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P21050

- E10_VACCC

UniProt

P21050 - E10_VACCC

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Protein
Probable FAD-linked sulfhydryl oxidase E10
Gene
E10R
Organism
Vaccinia virus (strain Copenhagen) (VACV)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10, A2.5 and G4 By similarity.

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FAD By similarity.

GO - Molecular functioni

  1. thiol oxidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable FAD-linked sulfhydryl oxidase E10 (EC:1.8.3.2)
    Gene namesi
    ORF Names:E10R
    OrganismiVaccinia virus (strain Copenhagen) (VACV)
    Taxonomic identifieri10249 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008269: Genome

    Subcellular locationi

    Virion membrane; Single-pass membrane protein Reviewed prediction
    Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV) By similarity.

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88Intravirion Reviewed prediction
    Transmembranei9 – 2517Helical; Reviewed prediction
    Add
    BLAST
    Topological domaini26 – 9570Virion surface Reviewed prediction
    Add
    BLAST

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. viral envelope Source: UniProtKB-KW
    3. virion membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9595Probable FAD-linked sulfhydryl oxidase E10
    PRO_0000099464Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 46Redox-active Reviewed prediction

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer By similarity.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 9595ERV/ALR sulfhydryl oxidase
    Add
    BLAST

    Sequence similaritiesi

    Belongs to the poxviridae E10 family.

    Keywords - Domaini

    Redox-active center, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.120.310. 1 hit.
    InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
    IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
    [Graphical view]
    PfamiPF04805. Pox_E10. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015696. VAC_E10R. 1 hit.
    SUPFAMiSSF69000. SSF69000. 1 hit.
    PROSITEiPS51324. ERV_ALR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21050-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPKHWGRAV WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA   50
    TIAIEDNNVM SSDDLNYIYY FFIRLFNNLA SDPKYAIDVS KVKPL 95
    Length:95
    Mass (Da):10,851
    Last modified:February 1, 1991 - v1
    Checksum:iF4A2FDC41599EA50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35027 Genomic DNA. Translation: AAA48051.1.
    PIRiE42509.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35027 Genomic DNA. Translation: AAA48051.1 .
    PIRi E42509.

    3D structure databases

    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.120.310. 1 hit.
    InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
    IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
    [Graphical view ]
    Pfami PF04805. Pox_E10. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015696. VAC_E10R. 1 hit.
    SUPFAMi SSF69000. SSF69000. 1 hit.
    PROSITEi PS51324. ERV_ALR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Appendix to 'The complete DNA sequence of vaccinia virus'."
      Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E.
      Virology 179:517-563(1990)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiE10_VACCC
    AccessioniPrimary (citable) accession number: P21050
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: February 19, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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