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Protein

Myelin-associated glycoprotein

Gene

MAG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Sialic acidBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-HSA-193634. Axonal growth inhibition (RHOA activation).
R-HSA-210991. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin-associated glycoprotein
Alternative name(s):
Siglec-4a
Gene namesi
Name:MAG
Synonyms:GMA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:6783. MAG.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 516497ExtracellularSequence analysisAdd
BLAST
Transmembranei517 – 53620HelicalSequence analysisAdd
BLAST
Topological domaini537 – 62690CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Spastic paraplegia 75, autosomal recessive (SPG75)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG75 is characterized by onset in early childhood and is associated with mild to moderate cognitive impairment.
See also OMIM:616680
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti133 – 1331S → R in SPG75; alters proper folding; impairs N-glycosylation; retained in the endoplasmic reticulum; increased proteasome-dependent degradation. 1 Publication
VAR_076224
Natural varianti430 – 4301C → G in SPG75; unknown pathological significance. 1 Publication
VAR_076225

Keywords - Diseasei

Disease mutation, Hereditary spastic paraplegia, Neurodegeneration

Organism-specific databases

MIMi616680. phenotype.
PharmGKBiPA30541.

Chemistry

ChEMBLiCHEMBL5807.

Polymorphism and mutation databases

BioMutaiMAG.
DMDMi126689.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 626607Myelin-associated glycoproteinPRO_0000014856Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi37 ↔ 165PROSITE-ProRule annotation
Disulfide bondi42 ↔ 100PROSITE-ProRule annotation
Glycosylationi99 – 991N-linked (GlcNAc...)1 Publication
Glycosylationi106 – 1061N-linked (GlcNAc...); partial1 Publication
Disulfide bondi159 ↔ 217PROSITE-ProRule annotation
Glycosylationi223 – 2231N-linked (GlcNAc...)1 Publication
Glycosylationi246 – 2461N-linked (GlcNAc...)1 Publication
Disulfide bondi261 ↔ 305PROSITE-ProRule annotation
Glycosylationi315 – 3151N-linked (GlcNAc...)1 Publication
Disulfide bondi347 ↔ 392PROSITE-ProRule annotation
Glycosylationi406 – 4061N-linked (GlcNAc...)1 Publication
Disulfide bondi421 ↔ 430PROSITE-ProRule annotation
Disulfide bondi432 ↔ 488PROSITE-ProRule annotation
Glycosylationi450 – 4501N-linked (GlcNAc...)1 Publication
Glycosylationi454 – 4541N-linked (GlcNAc...)1 Publication
Lipidationi531 – 5311S-palmitoyl cysteineBy similarity
Modified residuei545 – 5451PhosphoserineBy similarity
Modified residuei547 – 5471PhosphoserineBy similarity
Modified residuei549 – 5491PhosphoserineBy similarity

Post-translational modificationi

N-glycosylated.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei332 – 3321Not glycosylated1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP20916.
PeptideAtlasiP20916.
PRIDEiP20916.

PTM databases

iPTMnetiP20916.
PhosphoSiteiP20916.

Miscellaneous databases

PMAP-CutDBP20916.

Expressioni

Gene expression databases

BgeeiENSG00000105695.
CleanExiHS_MAG.
ExpressionAtlasiP20916. baseline and differential.
GenevisibleiP20916. HS.

Organism-specific databases

HPAiCAB009345.
HPA012499.

Interactioni

Subunit structurei

Binds to RTN4R.By similarity

Protein-protein interaction databases

BioGridi110273. 11 interactions.
DIPiDIP-58523N.
IntActiP20916. 1 interaction.
MINTiMINT-3009422.
STRINGi9606.ENSP00000376048.

Chemistry

BindingDBiP20916.

Structurei

3D structure databases

ProteinModelPortaliP20916.
SMRiP20916. Positions 26-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 12099Ig-like V-typeAdd
BLAST
Domaini139 – 23799Ig-like C2-type 1Add
BLAST
Domaini241 – 32585Ig-like C2-type 2Add
BLAST
Domaini327 – 41286Ig-like C2-type 3Add
BLAST
Domaini413 – 50896Ig-like C2-type 4Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IUGJ. Eukaryota.
ENOG410XQVV. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP20916.
KOiK06771.
OMAiCQASFPN.
OrthoDBiEOG091G03Q5.
PhylomeDBiP20916.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20916-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFLTALPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL
60 70 80 90 100
RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC
110 120 130 140 150
TLLLSNVSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV
160 170 180 190 200
AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPAVLGRLR EDEGTWVQVS
210 220 230 240 250
LLHFVPTREA NGHRLGCQAS FPNTTLQFEG YASMDVKYPP VIVEMNSSVE
260 270 280 290 300
AIEGSHVSLL CGADSNPPPL LTWMRDGTVL REAVAESLLL ELEEVTPAED
310 320 330 340 350
GVYACLAENA YGQDNRTVGL SVMYAPWKPT VNGTMVAVEG ETVSILCSTQ
360 370 380 390 400
SNPDPILTIF KEKQILSTVI YESELQLELP AVSPEDDGEY WCVAENQYGQ
410 420 430 440 450
RATAFNLSVE FAPVLLLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN
460 470 480 490 500
VTVNESEREF VYSERSGLVL TSILTLRGQA QAPPRVICTA RNLYGAKSLE
510 520 530 540 550
LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESPSFSA
560 570 580 590 600
GDNPPVLFSS DFRISGAPEK YESERRLGSE RRLLGLRGEP PELDLSYSHS
610 620
DLGKRPTKDS YTLTEELAEY AEIRVK
Length:626
Mass (Da):69,069
Last modified:February 1, 1991 - v1
Checksum:iED2D36B24F21CAAA
GO
Isoform 2 (identifier: P20916-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     574-626: ERRLGSERRLLGLRGEPPELDLSYSHSDLGKRPTKDSYTLTEELAEYAEIRVK → KEVSTLESH

Note: No experimental confirmation available.
Show »
Length:582
Mass (Da):63,966
Checksum:iA063EDC5681F3638
GO
Isoform 3 (identifier: P20916-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.

Note: No experimental confirmation available.
Show »
Length:601
Mass (Da):66,224
Checksum:iE51F6F12D9B87B35
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti614 – 6141T → S (PubMed:2479762).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti133 – 1331S → R in SPG75; alters proper folding; impairs N-glycosylation; retained in the endoplasmic reticulum; increased proteasome-dependent degradation. 1 Publication
VAR_076224
Natural varianti202 – 2021L → M.1 Publication
Corresponds to variant rs11084810 [ dbSNP | Ensembl ].
VAR_059399
Natural varianti430 – 4301C → G in SPG75; unknown pathological significance. 1 Publication
VAR_076225

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2525Missing in isoform 3. 1 PublicationVSP_045843Add
BLAST
Alternative sequencei574 – 62653ERRLG…EIRVK → KEVSTLESH in isoform 2. 1 PublicationVSP_042688Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29273 mRNA. Translation: AAA59545.1.
AK294644 mRNA. Translation: BAH11831.1.
AC002132 Genomic DNA. Translation: AAB58805.1.
AD000684 Genomic DNA. No translation available.
BC053347 mRNA. Translation: AAH53347.1.
BC093045 mRNA. Translation: AAH93045.1.
CCDSiCCDS12455.1. [P20916-1]
CCDS12456.1. [P20916-2]
CCDS56090.1. [P20916-3]
PIRiA61084.
RefSeqiNP_001186145.1. NM_001199216.1. [P20916-3]
NP_002352.1. NM_002361.3. [P20916-1]
NP_542167.1. NM_080600.2. [P20916-2]
UniGeneiHs.643440.

Genome annotation databases

EnsembliENST00000361922; ENSP00000355234; ENSG00000105695. [P20916-2]
ENST00000392213; ENSP00000376048; ENSG00000105695. [P20916-1]
ENST00000537831; ENSP00000440695; ENSG00000105695. [P20916-3]
GeneIDi4099.
KEGGihsa:4099.
UCSCiuc002nyx.3. human. [P20916-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29273 mRNA. Translation: AAA59545.1.
AK294644 mRNA. Translation: BAH11831.1.
AC002132 Genomic DNA. Translation: AAB58805.1.
AD000684 Genomic DNA. No translation available.
BC053347 mRNA. Translation: AAH53347.1.
BC093045 mRNA. Translation: AAH93045.1.
CCDSiCCDS12455.1. [P20916-1]
CCDS12456.1. [P20916-2]
CCDS56090.1. [P20916-3]
PIRiA61084.
RefSeqiNP_001186145.1. NM_001199216.1. [P20916-3]
NP_002352.1. NM_002361.3. [P20916-1]
NP_542167.1. NM_080600.2. [P20916-2]
UniGeneiHs.643440.

3D structure databases

ProteinModelPortaliP20916.
SMRiP20916. Positions 26-503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110273. 11 interactions.
DIPiDIP-58523N.
IntActiP20916. 1 interaction.
MINTiMINT-3009422.
STRINGi9606.ENSP00000376048.

Chemistry

BindingDBiP20916.
ChEMBLiCHEMBL5807.

PTM databases

iPTMnetiP20916.
PhosphoSiteiP20916.

Polymorphism and mutation databases

BioMutaiMAG.
DMDMi126689.

Proteomic databases

PaxDbiP20916.
PeptideAtlasiP20916.
PRIDEiP20916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361922; ENSP00000355234; ENSG00000105695. [P20916-2]
ENST00000392213; ENSP00000376048; ENSG00000105695. [P20916-1]
ENST00000537831; ENSP00000440695; ENSG00000105695. [P20916-3]
GeneIDi4099.
KEGGihsa:4099.
UCSCiuc002nyx.3. human. [P20916-1]

Organism-specific databases

CTDi4099.
GeneCardsiMAG.
H-InvDBHIX0080123.
HGNCiHGNC:6783. MAG.
HPAiCAB009345.
HPA012499.
MIMi159460. gene.
616680. phenotype.
neXtProtiNX_P20916.
PharmGKBiPA30541.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IUGJ. Eukaryota.
ENOG410XQVV. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP20916.
KOiK06771.
OMAiCQASFPN.
OrthoDBiEOG091G03Q5.
PhylomeDBiP20916.
TreeFamiTF332441.

Enzyme and pathway databases

ReactomeiR-HSA-193634. Axonal growth inhibition (RHOA activation).
R-HSA-210991. Basigin interactions.

Miscellaneous databases

ChiTaRSiMAG. human.
GeneWikiiMyelin-associated_glycoprotein.
GenomeRNAii4099.
PMAP-CutDBP20916.
PROiP20916.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105695.
CleanExiHS_MAG.
ExpressionAtlasiP20916. baseline and differential.
GenevisibleiP20916. HS.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAG_HUMAN
AccessioniPrimary (citable) accession number: P20916
Secondary accession number(s): B7Z2E5, F5GYC0, Q567S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 7, 2016
This is version 168 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.