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Protein

Protein Tat

Gene

tat

Organism
Equine infectious anemia virus (strain Wyoming) (EIAV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to enhance transcription by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a branched hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA). The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (Probable).1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Host-virus interaction, Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Tat
Alternative name(s):
E-Tat
Transactivating regulatory protein
eTat
Gene namesi
Name:tat
OrganismiEquine infectious anemia virus (strain Wyoming) (EIAV)
Taxonomic identifieri11672 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusEquine lentivirus group
Virus hostiEquus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000854881 – 75Protein TatAdd BLAST75

Interactioni

Subunit structurei

Binds to equine CCNT1. Participates in the formation of a complex composed at least of Tat, P-TEFb, TAR RNA, RNA Pol II (Probable).Curated

Structurei

Secondary structure

175
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni52 – 57Combined sources6
Helixi58 – 62Combined sources5
Turni66 – 68Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W2HX-ray3.25C/D47-75[»]
ProteinModelPortaliP20920.
SMRiP20920.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20920.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 49CoreBy similarityAdd BLAST15
Regioni55 – 75RNA-binding (TAR)Add BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi55 – 63Nuclear localization signal9

Sequence similaritiesi

Belongs to the lentiviruses Tat family.Curated

Family and domain databases

InterProiIPR001831. IV_Tat.
[Graphical view]
PfamiPF00539. Tat. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRRIPGTA EENLQKSSGG VPGQNTGGQE ARPNYHCQLC FLRSLGIDYL
60 70
DASLRKKNKQ RLKAIQQGRQ PQYLL
Length:75
Mass (Da):8,414
Last modified:January 23, 2007 - v3
Checksum:iF5AD0FCF2D23FBCE
GO

Sequence cautioni

The sequence AAA43025 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36592 Genomic RNA. Translation: AAB02403.2.
M54797 Genomic RNA. Translation: AAA43025.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36592 Genomic RNA. Translation: AAB02403.2.
M54797 Genomic RNA. Translation: AAA43025.1. Sequence problems.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W2HX-ray3.25C/D47-75[»]
ProteinModelPortaliP20920.
SMRiP20920.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP20920.

Family and domain databases

InterProiIPR001831. IV_Tat.
[Graphical view]
PfamiPF00539. Tat. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAT_EIAVY
AccessioniPrimary (citable) accession number: P20920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.