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Protein

PTS system fructose-specific EIIB'BC component

Gene

fruA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FruAB PTS system is involved in fructose transport.1 Publication2 Publications

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).1 Publication1 Publication

Kineticsi

  1. KM=5.4 µM for fructose1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei112Phosphocysteine intermediate; for EIIB activity1 Publication1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Phosphotransferase system, Sugar transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:FRUA-MONOMER.
    ECOL316407:JW2154-MONOMER.
    MetaCyc:FRUA-MONOMER.

    Protein family/group databases

    TCDBi4.A.2.1.1. the pts fructose-mannitol (fru) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PTS system fructose-specific EIIB'BC component1 Publication
    Alternative name(s):
    EIIB'BC-Fru1 Publication
    Including the following 2 domains:
    PTS system fructose-specific EIIB component1 Publication (EC:2.7.1.2021 Publication1 Publication)
    Alternative name(s):
    EIII-Fru1 Publication
    Fructose-specific phosphotransferase enzyme IIB component1 Publication
    PTS system fructose-specific EIIC component1 Publication
    Alternative name(s):
    Fructose permease IIC component1 Publication
    Gene namesi
    Name:fruA1 Publication
    Synonyms:ptsF
    Ordered Locus Names:b2167, JW2154
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10336. fruA.

    Subcellular locationi

    • Cell inner membrane PROSITE-ProRule annotation1 Publication1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation2 Publications1 Publication

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei236 – 256HelicalPROSITE-ProRule annotationAdd BLAST21
    Transmembranei274 – 294HelicalPROSITE-ProRule annotationAdd BLAST21
    Transmembranei304 – 324HelicalPROSITE-ProRule annotationAdd BLAST21
    Transmembranei349 – 369HelicalPROSITE-ProRule annotationAdd BLAST21
    Transmembranei382 – 402HelicalPROSITE-ProRule annotationAdd BLAST21
    Transmembranei430 – 450HelicalPROSITE-ProRule annotationAdd BLAST21
    Transmembranei463 – 483HelicalPROSITE-ProRule annotationAdd BLAST21
    Transmembranei489 – 509HelicalPROSITE-ProRule annotationAdd BLAST21
    Transmembranei518 – 538HelicalPROSITE-ProRule annotationAdd BLAST21

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001865081 – 563PTS system fructose-specific EIIB'BC componentAdd BLAST563

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei112Phosphocysteine; by EIIAPROSITE-ProRule annotation1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    EPDiP20966.
    PaxDbiP20966.
    PRIDEiP20966.

    Expressioni

    Inductioni

    By fructose.By similarity

    Interactioni

    Protein-protein interaction databases

    BioGridi4260463. 14 interactors.
    IntActiP20966. 2 interactors.
    STRINGi511145.b2167.

    Structurei

    3D structure databases

    ProteinModelPortaliP20966.
    SMRiP20966.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 85PTS EIIB type-2 11 PublicationAdd BLAST85
    Domaini104 – 201PTS EIIB type-2 2PROSITE-ProRule annotationAdd BLAST98
    Domaini226 – 561PTS EIIC type-2PROSITE-ProRule annotationAdd BLAST336

    Domaini

    The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation
    In the N-terminal, the PTS system fructose-specific possesses a duplicated EIIB domain (EIIB' domain) which lacks the active site and functions to facilitate phosphoryl transfer between the EIIA domain of diphosphoryl transfer protein (DTP) and the EIIB domain. Construction of a protein lacking the EIIB' domain shows that it is functional for fructose transport in vivo as well as fructose phosphorylation in vitro. The presence of the EIIB' domain, however, is required for normal high affinity recognition of DTP by the PTS system fructose-specific as well as for normal rates of phosphoryl transfer between the EIIA and EIIB domains of DTP and PTS system fructose-specific, respectively.1 Publication
    The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation

    Sequence similaritiesi

    Contains 2 PTS EIIB type-2 domains.PROSITE-ProRule annotation
    Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105DM4. Bacteria.
    COG1299. LUCA.
    COG1445. LUCA.
    COG3925. LUCA.
    HOGENOMiHOG000227677.
    InParanoidiP20966.
    KOiK02769.
    K02770.
    OMAiHAVILAC.
    PhylomeDBiP20966.

    Family and domain databases

    CDDicd05569. PTS_IIB_fructose. 1 hit.
    InterProiIPR013011. PTS_EIIB_2.
    IPR003501. PTS_EIIB_2/3.
    IPR003352. PTS_EIIC.
    IPR013014. PTS_EIIC_2.
    IPR003353. PTS_IIB_fruc.
    IPR006327. PTS_IIC_fruc.
    [Graphical view]
    PfamiPF02378. PTS_EIIC. 1 hit.
    PF02302. PTS_IIB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52794. SSF52794. 2 hits.
    TIGRFAMsiTIGR00829. FRU. 1 hit.
    TIGR01427. PTS_IIC_fructo. 1 hit.
    PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
    PS51104. PTS_EIIC_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20966-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKTLLIIDAN LGQARAYMAK TLLGAAARKA KLEIIDNPND AEMAIVLGDS
    60 70 80 90 100
    IPNDSALNGK NVWLGDISRA VAHPELFLSE AKGHAKPYTA PVAATAPVAA
    110 120 130 140 150
    SGPKRVVAVT ACPTGVAHTF MAAEAIETEA KKRGWWVKVE TRGSVGAGNA
    160 170 180 190 200
    ITPEEVAAAD LVIVAADIEV DLAKFAGKPM YRTSTGLALK KTAQELDKAV
    210 220 230 240 250
    AEATPYEPAG KAQTATTESK KESAGAYRHL LTGVSYMLPM VVAGGLCIAL
    260 270 280 290 300
    SFAFGIEAFK EPGTLAAALM QIGGGSAFAL MVPVLAGYIA FSIADRPGLT
    310 320 330 340 350
    PGLIGGMLAV STGSGFIGGI IAGFLAGYIA KLISTQLKLP QSMEALKPIL
    360 370 380 390 400
    IIPLISSLVV GLAMIYLIGK PVAGILEGLT HWLQTMGTAN AVLLGAILGG
    410 420 430 440 450
    MMCTDMGGPV NKAAYAFGVG LLSTQTYGPM AAIMAAGMVP PLAMGLATMV
    460 470 480 490 500
    ARRKFDKAQQ EGGKAALVLG LCFISEGAIP FAARDPMRVL PCCIVGGALT
    510 520 530 540 550
    GAISMAIGAK LMAPHGGLFV LLIPGAITPV LGYLVAIIAG TLVAGLAYAF
    560
    LKRPEVDAVA KAA
    Length:563
    Mass (Da):57,519
    Last modified:February 1, 1991 - v1
    Checksum:iAC066A96A94DBA05
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M23196 Genomic DNA. Translation: AAA62624.1.
    U00007 Genomic DNA. Translation: AAA60524.1.
    U00096 Genomic DNA. Translation: AAC75228.1.
    AP009048 Genomic DNA. Translation: BAA15976.2.
    X53948 Genomic DNA. No translation available.
    PIRiA34962.
    RefSeqiNP_416672.1. NC_000913.3.
    WP_000854447.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75228; AAC75228; b2167.
    BAA15976; BAA15976; BAA15976.
    GeneIDi946672.
    KEGGiecj:JW2154.
    eco:b2167.
    PATRICi32119683. VBIEscCol129921_2252.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M23196 Genomic DNA. Translation: AAA62624.1.
    U00007 Genomic DNA. Translation: AAA60524.1.
    U00096 Genomic DNA. Translation: AAC75228.1.
    AP009048 Genomic DNA. Translation: BAA15976.2.
    X53948 Genomic DNA. No translation available.
    PIRiA34962.
    RefSeqiNP_416672.1. NC_000913.3.
    WP_000854447.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP20966.
    SMRiP20966.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260463. 14 interactors.
    IntActiP20966. 2 interactors.
    STRINGi511145.b2167.

    Protein family/group databases

    TCDBi4.A.2.1.1. the pts fructose-mannitol (fru) family.

    Proteomic databases

    EPDiP20966.
    PaxDbiP20966.
    PRIDEiP20966.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75228; AAC75228; b2167.
    BAA15976; BAA15976; BAA15976.
    GeneIDi946672.
    KEGGiecj:JW2154.
    eco:b2167.
    PATRICi32119683. VBIEscCol129921_2252.

    Organism-specific databases

    EchoBASEiEB0332.
    EcoGeneiEG10336. fruA.

    Phylogenomic databases

    eggNOGiENOG4105DM4. Bacteria.
    COG1299. LUCA.
    COG1445. LUCA.
    COG3925. LUCA.
    HOGENOMiHOG000227677.
    InParanoidiP20966.
    KOiK02769.
    K02770.
    OMAiHAVILAC.
    PhylomeDBiP20966.

    Enzyme and pathway databases

    BioCyciEcoCyc:FRUA-MONOMER.
    ECOL316407:JW2154-MONOMER.
    MetaCyc:FRUA-MONOMER.

    Miscellaneous databases

    PROiP20966.

    Family and domain databases

    CDDicd05569. PTS_IIB_fructose. 1 hit.
    InterProiIPR013011. PTS_EIIB_2.
    IPR003501. PTS_EIIB_2/3.
    IPR003352. PTS_EIIC.
    IPR013014. PTS_EIIC_2.
    IPR003353. PTS_IIB_fruc.
    IPR006327. PTS_IIC_fruc.
    [Graphical view]
    PfamiPF02378. PTS_EIIC. 1 hit.
    PF02302. PTS_IIB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52794. SSF52794. 2 hits.
    TIGRFAMsiTIGR00829. FRU. 1 hit.
    TIGR01427. PTS_IIC_fructo. 1 hit.
    PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
    PS51104. PTS_EIIC_TYPE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPTFBC_ECOLI
    AccessioniPrimary (citable) accession number: P20966
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: November 2, 2016
    This is version 147 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.