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Protein

Plasminogen activator inhibitor 1

Gene

Serpine1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei369 – 3702Reactive bond

GO - Molecular functioni

  • protease binding Source: RGD
  • serine-type endopeptidase inhibitor activity Source: RGD

GO - Biological processi

  • cellular response to ATP Source: RGD
  • cellular response to cAMP Source: RGD
  • cellular response to cGMP Source: RGD
  • cellular response to cycloheximide Source: RGD
  • cellular response to dexamethasone stimulus Source: RGD
  • cellular response to drug Source: RGD
  • cellular response to fibroblast growth factor stimulus Source: RGD
  • cellular response to follicle-stimulating hormone stimulus Source: RGD
  • cellular response to glucagon stimulus Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to gravity Source: RGD
  • cellular response to growth factor stimulus Source: RGD
  • cellular response to hydrogen peroxide Source: RGD
  • cellular response to hypoxia Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • cellular response to interleukin-1 Source: RGD
  • cellular response to low-density lipoprotein particle stimulus Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • cellular response to metal ion Source: RGD
  • cellular response to nerve growth factor stimulus Source: RGD
  • cellular response to nicotine Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • cellular response to platelet-derived growth factor stimulus Source: RGD
  • cellular response to potassium ion Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • decidualization Source: RGD
  • female gonad development Source: RGD
  • glucose homeostasis Source: RGD
  • mast cell activation Source: RGD
  • negative regulation of cell migration Source: RGD
  • negative regulation of endopeptidase activity Source: BHF-UCL
  • negative regulation of gene expression Source: RGD
  • negative regulation of proteolysis Source: RGD
  • positive regulation of calcium ion import Source: RGD
  • positive regulation of coagulation Source: RGD
  • positive regulation of fibroblast proliferation Source: RGD
  • positive regulation of gene expression Source: RGD
  • positive regulation of keratinocyte migration Source: RGD
  • positive regulation of receptor-mediated endocytosis Source: BHF-UCL
  • response to cytokine Source: RGD
  • response to epidermal growth factor Source: RGD
  • response to estrogen Source: RGD
  • response to hyperoxia Source: RGD
  • response to laminar fluid shear stress Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to reactive oxygen species Source: RGD
  • response to starvation Source: RGD
  • response to transforming growth factor beta Source: RGD
  • tissue regeneration Source: RGD
  • wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen activator inhibitor 1
Short name:
PAI
Short name:
PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene namesi
Name:Serpine1
Synonyms:Pai1, Planh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3249. Serpine1.

Subcellular locationi

GO - Cellular componenti

  • chromaffin granule Source: RGD
  • extracellular matrix Source: RGD
  • extracellular region Source: RGD
  • extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 402379Plasminogen activator inhibitor 1PRO_0000032503Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP20961.
PRIDEiP20961.

Expressioni

Gene expression databases

GenevisibleiP20961. RN.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation (By similarity).By similarity

Protein-protein interaction databases

BioGridi246756. 2 interactions.
STRINGi10116.ENSRNOP00000001916.

Structurei

3D structure databases

ProteinModelPortaliP20961.
SMRiP20961. Positions 27-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP20961.
KOiK03982.
PhylomeDBiP20961.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQMSSALTCL TLGLVLVFGK GFASPLPESH TAQQATNFGV KVFQHVVQAS
60 70 80 90 100
KDRNVVFSPY GVSSVLAMLQ LTTAGKTRQQ IQDAMGFNIS ERGTAPALRK
110 120 130 140 150
LSKELMGSWN KNEISTADAI FVQRDLELVQ GFMPHFFKLF RTTVKQVDFS
160 170 180 190 200
EVERARFIIN DWVERHTKGM ISDLLAKGAV NELTRLVLVN ALYFNGQWKT
210 220 230 240 250
PFLEASTHQR LFHKSDGSTI SVPMMAQNNK FNYTEFTTPD GHEYDILELP
260 270 280 290 300
YHGETLSMFI AAPFEKDVPL SAITNILDAE LIRQWKSNMT RLPRLLILPK
310 320 330 340 350
FSLETEVDLR GPLEKLGMTD IFSSTQADFT SLSDQEQLSV AQALQKVKIE
360 370 380 390 400
VNESGTVASS STAILVSARM APTEMVLDRS FLFVVRHNPT ETILFMGQLM

EP
Length:402
Mass (Da):45,010
Last modified:February 1, 1991 - v1
Checksum:i2B639140F475EB53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05206 Genomic DNA. Translation: AAA41796.1.
M24067 mRNA. Translation: AAA56856.1.
PIRiA35032.
RefSeqiNP_036752.1. NM_012620.1.
UniGeneiRn.29367.

Genome annotation databases

GeneIDi24617.
KEGGirno:24617.
UCSCiRGD:3249. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05206 Genomic DNA. Translation: AAA41796.1.
M24067 mRNA. Translation: AAA56856.1.
PIRiA35032.
RefSeqiNP_036752.1. NM_012620.1.
UniGeneiRn.29367.

3D structure databases

ProteinModelPortaliP20961.
SMRiP20961. Positions 27-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246756. 2 interactions.
STRINGi10116.ENSRNOP00000001916.

Chemistry

BindingDBiP20961.
ChEMBLiCHEMBL2155.

Protein family/group databases

MEROPSiI04.020.

Proteomic databases

PaxDbiP20961.
PRIDEiP20961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24617.
KEGGirno:24617.
UCSCiRGD:3249. rat.

Organism-specific databases

CTDi5054.
RGDi3249. Serpine1.

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP20961.
KOiK03982.
PhylomeDBiP20961.

Miscellaneous databases

NextBioi603870.
PROiP20961.

Gene expression databases

GenevisibleiP20961. RN.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of the rat plasminogen activator inhibitor-1 gene."
    Bruzdzinski C.J., Riordan-Johnson M., Nordby E.C., Suter S.M., Gelehrter T.D.
    J. Biol. Chem. 265:2078-2085(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and sequencing of cDNA for the rat plasminogen activator inhibitor-1."
    Zeheb R., Gelehrter T.D.
    Gene 73:459-468(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPAI1_RAT
AccessioniPrimary (citable) accession number: P20961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 24, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.