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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathway: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap), Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391SubstrateBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei159 – 1591SubstrateBy similarity
Binding sitei332 – 3321ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi358 – 3614ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMFER523846:GC24-165-MONOMER.
BRENDAi2.7.2.3. 3286.
SABIO-RKP20971.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:Mfer_0156
OrganismiMethanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
Taxonomic identifieri523846 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus
ProteomesiUP000002315 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Phosphoglycerate kinasePRO_0000146057Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi523846.Mfer_0156.

Structurei

3D structure databases

ProteinModelPortaliP20971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 243Substrate bindingBy similarity
Regioni62 – 654Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000227108.
KOiK00927.
OMAiDAFGTCH.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKFYTMDDF DYSGSRVLVR VDINSPVDPH TGRILDDTRM RLHSKTLKEL
60 70 80 90 100
VDENAKVAIL AHQSRPGKRD FTTMEEHSKV LSNILDMPVT YVEDIFGCAA
110 120 130 140 150
RESIRNMENG DIILLENVRF YSEEVLKRDP KVQAETHLVR KLSSVVDYYI
160 170 180 190 200
NDAFAAAHRS QPSLVGFPLK LPSAAGRLME REVKTLYKII KNVEKPCVYI
210 220 230 240 250
LGGVKIDDSI MIMKNILKNG SADYILTSGL VANVFLEASG IDIKEKNRKI
260 270 280 290 300
LYRKNYKKFI KMAKKLKDKY GEKILTPVDV AINKNGKRID VPIDDIPNFP
310 320 330 340 350
IYDIGMETIK IYAEKIREAK TIFANGPAGV FEEQQFSIGT EDLLNAIASS
360 370 380 390 400
NAFSVIAGGH LAAAAEKMGI SNKINHISSG GGACIAFLSG EELPAIKVLE
410
EARKRSDKYI
Length:410
Mass (Da):45,871
Last modified:February 1, 1991 - v1
Checksum:i6AA54EB65C292EFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55529 Genomic DNA. Translation: AAA72936.1.
CP002278 Genomic DNA. Translation: ADP76959.1.
PIRiPN0008.
RefSeqiWP_013413237.1. NC_014658.1.
YP_004003721.1. NC_014658.1.

Genome annotation databases

EnsemblBacteriaiADP76959; ADP76959; Mfer_0156.
GeneIDi9961867.
KEGGimfv:Mfer_0156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55529 Genomic DNA. Translation: AAA72936.1.
CP002278 Genomic DNA. Translation: ADP76959.1.
PIRiPN0008.
RefSeqiWP_013413237.1. NC_014658.1.
YP_004003721.1. NC_014658.1.

3D structure databases

ProteinModelPortaliP20971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi523846.Mfer_0156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADP76959; ADP76959; Mfer_0156.
GeneIDi9961867.
KEGGimfv:Mfer_0156.

Phylogenomic databases

HOGENOMiHOG000227108.
KOiK00927.
OMAiDAFGTCH.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciMFER523846:GC24-165-MONOMER.
BRENDAi2.7.2.3. 3286.
SABIO-RKP20971.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing the gene encoding 3-phosphoglycerate kinase from mesophilic Methanobacterium bryantii and thermophilic Methanothermus fervidus."
    Fabry S., Heppner P., Dietmaier W., Hensel R.
    Gene 91:19-25(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.

Entry informationi

Entry nameiPGK_METFV
AccessioniPrimary (citable) accession number: P20971
Secondary accession number(s): E3GXC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 24, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.