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P21050

- E10_VACCC

UniProt

P21050 - E10_VACCC

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Protein

Probable FAD-linked sulfhydryl oxidase E10

Gene
E10R
Organism
Vaccinia virus (strain Copenhagen) (VACV)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10, A2.5 and G4 By similarity.

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FAD By similarity.

GO - Molecular functioni

  1. thiol oxidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Probable FAD-linked sulfhydryl oxidase E10 (EC:1.8.3.2)
Gene namesi
ORF Names:E10R
OrganismiVaccinia virus (strain Copenhagen) (VACV)
Taxonomic identifieri10249 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008269: Genome

Subcellular locationi

Virion membrane; Single-pass membrane protein Reviewed prediction
Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV) By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88Intravirion Reviewed prediction
Transmembranei9 – 2517Helical; Reviewed predictionAdd
BLAST
Topological domaini26 – 9570Virion surface Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. viral envelope Source: UniProtKB-KW
  3. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9595Probable FAD-linked sulfhydryl oxidase E10PRO_0000099464Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 46Redox-active Reviewed prediction

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9595ERV/ALR sulfhydryl oxidaseAdd
BLAST

Sequence similaritiesi

Belongs to the poxviridae E10 family.

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view]
PfamiPF04805. Pox_E10. 1 hit.
[Graphical view]
PIRSFiPIRSF015696. VAC_E10R. 1 hit.
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21050-1 [UniParc]FASTAAdd to Basket

« Hide

MNPKHWGRAV WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA   50
TIAIEDNNVM SSDDLNYIYY FFIRLFNNLA SDPKYAIDVS KVKPL 95
Length:95
Mass (Da):10,851
Last modified:February 1, 1991 - v1
Checksum:iF4A2FDC41599EA50
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35027 Genomic DNA. Translation: AAA48051.1.
PIRiE42509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35027 Genomic DNA. Translation: AAA48051.1 .
PIRi E42509.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.120.310. 1 hit.
InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view ]
Pfami PF04805. Pox_E10. 1 hit.
[Graphical view ]
PIRSFi PIRSF015696. VAC_E10R. 1 hit.
SUPFAMi SSF69000. SSF69000. 1 hit.
PROSITEi PS51324. ERV_ALR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Appendix to 'The complete DNA sequence of vaccinia virus'."
    Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E.
    Virology 179:517-563(1990)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiE10_VACCC
AccessioniPrimary (citable) accession number: P21050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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