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Protein

Protein Rev

Gene

rev

Organism
Equine infectious anemia virus (strain Wyoming) (EIAV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry two recognition sequences that function as Rev responsive element (RRE), that are not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Rev
Alternative name(s):
3'-ORF protein
Gene namesi
Name:rev
OrganismiEquine infectious anemia virus (strain Wyoming) (EIAV)
Taxonomic identifieri11672 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusEquine lentivirus group
Virus hostiEquus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi159K → A: Complete loss of nuclear import; when associated with A-160. 1 Publication1
Mutagenesisi160R → A: Complete loss of nuclear import; when associated with A-159 or A-161. 1 Publication1
Mutagenesisi161R → A: Complete loss of nuclear import; when associated with A-160 or A-162. 1 Publication1
Mutagenesisi162R → A: Complete loss of nuclear import; when associated with A-161 or A-163. 1 Publication1
Mutagenesisi163K → A: Complete loss of nuclear import; when associated with A-162. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000854781 – 165Protein RevAdd BLAST165

Interactioni

Subunit structurei

Homomultimer; when bound to the RRE. Multimeric assembly is essential for activity (By similarity).By similarity

Protein-protein interaction databases

ELMiP20919.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 130RNA-binding (RRE)Add BLAST74
Regioni144 – 165RNA-binding (RRE)Add BLAST22

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 26Sequence analysisAdd BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 55Nuclear export signalCuratedAdd BLAST24
Motifi159 – 163Nuclear localization signal5

Domaini

The bipartite RNA-binding motif binds to the RREs present in incompletely spliced viral pre-mRNAs. It consists of a central region, and a C-terminal region that also contains the NLS which mediates nuclear localization. These overlapping functions prevent Rev bound to RRE from undesirable return to the nucleus. When Rev binds the RRE, the NLS becomes masked while the NES remains accessible.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiView protein in InterPro
IPR021311. EIAV_Rev.
IPR001361. Gp90_EIAV.
PfamiView protein in Pfam
PF00971. EIAV_GP90. 1 hit.
PF11129. EIAV_Rev. 1 hit.

Sequencei

Sequence statusi: Complete.

P20919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESKEARDQ EMNLKEESKE EKRRNDWWKK DPQGPLESDQ WCRVLRQSLP
60 70 80 90 100
EEKIPSQTCI ARRHLGPGPT QHTPSRRDRW IRGQILQTEV LQERLEWRIR
110 120 130 140 150
GVQQAAKELG EVNRGIWREL YFREDQRGDF SAWGGYQRAQ ERLWGEQSSP
160
RVLRPGDSKR RRKHL
Length:165
Mass (Da):19,810
Last modified:February 1, 1991 - v1
Checksum:i04FDEB0830888F36
GO

Sequence cautioni

The sequence AAA43027 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30K → I (PubMed:2164593).Curated1
Sequence conflicti59C → Y in AAA43027 (PubMed:2164593).Curated1
Sequence conflicti88T → A in AAA43027 (PubMed:2164593).Curated1
Sequence conflicti114R → Q in AAA43027 (PubMed:2164593).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36592 Genomic RNA. Translation: AAB02404.1.
M54797 Genomic RNA. Translation: AAA43027.1. Different initiation.
PIRiB46357. ASLJ22.

Similar proteinsi

Entry informationi

Entry nameiREV_EIAVY
AccessioniPrimary (citable) accession number: P20919
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: August 30, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program