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P21050

- E10_VACCC

UniProt

P21050 - E10_VACCC

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Protein

Probable FAD-linked sulfhydryl oxidase E10

Gene

E10R

Organism
Vaccinia virus (strain Copenhagen) (VACV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10, A2.5 and G4 (By similarity).PROSITE-ProRule annotation

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FAD.PROSITE-ProRule annotation

GO - Molecular functioni

  1. thiol oxidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Probable FAD-linked sulfhydryl oxidase E10 (EC:1.8.3.2)
Gene namesi
ORF Names:E10R
OrganismiVaccinia virus (strain Copenhagen) (VACV)
Taxonomic identifieri10249 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008269: Genome

Subcellular locationi

Virion membrane Curated; Single-pass membrane protein Curated
Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV).By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9595Probable FAD-linked sulfhydryl oxidase E10PRO_0000099464Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 46Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer.By similarity

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88IntravirionSequence Analysis
Topological domaini26 – 9570Virion surfaceSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2517HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9595ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the poxviridae E10 family.Curated
Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view]
PfamiPF04805. Pox_E10. 1 hit.
[Graphical view]
PIRSFiPIRSF015696. VAC_E10R. 1 hit.
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21050-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPKHWGRAV WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA
60 70 80 90
TIAIEDNNVM SSDDLNYIYY FFIRLFNNLA SDPKYAIDVS KVKPL
Length:95
Mass (Da):10,851
Last modified:February 1, 1991 - v1
Checksum:iF4A2FDC41599EA50
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35027 Genomic DNA. Translation: AAA48051.1.
PIRiE42509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35027 Genomic DNA. Translation: AAA48051.1 .
PIRi E42509.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.120.310. 1 hit.
InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view ]
Pfami PF04805. Pox_E10. 1 hit.
[Graphical view ]
PIRSFi PIRSF015696. VAC_E10R. 1 hit.
SUPFAMi SSF69000. SSF69000. 1 hit.
PROSITEi PS51324. ERV_ALR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Appendix to 'The complete DNA sequence of vaccinia virus'."
    Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E.
    Virology 179:517-563(1990)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiE10_VACCC
AccessioniPrimary (citable) accession number: P21050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3