P21079 (PAP1_VACCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase catalytic subunit
EC=2.7.7.19

Alternative name(s):
Poly(A) polymerase large subunit
Short name=PAP-L
VP55

Gene names

Name:	PAPL
ORF Names:	E1L

Organism

Vaccinia virus (strain Copenhagen) (VACV) [Complete proteome]

Taxonomic identifier

10249 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.
Sequence similarities	Belongs to the poxviridae poly(A) polymerase catalytic subunit family.

Ontologies

Keywords
Biological process	Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Poly(A) polymerase catalytic subunit		PRO_0000099106
Sites
	Active site	202	1	By similarity
	Active site	204	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21079 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 50AC0C13698BECA7	FASTA	479	55,580
	10 20 30 40 50 60 MNRNPDQNTF PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD IFVSLVKKNK 70 80 90 100 110 120 KRFFSDVDTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTD ILGVLTIKAP 130 140 150 160 170 180 NVISSKISYN VTSMEELARD MLNSMNVAII DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN 190 200 210 220 230 240 KSCICYGSYS LYLINPNIRY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY 250 260 270 280 290 300 MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK 310 320 330 340 350 360 DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD YFSFKKCLVY 370 380 390 400 410 420 LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL 430 440 450 460 470 CAHILLYQML TSGEYKQCLS DLLNSMMNRD KIPIYSHTER DKKPGRHGFI NIEKDIIVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of vaccinia virus." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:247-266(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Appendix to 'The complete DNA sequence of vaccinia virus'." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:517-563(1990) Cited for: COMPLETE GENOME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35027 Genomic DNA. Translation: AAA48038.1.
PIR	E42508.
3D structure databases
ProteinModelPortal	P21079.
SMR	P21079. Positions 12-479.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR024231. Pox_polyA_pol_nucTrfase. IPR004976. Poxvirus_polyA_pol_cat. IPR024397. Poxvirus_polyA_pol_cat_C. IPR024398. Poxvirus_polyA_pol_cat_N. [Graphical view]
Pfam	PF03296. Pox_polyA_pol. 1 hit. PF12629. Pox_polyA_pol_C. 1 hit. PF12630. Pox_polyA_pol_N. 1 hit. [Graphical view]
PIRSF	PIRSF015693. VAC-48L_nuct. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAP1_VACCC

Accession

Primary (citable) accession number: P21079

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program