Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PTS system fructose-specific EIIBC component

Gene

fruA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei112 – 1121Phosphocysteine intermediate; for EIIB activityBy similarity

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transmembrane transport Source: GOC
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:FRUA-MONOMER.
ECOL316407:JW2154-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.1. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system fructose-specific EIIBC component
Alternative name(s):
EIIBC-Fru
Including the following 2 domains:
Fructose-specific phosphotransferase enzyme IIB component (EC:2.7.1.202)
Alternative name(s):
EIII-Fru
PTS system fructose-specific EIIB component
Fructose permease IIC component
Alternative name(s):
PTS system fructose-specific EIIC component
Gene namesi
Name:fruA
Synonyms:ptsF
Ordered Locus Names:b2167, JW2154
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10336. fruA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei236 – 25621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei274 – 29421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei304 – 32421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei349 – 36921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei382 – 40221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei430 – 45021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei463 – 48321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei489 – 50921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei518 – 53821HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 563563PTS system fructose-specific EIIBC componentPRO_0000186508Add
BLAST

Proteomic databases

EPDiP20966.
PaxDbiP20966.
PRIDEiP20966.

Interactioni

Protein-protein interaction databases

BioGridi4260463. 14 interactions.
IntActiP20966. 2 interactions.
STRINGi511145.b2167.

Structurei

3D structure databases

ProteinModelPortaliP20966.
SMRiP20966. Positions 105-205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 20198PTS EIIB type-2PROSITE-ProRule annotationAdd
BLAST
Domaini226 – 561336PTS EIIC type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
COG3925. LUCA.
HOGENOMiHOG000227677.
InParanoidiP20966.
KOiK02769.
K02770.
OMAiHAVILAC.
OrthoDBiEOG6XDGX2.
PhylomeDBiP20966.

Family and domain databases

InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 2 hits.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLLIIDAN LGQARAYMAK TLLGAAARKA KLEIIDNPND AEMAIVLGDS
60 70 80 90 100
IPNDSALNGK NVWLGDISRA VAHPELFLSE AKGHAKPYTA PVAATAPVAA
110 120 130 140 150
SGPKRVVAVT ACPTGVAHTF MAAEAIETEA KKRGWWVKVE TRGSVGAGNA
160 170 180 190 200
ITPEEVAAAD LVIVAADIEV DLAKFAGKPM YRTSTGLALK KTAQELDKAV
210 220 230 240 250
AEATPYEPAG KAQTATTESK KESAGAYRHL LTGVSYMLPM VVAGGLCIAL
260 270 280 290 300
SFAFGIEAFK EPGTLAAALM QIGGGSAFAL MVPVLAGYIA FSIADRPGLT
310 320 330 340 350
PGLIGGMLAV STGSGFIGGI IAGFLAGYIA KLISTQLKLP QSMEALKPIL
360 370 380 390 400
IIPLISSLVV GLAMIYLIGK PVAGILEGLT HWLQTMGTAN AVLLGAILGG
410 420 430 440 450
MMCTDMGGPV NKAAYAFGVG LLSTQTYGPM AAIMAAGMVP PLAMGLATMV
460 470 480 490 500
ARRKFDKAQQ EGGKAALVLG LCFISEGAIP FAARDPMRVL PCCIVGGALT
510 520 530 540 550
GAISMAIGAK LMAPHGGLFV LLIPGAITPV LGYLVAIIAG TLVAGLAYAF
560
LKRPEVDAVA KAA
Length:563
Mass (Da):57,519
Last modified:February 1, 1991 - v1
Checksum:iAC066A96A94DBA05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23196 Genomic DNA. Translation: AAA62624.1.
U00007 Genomic DNA. Translation: AAA60524.1.
U00096 Genomic DNA. Translation: AAC75228.1.
AP009048 Genomic DNA. Translation: BAA15976.2.
X53948 Genomic DNA. No translation available.
PIRiA34962.
RefSeqiNP_416672.1. NC_000913.3.
WP_000854447.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75228; AAC75228; b2167.
BAA15976; BAA15976; BAA15976.
GeneIDi946672.
KEGGiecj:JW2154.
eco:b2167.
PATRICi32119683. VBIEscCol129921_2252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23196 Genomic DNA. Translation: AAA62624.1.
U00007 Genomic DNA. Translation: AAA60524.1.
U00096 Genomic DNA. Translation: AAC75228.1.
AP009048 Genomic DNA. Translation: BAA15976.2.
X53948 Genomic DNA. No translation available.
PIRiA34962.
RefSeqiNP_416672.1. NC_000913.3.
WP_000854447.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP20966.
SMRiP20966. Positions 105-205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260463. 14 interactions.
IntActiP20966. 2 interactions.
STRINGi511145.b2167.

Protein family/group databases

TCDBi4.A.2.1.1. the pts fructose-mannitol (fru) family.

Proteomic databases

EPDiP20966.
PaxDbiP20966.
PRIDEiP20966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75228; AAC75228; b2167.
BAA15976; BAA15976; BAA15976.
GeneIDi946672.
KEGGiecj:JW2154.
eco:b2167.
PATRICi32119683. VBIEscCol129921_2252.

Organism-specific databases

EchoBASEiEB0332.
EcoGeneiEG10336. fruA.

Phylogenomic databases

eggNOGiENOG4105DM4. Bacteria.
COG1299. LUCA.
COG1445. LUCA.
COG3925. LUCA.
HOGENOMiHOG000227677.
InParanoidiP20966.
KOiK02769.
K02770.
OMAiHAVILAC.
OrthoDBiEOG6XDGX2.
PhylomeDBiP20966.

Enzyme and pathway databases

BioCyciEcoCyc:FRUA-MONOMER.
ECOL316407:JW2154-MONOMER.

Miscellaneous databases

PROiP20966.

Family and domain databases

InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 2 hits.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of fruA, the gene specifying enzyme IIfru of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli K12."
    Prior T.I., Kornberg H.L.
    J. Gen. Microbiol. 134:2757-2768(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-365.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus."
    Orchard L.M.D., Kornberg H.L.
    Proc. R. Soc. B 242:87-90(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.
    Strain: K12.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPTFBC_ECOLI
AccessioniPrimary (citable) accession number: P20966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 11, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.