAE in isoform 2. 2 PublicationsAdd BLAST	23
Alternative sequenceⁱVSP_006610	260 – 285	ELYAQ…DMTSI → KLKCTKEEHLCTQRMLDQTL LDLNEM in isoform 2. 2 PublicationsAdd BLAST	26

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X53753 mRNA. Translation: CAA37782.1. U04541 mRNA. Translation: AAA03725.1. AF317223 mRNA. Translation: AAG38596.1. AK088111 mRNA. Translation: BAC40150.1. AK088902 mRNA. Translation: BAC40643.1. X72633 mRNA. Translation: CAA51209.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS57223.1. [P21107-2]
PIRⁱ	I48852. S11390.
NCBI Reference Sequences More...RefSeqⁱ	NP_001240667.1. NM_001253738.1. [P21107-2] NP_001280677.2. NM_001293748.1. [P21107-1]
UniGeneⁱ	Mm.240839. Mm.421791. Mm.442249. Mm.490309.

Genome annotation databases

Ensemblⁱ	ENSMUST00000029549; ENSMUSP00000029549; ENSMUSG00000027940. [P21107-2]
GeneIDⁱ	59069.
KEGGⁱ	mmu:59069.
UCSC genome browser More...UCSCⁱ	uc008qbf.3. mouse. [P21107-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X53753 mRNA. Translation: CAA37782.1. U04541 mRNA. Translation: AAA03725.1. AF317223 mRNA. Translation: AAG38596.1. AK088111 mRNA. Translation: BAC40150.1. AK088902 mRNA. Translation: BAC40643.1. X72633 mRNA. Translation: CAA51209.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS57223.1. [P21107-2]
PIRⁱ	I48852. S11390.
NCBI Reference Sequences More...RefSeqⁱ	NP_001240667.1. NM_001253738.1. [P21107-2] NP_001280677.2. NM_001293748.1. [P21107-1]
UniGeneⁱ	Mm.240839. Mm.421791. Mm.442249. Mm.490309.

3D structure databases

ProteinModelPortalⁱ	P21107.
SMRⁱ	P21107.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	208522. 5 interactors.
Database of interacting proteins More...DIPⁱ	DIP-32061N.
IntActⁱ	P21107. 5 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1868808.

PTM databases

iPTMnetⁱ	P21107.
PhosphoSitePlusⁱ	P21107.

2D gel databases

COMPLUYEAST-2DPAGEⁱ	P21107.
REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	P21107.
SWISS-2DPAGEⁱ	P21107.

Proteomic databases

EPDⁱ	P21107.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P21107.
PeptideAtlas More...PeptideAtlasⁱ	P21107.
PRIDEⁱ	P21107.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000029549; ENSMUSP00000029549; ENSMUSG00000027940. [P21107-2]
GeneIDⁱ	59069.
KEGGⁱ	mmu:59069.
UCSC genome browser More...UCSCⁱ	uc008qbf.3. mouse. [P21107-1]

Organism-specific databases

CTDⁱ	7170.
MGIⁱ	MGI:1890149. Tpm3.

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074494.
HOGENOMⁱ	HOG000231521.
HOVERGENⁱ	HBG107404.
InParanoidⁱ	P21107.
KEGG Orthology (KO) More...KOⁱ	K09290.

Enzyme and pathway databases

Reactomeⁱ	R-MMU-390522. Striated Muscle Contraction. R-MMU-445355. Smooth Muscle Contraction.

Reactomeⁱ

R-MMU-390522. Striated Muscle Contraction.
R-MMU-445355. Smooth Muscle Contraction.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P21107.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000027940.
CleanExⁱ	MM_TPM3.
ExpressionAtlasⁱ	P21107. baseline and differential.
Genevisibleⁱ	P21107. MM.

Family and domain databases

InterProⁱ	View protein in InterPro IPR000533. Tropomyosin.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00261. Tropomyosin. 1 hit.
PRINTSⁱ	PR00194. TROPOMYOSIN.
PROSITEⁱ	View protein in PROSITE PS00326. TROPOMYOSIN. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TPM3_MOUSE
Accessionⁱ	P21107Primary (citable) accession number: P21107 Secondary accession number(s): Q09021 , Q60606, Q80SW6, Q9EPW3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	June 26, 2013
	Last modified:	May 10, 2017
	This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.Curated

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P21107 (TPM3_MOUSE)

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Tropomyosin alpha-3 chain

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Caution

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequences (2)ⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ