P20916 (MAG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myelin-associated glycoprotein

Alternative name(s):
Siglec-4a

Gene names

Name:	MAG
Synonyms:	GMA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid By similarity.
Subunit structure	Binds to RTN4R By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 4 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW leukocyte migration Traceable author statement. Source: Reactome negative regulation of axonogenesis Traceable author statement. Source: Reactome nerve growth factor receptor signaling pathway Traceable author statement. Source: Reactome
Cellular_component	Schmidt-Lanterman incisure Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW paranode region of axon Inferred from electronic annotation. Source: Compara plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P20916-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P20916-2)

The sequence of this isoform differs from the canonical sequence as follows:
     574-626: ERRLGSERRLLGLRGEPPELDLSYSHSDLGKRPTKDSYTLTEELAEYAEIRVK → KEVSTLESH

Note: No experimental confirmation available.

Isoform 3 (identifier: P20916-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19
	Chain	20 – 626	607	Myelin-associated glycoprotein		PRO_0000014856
Regions
	Topological domain	20 – 516	497	Extracellular Potential
	Transmembrane	517 – 536	20	Helical; Potential
	Topological domain	537 – 626	90	Cytoplasmic Potential
	Domain	22 – 120	99	Ig-like V-type
	Domain	139 – 237	99	Ig-like C2-type 1
	Domain	241 – 325	85	Ig-like C2-type 2
	Domain	327 – 412	86	Ig-like C2-type 3
	Domain	413 – 508	96	Ig-like C2-type 4
Sites
	Binding site	118	1	Sialic acid By similarity
	Site	332	1	Not glycosylated
Amino acid modifications
	Lipidation	531	1	S-palmitoyl cysteine By similarity
	Glycosylation	99	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	106	1	N-linked (GlcNAc...); partial Ref.6
	Glycosylation	223	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	246	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	315	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	406	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	450	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	454	1	N-linked (GlcNAc...) Ref.6
	Disulfide bond	37 ↔ 165		By similarity
	Disulfide bond	42 ↔ 100		By similarity
	Disulfide bond	159 ↔ 217		By similarity
	Disulfide bond	261 ↔ 305		By similarity
	Disulfide bond	347 ↔ 392		By similarity
	Disulfide bond	421 ↔ 430		By similarity
	Disulfide bond	432 ↔ 488		By similarity
Natural variations
	Alternative sequence	1 – 25	25	Missing in isoform 3.		VSP_045843
	Alternative sequence	574 – 626	53	ERRLG…EIRVK → KEVSTLESH in isoform 2.		VSP_042688
	Natural variant	202	1	L → M. Ref.3 Corresponds to variant rs11084810 [ dbSNP | Ensembl ].		VAR_059399
Experimental info
	Sequence conflict	614	1	T → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: ED2D36B24F21CAAA	FASTA	626	69,069
	10 20 30 40 50 60 MIFLTALPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL RPAVVHGVWY 70 80 90 100 110 120 FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC TLLLSNVSPE LGGKYYFRGD 130 140 150 160 170 180 LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV AGTEVEVSCM VPDNCPELRP ELSWLGHEGL 190 200 210 220 230 240 GEPAVLGRLR EDEGTWVQVS LLHFVPTREA NGHRLGCQAS FPNTTLQFEG YASMDVKYPP 250 260 270 280 290 300 VIVEMNSSVE AIEGSHVSLL CGADSNPPPL LTWMRDGTVL REAVAESLLL ELEEVTPAED 310 320 330 340 350 360 GVYACLAENA YGQDNRTVGL SVMYAPWKPT VNGTMVAVEG ETVSILCSTQ SNPDPILTIF 370 380 390 400 410 420 KEKQILSTVI YESELQLELP AVSPEDDGEY WCVAENQYGQ RATAFNLSVE FAPVLLLESH 430 440 450 460 470 480 CAAARDTVQC LCVVKSNPEP SVAFELPSRN VTVNESEREF VYSERSGLVL TSILTLRGQA 490 500 510 520 530 540 QAPPRVICTA RNLYGAKSLE LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK 550 560 570 580 590 600 NVTESPSFSA GDNPPVLFSS DFRISGAPEK YESERRLGSE RRLLGLRGEP PELDLSYSHS 610 620 DLGKRPTKDS YTLTEELAEY AEIRVK
	Isoform 2 [UniParc]. Checksum: A063EDC5681F3638	FASTA	582	63,966
	10 20 30 40 50 60 MIFLTALPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL RPAVVHGVWY 70 80 90 100 110 120 FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC TLLLSNVSPE LGGKYYFRGD 130 140 150 160 170 180 LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV AGTEVEVSCM VPDNCPELRP ELSWLGHEGL 190 200 210 220 230 240 GEPAVLGRLR EDEGTWVQVS LLHFVPTREA NGHRLGCQAS FPNTTLQFEG YASMDVKYPP 250 260 270 280 290 300 VIVEMNSSVE AIEGSHVSLL CGADSNPPPL LTWMRDGTVL REAVAESLLL ELEEVTPAED 310 320 330 340 350 360 GVYACLAENA YGQDNRTVGL SVMYAPWKPT VNGTMVAVEG ETVSILCSTQ SNPDPILTIF 370 380 390 400 410 420 KEKQILSTVI YESELQLELP AVSPEDDGEY WCVAENQYGQ RATAFNLSVE FAPVLLLESH 430 440 450 460 470 480 CAAARDTVQC LCVVKSNPEP SVAFELPSRN VTVNESEREF VYSERSGLVL TSILTLRGQA 490 500 510 520 530 540 QAPPRVICTA RNLYGAKSLE LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK 550 560 570 580 NVTESPSFSA GDNPPVLFSS DFRISGAPEK YESKEVSTLE SH
	Isoform 3 [UniParc]. Checksum: E51F6F12D9B87B35	FASTA	601	66,224
	10 20 30 40 50 60 MPSSISAFEG TCVSIPCRFD FPDELRPAVV HGVWYFNSPY PKNYPPVVFK SRTQVVHESF 70 80 90 100 110 120 QGRSRLLGDL GLRNCTLLLS NVSPELGGKY YFRGDLGGYN QYTFSEHSVL DIVNTPNIVV 130 140 150 160 170 180 PPEVVAGTEV EVSCMVPDNC PELRPELSWL GHEGLGEPAV LGRLREDEGT WVQVSLLHFV 190 200 210 220 230 240 PTREANGHRL GCQASFPNTT LQFEGYASMD VKYPPVIVEM NSSVEAIEGS HVSLLCGADS 250 260 270 280 290 300 NPPPLLTWMR DGTVLREAVA ESLLLELEEV TPAEDGVYAC LAENAYGQDN RTVGLSVMYA 310 320 330 340 350 360 PWKPTVNGTM VAVEGETVSI LCSTQSNPDP ILTIFKEKQI LSTVIYESEL QLELPAVSPE 370 380 390 400 410 420 DDGEYWCVAE NQYGQRATAF NLSVEFAPVL LLESHCAAAR DTVQCLCVVK SNPEPSVAFE 430 440 450 460 470 480 LPSRNVTVNE SEREFVYSER SGLVLTSILT LRGQAQAPPR VICTARNLYG AKSLELPFQG 490 500 510 520 530 540 AHRLMWAKIG PVGAVVAFAI LIAIVCYITQ TRRKKNVTES PSFSAGDNPP VLFSSDFRIS 550 560 570 580 590 600 GAPEKYESER RLGSERRLLG LRGEPPELDL SYSHSDLGKR PTKDSYTLTE ELAEYAEIRV K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and amino acid sequence for human myelin-associated glycoprotein." Sato S., Fujita N., Kurihara T., Kuwano R., Sakimura K., Takahashi Y., Miyatake T. Biochem. Biophys. Res. Commun. 163:1473-1480(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Molecular cloning of human myelin-associated glycoprotein." Spagnol G., Williams M., Srinivasan J., Golier J., Bauer D., Lebo R.V., Latov N. J. Neurosci. Res. 24:137-142(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT MET-202. Tissue: Brain.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[6]	"Identification of the glycosylated sequons of human myelin-associated glycoprotein." Burger D., Pidoux L., Steck A.J. Biochem. Biophys. Res. Commun. 197:457-464(1993) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-99; ASN-106; ASN-223; ASN-246; ASN-315; ASN-406; ASN-450 AND ASN-454, LACK OF GLYCOSYLATION AT ASN-332.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Siglec-4

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29273 mRNA. Translation: AAA59545.1. AK294644 mRNA. Translation: BAH11831.1. AC002132 Genomic DNA. Translation: AAB58805.1. AD000684 Genomic DNA. No translation available. BC053347 mRNA. Translation: AAH53347.1. BC093045 mRNA. Translation: AAH93045.1.
IPI	IPI00026237. IPI00375253. IPI00979171.
PIR	A61084.
RefSeq	NP_001186145.1. NM_001199216.1. NP_002352.1. NM_002361.3. NP_542167.1. NM_080600.2.
UniGene	Hs.643440.
3D structure databases
ProteinModelPortal	P20916.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-58523N.
IntAct	P20916. 1 interaction.
STRING	9606.ENSP00000262624.
PTM databases
PhosphoSite	P20916.
Polymorphism databases
DMDM	126689.
Proteomic databases
PaxDb	P20916.
PRIDE	P20916.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361922; ENSP00000355234; ENSG00000105695. ENST00000392213; ENSP00000376048; ENSG00000105695. ENST00000537831; ENSP00000440695; ENSG00000105695.
GeneID	4099.
KEGG	hsa:4099.
UCSC	uc002nyy.2. human.
Organism-specific databases
CTD	4099.
GeneCards	GC19P035783.
H-InvDB	HIX0080123.
HGNC	HGNC:6783. MAG.
HPA	CAB009345. HPA012499.
MIM	159460. gene.
neXtProt	NX_P20916.
PharmGKB	PA30541.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG290903.
HOGENOM	HOG000113464.
HOVERGEN	HBG006317.
InParanoid	P20916.
KO	K06771.
OrthoDB	EOG4M3985.
PhylomeDB	P20916.
Enzyme and pathway databases
Pathway_Interaction_DB	p75ntrpathway. p75(NTR)-mediated signaling.
Reactome	REACT_111102. Signal Transduction. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P20916.
Bgee	P20916.
CleanEx	HS_MAG.
Genevestigator	P20916.
GermOnline	ENSG00000105695. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 4 hits.
InterPro	IPR013162. CD80_C2-set. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. [Graphical view]
Pfam	PF08205. C2-set_2. 1 hit. PF07679. I-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00408. IGc2. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 3 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P20916.
ChEMBL	CHEMBL5807.
GenomeRNAi	4099.
NextBio	16074.
PMAP-CutDB	P20916.
SOURCE	Search...

Entry information

Entry name

MAG_HUMAN

Accession

Primary (citable) accession number: P20916
Secondary accession number(s): B7Z2E5, F5GYC0, Q567S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 139 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.