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Protein

Insulin-like growth factor-binding protein 3

Gene

IGFBP3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Growth factor binding

Enzyme and pathway databases

ReactomeiR-BTA-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-BTA-6803211. TP53 Regulates Transcription of Death Receptors and Ligands.

Protein family/group databases

MEROPSiI31.952.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor-binding protein 3
Short name:
IBP-3
Short name:
IGF-binding protein 3
Short name:
IGFBP-3
Gene namesi
Name:IGFBP3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 4

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 291264Insulin-like growth factor-binding protein 3PRO_0000014377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence analysis
Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence analysis
Modified residuei148 – 1481PhosphoserineBy similarity
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence analysis
Modified residuei201 – 2011PhosphoserineBy similarity
Disulfide bondi213 ↔ 240PROSITE-ProRule annotation
Disulfide bondi251 ↔ 262PROSITE-ProRule annotation
Disulfide bondi264 ↔ 285PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by FAM20C in the extracellular medium.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP20959.
PRIDEiP20959.

Expressioni

Tissue specificityi

Plasma; expressed by most tissues.

Gene expression databases

ExpressionAtlasiP20959. baseline and differential.

Interactioni

Subunit structurei

Interacts with HN. Interacts with TMEM219 (By similarity). Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with XLKD1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000047769.

Structurei

3D structure databases

ProteinModelPortaliP20959.
SMRiP20959. Positions 38-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 11984IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini210 – 28576Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni28 – 134107IGF-bindingSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi138 – 16124Ser/Thr-richAdd
BLAST
Compositional biasi192 – 20817Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHZU. Eukaryota.
ENOG4111MF3. LUCA.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP20959.
KOiK10138.
OMAiRALEQCK.
OrthoDBiEOG74N5HG.
TreeFamiTF331211.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20959-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRARPALWA AALTALTLLR GPPAARAGAG TMGAGPVVRC EPCDARAVAQ
60 70 80 90 100
CAPPPPSPPC AELVREPGCG CCLTCALREG QPCGVYTERC GSGLRCQPPP
110 120 130 140 150
GDPRPLQALL DGRGLCANAS AVGRLRPYLL PSASGNGSES EEDHSMGSTE
160 170 180 190 200
NQAGPSTHRV PVSKFHPIHT KMDVIKKGHA KDSQRYKVDY ESQSTDTQNF
210 220 230 240 250
SSESKRETEY GPCRREMEDT LNHLKFLNML SPRGIHIPNC DKKGFYKKKQ
260 270 280 290
CRPSKGRKRG FCWCVDKYGQ PLPGFDVKGK GDVHCYSMES K
Length:291
Mass (Da):31,570
Last modified:February 7, 2006 - v3
Checksum:i19DEC2F09528AE12
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 73RPA → PPR in AAA30582 (PubMed:1711841).Curated
Sequence conflicti66 – 672EP → DA in AAA30582 (PubMed:1711841).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76478 mRNA. Translation: AAA30582.1.
AF305199 mRNA. Translation: AAG29824.1.
AF305712 Genomic DNA. Translation: AAG29825.1.
BC149336 mRNA. Translation: AAI49337.1.
U83465 Genomic DNA. Translation: AAB41430.1.
PIRiJN0064.
RefSeqiNP_776981.1. NM_174556.1.
UniGeneiBt.64897.

Genome annotation databases

EnsembliENSBTAT00000053426; ENSBTAP00000047769; ENSBTAG00000003994.
GeneIDi282261.
KEGGibta:282261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76478 mRNA. Translation: AAA30582.1.
AF305199 mRNA. Translation: AAG29824.1.
AF305712 Genomic DNA. Translation: AAG29825.1.
BC149336 mRNA. Translation: AAI49337.1.
U83465 Genomic DNA. Translation: AAB41430.1.
PIRiJN0064.
RefSeqiNP_776981.1. NM_174556.1.
UniGeneiBt.64897.

3D structure databases

ProteinModelPortaliP20959.
SMRiP20959. Positions 38-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000047769.

Protein family/group databases

MEROPSiI31.952.

Proteomic databases

PaxDbiP20959.
PRIDEiP20959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000053426; ENSBTAP00000047769; ENSBTAG00000003994.
GeneIDi282261.
KEGGibta:282261.

Organism-specific databases

CTDi3486.

Phylogenomic databases

eggNOGiENOG410IHZU. Eukaryota.
ENOG4111MF3. LUCA.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP20959.
KOiK10138.
OMAiRALEQCK.
OrthoDBiEOG74N5HG.
TreeFamiTF331211.

Enzyme and pathway databases

ReactomeiR-BTA-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-BTA-6803211. TP53 Regulates Transcription of Death Receptors and Ligands.

Gene expression databases

ExpressionAtlasiP20959. baseline and differential.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of bovine insulin-like growth factor binding protein-3 (bIGFBP-3)."
    Spratt S.K., Tatsuno G.P., Sommer A.
    Biochem. Biophys. Res. Commun. 177:1025-1032(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Bovine insulin-like growth factor binding protein-3: organization of the chromosomal gene and functional analysis of its promoter."
    Erondu N.E., Toland B., Boes M., Dake B., Moser D.R., Bar R.S.
    Endocrinology 138:2856-2862(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Liver.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal lung.
  4. "Structural and biological characterization of bovine insulin-like growth factor binding protein-3."
    Conover C.A., Ronk M., Lombana F., Powell D.R.
    Endocrinology 127:2795-2803(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-52.
  5. "A novel polymorphism in the bovine insulin-like growth factor binding protein-3 (IGFBP3) gene."
    Maciulla J.H., Zhang H.M., Denise S.K.
    Anim. Genet. 28:375-375(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 194-266.
  6. "Cloning, expression, characterization and role in autocrine cell growth of cell surface retention sequence binding protein-1."
    Huang S.S., Tang F.-M., Huang Y.-H., Liu I.-H., Hsu S.-C., Chen S.-T., Huang J.S.
    J. Biol. Chem. 278:43855-43869(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH XLKD1.

Entry informationi

Entry nameiIBP3_BOVIN
AccessioniPrimary (citable) accession number: P20959
Secondary accession number(s): A6QPI3, Q9GJV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 7, 2006
Last modified: June 8, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.