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P20918

- PLMN_MOUSE

UniProt

P20918 - PLMN_MOUSE

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Protein
Plasminogen
Gene
Plg
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells By similarity.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.

Catalytic activityi

Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.

Enzyme regulationi

Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Cannot be activated with streptokinase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei624 – 6241Charge relay system By similarity
Active sitei667 – 6671Charge relay system By similarity
Active sitei762 – 7621Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: Ensembl

GO - Biological processi

  1. blood coagulation Source: HGNC
  2. extracellular matrix disassembly Source: Ensembl
  3. fibrinolysis Source: UniProtKB-KW
  4. labyrinthine layer blood vessel development Source: MGI
  5. mononuclear cell migration Source: MGI
  6. muscle cell cellular homeostasis Source: MGI
  7. myoblast differentiation Source: MGI
  8. negative regulation of angiogenesis Source: MGI
  9. negative regulation of cell-substrate adhesion Source: Ensembl
  10. negative regulation of fibrinolysis Source: Ensembl
  11. positive regulation of fibrinolysis Source: Ensembl
  12. proteolysis involved in cellular protein catabolic process Source: Ensembl
  13. tissue regeneration Source: MGI
  14. tissue remodeling Source: UniProtKB-KW
  15. trophoblast giant cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Blood coagulation, Fibrinolysis, Hemostasis, Tissue remodeling

Enzyme and pathway databases

ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.
REACT_199052. Degradation of the extracellular matrix.
REACT_222614. LDL-mediated lipid transport.

Protein family/group databases

MEROPSiS01.233.

Names & Taxonomyi

Protein namesi
Gene namesi
Name:Plg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:97620. Plg.

Subcellular locationi

Secreted By similarity
Note: Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface By similarity.

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. extracellular space Source: HGNC
  3. extrinsic component of external side of plasma membrane Source: Ensembl
  4. extrinsic component of plasma membrane Source: MGI
  5. intracellular membrane-bounded organelle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919
Add
BLAST
Chaini20 – 812793Plasminogen
PRO_0000028069Add
BLAST
Chaini20 – 581562Plasmin heavy chain A
PRO_0000028070Add
BLAST
Peptidei20 – 9778Activation peptide
PRO_0000028071Add
BLAST
Chaini98 – 581484Plasmin heavy chain A, short form
PRO_0000028072Add
BLAST
Chaini98 – ?436339Angiostatin
PRO_0000028073Add
BLAST
Chaini582 – 812231Plasmin light chain B
PRO_0000028074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 73 By similarity
Disulfide bondi53 ↔ 61 By similarity
Disulfide bondi103 ↔ 181 By similarity
Disulfide bondi124 ↔ 164 By similarity
Disulfide bondi152 ↔ 176 By similarity
Disulfide bondi185 ↔ 262 By similarity
Disulfide bondi188 ↔ 316 By similarity
Disulfide bondi206 ↔ 245 By similarity
Disulfide bondi234 ↔ 257 By similarity
Disulfide bondi275 ↔ 352 By similarity
Disulfide bondi296 ↔ 335 By similarity
Disulfide bondi324 ↔ 347 By similarity
Disulfide bondi377 ↔ 454 By similarity
Disulfide bondi398 ↔ 437 By similarity
Disulfide bondi426 ↔ 449 By similarity
Disulfide bondi481 ↔ 560 By similarity
Disulfide bondi502 ↔ 543 By similarity
Disulfide bondi531 ↔ 555 By similarity
Disulfide bondi568 ↔ 687Interchain (between A and B chains) By similarity
Disulfide bondi578 ↔ 586Interchain (between A and B chains) By similarity
Modified residuei598 – 5981Phosphoserine By similarity
Disulfide bondi609 ↔ 625 By similarity
Disulfide bondi701 ↔ 768 By similarity
Disulfide bondi731 ↔ 747 By similarity
Disulfide bondi758 ↔ 786 By similarity

Post-translational modificationi

In the presence of the inhibitor, the activation involves only cleavage after Arg-581, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

MaxQBiP20918.
PaxDbiP20918.
PRIDEiP20918.

2D gel databases

REPRODUCTION-2DPAGEP20918.

PTM databases

PhosphoSiteiP20918.

Miscellaneous databases

PMAP-CutDBP20918.

Expressioni

Gene expression databases

BgeeiP20918.
CleanExiMM_PLG.
GenevestigatoriP20918.

Interactioni

Subunit structurei

Interacts (both mature PLG and the angiostatin peptide) with AMOT and CSPG4. Interacts (via the Kringle domains) with HRG; the interaction tethers PLG to the cell surface and enhances its activation. Interacts (the angiostatin peptide) with ATP5A1; the interaction inhibits most of the angiogenic effects of angiostatin By similarity.

Protein-protein interaction databases

IntActiP20918. 4 interactions.
MINTiMINT-4107912.

Structurei

3D structure databases

ProteinModelPortaliP20918.
SMRiP20918. Positions 20-812.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 9879PAN
Add
BLAST
Domaini103 – 18179Kringle 1
Add
BLAST
Domaini184 – 26279Kringle 2
Add
BLAST
Domaini275 – 35278Kringle 3
Add
BLAST
Domaini377 – 45478Kringle 4
Add
BLAST
Domaini481 – 56080Kringle 5
Add
BLAST
Domaini582 – 810229Peptidase S1
Add
BLAST

Domaini

Kringle domains mediate interaction with CSPG4 By similarity.

Sequence similaritiesi

Contains 5 kringle domains.
Contains 1 PAN domain.

Keywords - Domaini

Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00740000115022.
HOGENOMiHOG000112892.
HOVERGENiHBG004381.
InParanoidiP20918.
KOiK01315.
OMAiEGLEENY.
OrthoDBiEOG75B84T.
TreeFamiTF329901.

Family and domain databases

Gene3Di2.40.20.10. 5 hits.
InterProiIPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR003014. PAN-1_domain.
IPR003609. Pan_app.
IPR023317. Pept_S1A_plasmin.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00051. Kringle. 5 hits.
PF00024. PAN_1. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001150. Plasmin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 5 hits.
SM00473. PAN_AP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 5 hits.
PROSITEiPS00021. KRINGLE_1. 5 hits.
PS50070. KRINGLE_2. 5 hits.
PS50948. PAN. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20918-1 [UniParc]FASTAAdd to Basket

« Hide

MDHKEVILLF LLLLKPGQGD SLDGYISTQG ASLFSLTKKQ LAAGGVSDCL    50
AKCEGETDFV CRSFQYHSKE QQCVIMAENS KTSSIIRMRD VILFEKRVYL 100
SECKTGIGNG YRGTMSRTKS GVACQKWGAT FPHVPNYSPS THPNEGLEEN 150
YCRNPDNDEQ GPWCYTTDPD KRYDYCNIPE CEEECMYCSG EKYEGKISKT 200
MSGLDCQAWD SQSPHAHGYI PAKFPSKNLK MNYCRNPDGE PRPWCFTTDP 250
TKRWEYCDIP RCTTPPPPPS PTYQCLKGRG ENYRGTVSVT VSGKTCQRWS 300
EQTPHRHNRT PENFPCKNLE ENYCRNPDGE TAPWCYTTDS QLRWEYCEIP 350
SCESSASPDQ SDSSVPPEEQ TPVVQECYQS DGQSYRGTSS TTITGKKCQS 400
WAAMFPHRHS KTPENFPDAG LEMNYCRNPD GDKGPWCYTT DPSVRWEYCN 450
LKRCSETGGS VVELPTVSQE PSGPSDSETD CMYGNGKDYR GKTAVTAAGT 500
PCQGWAAQEP HRHSIFTPQT NPRAGLEKNY CRNPDGDVNG PWCYTTNPRK 550
LYDYCDIPLC ASASSFECGK PQVEPKKCPG RVVGGCVANP HSWPWQISLR 600
TRFTGQHFCG GTLIAPEWVL TAAHCLEKSS RPEFYKVILG AHEEYIRGLD 650
VQEISVAKLI LEPNNRDIAL LKLSRPATIT DKVIPACLPS PNYMVADRTI 700
CYITGWGETQ GTFGAGRLKE AQLPVIENKV CNRVEYLNNR VKSTELCAGQ 750
LAGGVDSCQG DSGGPLVCFE KDKYILQGVT SWGLGCARPN KPGVYVRVSR 800
FVDWIEREMR NN 812
Length:812
Mass (Da):90,808
Last modified:June 28, 2011 - v3
Checksum:iE70E1AC8E52844E9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti235 – 2351R → H in AAA50168. 1 Publication
Sequence conflicti525 – 5251G → D in AAA50168. 1 Publication
Sequence conflicti649 – 6491L → S in AAM22156. 1 Publication
Sequence conflicti649 – 6491L → S in AAH14773. 1 Publication
Sequence conflicti649 – 6491L → S in AAH57186. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04766 mRNA. Translation: AAA50168.1.
AF481053 Genomic DNA. Translation: AAM22156.1.
AC087901 Genomic DNA. No translation available.
BC014773 mRNA. Translation: AAH14773.1.
BC057186 mRNA. Translation: AAH57186.1.
AY134430 Genomic DNA. Translation: AAN15805.1.
CCDSiCCDS28390.1.
PIRiA38514. PLMS.
RefSeqiNP_032903.3. NM_008877.3.
UniGeneiMm.971.

Genome annotation databases

EnsembliENSMUST00000014578; ENSMUSP00000014578; ENSMUSG00000059481.
GeneIDi18815.
KEGGimmu:18815.
UCSCiuc008akt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04766 mRNA. Translation: AAA50168.1 .
AF481053 Genomic DNA. Translation: AAM22156.1 .
AC087901 Genomic DNA. No translation available.
BC014773 mRNA. Translation: AAH14773.1 .
BC057186 mRNA. Translation: AAH57186.1 .
AY134430 Genomic DNA. Translation: AAN15805.1 .
CCDSi CCDS28390.1.
PIRi A38514. PLMS.
RefSeqi NP_032903.3. NM_008877.3.
UniGenei Mm.971.

3D structure databases

ProteinModelPortali P20918.
SMRi P20918. Positions 20-812.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P20918. 4 interactions.
MINTi MINT-4107912.

Chemistry

ChEMBLi CHEMBL1075299.

Protein family/group databases

MEROPSi S01.233.

PTM databases

PhosphoSitei P20918.

2D gel databases

REPRODUCTION-2DPAGE P20918.

Proteomic databases

MaxQBi P20918.
PaxDbi P20918.
PRIDEi P20918.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000014578 ; ENSMUSP00000014578 ; ENSMUSG00000059481 .
GeneIDi 18815.
KEGGi mmu:18815.
UCSCi uc008akt.2. mouse.

Organism-specific databases

CTDi 5340.
MGIi MGI:97620. Plg.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00740000115022.
HOGENOMi HOG000112892.
HOVERGENi HBG004381.
InParanoidi P20918.
KOi K01315.
OMAi EGLEENY.
OrthoDBi EOG75B84T.
TreeFami TF329901.

Enzyme and pathway databases

Reactomei REACT_199000. Activation of Matrix Metalloproteinases.
REACT_199052. Degradation of the extracellular matrix.
REACT_222614. LDL-mediated lipid transport.

Miscellaneous databases

ChiTaRSi PLG. mouse.
NextBioi 295166.
PMAP-CutDB P20918.
PROi P20918.
SOURCEi Search...

Gene expression databases

Bgeei P20918.
CleanExi MM_PLG.
Genevestigatori P20918.

Family and domain databases

Gene3Di 2.40.20.10. 5 hits.
InterProi IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR003014. PAN-1_domain.
IPR003609. Pan_app.
IPR023317. Pept_S1A_plasmin.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00051. Kringle. 5 hits.
PF00024. PAN_1. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001150. Plasmin. 1 hit.
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00130. KR. 5 hits.
SM00473. PAN_AP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 5 hits.
PROSITEi PS00021. KRINGLE_1. 5 hits.
PS50070. KRINGLE_2. 5 hits.
PS50948. PAN. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the cDNA coding for mouse plasminogen and localization of the gene to mouse chromosome 17."
    Degen S.J., Bell S.M., Schaefer L.A., Elliott R.W.
    Genomics 8:49-61(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic sequence analysis in the mouse T-complex region."
    Brathwaite M.E., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129/Sv.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "Localization of regulatory elements mediating constitutive and cytokine-stimulated plasminogen gene expression."
    Bannach F.G., Gutierrez A., Fowler B.J., Bugge T.H., Degen J.L., Parmer R.J., Miles L.A.
    J. Biol. Chem. 277:38579-38588(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
    Strain: 129/SvJ.
    Tissue: Liver.
  6. "Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma."
    O'Reilly M.S., Holmgren L., Shing Y., Chen C., Rosenthal R.A., Moses M., Lane W.S., Cao Y., Sage E.H., Folkman J.
    Cell 79:315-328(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF ANGIOSTATIN, PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiPLMN_MOUSE
AccessioniPrimary (citable) accession number: P20918
Secondary accession number(s): Q8CIS2, Q91WJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 28, 2011
Last modified: September 3, 2014
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot.
In the presence of the inhibitor, the activation involves only cleavage after Arg-581, resulting in 2 chains held together by 2 disulfide bonds. Without the inhibitor, the activation involves also removal of the activation peptide.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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