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P20967

- ODO1_YEAST

UniProt

P20967 - ODO1_YEAST

Protein

2-oxoglutarate dehydrogenase, mitochondrial

Gene

KGD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).3 Publications

    Catalytic activityi

    2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.

    Cofactori

    Thiamine pyrophosphate.

    Enzyme regulationi

    Catabolite repressed.

    GO - Molecular functioni

    1. oxoglutarate dehydrogenase (succinyl-transferring) activity Source: SGD
    2. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. 2-oxoglutarate metabolic process Source: SGD
    2. tricarboxylic acid cycle Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciYEAST:YIL125W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-oxoglutarate dehydrogenase, mitochondrial (EC:1.2.4.2)
    Alternative name(s):
    2-oxoglutarate dehydrogenase complex component E1
    Short name:
    OGDC-E1
    Alpha-ketoglutarate dehydrogenase
    Gene namesi
    Name:KGD1
    Synonyms:OGD1
    Ordered Locus Names:YIL125W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL125w.
    SGDiS000001387. KGD1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial nucleoid Source: SGD
    2. mitochondrial oxoglutarate dehydrogenase complex Source: SGD

    Keywords - Cellular componenti

    Mitochondrion, Mitochondrion nucleoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030MitochondrionSequence AnalysisAdd
    BLAST
    Chaini31 – 10149842-oxoglutarate dehydrogenase, mitochondrialPRO_0000020436Add
    BLAST

    Proteomic databases

    MaxQBiP20967.
    PaxDbiP20967.
    PeptideAtlasiP20967.
    PRIDEiP20967.

    Expressioni

    Gene expression databases

    GenevestigatoriP20967.

    Interactioni

    Protein-protein interaction databases

    BioGridi34866. 48 interactions.
    DIPiDIP-365N.
    IntActiP20967. 35 interactions.
    MINTiMINT-623074.
    STRINGi4932.YIL125W.

    Structurei

    3D structure databases

    ProteinModelPortaliP20967.
    SMRiP20967. Positions 124-1011.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0567.
    GeneTreeiENSGT00530000063092.
    HOGENOMiHOG000259586.
    KOiK00164.
    OMAiQHAPNKE.
    OrthoDBiEOG72G1GM.

    Family and domain databases

    Gene3Di3.40.50.970. 2 hits.
    InterProiIPR011603. 2oxoglutarate_DH_E1.
    IPR001017. DH_E1.
    IPR029061. THDP-binding.
    IPR005475. Transketolase-like_Pyr-bd.
    [Graphical view]
    PANTHERiPTHR23152. PTHR23152. 1 hit.
    PfamiPF00676. E1_dh. 1 hit.
    PF02779. Transket_pyr. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000157. Oxoglu_dh_E1. 1 hit.
    SMARTiSM00861. Transket_pyr. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 2 hits.
    TIGRFAMsiTIGR00239. 2oxo_dh_E1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20967-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRFVSSQTC RYSSRGLLKT SLLKNASTVK IVGRGLATTG TDNFLSTSNA     50
    TYIDEMYQAW QKDPSSVHVS WDAYFKNMSN PKIPATKAFQ APPSISNFPQ 100
    GTEAAPLGTA MTGSVDENVS IHLKVQLLCR AYQVRGHLKA HIDPLGISFG 150
    SNKNNPVPPE LTLDYYGFSK HDLDKEINLG PGILPRFARD GKSKMSLKEI 200
    VDHLEKLYCS SYGVQYTHIP SKQKCDWLRE RIEIPEPYQY TVDQKRQILD 250
    RLTWATSFES FLSTKFPNDK RFGLEGLESV VPGIKTLVDR SVELGVEDIV 300
    LGMAHRGRLN VLSNVVRKPN ESIFSEFKGS SARDDIEGSG DVKYHLGMNY 350
    QRPTTSGKYV NLSLVANPSH LESQDPVVLG RTRALLHAKN DLKEKTKALG 400
    VLLHGDAAFA GQGVVYETMG FLTLPEYSTG GTIHVITNNQ IGFTTDPRFA 450
    RSTPYPSDLA KAIDAPIFHV NANDVEAVTF IFNLAAEWRH KFHTDAIIDV 500
    VGWRKHGHNE TDQPSFTQPL MYKKIAKQKS VIDVYTEKLI SEGTFSKKDI 550
    DEHKKWVWNL FEDAFEKAKD YVPSQREWLT AAWEGFKSPK ELATEILPHE 600
    PTNVPESTLK ELGKVLSSWP EGFEVHKNLK RILKNRGKSI ETGEGIDWAT 650
    GEALAFGTLV LDGQNVRVSG EDVERGTFSQ RHAVLHDQQS EAIYTPLSTL 700
    NNEKADFTIA NSSLSEYGVM GFEYGYSLTS PDYLVMWEAQ FGDFANTAQV 750
    IIDQFIAGGE QKWKQRSGLV LSLPHGYDGQ GPEHSSGRLE RFLQLANEDP 800
    RYFPSEEKLQ RQHQDCNFQV VYPTTPANLF HILRRQQHRQ FRKPLALFFS 850
    KQLLRHPLAR SSLSEFTEGG FQWIIEDIEH GKSIGTKEET KRLVLLSGQV 900
    YTALHKRRES LGDKTTAFLK IEQLHPFPFA QLRDSLNSYP NLEEIVWCQE 950
    EPLNMGSWAY TEPRLHTTLK ETDKYKDFKV RYCGRNPSGA VAAGSKSLHL 1000
    AEEDAFLKDV FQQS 1014
    Length:1,014
    Mass (Da):114,416
    Last modified:February 1, 1995 - v2
    Checksum:iEF987C4DECE3F09F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti325 – 3273SEF → LNL in AAA34721. (PubMed:2503710)Curated
    Sequence conflicti513 – 5131Q → R in AAA34721. (PubMed:2503710)Curated
    Sequence conflicti568 – 5681A → T in AAA34721. (PubMed:2503710)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26390 Genomic DNA. Translation: AAA34721.1.
    Z46833 Genomic DNA. Translation: CAA86867.1.
    BK006942 Genomic DNA. Translation: DAA08428.1.
    PIRiS49884. DEBY.
    RefSeqiNP_012141.1. NM_001179473.1.

    Genome annotation databases

    EnsemblFungiiYIL125W; YIL125W; YIL125W.
    GeneIDi854681.
    KEGGisce:YIL125W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26390 Genomic DNA. Translation: AAA34721.1 .
    Z46833 Genomic DNA. Translation: CAA86867.1 .
    BK006942 Genomic DNA. Translation: DAA08428.1 .
    PIRi S49884. DEBY.
    RefSeqi NP_012141.1. NM_001179473.1.

    3D structure databases

    ProteinModelPortali P20967.
    SMRi P20967. Positions 124-1011.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34866. 48 interactions.
    DIPi DIP-365N.
    IntActi P20967. 35 interactions.
    MINTi MINT-623074.
    STRINGi 4932.YIL125W.

    Proteomic databases

    MaxQBi P20967.
    PaxDbi P20967.
    PeptideAtlasi P20967.
    PRIDEi P20967.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL125W ; YIL125W ; YIL125W .
    GeneIDi 854681.
    KEGGi sce:YIL125W.

    Organism-specific databases

    CYGDi YIL125w.
    SGDi S000001387. KGD1.

    Phylogenomic databases

    eggNOGi COG0567.
    GeneTreei ENSGT00530000063092.
    HOGENOMi HOG000259586.
    KOi K00164.
    OMAi QHAPNKE.
    OrthoDBi EOG72G1GM.

    Enzyme and pathway databases

    BioCyci YEAST:YIL125W-MONOMER.

    Miscellaneous databases

    NextBioi 977289.
    PROi P20967.

    Gene expression databases

    Genevestigatori P20967.

    Family and domain databases

    Gene3Di 3.40.50.970. 2 hits.
    InterProi IPR011603. 2oxoglutarate_DH_E1.
    IPR001017. DH_E1.
    IPR029061. THDP-binding.
    IPR005475. Transketolase-like_Pyr-bd.
    [Graphical view ]
    PANTHERi PTHR23152. PTHR23152. 1 hit.
    Pfami PF00676. E1_dh. 1 hit.
    PF02779. Transket_pyr. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000157. Oxoglu_dh_E1. 1 hit.
    SMARTi SM00861. Transket_pyr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 2 hits.
    TIGRFAMsi TIGR00239. 2oxo_dh_E1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and regulation of KGD1, the structural gene for yeast alpha-ketoglutarate dehydrogenase."
      Repetto B., Tzagoloff A.
      Mol. Cell. Biol. 9:2695-2705(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 208353 / W303-1A.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The ogd1 and kgd1 mutants lacking 2-oxoglutarate dehydrogenase activity in yeast are allelic and can be differentiated by the cloned amber suppressor."
      Mockovciakova D., Janitorova V., Zigova M., Kaclikova E., Zagulski M., Subik J.
      Curr. Genet. 24:377-381(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Identification of the YMN-1 antigen protein and biochemical analyses of protein components in the mitochondrial nucleoid fraction of the yeast Saccharomyces cerevisiae."
      Sato H., Tachifuji A., Tamura M., Miyakawa I.
      Protoplasma 219:51-58(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiODO1_YEAST
    AccessioniPrimary (citable) accession number: P20967
    Secondary accession number(s): D6VVG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 14300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3