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Protein

Myelin-associated glycoprotein

Gene

Mag

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. Isoform L-MAG is critical for the formation of myelin in the CNS, whereas isoform S-MAG is sufficient to maintain the integrity of myelin in PNS.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Sialic acidBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-MMU-193634. Axonal growth inhibition (RHOA activation).
R-MMU-210991. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin-associated glycoprotein
Alternative name(s):
Siglec-4a
Gene namesi
Name:Mag
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96912. Mag.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 516497ExtracellularSequence analysisAdd
BLAST
Transmembranei517 – 53620HelicalSequence analysisAdd
BLAST
Topological domaini537 – 62690CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • mesaxon Source: Ensembl
  • myelin sheath Source: UniProtKB
  • myelin sheath adaxonal region Source: Ensembl
  • paranode region of axon Source: BHF-UCL
  • plasma membrane Source: Ensembl
  • Schmidt-Lanterman incisure Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1250416.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 626607Myelin-associated glycoproteinPRO_0000014857Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi37 ↔ 165PROSITE-ProRule annotation
Disulfide bondi42 ↔ 100PROSITE-ProRule annotation
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Disulfide bondi159 ↔ 217PROSITE-ProRule annotation
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis
Disulfide bondi261 ↔ 305PROSITE-ProRule annotation
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence analysis
Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence analysis
Disulfide bondi347 ↔ 392PROSITE-ProRule annotation
Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence analysis
Disulfide bondi421 ↔ 430PROSITE-ProRule annotation
Disulfide bondi432 ↔ 488PROSITE-ProRule annotation
Glycosylationi450 – 4501N-linked (GlcNAc...)Sequence analysis
Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence analysis
Lipidationi531 – 5311S-palmitoyl cysteineBy similarity
Modified residuei545 – 5451PhosphoserineBy similarity
Modified residuei547 – 5471PhosphoserineBy similarity
Modified residuei549 – 5491PhosphoserineBy similarity
Modified residuei590 – 5901PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP20917.
PaxDbiP20917.
PeptideAtlasiP20917.
PRIDEiP20917.

PTM databases

iPTMnetiP20917.
PhosphoSiteiP20917.
SwissPalmiP20917.

Expressioni

Tissue specificityi

Expressed by myelinating glial cells in the central and peripheral nervous system. Detected in oligodendrocyte processes before formation of compact myelin. Restricted to the periaxonal space after myelination. Isoform S-MAG is the predominant isoform in CNS and PNS of the adult.2 Publications

Developmental stagei

In CNS isoform L-MAG is the major form synthesized early in development, and it persists as a significant proportion of the MAG present in the adult. In the PNS isoform L-MAG is expressed at modest levels during development; it is absent in the adult.1 Publication

Gene expression databases

BgeeiP20917.
CleanExiMM_MAG.
ExpressionAtlasiP20917. baseline and differential.
GenevisibleiP20917. MM.

Interactioni

Subunit structurei

Binds to RTN4R.

Protein-protein interaction databases

BioGridi201285. 1 interaction.
IntActiP20917. 4 interactions.
MINTiMINT-202735.
STRINGi10090.ENSMUSP00000041464.

Chemistry

BindingDBiP20917.

Structurei

3D structure databases

ProteinModelPortaliP20917.
SMRiP20917. Positions 22-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 12099Ig-like V-typeAdd
BLAST
Domaini139 – 23799Ig-like C2-type 1Add
BLAST
Domaini241 – 32585Ig-like C2-type 2Add
BLAST
Domaini327 – 41286Ig-like C2-type 3Add
BLAST
Domaini413 – 50896Ig-like C2-type 4Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KDW4. Eukaryota.
ENOG410XQVV. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP20917.
KOiK06771.
OrthoDBiEOG7JDQX2.
PhylomeDBiP20917.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform L-MAG (identifier: P20917-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFLATLPLF WIMISASRGG HWGAWMPSTI SAFEGTCVSI PCRFDFPDEL
60 70 80 90 100
RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC
110 120 130 140 150
TLLLSTLSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV
160 170 180 190 200
AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPTVLGRLR EDEGTWVQVS
210 220 230 240 250
LLHFVPTREA NGHRLGCQAA FPNTTLQFEG YASLDVKYPP VIVEMNSSVE
260 270 280 290 300
AIEGSHVSLL CGADSNPPPL LTWMRDGMVL REAVAKSLYL DLEEVTPGED
310 320 330 340 350
GVYACLAENA YGQDNRTVEL SVMYAPWKPT VNGTVVAVEG ETVSILCSTQ
360 370 380 390 400
SNPDPILTIF KEKQILATVI YESQLQLELP AVTPEDDGEY WCVAENQYGQ
410 420 430 440 450
RATAFNLSVE FAPIILLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN
460 470 480 490 500
VTVNETEREF VYSERSGLLL TSILTIRGQA QAPPRVICTS RNLYGTQSLE
510 520 530 540 550
LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESSSFSG
560 570 580 590 600
GDNPHVLYSP EFRISGAPDK YESEKRLGSE RRLLGLRGES PELDLSYSHS
610 620
DLGKRPTKDS YTLTEELAEY AEIRVK
Length:626
Mass (Da):69,260
Last modified:July 15, 1998 - v2
Checksum:i9C797BD6B52B6057
GO
Isoform S-MAG (identifier: P20917-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     574-582: EKRLGSERR → REVSTRDCH
     583-626: Missing.

Show »
Length:582
Mass (Da):64,268
Checksum:i892DD384D653FCDC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei574 – 5829EKRLGSERR → REVSTRDCH in isoform S-MAG. CuratedVSP_002527
Alternative sequencei583 – 62644Missing in isoform S-MAG. CuratedVSP_002528Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31811 mRNA. Translation: AAA39487.1.
M74793
, M74783, M74784, M74785, M74786, M74787, M74788, M74790, M74791 Genomic DNA. Translation: AAA91743.1.
CCDSiCCDS21115.1. [P20917-2]
PIRiB33785.
RefSeqiNP_034888.1. NM_010758.2. [P20917-2]
XP_011248743.1. XM_011250441.1. [P20917-2]
UniGeneiMm.241355.

Genome annotation databases

EnsembliENSMUST00000040548; ENSMUSP00000041464; ENSMUSG00000036634. [P20917-2]
ENSMUST00000187137; ENSMUSP00000139564; ENSMUSG00000036634. [P20917-2]
GeneIDi17136.
KEGGimmu:17136.
UCSCiuc009ghb.1. mouse. [P20917-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-4

Functional Glycomics Gateway - Glycan Binding

Siglec-4a [3 Fc Domains]

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31811 mRNA. Translation: AAA39487.1.
M74793
, M74783, M74784, M74785, M74786, M74787, M74788, M74790, M74791 Genomic DNA. Translation: AAA91743.1.
CCDSiCCDS21115.1. [P20917-2]
PIRiB33785.
RefSeqiNP_034888.1. NM_010758.2. [P20917-2]
XP_011248743.1. XM_011250441.1. [P20917-2]
UniGeneiMm.241355.

3D structure databases

ProteinModelPortaliP20917.
SMRiP20917. Positions 22-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201285. 1 interaction.
IntActiP20917. 4 interactions.
MINTiMINT-202735.
STRINGi10090.ENSMUSP00000041464.

Chemistry

BindingDBiP20917.
ChEMBLiCHEMBL1250416.

PTM databases

iPTMnetiP20917.
PhosphoSiteiP20917.
SwissPalmiP20917.

Proteomic databases

MaxQBiP20917.
PaxDbiP20917.
PeptideAtlasiP20917.
PRIDEiP20917.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040548; ENSMUSP00000041464; ENSMUSG00000036634. [P20917-2]
ENSMUST00000187137; ENSMUSP00000139564; ENSMUSG00000036634. [P20917-2]
GeneIDi17136.
KEGGimmu:17136.
UCSCiuc009ghb.1. mouse. [P20917-2]

Organism-specific databases

CTDi4099.
MGIiMGI:96912. Mag.

Phylogenomic databases

eggNOGiENOG410KDW4. Eukaryota.
ENOG410XQVV. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP20917.
KOiK06771.
OrthoDBiEOG7JDQX2.
PhylomeDBiP20917.
TreeFamiTF332441.

Enzyme and pathway databases

ReactomeiR-MMU-193634. Axonal growth inhibition (RHOA activation).
R-MMU-210991. Basigin interactions.

Miscellaneous databases

PROiP20917.
SOURCEiSearch...

Gene expression databases

BgeeiP20917.
CleanExiMM_MAG.
ExpressionAtlasiP20917. baseline and differential.
GenevisibleiP20917. MM.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of mouse myelin-associated glycoprotein: a novel alternative splicing pattern."
    Fujita N., Sato S., Kurihara T., Kuwano R., Sakimura K., Inuzuka T., Takahashi Y., Miyatake T.
    Biochem. Biophys. Res. Commun. 165:1162-1169(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS L-MAG AND S-MAG).
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM S-MAG).
    Tissue: Brain.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 78-88 AND 466-477, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Differential expression of MAG isoforms during development."
    Pedraza L., Frey A.B., Hempstead B.L., Colman D.R., Salzer J.L.
    J. Neurosci. Res. 29:141-148(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "Sialoadhesin, myelin-associated glycoprotein and CD22 define a new family of sialic acid-dependent adhesion molecules of the immunoglobulin superfamily."
    Kelm S., Pelz A., Schauer R., Filbin M.T., Tang S., de Bellard M.E., Schnaar R.L., Mahoney J.A., Hartnell A., Bradfield P., Crocker P.R.
    Curr. Biol. 4:965-972(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIALIC ACID-BINDING.
  6. "Multiple functions of the myelin-associated glycoprotein MAG (siglec-4a) in formation and maintenance of myelin."
    Schachner M., Bartsch U.
    Glia 29:154-165(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  7. "Myelin-associated glycoprotein as a functional ligand for the Nogo-66 receptor."
    Liu B.P., Fournier A., GrandPre T., Strittmatter S.M.
    Science 297:1190-1193(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RTN4R.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-590, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiMAG_MOUSE
AccessioniPrimary (citable) accession number: P20917
Secondary accession number(s): P16880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 15, 1998
Last modified: July 6, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.