P20961 (PAI1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Plasminogen activator inhibitor 1
Short name=PAI
Short name=PAI-1

Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1

Gene names

Name:	Serpine1
Synonyms:	Pai1, Planh1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis By similarity.
Subunit structure	Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the serpin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to gravity Inferred from expression pattern PubMed 19574431. Source: RGD glucose homeostasis Inferred from expression pattern PubMed 12105191. Source: RGD negative regulation of proteolysis Traceable author statement PubMed 17071586. Source: RGD positive regulation of coagulation Inferred from mutant phenotype PubMed 18239154. Source: RGD positive regulation of receptor-mediated endocytosis Inferred from mutant phenotype PubMed 8626514. Source: BHF-UCL response to cytokine stimulus Inferred from expression pattern PubMed 20083474. Source: RGD response to estrogen stimulus Inferred from expression pattern PubMed 20838740. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern PubMed 17474984. Source: RGD response to hyperoxia Inferred from expression pattern PubMed 19099788. Source: RGD response to reactive oxygen species Inferred from expression pattern PubMed 12719791. Source: RGD tissue regeneration Inferred from expression pattern PubMed 12399226. Source: RGD
Cellular_component	extracellular matrix Inferred from direct assay PubMed 17071586. Source: RGD extracellular space Inferred from direct assay PubMed 20083474. Source: RGD
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 402	379	Plasminogen activator inhibitor 1		PRO_0000032503
Sites
	Site	369 – 370	2	Reactive bond
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	232	1	N-linked (GlcNAc...) Potential
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
	Glycosylation	352	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P20961 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 2B639140F475EB53	FASTA	402	45,010
	10 20 30 40 50 60 MQMSSALTCL TLGLVLVFGK GFASPLPESH TAQQATNFGV KVFQHVVQAS KDRNVVFSPY 70 80 90 100 110 120 GVSSVLAMLQ LTTAGKTRQQ IQDAMGFNIS ERGTAPALRK LSKELMGSWN KNEISTADAI 130 140 150 160 170 180 FVQRDLELVQ GFMPHFFKLF RTTVKQVDFS EVERARFIIN DWVERHTKGM ISDLLAKGAV 190 200 210 220 230 240 NELTRLVLVN ALYFNGQWKT PFLEASTHQR LFHKSDGSTI SVPMMAQNNK FNYTEFTTPD 250 260 270 280 290 300 GHEYDILELP YHGETLSMFI AAPFEKDVPL SAITNILDAE LIRQWKSNMT RLPRLLILPK 310 320 330 340 350 360 FSLETEVDLR GPLEKLGMTD IFSSTQADFT SLSDQEQLSV AQALQKVKIE VNESGTVASS 370 380 390 400 STAILVSARM APTEMVLDRS FLFVVRHNPT ETILFMGQLM EP

References

[1]	"Isolation and characterization of the rat plasminogen activator inhibitor-1 gene." Bruzdzinski C.J., Riordan-Johnson M., Nordby E.C., Suter S.M., Gelehrter T.D. J. Biol. Chem. 265:2078-2085(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and sequencing of cDNA for the rat plasminogen activator inhibitor-1." Zeheb R., Gelehrter T.D. Gene 73:459-468(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05206 Genomic DNA. Translation: AAA41796.1. M24067 mRNA. Translation: AAA56856.1.
IPI	IPI00204483.
PIR	A35032.
RefSeq	NP_036752.1. NM_012620.1.
UniGene	Rn.29367.
3D structure databases
ProteinModelPortal	P20961.
SMR	P20961. Positions 27-402.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000001916.
Protein family/group databases
MEROPS	I04.020.
Proteomic databases
PaxDb	P20961.
PRIDE	P20961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24617.
KEGG	rno:24617.
UCSC	RGD:3249. rat.
Organism-specific databases
CTD	5054.
RGD	3249. Serpine1.
Phylogenomic databases
eggNOG	COG4826.
HOGENOM	HOG000238519.
HOVERGEN	HBG106493.
InParanoid	P20961.
KO	K03982.
OrthoDB	EOG4X3H1K.
Gene expression databases
ArrayExpress	P20961.
Genevestigator	P20961.
GermOnline	ENSRNOG00000001414. Rattus norvegicus.
Family and domain databases
InterPro	IPR023795. Protease_inhib_I4_serpin_CS. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P20961.
ChEMBL	CHEMBL2155.
NextBio	603870.

Entry information

Entry name

PAI1_RAT

Accession

Primary (citable) accession number: P20961

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program