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P20974

- NAR2B_RAT

UniProt

P20974 - NAR2B_RAT

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Protein

T-cell ecto-ADP-ribosyltransferase 2

Gene

Art2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has both NAD+ glycohydrolase and ADP-ribosyltransferase activity (to a lesser extent).

Catalytic activityi

NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.
NAD+ + H2O = nicotinamide + ADP-ribose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981NAD
Binding sitei146 – 1461NAD
Binding sitei164 – 1641NAD
Binding sitei202 – 2021NAD
Active sitei216 – 2161By similarity

GO - Molecular functioni

  1. hydrolase activity, acting on glycosyl bonds Source: UniProtKB
  2. NAD(P)+-protein-arginine ADP-ribosyltransferase activity Source: RGD

GO - Biological processi

  1. NAD catabolic process Source: UniProtKB
  2. protein ADP-ribosylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell ecto-ADP-ribosyltransferase 2 (EC:2.4.2.31)
Alternative name(s):
ADP-ribosyltransferase C2 and C3 toxin-like 2
Short name:
ARTC2
Alloantigen Rt6.2
Mono(ADP-ribosyl)transferase 2B
T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2
T-cell mono(ADP-ribosyl)transferase 2
T-cell surface protein Rt6.2
Gene namesi
Name:Art2b
Synonyms:Rt6-b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi3521. Art2b.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 246226T-cell ecto-ADP-ribosyltransferase 2PRO_0000019323Add
BLAST
Propeptidei247 – 27529Removed in mature formBy similarityPRO_0000019324Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 243
Disulfide bondi141 ↔ 193
Modified residuei204 – 2041ADP-ribosylarginine; by autocatalysis1 Publication
Lipidationi246 – 2461GPI-anchor amidated serineBy similarity

Keywords - PTMi

ADP-ribosylation, Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiP20974.

Expressioni

Tissue specificityi

Postthymic T-cells.

Gene expression databases

GenevestigatoriP20974.

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi25 – 284Combined sources
Helixi42 – 5918Combined sources
Helixi61 – 7717Combined sources
Helixi78 – 803Combined sources
Helixi89 – 9810Combined sources
Turni99 – 1024Combined sources
Helixi103 – 1119Combined sources
Turni112 – 1154Combined sources
Helixi117 – 1193Combined sources
Helixi123 – 13513Combined sources
Beta strandi142 – 1509Combined sources
Beta strandi152 – 1543Combined sources
Beta strandi156 – 1583Combined sources
Beta strandi166 – 1705Combined sources
Helixi172 – 1754Combined sources
Turni178 – 1803Combined sources
Beta strandi185 – 19410Combined sources
Helixi199 – 2013Combined sources
Helixi205 – 2073Combined sources
Beta strandi209 – 2124Combined sources
Beta strandi216 – 2249Combined sources
Turni225 – 2273Combined sources
Beta strandi228 – 2369Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GXYX-ray1.71A/B21-246[»]
1GXZX-ray2.10A/B21-246[»]
1GY0X-ray2.08A21-246[»]
1OG1X-ray2.00A21-246[»]
1OG3X-ray2.60A21-246[»]
1OG4X-ray2.60A21-246[»]
ProteinModelPortaliP20974.
SMRiP20974. Positions 24-246.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20974.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00530000062975.
HOVERGENiHBG004464.
KOiK00775.
OMAiYNEIFLD.
OrthoDBiEOG7D85WV.
PhylomeDBiP20974.
TreeFamiTF335356.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PANTHERiPTHR10339. PTHR10339. 1 hit.
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20974-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSNICKFFL TWWLIQQVTG LTGPLMLDTA PNAFDDQYEG CVNKMEEKAP
60 70 80 90 100
LLLQEDFNMN AKLKVAWEEA KKRWNNIKPS RSYPKGFNDF HGTALVAYTG
110 120 130 140 150
SIAVDFNRAV REFKENPGQF HYKAFHYYLT RALQLLSNGD CHSVYRGTKT
160 170 180 190 200
RFHYTGAGSV RFGQFTSSSL SKKVAQSQEF FSDHGTLFII KTCLGVYIKE
210 220 230 240 250
FSFRPDQEEV LIPGYEVYQK VRTQGYNEIF LDSPKRKKSN YNCLYSSAGA
260 270
RESCVSLFLV VLPSLLVQLL CLAEP
Length:275
Mass (Da):31,438
Last modified:July 15, 1998 - v2
Checksum:iB3361D4E6FF77FC4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291T → K in CAC20897. (PubMed:11220625)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85193 mRNA. Translation: AAA42085.1.
AJ297708 Genomic DNA. Translation: CAC20897.1.
BC099070 mRNA. Translation: AAH99070.1.
X99123 mRNA. Translation: CAA67566.1.
X99122 mRNA. Translation: CAA67565.1.
PIRiA34866.
RefSeqiNP_942030.1. NM_198735.2.
XP_008757920.1. XM_008759698.1.
UniGeneiRn.107075.
Rn.214239.

Genome annotation databases

EnsembliENSRNOT00000026644; ENSRNOP00000026644; ENSRNOG00000019687.
ENSRNOT00000076714; ENSRNOP00000068409; ENSRNOG00000019687.
GeneIDi293152.
KEGGirno:293152.
UCSCiRGD:3521. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85193 mRNA. Translation: AAA42085.1 .
AJ297708 Genomic DNA. Translation: CAC20897.1 .
BC099070 mRNA. Translation: AAH99070.1 .
X99123 mRNA. Translation: CAA67566.1 .
X99122 mRNA. Translation: CAA67565.1 .
PIRi A34866.
RefSeqi NP_942030.1. NM_198735.2.
XP_008757920.1. XM_008759698.1.
UniGenei Rn.107075.
Rn.214239.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GXY X-ray 1.71 A/B 21-246 [» ]
1GXZ X-ray 2.10 A/B 21-246 [» ]
1GY0 X-ray 2.08 A 21-246 [» ]
1OG1 X-ray 2.00 A 21-246 [» ]
1OG3 X-ray 2.60 A 21-246 [» ]
1OG4 X-ray 2.60 A 21-246 [» ]
ProteinModelPortali P20974.
SMRi P20974. Positions 24-246.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P20974.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000026644 ; ENSRNOP00000026644 ; ENSRNOG00000019687 .
ENSRNOT00000076714 ; ENSRNOP00000068409 ; ENSRNOG00000019687 .
GeneIDi 293152.
KEGGi rno:293152.
UCSCi RGD:3521. rat.

Organism-specific databases

CTDi 11872.
RGDi 3521. Art2b.

Phylogenomic databases

GeneTreei ENSGT00530000062975.
HOVERGENi HBG004464.
KOi K00775.
OMAi YNEIFLD.
OrthoDBi EOG7D85WV.
PhylomeDBi P20974.
TreeFami TF335356.

Miscellaneous databases

EvolutionaryTracei P20974.
NextBioi 635465.

Gene expression databases

Genevestigatori P20974.

Family and domain databases

InterProi IPR000768. ART.
[Graphical view ]
PANTHERi PTHR10339. PTHR10339. 1 hit.
Pfami PF01129. ART. 1 hit.
[Graphical view ]
PRINTSi PR00970. RIBTRNSFRASE.
PROSITEi PS01291. ART. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of rat RT6.2, a nonglycosylated phosphatidylinositol-linked surface marker of postthymic T cells."
    Koch F., Haag F., Kashan A., Thiele H.-G.
    Proc. Natl. Acad. Sci. U.S.A. 87:964-967(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "DNA methylation contributes to tissue- and allele-specific expression of the T-cell differentiation marker RT6."
    Rothenburg S., Koch-Nolte F., Thiele H.-G., Haag F.
    Immunogenetics 52:231-241(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BH.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  4. "Structure of the gene encoding the rat T cell ecto-ADP-ribosyltransferase RT6."
    Haag F., Kuhlenbaumer G., Koch-Nolte F., Wingender E., Thiele H.-G.
    J. Immunol. 157:2022-2030(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-201.
    Strain: DA.
    Tissue: Spleen.
  5. "A single-step purification procedure and partial amino acid sequence analysis of picomole amounts of the rat T cell alloantigen RT6.2."
    Kashan A., Buck F., Haag F., Koch F., Thiele H.-G.
    Immunol. Lett. 23:133-138(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
  6. "Expression of NAD glycohydrolase activity by rat mammary adenocarcinoma cells transformed with rat T cell alloantigen RT6.2."
    Takada T., Iida K., Moss J.
    J. Biol. Chem. 269:9420-9423(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Structure of the ecto-ADP-ribosyl transferase ART2.2 from rat."
    Mueller-Dieckmann C., Ritter H., Haag F., Koch-Nolte F., Schulz G.E.
    J. Mol. Biol. 322:687-696(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.71 ANGSTROMS) OF 21-246, DISULFIDE BONDS.
  8. "Substrate binding and catalysis of ecto-ADP-ribosyltransferase 2.2 from rat."
    Ritter H., Koch-Nolte F., Marquez V.E., Schulz G.E.
    Biochemistry 42:10155-10162(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 21-246 OF WILD-TYPE AND MUTANTS ILE-189 AND ALA-189 IN COMPLEX WITH NAD(+), ADP-RIBOSYLATION AT ARG-204, DISULFIDE BONDS.

Entry informationi

Entry nameiNAR2B_RAT
AccessioniPrimary (citable) accession number: P20974
Secondary accession number(s): P97912
, Q4FZV8, Q95576, Q9EPH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 15, 1998
Last modified: November 26, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3