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P20966 (PTFBC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PTS system fructose-specific EIIBC component
Alternative name(s):
EIIBC-Fru

Including the following 2 domains:

  1. Fructose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    EIII-Fru
    PTS system fructose-specific EIIB component
  2. Fructose permease IIC component
    Alternative name(s):
    PTS system fructose-specific EIIC component
Gene names
Name:fruA
Synonyms:ptsF
Ordered Locus Names:b2167, JW2154
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Ref.7.

Domain

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similarities

Contains 1 PTS EIIB type-2 domain.

Contains 1 PTS EIIC type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 563563PTS system fructose-specific EIIBC component
PRO_0000186508

Regions

Transmembrane236 – 25621Helical; Potential
Transmembrane274 – 29421Helical; Potential
Transmembrane304 – 32421Helical; Potential
Transmembrane349 – 36921Helical; Potential
Transmembrane382 – 40221Helical; Potential
Transmembrane430 – 45021Helical; Potential
Transmembrane463 – 48321Helical; Potential
Transmembrane489 – 50921Helical; Potential
Transmembrane518 – 53821Helical; Potential
Domain104 – 20198PTS EIIB type-2
Domain226 – 561336PTS EIIC type-2

Sites

Active site1121Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P20966 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: AC066A96A94DBA05

FASTA56357,519
        10         20         30         40         50         60 
MKTLLIIDAN LGQARAYMAK TLLGAAARKA KLEIIDNPND AEMAIVLGDS IPNDSALNGK 

        70         80         90        100        110        120 
NVWLGDISRA VAHPELFLSE AKGHAKPYTA PVAATAPVAA SGPKRVVAVT ACPTGVAHTF 

       130        140        150        160        170        180 
MAAEAIETEA KKRGWWVKVE TRGSVGAGNA ITPEEVAAAD LVIVAADIEV DLAKFAGKPM 

       190        200        210        220        230        240 
YRTSTGLALK KTAQELDKAV AEATPYEPAG KAQTATTESK KESAGAYRHL LTGVSYMLPM 

       250        260        270        280        290        300 
VVAGGLCIAL SFAFGIEAFK EPGTLAAALM QIGGGSAFAL MVPVLAGYIA FSIADRPGLT 

       310        320        330        340        350        360 
PGLIGGMLAV STGSGFIGGI IAGFLAGYIA KLISTQLKLP QSMEALKPIL IIPLISSLVV 

       370        380        390        400        410        420 
GLAMIYLIGK PVAGILEGLT HWLQTMGTAN AVLLGAILGG MMCTDMGGPV NKAAYAFGVG 

       430        440        450        460        470        480 
LLSTQTYGPM AAIMAAGMVP PLAMGLATMV ARRKFDKAQQ EGGKAALVLG LCFISEGAIP 

       490        500        510        520        530        540 
FAARDPMRVL PCCIVGGALT GAISMAIGAK LMAPHGGLFV LLIPGAITPV LGYLVAIIAG 

       550        560 
TLVAGLAYAF LKRPEVDAVA KAA

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of fruA, the gene specifying enzyme IIfru of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli K12."
Prior T.I., Kornberg H.L.
J. Gen. Microbiol. 134:2757-2768(1988) [PubMed: 3076173] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Automated multiplex sequencing of the E.coli genome."
Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / BHB2600.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-365.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus."
Orchard L.M.D., Kornberg H.L.
Proc. R. Soc. B 242:87-90(1990) [PubMed: 1981619] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.
Strain: K12.
[7]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M23196 Genomic DNA. Translation: AAA62624.1.
U00007 Genomic DNA. Translation: AAA60524.1.
U00096 Genomic DNA. Translation: AAC75228.1.
AP009048 Genomic DNA. Translation: BAA15976.2.
X53948 Genomic DNA. No translation available.
PIRA34962.
RefSeqNP_416672.1. NC_000913.2.

3D structure databases

ProteinModelPortalP20966.
SMRP20966. Positions 1-85, 105-207.
ModBaseSearch...

Protein-protein interaction databases

IntActP20966. 1 interaction.

Protein family/group databases

TCDB4.A.2.1.1. PTS fructose-mannitol (Fru) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000004454; EBESCP00000004454; EBESCG00000003633.
EBESCT00000016449; EBESCP00000015740; EBESCG00000015509.
GeneID946672.
GenomeReviewsGene locus JW2154 in contig AP009048_GR.
Gene locus b2167 in contig U00096_GR.
KEGGecj:JW2154.
eco:b2167.
PATRIC32119683. VBIEscCol129921_2252.

Organism-specific databases

EchoBASEEB0332.
EcoGeneEG10336. fruA.

Phylogenomic databases

eggNOGCOG1299.
GeneTreeEBGT00070000031702.
HOGENOMHBG704192.
OMAINHALLY.
PhylomeDBP20966.
ProtClustDBPRK10712.

Enzyme and pathway databases

BioCycEcoCyc:FRUA-MONOMER.

Gene expression databases

GenevestigatorP20966.

Family and domain databases

InterProIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
KOK02769.
K02770.
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00829. FRU. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTFBC_ECOLI
AccessionPrimary (citable) accession number: P20966
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 25, 2012
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents