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Protein

Myelin-associated glycoprotein

Gene

Mag

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. Isoform L-MAG is critical for the formation of myelin in the CNS, whereas isoform S-MAG is sufficient to maintain the integrity of myelin in PNS.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Sialic acidBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-MMU-193634. Axonal growth inhibition (RHOA activation).
R-MMU-210991. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin-associated glycoprotein
Alternative name(s):
Siglec-4a
Gene namesi
Name:Mag
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96912. Mag.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 516ExtracellularSequence analysisAdd BLAST497
Transmembranei517 – 536HelicalSequence analysisAdd BLAST20
Topological domaini537 – 626CytoplasmicSequence analysisAdd BLAST90

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • mesaxon Source: Ensembl
  • myelin sheath Source: UniProtKB
  • myelin sheath adaxonal region Source: Ensembl
  • paranode region of axon Source: BHF-UCL
  • plasma membrane Source: UniProtKB
  • Schmidt-Lanterman incisure Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1250416.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000001485720 – 626Myelin-associated glycoproteinAdd BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 165PROSITE-ProRule annotation
Disulfide bondi42 ↔ 100PROSITE-ProRule annotation
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi159 ↔ 217PROSITE-ProRule annotation
Glycosylationi223N-linked (GlcNAc...)Sequence analysis1
Glycosylationi246N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi261 ↔ 305PROSITE-ProRule annotation
Glycosylationi315N-linked (GlcNAc...)Sequence analysis1
Glycosylationi332N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi347 ↔ 392PROSITE-ProRule annotation
Glycosylationi406N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi421 ↔ 430PROSITE-ProRule annotation
Disulfide bondi432 ↔ 488PROSITE-ProRule annotation
Glycosylationi450N-linked (GlcNAc...)Sequence analysis1
Glycosylationi454N-linked (GlcNAc...)Sequence analysis1
Lipidationi531S-palmitoyl cysteineBy similarity1
Modified residuei545PhosphoserineBy similarity1
Modified residuei547PhosphoserineBy similarity1
Modified residuei549PhosphoserineBy similarity1
Modified residuei590PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP20917.
PaxDbiP20917.
PeptideAtlasiP20917.
PRIDEiP20917.

PTM databases

iPTMnetiP20917.
PhosphoSitePlusiP20917.
SwissPalmiP20917.

Expressioni

Tissue specificityi

Expressed by myelinating glial cells in the central and peripheral nervous system. Detected in oligodendrocyte processes before formation of compact myelin. Restricted to the periaxonal space after myelination. Isoform S-MAG is the predominant isoform in CNS and PNS of the adult.2 Publications

Developmental stagei

In CNS isoform L-MAG is the major form synthesized early in development, and it persists as a significant proportion of the MAG present in the adult. In the PNS isoform L-MAG is expressed at modest levels during development; it is absent in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000036634.
CleanExiMM_MAG.
ExpressionAtlasiP20917. baseline and differential.
GenevisibleiP20917. MM.

Interactioni

Subunit structurei

Binds to RTN4R.

Protein-protein interaction databases

BioGridi201285. 1 interactor.
IntActiP20917. 4 interactors.
MINTiMINT-202735.
STRINGi10090.ENSMUSP00000041464.

Chemistry databases

BindingDBiP20917.

Structurei

3D structure databases

ProteinModelPortaliP20917.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 120Ig-like V-typeAdd BLAST99
Domaini139 – 237Ig-like C2-type 1Add BLAST99
Domaini241 – 325Ig-like C2-type 2Add BLAST85
Domaini327 – 412Ig-like C2-type 3Add BLAST86
Domaini413 – 508Ig-like C2-type 4Add BLAST96

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KDW4. Eukaryota.
ENOG410XQVV. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP20917.
KOiK06771.
PhylomeDBiP20917.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform L-MAG (identifier: P20917-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFLATLPLF WIMISASRGG HWGAWMPSTI SAFEGTCVSI PCRFDFPDEL
60 70 80 90 100
RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC
110 120 130 140 150
TLLLSTLSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV
160 170 180 190 200
AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPTVLGRLR EDEGTWVQVS
210 220 230 240 250
LLHFVPTREA NGHRLGCQAA FPNTTLQFEG YASLDVKYPP VIVEMNSSVE
260 270 280 290 300
AIEGSHVSLL CGADSNPPPL LTWMRDGMVL REAVAKSLYL DLEEVTPGED
310 320 330 340 350
GVYACLAENA YGQDNRTVEL SVMYAPWKPT VNGTVVAVEG ETVSILCSTQ
360 370 380 390 400
SNPDPILTIF KEKQILATVI YESQLQLELP AVTPEDDGEY WCVAENQYGQ
410 420 430 440 450
RATAFNLSVE FAPIILLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN
460 470 480 490 500
VTVNETEREF VYSERSGLLL TSILTIRGQA QAPPRVICTS RNLYGTQSLE
510 520 530 540 550
LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESSSFSG
560 570 580 590 600
GDNPHVLYSP EFRISGAPDK YESEKRLGSE RRLLGLRGES PELDLSYSHS
610 620
DLGKRPTKDS YTLTEELAEY AEIRVK
Length:626
Mass (Da):69,260
Last modified:July 15, 1998 - v2
Checksum:i9C797BD6B52B6057
GO
Isoform S-MAG (identifier: P20917-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     574-582: EKRLGSERR → REVSTRDCH
     583-626: Missing.

Show »
Length:582
Mass (Da):64,268
Checksum:i892DD384D653FCDC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002527574 – 582EKRLGSERR → REVSTRDCH in isoform S-MAG. Curated9
Alternative sequenceiVSP_002528583 – 626Missing in isoform S-MAG. CuratedAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31811 mRNA. Translation: AAA39487.1.
M74793
, M74783, M74784, M74785, M74786, M74787, M74788, M74790, M74791 Genomic DNA. Translation: AAA91743.1.
CCDSiCCDS21115.1. [P20917-2]
PIRiB33785.
RefSeqiNP_034888.1. NM_010758.3. [P20917-2]
XP_011248743.1. XM_011250441.2. [P20917-2]
XP_017177497.1. XM_017322008.1. [P20917-2]
UniGeneiMm.241355.

Genome annotation databases

EnsembliENSMUST00000040548; ENSMUSP00000041464; ENSMUSG00000036634. [P20917-2]
ENSMUST00000187137; ENSMUSP00000139564; ENSMUSG00000036634. [P20917-2]
GeneIDi17136.
KEGGimmu:17136.
UCSCiuc009ghb.1. mouse. [P20917-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-4

Functional Glycomics Gateway - Glycan Binding

Siglec-4a [3 Fc Domains]

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31811 mRNA. Translation: AAA39487.1.
M74793
, M74783, M74784, M74785, M74786, M74787, M74788, M74790, M74791 Genomic DNA. Translation: AAA91743.1.
CCDSiCCDS21115.1. [P20917-2]
PIRiB33785.
RefSeqiNP_034888.1. NM_010758.3. [P20917-2]
XP_011248743.1. XM_011250441.2. [P20917-2]
XP_017177497.1. XM_017322008.1. [P20917-2]
UniGeneiMm.241355.

3D structure databases

ProteinModelPortaliP20917.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201285. 1 interactor.
IntActiP20917. 4 interactors.
MINTiMINT-202735.
STRINGi10090.ENSMUSP00000041464.

Chemistry databases

BindingDBiP20917.
ChEMBLiCHEMBL1250416.

PTM databases

iPTMnetiP20917.
PhosphoSitePlusiP20917.
SwissPalmiP20917.

Proteomic databases

MaxQBiP20917.
PaxDbiP20917.
PeptideAtlasiP20917.
PRIDEiP20917.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040548; ENSMUSP00000041464; ENSMUSG00000036634. [P20917-2]
ENSMUST00000187137; ENSMUSP00000139564; ENSMUSG00000036634. [P20917-2]
GeneIDi17136.
KEGGimmu:17136.
UCSCiuc009ghb.1. mouse. [P20917-2]

Organism-specific databases

CTDi4099.
MGIiMGI:96912. Mag.

Phylogenomic databases

eggNOGiENOG410KDW4. Eukaryota.
ENOG410XQVV. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP20917.
KOiK06771.
PhylomeDBiP20917.
TreeFamiTF332441.

Enzyme and pathway databases

ReactomeiR-MMU-193634. Axonal growth inhibition (RHOA activation).
R-MMU-210991. Basigin interactions.

Miscellaneous databases

PROiP20917.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036634.
CleanExiMM_MAG.
ExpressionAtlasiP20917. baseline and differential.
GenevisibleiP20917. MM.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAG_MOUSE
AccessioniPrimary (citable) accession number: P20917
Secondary accession number(s): P16880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.