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P20961 (PAI1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasminogen activator inhibitor 1

Short name=PAI
Short name=PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene names
Name:Serpine1
Synonyms:Pai1, Planh1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length402 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis By similarity.

Subunit structure

Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the serpin family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to gravity

Inferred from expression pattern PubMed 19574431. Source: RGD

glucose homeostasis

Inferred from expression pattern PubMed 12105191. Source: RGD

negative regulation of endopeptidase activity

Inferred by curator PubMed 8626514. Source: BHF-UCL

negative regulation of proteolysis

Traceable author statement PubMed 17071586. Source: RGD

positive regulation of coagulation

Inferred from mutant phenotype PubMed 18239154. Source: RGD

positive regulation of receptor-mediated endocytosis

Inferred from mutant phenotype PubMed 8626514. Source: BHF-UCL

regulation of proteolysis

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to cytokine

Inferred from expression pattern PubMed 20083474. Source: RGD

response to estrogen

Inferred from expression pattern PubMed 20838740. Source: RGD

response to glucocorticoid

Inferred from expression pattern. Source: RGD

response to hyperoxia

Inferred from expression pattern PubMed 19099788. Source: RGD

response to reactive oxygen species

Inferred from expression pattern PubMed 12719791. Source: RGD

tissue regeneration

Inferred from expression pattern PubMed 12399226. Source: RGD

wound healing

Inferred from mutant phenotype PubMed 17071586. Source: RGD

   Cellular_componentextracellular matrix

Inferred from direct assay PubMed 17071586. Source: RGD

extracellular space

Inferred from direct assay PubMed 20083474. Source: RGD

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 402379Plasminogen activator inhibitor 1
PRO_0000032503

Sites

Site369 – 3702Reactive bond

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation3521N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P20961 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 2B639140F475EB53

FASTA40245,010
        10         20         30         40         50         60 
MQMSSALTCL TLGLVLVFGK GFASPLPESH TAQQATNFGV KVFQHVVQAS KDRNVVFSPY 

        70         80         90        100        110        120 
GVSSVLAMLQ LTTAGKTRQQ IQDAMGFNIS ERGTAPALRK LSKELMGSWN KNEISTADAI 

       130        140        150        160        170        180 
FVQRDLELVQ GFMPHFFKLF RTTVKQVDFS EVERARFIIN DWVERHTKGM ISDLLAKGAV 

       190        200        210        220        230        240 
NELTRLVLVN ALYFNGQWKT PFLEASTHQR LFHKSDGSTI SVPMMAQNNK FNYTEFTTPD 

       250        260        270        280        290        300 
GHEYDILELP YHGETLSMFI AAPFEKDVPL SAITNILDAE LIRQWKSNMT RLPRLLILPK 

       310        320        330        340        350        360 
FSLETEVDLR GPLEKLGMTD IFSSTQADFT SLSDQEQLSV AQALQKVKIE VNESGTVASS 

       370        380        390        400 
STAILVSARM APTEMVLDRS FLFVVRHNPT ETILFMGQLM EP 

« Hide

References

[1]"Isolation and characterization of the rat plasminogen activator inhibitor-1 gene."
Bruzdzinski C.J., Riordan-Johnson M., Nordby E.C., Suter S.M., Gelehrter T.D.
J. Biol. Chem. 265:2078-2085(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning and sequencing of cDNA for the rat plasminogen activator inhibitor-1."
Zeheb R., Gelehrter T.D.
Gene 73:459-468(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05206 Genomic DNA. Translation: AAA41796.1.
M24067 mRNA. Translation: AAA56856.1.
PIRA35032.
RefSeqNP_036752.1. NM_012620.1.
UniGeneRn.29367.

3D structure databases

ProteinModelPortalP20961.
SMRP20961. Positions 27-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246756. 2 interactions.
STRING10116.ENSRNOP00000001916.

Chemistry

BindingDBP20961.
ChEMBLCHEMBL2155.

Protein family/group databases

MEROPSI04.020.

Proteomic databases

PaxDbP20961.
PRIDEP20961.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24617.
KEGGrno:24617.
UCSCRGD:3249. rat.

Organism-specific databases

CTD5054.
RGD3249. Serpine1.

Phylogenomic databases

eggNOGCOG4826.
HOGENOMHOG000238519.
HOVERGENHBG106493.
InParanoidP20961.
KOK03982.
PhylomeDBP20961.

Gene expression databases

GenevestigatorP20961.

Family and domain databases

InterProIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603870.
PROP20961.

Entry information

Entry namePAI1_RAT
AccessionPrimary (citable) accession number: P20961
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families