P21054 (N1_VACCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein N1

Gene names

ORF Names:

N1L

Organism

Vaccinia virus (strain Copenhagen) (VACV) [Complete proteome]

Taxonomic identifier

10249 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Bcl-2-like protein which contributes to virulence by preventing host NF-kappa-B activation in response to pro-inflammatory stimuli such as TNF-alpha or IL1B By similarity.
Sequence similarities	Belongs to the poxviridae N1 protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host NF-kappa-B by virus Modulation of host cell apoptosis by virus Viral immunoevasion
Developmental stage	Early protein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	evasion or tolerance by virus of host immune response Inferred from electronic annotation. Source: UniProtKB-KW modulation by virus of host apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host NF-kappaB transcription factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Protein N1

PRO_0000099632

Secondary structure

1

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117

Helix Strand Turn

Details...

Helix

1 – 14

14

Helix

17 – 19

3

Helix

22 – 28

7

Helix

29 – 33

5

Helix

38 – 49

12

Helix

53 – 59

7

Helix

66 – 83

18

Helix

91 – 102

12

Helix

103 – 105

3

Helix

106 – 113

8

Sequences

	Sequence		Length	Mass (Da)
	P21054 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 9050C1FECEB1B90B	FASTA	117	13,962
	10 20 30 40 50 60 MRTLLIRYIL WRNDNDQTYY NDDFKKLMLL DELVDDGDVC TLIKNMRMTL SDGPLLDRLN 70 80 90 100 110 QPVNNIEDAK RMIAISAKVA RDIGERSEIR WEESFTILFR MIETYFDDLM IDLYGEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of vaccinia virus." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:247-266(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Appendix to 'The complete DNA sequence of vaccinia virus'." Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. Virology 179:517-563(1990) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Vaccinia virus N1L protein resembles a B cell lymphoma-2 (Bcl-2) family protein." Aoyagi M., Zhai D., Jin C., Aleshin A.E., Stec B., Reed J.C., Liddington R.C. Protein Sci. 16:118-124(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[4]	"Functional and structural studies of the vaccinia virus virulence factor N1 reveal a Bcl-2-like anti-apoptotic protein." Cooray S., Bahar M.W., Abrescia N.G., McVey C.E., Bartlett N.W., Chen R.A., Stuart D.I., Grimes J.M., Smith G.L. J. Gen. Virol. 88:1656-1666(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M35027 Genomic DNA. Translation: AAA48001.1.

PIR

E42504.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I39	X-ray	2.20	A/B/C/D/E/F	1-117	[»]
2UXE	X-ray	2.90	A/B/C/D/E/F	1-117	[»]
4BBB	X-ray	3.09	A/B/C/D/E/F	1-117	[»]
4BBC	X-ray	3.10	A/B/C/D/E/F	1-117	[»]
4BBD	X-ray	3.00	A/B/C/D/E/F	1-117	[»]

ProteinModelPortal

P21054.

SMR

P21054. Positions 1-116.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009353. Orthopox_N1.
IPR022819. Poxvirus.
[Graphical view]

Pfam

PF06227. Poxvirus. 1 hit.
[Graphical view]

PIRSF

PIRSF003784. VAC_N1L. 1 hit.

ProDom

PD015763. Orthopox_N1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

BindingDB

P21054.

EvolutionaryTrace

P21054.

Entry information

Entry name

N1_VACCC

Accession

Primary (citable) accession number: P21054

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program