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P20967

- ODO1_YEAST

UniProt

P20967 - ODO1_YEAST

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Protein

2-oxoglutarate dehydrogenase, mitochondrial

Gene

KGD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).3 Publications

Catalytic activityi

2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.

Cofactori

Enzyme regulationi

Catabolite repressed.

GO - Molecular functioni

  1. oxoglutarate dehydrogenase (succinyl-transferring) activity Source: SGD
  2. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. 2-oxoglutarate metabolic process Source: SGD
  2. tricarboxylic acid cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciYEAST:YIL125W-MONOMER.
ReactomeiREACT_245486. Citric acid cycle (TCA cycle).
REACT_258556. Lysine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoglutarate dehydrogenase, mitochondrial (EC:1.2.4.2)
Alternative name(s):
2-oxoglutarate dehydrogenase complex component E1
Short name:
OGDC-E1
Alpha-ketoglutarate dehydrogenase
Gene namesi
Name:KGD1
Synonyms:OGD1
Ordered Locus Names:YIL125W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL125w.
SGDiS000001387. KGD1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial nucleoid Source: SGD
  2. mitochondrial oxoglutarate dehydrogenase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Mitochondrion nucleoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionSequence AnalysisAdd
BLAST
Chaini31 – 10149842-oxoglutarate dehydrogenase, mitochondrialPRO_0000020436Add
BLAST

Proteomic databases

MaxQBiP20967.
PaxDbiP20967.
PeptideAtlasiP20967.
PRIDEiP20967.

Expressioni

Gene expression databases

GenevestigatoriP20967.

Interactioni

Protein-protein interaction databases

BioGridi34866. 48 interactions.
DIPiDIP-365N.
IntActiP20967. 35 interactions.
MINTiMINT-623074.
STRINGi4932.YIL125W.

Structurei

3D structure databases

ProteinModelPortaliP20967.
SMRiP20967. Positions 124-1011.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0567.
GeneTreeiENSGT00530000063092.
HOGENOMiHOG000259586.
InParanoidiP20967.
KOiK00164.
OMAiQHAPNKE.
OrthoDBiEOG72G1GM.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
InterProiIPR011603. 2oxoglutarate_DH_E1.
IPR001017. DH_E1.
IPR029061. THDP-binding.
IPR005475. Transketolase-like_Pyr-bd.
[Graphical view]
PANTHERiPTHR23152. PTHR23152. 1 hit.
PfamiPF00676. E1_dh. 1 hit.
PF02779. Transket_pyr. 1 hit.
[Graphical view]
PIRSFiPIRSF000157. Oxoglu_dh_E1. 1 hit.
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00239. 2oxo_dh_E1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20967-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRFVSSQTC RYSSRGLLKT SLLKNASTVK IVGRGLATTG TDNFLSTSNA
60 70 80 90 100
TYIDEMYQAW QKDPSSVHVS WDAYFKNMSN PKIPATKAFQ APPSISNFPQ
110 120 130 140 150
GTEAAPLGTA MTGSVDENVS IHLKVQLLCR AYQVRGHLKA HIDPLGISFG
160 170 180 190 200
SNKNNPVPPE LTLDYYGFSK HDLDKEINLG PGILPRFARD GKSKMSLKEI
210 220 230 240 250
VDHLEKLYCS SYGVQYTHIP SKQKCDWLRE RIEIPEPYQY TVDQKRQILD
260 270 280 290 300
RLTWATSFES FLSTKFPNDK RFGLEGLESV VPGIKTLVDR SVELGVEDIV
310 320 330 340 350
LGMAHRGRLN VLSNVVRKPN ESIFSEFKGS SARDDIEGSG DVKYHLGMNY
360 370 380 390 400
QRPTTSGKYV NLSLVANPSH LESQDPVVLG RTRALLHAKN DLKEKTKALG
410 420 430 440 450
VLLHGDAAFA GQGVVYETMG FLTLPEYSTG GTIHVITNNQ IGFTTDPRFA
460 470 480 490 500
RSTPYPSDLA KAIDAPIFHV NANDVEAVTF IFNLAAEWRH KFHTDAIIDV
510 520 530 540 550
VGWRKHGHNE TDQPSFTQPL MYKKIAKQKS VIDVYTEKLI SEGTFSKKDI
560 570 580 590 600
DEHKKWVWNL FEDAFEKAKD YVPSQREWLT AAWEGFKSPK ELATEILPHE
610 620 630 640 650
PTNVPESTLK ELGKVLSSWP EGFEVHKNLK RILKNRGKSI ETGEGIDWAT
660 670 680 690 700
GEALAFGTLV LDGQNVRVSG EDVERGTFSQ RHAVLHDQQS EAIYTPLSTL
710 720 730 740 750
NNEKADFTIA NSSLSEYGVM GFEYGYSLTS PDYLVMWEAQ FGDFANTAQV
760 770 780 790 800
IIDQFIAGGE QKWKQRSGLV LSLPHGYDGQ GPEHSSGRLE RFLQLANEDP
810 820 830 840 850
RYFPSEEKLQ RQHQDCNFQV VYPTTPANLF HILRRQQHRQ FRKPLALFFS
860 870 880 890 900
KQLLRHPLAR SSLSEFTEGG FQWIIEDIEH GKSIGTKEET KRLVLLSGQV
910 920 930 940 950
YTALHKRRES LGDKTTAFLK IEQLHPFPFA QLRDSLNSYP NLEEIVWCQE
960 970 980 990 1000
EPLNMGSWAY TEPRLHTTLK ETDKYKDFKV RYCGRNPSGA VAAGSKSLHL
1010
AEEDAFLKDV FQQS
Length:1,014
Mass (Da):114,416
Last modified:February 1, 1995 - v2
Checksum:iEF987C4DECE3F09F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti325 – 3273SEF → LNL in AAA34721. (PubMed:2503710)Curated
Sequence conflicti513 – 5131Q → R in AAA34721. (PubMed:2503710)Curated
Sequence conflicti568 – 5681A → T in AAA34721. (PubMed:2503710)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26390 Genomic DNA. Translation: AAA34721.1.
Z46833 Genomic DNA. Translation: CAA86867.1.
BK006942 Genomic DNA. Translation: DAA08428.1.
PIRiS49884. DEBY.
RefSeqiNP_012141.1. NM_001179473.1.

Genome annotation databases

EnsemblFungiiYIL125W; YIL125W; YIL125W.
GeneIDi854681.
KEGGisce:YIL125W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26390 Genomic DNA. Translation: AAA34721.1 .
Z46833 Genomic DNA. Translation: CAA86867.1 .
BK006942 Genomic DNA. Translation: DAA08428.1 .
PIRi S49884. DEBY.
RefSeqi NP_012141.1. NM_001179473.1.

3D structure databases

ProteinModelPortali P20967.
SMRi P20967. Positions 124-1011.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34866. 48 interactions.
DIPi DIP-365N.
IntActi P20967. 35 interactions.
MINTi MINT-623074.
STRINGi 4932.YIL125W.

Proteomic databases

MaxQBi P20967.
PaxDbi P20967.
PeptideAtlasi P20967.
PRIDEi P20967.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL125W ; YIL125W ; YIL125W .
GeneIDi 854681.
KEGGi sce:YIL125W.

Organism-specific databases

CYGDi YIL125w.
SGDi S000001387. KGD1.

Phylogenomic databases

eggNOGi COG0567.
GeneTreei ENSGT00530000063092.
HOGENOMi HOG000259586.
InParanoidi P20967.
KOi K00164.
OMAi QHAPNKE.
OrthoDBi EOG72G1GM.

Enzyme and pathway databases

BioCyci YEAST:YIL125W-MONOMER.
Reactomei REACT_245486. Citric acid cycle (TCA cycle).
REACT_258556. Lysine catabolism.

Miscellaneous databases

NextBioi 977289.
PROi P20967.

Gene expression databases

Genevestigatori P20967.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
InterProi IPR011603. 2oxoglutarate_DH_E1.
IPR001017. DH_E1.
IPR029061. THDP-binding.
IPR005475. Transketolase-like_Pyr-bd.
[Graphical view ]
PANTHERi PTHR23152. PTHR23152. 1 hit.
Pfami PF00676. E1_dh. 1 hit.
PF02779. Transket_pyr. 1 hit.
[Graphical view ]
PIRSFi PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMARTi SM00861. Transket_pyr. 1 hit.
[Graphical view ]
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and regulation of KGD1, the structural gene for yeast alpha-ketoglutarate dehydrogenase."
    Repetto B., Tzagoloff A.
    Mol. Cell. Biol. 9:2695-2705(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 208353 / W303-1A.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The ogd1 and kgd1 mutants lacking 2-oxoglutarate dehydrogenase activity in yeast are allelic and can be differentiated by the cloned amber suppressor."
    Mockovciakova D., Janitorova V., Zigova M., Kaclikova E., Zagulski M., Subik J.
    Curr. Genet. 24:377-381(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Identification of the YMN-1 antigen protein and biochemical analyses of protein components in the mitochondrial nucleoid fraction of the yeast Saccharomyces cerevisiae."
    Sato H., Tachifuji A., Tamura M., Miyakawa I.
    Protoplasma 219:51-58(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiODO1_YEAST
AccessioniPrimary (citable) accession number: P20967
Secondary accession number(s): D6VVG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 14300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3