Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Plasminogen activator inhibitor 1

Gene

Serpine1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei369 – 3702Reactive bond

GO - Molecular functioni

  1. protease binding Source: RGD
  2. serine-type endopeptidase inhibitor activity Source: RGD

GO - Biological processi

  1. cellular response to ATP Source: RGD
  2. cellular response to cAMP Source: RGD
  3. cellular response to cGMP Source: RGD
  4. cellular response to cycloheximide Source: RGD
  5. cellular response to dexamethasone stimulus Source: RGD
  6. cellular response to drug Source: RGD
  7. cellular response to fibroblast growth factor stimulus Source: RGD
  8. cellular response to follicle-stimulating hormone stimulus Source: RGD
  9. cellular response to glucagon stimulus Source: RGD
  10. cellular response to glucose stimulus Source: RGD
  11. cellular response to gravity Source: RGD
  12. cellular response to growth factor stimulus Source: RGD
  13. cellular response to hydrogen peroxide Source: RGD
  14. cellular response to hypoxia Source: RGD
  15. cellular response to insulin stimulus Source: RGD
  16. cellular response to interleukin-1 Source: RGD
  17. cellular response to low-density lipoprotein particle stimulus Source: RGD
  18. cellular response to mechanical stimulus Source: RGD
  19. cellular response to metal ion Source: RGD
  20. cellular response to nerve growth factor stimulus Source: RGD
  21. cellular response to nicotine Source: RGD
  22. cellular response to organic cyclic compound Source: RGD
  23. cellular response to platelet-derived growth factor stimulus Source: RGD
  24. cellular response to potassium ion Source: RGD
  25. cellular response to transforming growth factor beta stimulus Source: RGD
  26. decidualization Source: RGD
  27. female gonad development Source: RGD
  28. glucose homeostasis Source: RGD
  29. mast cell activation Source: RGD
  30. negative regulation of cell migration Source: RGD
  31. negative regulation of endopeptidase activity Source: BHF-UCL
  32. negative regulation of gene expression Source: RGD
  33. negative regulation of proteolysis Source: RGD
  34. positive regulation of calcium ion import Source: RGD
  35. positive regulation of coagulation Source: RGD
  36. positive regulation of fibroblast proliferation Source: RGD
  37. positive regulation of gene expression Source: RGD
  38. positive regulation of keratinocyte migration Source: RGD
  39. positive regulation of receptor-mediated endocytosis Source: BHF-UCL
  40. regulation of proteolysis Source: GO_Central
  41. response to cytokine Source: RGD
  42. response to epidermal growth factor Source: RGD
  43. response to estrogen Source: RGD
  44. response to hyperoxia Source: RGD
  45. response to laminar fluid shear stress Source: RGD
  46. response to lipopolysaccharide Source: RGD
  47. response to reactive oxygen species Source: RGD
  48. response to starvation Source: RGD
  49. response to transforming growth factor beta Source: RGD
  50. tissue regeneration Source: RGD
  51. wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen activator inhibitor 1
Short name:
PAI
Short name:
PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene namesi
Name:Serpine1
Synonyms:Pai1, Planh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3249. Serpine1.

Subcellular locationi

GO - Cellular componenti

  1. chromaffin granule Source: RGD
  2. extracellular matrix Source: RGD
  3. extracellular region Source: RGD
  4. extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 402379Plasminogen activator inhibitor 1PRO_0000032503Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP20961.
PRIDEiP20961.

Expressioni

Gene expression databases

GenevestigatoriP20961.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation (By similarity).By similarity

Protein-protein interaction databases

BioGridi246756. 2 interactions.
STRINGi10116.ENSRNOP00000001916.

Structurei

3D structure databases

ProteinModelPortaliP20961.
SMRiP20961. Positions 27-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP20961.
KOiK03982.
PhylomeDBiP20961.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20961-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQMSSALTCL TLGLVLVFGK GFASPLPESH TAQQATNFGV KVFQHVVQAS
60 70 80 90 100
KDRNVVFSPY GVSSVLAMLQ LTTAGKTRQQ IQDAMGFNIS ERGTAPALRK
110 120 130 140 150
LSKELMGSWN KNEISTADAI FVQRDLELVQ GFMPHFFKLF RTTVKQVDFS
160 170 180 190 200
EVERARFIIN DWVERHTKGM ISDLLAKGAV NELTRLVLVN ALYFNGQWKT
210 220 230 240 250
PFLEASTHQR LFHKSDGSTI SVPMMAQNNK FNYTEFTTPD GHEYDILELP
260 270 280 290 300
YHGETLSMFI AAPFEKDVPL SAITNILDAE LIRQWKSNMT RLPRLLILPK
310 320 330 340 350
FSLETEVDLR GPLEKLGMTD IFSSTQADFT SLSDQEQLSV AQALQKVKIE
360 370 380 390 400
VNESGTVASS STAILVSARM APTEMVLDRS FLFVVRHNPT ETILFMGQLM

EP
Length:402
Mass (Da):45,010
Last modified:February 1, 1991 - v1
Checksum:i2B639140F475EB53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05206 Genomic DNA. Translation: AAA41796.1.
M24067 mRNA. Translation: AAA56856.1.
PIRiA35032.
RefSeqiNP_036752.1. NM_012620.1.
UniGeneiRn.29367.

Genome annotation databases

GeneIDi24617.
KEGGirno:24617.
UCSCiRGD:3249. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05206 Genomic DNA. Translation: AAA41796.1.
M24067 mRNA. Translation: AAA56856.1.
PIRiA35032.
RefSeqiNP_036752.1. NM_012620.1.
UniGeneiRn.29367.

3D structure databases

ProteinModelPortaliP20961.
SMRiP20961. Positions 27-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246756. 2 interactions.
STRINGi10116.ENSRNOP00000001916.

Chemistry

BindingDBiP20961.
ChEMBLiCHEMBL2155.

Protein family/group databases

MEROPSiI04.020.

Proteomic databases

PaxDbiP20961.
PRIDEiP20961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24617.
KEGGirno:24617.
UCSCiRGD:3249. rat.

Organism-specific databases

CTDi5054.
RGDi3249. Serpine1.

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP20961.
KOiK03982.
PhylomeDBiP20961.

Miscellaneous databases

NextBioi603870.
PROiP20961.

Gene expression databases

GenevestigatoriP20961.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of the rat plasminogen activator inhibitor-1 gene."
    Bruzdzinski C.J., Riordan-Johnson M., Nordby E.C., Suter S.M., Gelehrter T.D.
    J. Biol. Chem. 265:2078-2085(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and sequencing of cDNA for the rat plasminogen activator inhibitor-1."
    Zeheb R., Gelehrter T.D.
    Gene 73:459-468(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPAI1_RAT
AccessioniPrimary (citable) accession number: P20961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: February 4, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.