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P20916 (MAG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 151. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myelin-associated glycoprotein
Alternative name(s):
Siglec-4a
Gene names
Name:MAG
Synonyms:GMA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length626 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid By similarity.

Subunit structure

Binds to RTN4R By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P20916-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P20916-2)

The sequence of this isoform differs from the canonical sequence as follows:
     574-626: ERRLGSERRLLGLRGEPPELDLSYSHSDLGKRPTKDSYTLTEELAEYAEIRVK → KEVSTLESH
Note: No experimental confirmation available.
Isoform 3 (identifier: P20916-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919
Chain20 – 626607Myelin-associated glycoprotein
PRO_0000014856

Regions

Topological domain20 – 516497Extracellular Potential
Transmembrane517 – 53620Helical; Potential
Topological domain537 – 62690Cytoplasmic Potential
Domain22 – 12099Ig-like V-type
Domain139 – 23799Ig-like C2-type 1
Domain241 – 32585Ig-like C2-type 2
Domain327 – 41286Ig-like C2-type 3
Domain413 – 50896Ig-like C2-type 4

Sites

Binding site1181Sialic acid By similarity
Site3321Not glycosylated

Amino acid modifications

Lipidation5311S-palmitoyl cysteine By similarity
Glycosylation991N-linked (GlcNAc...) Ref.6
Glycosylation1061N-linked (GlcNAc...); partial Ref.6
Glycosylation2231N-linked (GlcNAc...) Ref.6
Glycosylation2461N-linked (GlcNAc...) Ref.6
Glycosylation3151N-linked (GlcNAc...) Ref.6
Glycosylation4061N-linked (GlcNAc...) Ref.6
Glycosylation4501N-linked (GlcNAc...) Ref.6
Glycosylation4541N-linked (GlcNAc...) Ref.6
Disulfide bond37 ↔ 165 By similarity
Disulfide bond42 ↔ 100 By similarity
Disulfide bond159 ↔ 217 By similarity
Disulfide bond261 ↔ 305 By similarity
Disulfide bond347 ↔ 392 By similarity
Disulfide bond421 ↔ 430 By similarity
Disulfide bond432 ↔ 488 By similarity

Natural variations

Alternative sequence1 – 2525Missing in isoform 3.
VSP_045843
Alternative sequence574 – 62653ERRLG…EIRVK → KEVSTLESH in isoform 2.
VSP_042688
Natural variant2021L → M. Ref.3
Corresponds to variant rs11084810 [ dbSNP | Ensembl ].
VAR_059399

Experimental info

Sequence conflict6141T → S Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: ED2D36B24F21CAAA

FASTA62669,069
        10         20         30         40         50         60 
MIFLTALPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL RPAVVHGVWY 

        70         80         90        100        110        120 
FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC TLLLSNVSPE LGGKYYFRGD 

       130        140        150        160        170        180 
LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV AGTEVEVSCM VPDNCPELRP ELSWLGHEGL 

       190        200        210        220        230        240 
GEPAVLGRLR EDEGTWVQVS LLHFVPTREA NGHRLGCQAS FPNTTLQFEG YASMDVKYPP 

       250        260        270        280        290        300 
VIVEMNSSVE AIEGSHVSLL CGADSNPPPL LTWMRDGTVL REAVAESLLL ELEEVTPAED 

       310        320        330        340        350        360 
GVYACLAENA YGQDNRTVGL SVMYAPWKPT VNGTMVAVEG ETVSILCSTQ SNPDPILTIF 

       370        380        390        400        410        420 
KEKQILSTVI YESELQLELP AVSPEDDGEY WCVAENQYGQ RATAFNLSVE FAPVLLLESH 

       430        440        450        460        470        480 
CAAARDTVQC LCVVKSNPEP SVAFELPSRN VTVNESEREF VYSERSGLVL TSILTLRGQA 

       490        500        510        520        530        540 
QAPPRVICTA RNLYGAKSLE LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK 

       550        560        570        580        590        600 
NVTESPSFSA GDNPPVLFSS DFRISGAPEK YESERRLGSE RRLLGLRGEP PELDLSYSHS 

       610        620 
DLGKRPTKDS YTLTEELAEY AEIRVK 

« Hide

Isoform 2 [UniParc].

Checksum: A063EDC5681F3638
Show »

FASTA58263,966
Isoform 3 [UniParc].

Checksum: E51F6F12D9B87B35
Show »

FASTA60166,224

References

« Hide 'large scale' references
[1]"cDNA cloning and amino acid sequence for human myelin-associated glycoprotein."
Sato S., Fujita N., Kurihara T., Kuwano R., Sakimura K., Takahashi Y., Miyatake T.
Biochem. Biophys. Res. Commun. 163:1473-1480(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Molecular cloning of human myelin-associated glycoprotein."
Spagnol G., Williams M., Srinivasan J., Golier J., Bauer D., Lebo R.V., Latov N.
J. Neurosci. Res. 24:137-142(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT MET-202.
Tissue: Brain.
[4]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[6]"Identification of the glycosylated sequons of human myelin-associated glycoprotein."
Burger D., Pidoux L., Steck A.J.
Biochem. Biophys. Res. Commun. 197:457-464(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-99; ASN-106; ASN-223; ASN-246; ASN-315; ASN-406; ASN-450 AND ASN-454, LACK OF GLYCOSYLATION AT ASN-332.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M29273 mRNA. Translation: AAA59545.1.
AK294644 mRNA. Translation: BAH11831.1.
AC002132 Genomic DNA. Translation: AAB58805.1.
AD000684 Genomic DNA. No translation available.
BC053347 mRNA. Translation: AAH53347.1.
BC093045 mRNA. Translation: AAH93045.1.
CCDSCCDS12455.1. [P20916-1]
CCDS12456.1. [P20916-2]
CCDS56090.1. [P20916-3]
PIRA61084.
RefSeqNP_001186145.1. NM_001199216.1. [P20916-3]
NP_002352.1. NM_002361.3. [P20916-1]
NP_542167.1. NM_080600.2. [P20916-2]
UniGeneHs.643440.

3D structure databases

ProteinModelPortalP20916.
SMRP20916. Positions 26-503.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110273. 11 interactions.
DIPDIP-58523N.
IntActP20916. 1 interaction.
MINTMINT-3009422.
STRING9606.ENSP00000262624.

Chemistry

BindingDBP20916.
ChEMBLCHEMBL5807.

PTM databases

PhosphoSiteP20916.

Polymorphism databases

DMDM126689.

Proteomic databases

PaxDbP20916.
PRIDEP20916.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361922; ENSP00000355234; ENSG00000105695. [P20916-2]
ENST00000392213; ENSP00000376048; ENSG00000105695. [P20916-1]
ENST00000537831; ENSP00000440695; ENSG00000105695. [P20916-3]
GeneID4099.
KEGGhsa:4099.
UCSCuc002nyx.2. human. [P20916-2]
uc002nyy.2. human. [P20916-1]

Organism-specific databases

CTD4099.
GeneCardsGC19P035787.
H-InvDBHIX0080123.
HGNCHGNC:6783. MAG.
HPACAB009345.
HPA012499.
MIM159460. gene.
neXtProtNX_P20916.
PharmGKBPA30541.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG290903.
HOGENOMHOG000113464.
HOVERGENHBG006317.
InParanoidP20916.
KOK06771.
OMACQASFPN.
OrthoDBEOG7JDQX2.
PhylomeDBP20916.
TreeFamTF332441.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpressP20916.
BgeeP20916.
CleanExHS_MAG.
GenevestigatorP20916.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00408. IGc2. 2 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMyelin-associated_glycoprotein.
GenomeRNAi4099.
NextBio16074.
PMAP-CutDBP20916.
PROP20916.
SOURCESearch...

Entry information

Entry nameMAG_HUMAN
AccessionPrimary (citable) accession number: P20916
Secondary accession number(s): B7Z2E5, F5GYC0, Q567S4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM