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P20971 (PGK_METFV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:Mfer_0156
OrganismMethanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S) [Complete proteome] [HAMAP]
Taxonomic identifier523846 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Homodimer.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000146057

Regions

Nucleotide binding358 – 3614ATP By similarity
Region22 – 243Substrate binding By similarity
Region62 – 654Substrate binding By similarity

Sites

Binding site391Substrate By similarity
Binding site1191Substrate By similarity
Binding site1591Substrate By similarity
Binding site3321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P20971 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 6AA54EB65C292EFA

FASTA41045,871
        10         20         30         40         50         60 
MFKFYTMDDF DYSGSRVLVR VDINSPVDPH TGRILDDTRM RLHSKTLKEL VDENAKVAIL 

        70         80         90        100        110        120 
AHQSRPGKRD FTTMEEHSKV LSNILDMPVT YVEDIFGCAA RESIRNMENG DIILLENVRF 

       130        140        150        160        170        180 
YSEEVLKRDP KVQAETHLVR KLSSVVDYYI NDAFAAAHRS QPSLVGFPLK LPSAAGRLME 

       190        200        210        220        230        240 
REVKTLYKII KNVEKPCVYI LGGVKIDDSI MIMKNILKNG SADYILTSGL VANVFLEASG 

       250        260        270        280        290        300 
IDIKEKNRKI LYRKNYKKFI KMAKKLKDKY GEKILTPVDV AINKNGKRID VPIDDIPNFP 

       310        320        330        340        350        360 
IYDIGMETIK IYAEKIREAK TIFANGPAGV FEEQQFSIGT EDLLNAIASS NAFSVIAGGH 

       370        380        390        400        410 
LAAAAEKMGI SNKINHISSG GGACIAFLSG EELPAIKVLE EARKRSDKYI 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing the gene encoding 3-phosphoglycerate kinase from mesophilic Methanobacterium bryantii and thermophilic Methanothermus fervidus."
Fabry S., Heppner P., Dietmaier W., Hensel R.
Gene 91:19-25(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
[2]"Complete genome sequence of Methanothermus fervidus type strain (V24S)."
Anderson I., Djao O.D., Misra M., Chertkov O., Nolan M., Lucas S., Lapidus A., Del Rio T.G., Tice H., Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S., Liolios K., Ivanova N., Mavromatis K., Mikhailova N. expand/collapse author list , Pati A., Brambilla E., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Sikorski J., Spring S., Rohde M., Eichinger K., Huber H., Wirth R., Goker M., Detter J.C., Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P., Kyrpides N.C.
Stand. Genomic Sci. 3:315-324(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55529 Genomic DNA. Translation: AAA72936.1.
CP002278 Genomic DNA. Translation: ADP76959.1.
PIRPN0008.
RefSeqYP_004003721.1. NC_014658.1.

3D structure databases

ProteinModelPortalP20971.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADP76959; ADP76959; Mfer_0156.
GeneID9961867.
KEGGmfv:Mfer_0156.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000227108.
KOK00927.
OMADMIFDIG.

Enzyme and pathway databases

BioCycMFER523846:GC24-165-MONOMER.
SABIO-RKP20971.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK_METFV
AccessionPrimary (citable) accession number: P20971
Secondary accession number(s): E3GXC7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways