P21097 (3BHS_VACCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
Short name=3-beta-HSD

Including the following 2 domains:

1. 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
   EC=1.1.1.145
   Alternative name(s):
   3-beta-hydroxy-5-ene steroid dehydrogenase
   Progesterone reductase
2. Steroid Delta-isomerase
   EC=5.3.3.1
   Alternative name(s):
   Delta-5-3-ketosteroid isomerase

Gene names

ORF Names:

A44L

Organism

Vaccinia virus (strain Copenhagen) (VACV) [Complete proteome]

Taxonomic identifier

10249 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. During viral infection, steroid production contributes to virulence by inhibiting the host inflammatory response By similarity.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase		PRO_0000087794
Sites
	Active site	147	1	Proton acceptor By similarity
	Binding site	151	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21097 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: F308D8BC9C811469	FASTA	346	39,366
	10 20 30 40 50 60 MAVYAVTGGA GFLGRYIVKL LISADDVQEI RVIDIVEDPQ PITSKVKVIN YIQCDINDFD 70 80 90 100 110 120 KVREALDGVN LIIHTAALVD VFGKYTDNEI MKVNYYGTQT ILAACVDLGI KYLIYTSSME 130 140 150 160 170 180 AIGPNKHGDP FIGHEHTLYD ISPGHVYAKS KRMAEQLVMK ANNSVIMNGA KLYTCCLRPT 190 200 210 220 230 240 GIYGEGDKLT KVFYEQCKQH GNIMYRTVDD NAVHSRVYVG NAAWMHVLAA KYIQYPGSKI 250 260 270 280 290 300 KGNAYFCYDY SPSCSYDMFN LLLMKPLGIE QGSRIPRWML KMYACKNDMK RILFRKPSLL 310 320 330 340 NNYTLKISNT TFEVRTNNAE LDFNYSPIFD VDVAFKRTRK WLEESE

References

[1]	"The complete DNA sequence of vaccinia virus." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:247-266(1990) [PubMed: 2219722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Appendix to 'The complete DNA sequence of vaccinia virus'." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:517-563(1990) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35027 Genomic DNA. Translation: AAA48175.1.
PIR	WMVZ1W. A42522.
3D structure databases
ProteinModelPortal	P21097.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3BHS_VACCC

Accession

Primary (citable) accession number: P21097

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program