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P20916

- MAG_HUMAN

UniProt

P20916 - MAG_HUMAN

Protein

Myelin-associated glycoprotein

Gene

MAG

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 153 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Sialic acidBy similarity
    Sitei332 – 3321Not glycosylated

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. cell adhesion Source: UniProtKB-KW
    3. leukocyte migration Source: Reactome
    4. negative regulation of axonogenesis Source: Reactome
    5. neurotrophin TRK receptor signaling pathway Source: Reactome
    6. regulation of axonogenesis Source: Reactome
    7. substantia nigra development Source: UniProtKB

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Lectin

    Enzyme and pathway databases

    ReactomeiREACT_12560. Basigin interactions.
    REACT_13815. Axonal growth inhibition (RHOA activation).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myelin-associated glycoprotein
    Alternative name(s):
    Siglec-4a
    Gene namesi
    Name:MAG
    Synonyms:GMA
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:6783. MAG.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. paranode region of axon Source: Ensembl
    3. plasma membrane Source: Reactome
    4. Schmidt-Lanterman incisure Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30541.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Add
    BLAST
    Chaini20 – 626607Myelin-associated glycoproteinPRO_0000014856Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi37 ↔ 165PROSITE-ProRule annotation
    Disulfide bondi42 ↔ 100PROSITE-ProRule annotation
    Glycosylationi99 – 991N-linked (GlcNAc...)1 Publication
    Glycosylationi106 – 1061N-linked (GlcNAc...); partial1 Publication
    Disulfide bondi159 ↔ 217PROSITE-ProRule annotation
    Glycosylationi223 – 2231N-linked (GlcNAc...)1 Publication
    Glycosylationi246 – 2461N-linked (GlcNAc...)1 Publication
    Disulfide bondi261 ↔ 305PROSITE-ProRule annotation
    Glycosylationi315 – 3151N-linked (GlcNAc...)1 Publication
    Disulfide bondi347 ↔ 392PROSITE-ProRule annotation
    Glycosylationi406 – 4061N-linked (GlcNAc...)1 Publication
    Disulfide bondi421 ↔ 430PROSITE-ProRule annotation
    Disulfide bondi432 ↔ 488PROSITE-ProRule annotation
    Glycosylationi450 – 4501N-linked (GlcNAc...)1 Publication
    Glycosylationi454 – 4541N-linked (GlcNAc...)1 Publication
    Lipidationi531 – 5311S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP20916.
    PRIDEiP20916.

    PTM databases

    PhosphoSiteiP20916.

    Miscellaneous databases

    PMAP-CutDBP20916.

    Expressioni

    Gene expression databases

    ArrayExpressiP20916.
    BgeeiP20916.
    CleanExiHS_MAG.
    GenevestigatoriP20916.

    Organism-specific databases

    HPAiCAB009345.
    HPA012499.

    Interactioni

    Subunit structurei

    Binds to RTN4R.By similarity

    Protein-protein interaction databases

    BioGridi110273. 11 interactions.
    DIPiDIP-58523N.
    IntActiP20916. 1 interaction.
    MINTiMINT-3009422.
    STRINGi9606.ENSP00000262624.

    Structurei

    3D structure databases

    ProteinModelPortaliP20916.
    SMRiP20916. Positions 26-503.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 516497ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini537 – 62690CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei517 – 53620HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 12099Ig-like V-typeAdd
    BLAST
    Domaini139 – 23799Ig-like C2-type 1Add
    BLAST
    Domaini241 – 32585Ig-like C2-type 2Add
    BLAST
    Domaini327 – 41286Ig-like C2-type 3Add
    BLAST
    Domaini413 – 50896Ig-like C2-type 4Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG290903.
    HOGENOMiHOG000113464.
    HOVERGENiHBG006317.
    InParanoidiP20916.
    KOiK06771.
    OMAiCQASFPN.
    OrthoDBiEOG7JDQX2.
    PhylomeDBiP20916.
    TreeFamiTF332441.

    Family and domain databases

    Gene3Di2.60.40.10. 4 hits.
    InterProiIPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    [Graphical view]
    PfamiPF08205. C2-set_2. 1 hit.
    PF07679. I-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    SM00408. IGc2. 2 hits.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 3 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P20916-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIFLTALPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL    50
    RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC 100
    TLLLSNVSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV 150
    AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPAVLGRLR EDEGTWVQVS 200
    LLHFVPTREA NGHRLGCQAS FPNTTLQFEG YASMDVKYPP VIVEMNSSVE 250
    AIEGSHVSLL CGADSNPPPL LTWMRDGTVL REAVAESLLL ELEEVTPAED 300
    GVYACLAENA YGQDNRTVGL SVMYAPWKPT VNGTMVAVEG ETVSILCSTQ 350
    SNPDPILTIF KEKQILSTVI YESELQLELP AVSPEDDGEY WCVAENQYGQ 400
    RATAFNLSVE FAPVLLLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN 450
    VTVNESEREF VYSERSGLVL TSILTLRGQA QAPPRVICTA RNLYGAKSLE 500
    LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESPSFSA 550
    GDNPPVLFSS DFRISGAPEK YESERRLGSE RRLLGLRGEP PELDLSYSHS 600
    DLGKRPTKDS YTLTEELAEY AEIRVK 626
    Length:626
    Mass (Da):69,069
    Last modified:February 1, 1991 - v1
    Checksum:iED2D36B24F21CAAA
    GO
    Isoform 2 (identifier: P20916-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         574-626: ERRLGSERRLLGLRGEPPELDLSYSHSDLGKRPTKDSYTLTEELAEYAEIRVK → KEVSTLESH

    Note: No experimental confirmation available.

    Show »
    Length:582
    Mass (Da):63,966
    Checksum:iA063EDC5681F3638
    GO
    Isoform 3 (identifier: P20916-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-25: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:601
    Mass (Da):66,224
    Checksum:iE51F6F12D9B87B35
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti614 – 6141T → S(PubMed:2479762)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti202 – 2021L → M.1 Publication
    Corresponds to variant rs11084810 [ dbSNP | Ensembl ].
    VAR_059399

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2525Missing in isoform 3. 1 PublicationVSP_045843Add
    BLAST
    Alternative sequencei574 – 62653ERRLG…EIRVK → KEVSTLESH in isoform 2. 1 PublicationVSP_042688Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29273 mRNA. Translation: AAA59545.1.
    AK294644 mRNA. Translation: BAH11831.1.
    AC002132 Genomic DNA. Translation: AAB58805.1.
    AD000684 Genomic DNA. No translation available.
    BC053347 mRNA. Translation: AAH53347.1.
    BC093045 mRNA. Translation: AAH93045.1.
    CCDSiCCDS12455.1. [P20916-1]
    CCDS12456.1. [P20916-2]
    CCDS56090.1. [P20916-3]
    PIRiA61084.
    RefSeqiNP_001186145.1. NM_001199216.1. [P20916-3]
    NP_002352.1. NM_002361.3. [P20916-1]
    NP_542167.1. NM_080600.2. [P20916-2]
    UniGeneiHs.643440.

    Genome annotation databases

    EnsembliENST00000361922; ENSP00000355234; ENSG00000105695. [P20916-2]
    ENST00000392213; ENSP00000376048; ENSG00000105695. [P20916-1]
    ENST00000537831; ENSP00000440695; ENSG00000105695. [P20916-3]
    GeneIDi4099.
    KEGGihsa:4099.
    UCSCiuc002nyx.2. human. [P20916-2]
    uc002nyy.2. human. [P20916-1]

    Polymorphism databases

    DMDMi126689.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    Siglec-4

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29273 mRNA. Translation: AAA59545.1 .
    AK294644 mRNA. Translation: BAH11831.1 .
    AC002132 Genomic DNA. Translation: AAB58805.1 .
    AD000684 Genomic DNA. No translation available.
    BC053347 mRNA. Translation: AAH53347.1 .
    BC093045 mRNA. Translation: AAH93045.1 .
    CCDSi CCDS12455.1. [P20916-1 ]
    CCDS12456.1. [P20916-2 ]
    CCDS56090.1. [P20916-3 ]
    PIRi A61084.
    RefSeqi NP_001186145.1. NM_001199216.1. [P20916-3 ]
    NP_002352.1. NM_002361.3. [P20916-1 ]
    NP_542167.1. NM_080600.2. [P20916-2 ]
    UniGenei Hs.643440.

    3D structure databases

    ProteinModelPortali P20916.
    SMRi P20916. Positions 26-503.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110273. 11 interactions.
    DIPi DIP-58523N.
    IntActi P20916. 1 interaction.
    MINTi MINT-3009422.
    STRINGi 9606.ENSP00000262624.

    Chemistry

    BindingDBi P20916.
    ChEMBLi CHEMBL5807.

    PTM databases

    PhosphoSitei P20916.

    Polymorphism databases

    DMDMi 126689.

    Proteomic databases

    PaxDbi P20916.
    PRIDEi P20916.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361922 ; ENSP00000355234 ; ENSG00000105695 . [P20916-2 ]
    ENST00000392213 ; ENSP00000376048 ; ENSG00000105695 . [P20916-1 ]
    ENST00000537831 ; ENSP00000440695 ; ENSG00000105695 . [P20916-3 ]
    GeneIDi 4099.
    KEGGi hsa:4099.
    UCSCi uc002nyx.2. human. [P20916-2 ]
    uc002nyy.2. human. [P20916-1 ]

    Organism-specific databases

    CTDi 4099.
    GeneCardsi GC19P035787.
    H-InvDB HIX0080123.
    HGNCi HGNC:6783. MAG.
    HPAi CAB009345.
    HPA012499.
    MIMi 159460. gene.
    neXtProti NX_P20916.
    PharmGKBi PA30541.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG290903.
    HOGENOMi HOG000113464.
    HOVERGENi HBG006317.
    InParanoidi P20916.
    KOi K06771.
    OMAi CQASFPN.
    OrthoDBi EOG7JDQX2.
    PhylomeDBi P20916.
    TreeFami TF332441.

    Enzyme and pathway databases

    Reactomei REACT_12560. Basigin interactions.
    REACT_13815. Axonal growth inhibition (RHOA activation).

    Miscellaneous databases

    GeneWikii Myelin-associated_glycoprotein.
    GenomeRNAii 4099.
    NextBioi 16074.
    PMAP-CutDB P20916.
    PROi P20916.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P20916.
    Bgeei P20916.
    CleanExi HS_MAG.
    Genevestigatori P20916.

    Family and domain databases

    Gene3Di 2.60.40.10. 4 hits.
    InterProi IPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    [Graphical view ]
    Pfami PF08205. C2-set_2. 1 hit.
    PF07679. I-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    SM00408. IGc2. 2 hits.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and amino acid sequence for human myelin-associated glycoprotein."
      Sato S., Fujita N., Kurihara T., Kuwano R., Sakimura K., Takahashi Y., Miyatake T.
      Biochem. Biophys. Res. Commun. 163:1473-1480(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT MET-202.
      Tissue: Brain.
    4. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain.
    6. "Identification of the glycosylated sequons of human myelin-associated glycoprotein."
      Burger D., Pidoux L., Steck A.J.
      Biochem. Biophys. Res. Commun. 197:457-464(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-99; ASN-106; ASN-223; ASN-246; ASN-315; ASN-406; ASN-450 AND ASN-454, LACK OF GLYCOSYLATION AT ASN-332.

    Entry informationi

    Entry nameiMAG_HUMAN
    AccessioniPrimary (citable) accession number: P20916
    Secondary accession number(s): B7Z2E5, F5GYC0, Q567S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 153 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3