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Protein

Myelin-associated glycoprotein

Gene

MAG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Sialic acidBy similarity
Sitei332 – 3321Not glycosylated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiREACT_12560. Basigin interactions.
REACT_13815. Axonal growth inhibition (RHOA activation).

Names & Taxonomyi

Protein namesi
Recommended name:
Myelin-associated glycoprotein
Alternative name(s):
Siglec-4a
Gene namesi
Name:MAG
Synonyms:GMA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:6783. MAG.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 516497ExtracellularSequence AnalysisAdd
BLAST
Transmembranei517 – 53620HelicalSequence AnalysisAdd
BLAST
Topological domaini537 – 62690CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30541.

Polymorphism and mutation databases

BioMutaiMAG.
DMDMi126689.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 626607Myelin-associated glycoproteinPRO_0000014856Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi37 ↔ 165PROSITE-ProRule annotation
Disulfide bondi42 ↔ 100PROSITE-ProRule annotation
Glycosylationi99 – 991N-linked (GlcNAc...)1 Publication
Glycosylationi106 – 1061N-linked (GlcNAc...); partial1 Publication
Disulfide bondi159 ↔ 217PROSITE-ProRule annotation
Glycosylationi223 – 2231N-linked (GlcNAc...)1 Publication
Glycosylationi246 – 2461N-linked (GlcNAc...)1 Publication
Disulfide bondi261 ↔ 305PROSITE-ProRule annotation
Glycosylationi315 – 3151N-linked (GlcNAc...)1 Publication
Disulfide bondi347 ↔ 392PROSITE-ProRule annotation
Glycosylationi406 – 4061N-linked (GlcNAc...)1 Publication
Disulfide bondi421 ↔ 430PROSITE-ProRule annotation
Disulfide bondi432 ↔ 488PROSITE-ProRule annotation
Glycosylationi450 – 4501N-linked (GlcNAc...)1 Publication
Glycosylationi454 – 4541N-linked (GlcNAc...)1 Publication
Lipidationi531 – 5311S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP20916.
PRIDEiP20916.

PTM databases

PhosphoSiteiP20916.

Miscellaneous databases

PMAP-CutDBP20916.

Expressioni

Gene expression databases

BgeeiP20916.
CleanExiHS_MAG.
ExpressionAtlasiP20916. baseline and differential.
GenevisibleiP20916. HS.

Organism-specific databases

HPAiCAB009345.
HPA012499.

Interactioni

Subunit structurei

Binds to RTN4R.By similarity

Protein-protein interaction databases

BioGridi110273. 11 interactions.
DIPiDIP-58523N.
IntActiP20916. 1 interaction.
MINTiMINT-3009422.
STRINGi9606.ENSP00000376048.

Structurei

3D structure databases

ProteinModelPortaliP20916.
SMRiP20916. Positions 26-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 12099Ig-like V-typeAdd
BLAST
Domaini139 – 23799Ig-like C2-type 1Add
BLAST
Domaini241 – 32585Ig-like C2-type 2Add
BLAST
Domaini327 – 41286Ig-like C2-type 3Add
BLAST
Domaini413 – 50896Ig-like C2-type 4Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290903.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP20916.
KOiK06771.
OMAiCQASFPN.
OrthoDBiEOG7JDQX2.
PhylomeDBiP20916.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 2 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20916-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFLTALPLF WIMISASRGG HWGAWMPSSI SAFEGTCVSI PCRFDFPDEL
60 70 80 90 100
RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC
110 120 130 140 150
TLLLSNVSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV
160 170 180 190 200
AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPAVLGRLR EDEGTWVQVS
210 220 230 240 250
LLHFVPTREA NGHRLGCQAS FPNTTLQFEG YASMDVKYPP VIVEMNSSVE
260 270 280 290 300
AIEGSHVSLL CGADSNPPPL LTWMRDGTVL REAVAESLLL ELEEVTPAED
310 320 330 340 350
GVYACLAENA YGQDNRTVGL SVMYAPWKPT VNGTMVAVEG ETVSILCSTQ
360 370 380 390 400
SNPDPILTIF KEKQILSTVI YESELQLELP AVSPEDDGEY WCVAENQYGQ
410 420 430 440 450
RATAFNLSVE FAPVLLLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN
460 470 480 490 500
VTVNESEREF VYSERSGLVL TSILTLRGQA QAPPRVICTA RNLYGAKSLE
510 520 530 540 550
LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESPSFSA
560 570 580 590 600
GDNPPVLFSS DFRISGAPEK YESERRLGSE RRLLGLRGEP PELDLSYSHS
610 620
DLGKRPTKDS YTLTEELAEY AEIRVK
Length:626
Mass (Da):69,069
Last modified:February 1, 1991 - v1
Checksum:iED2D36B24F21CAAA
GO
Isoform 2 (identifier: P20916-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     574-626: ERRLGSERRLLGLRGEPPELDLSYSHSDLGKRPTKDSYTLTEELAEYAEIRVK → KEVSTLESH

Note: No experimental confirmation available.
Show »
Length:582
Mass (Da):63,966
Checksum:iA063EDC5681F3638
GO
Isoform 3 (identifier: P20916-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.

Note: No experimental confirmation available.
Show »
Length:601
Mass (Da):66,224
Checksum:iE51F6F12D9B87B35
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti614 – 6141T → S (PubMed:2479762).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti202 – 2021L → M.1 Publication
Corresponds to variant rs11084810 [ dbSNP | Ensembl ].
VAR_059399

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2525Missing in isoform 3. 1 PublicationVSP_045843Add
BLAST
Alternative sequencei574 – 62653ERRLG…EIRVK → KEVSTLESH in isoform 2. 1 PublicationVSP_042688Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29273 mRNA. Translation: AAA59545.1.
AK294644 mRNA. Translation: BAH11831.1.
AC002132 Genomic DNA. Translation: AAB58805.1.
AD000684 Genomic DNA. No translation available.
BC053347 mRNA. Translation: AAH53347.1.
BC093045 mRNA. Translation: AAH93045.1.
CCDSiCCDS12455.1. [P20916-1]
CCDS12456.1. [P20916-2]
CCDS56090.1. [P20916-3]
PIRiA61084.
RefSeqiNP_001186145.1. NM_001199216.1. [P20916-3]
NP_002352.1. NM_002361.3. [P20916-1]
NP_542167.1. NM_080600.2. [P20916-2]
UniGeneiHs.643440.

Genome annotation databases

EnsembliENST00000361922; ENSP00000355234; ENSG00000105695. [P20916-2]
ENST00000392213; ENSP00000376048; ENSG00000105695.
ENST00000537831; ENSP00000440695; ENSG00000105695. [P20916-3]
GeneIDi4099.
KEGGihsa:4099.
UCSCiuc002nyx.2. human. [P20916-2]
uc002nyy.2. human. [P20916-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29273 mRNA. Translation: AAA59545.1.
AK294644 mRNA. Translation: BAH11831.1.
AC002132 Genomic DNA. Translation: AAB58805.1.
AD000684 Genomic DNA. No translation available.
BC053347 mRNA. Translation: AAH53347.1.
BC093045 mRNA. Translation: AAH93045.1.
CCDSiCCDS12455.1. [P20916-1]
CCDS12456.1. [P20916-2]
CCDS56090.1. [P20916-3]
PIRiA61084.
RefSeqiNP_001186145.1. NM_001199216.1. [P20916-3]
NP_002352.1. NM_002361.3. [P20916-1]
NP_542167.1. NM_080600.2. [P20916-2]
UniGeneiHs.643440.

3D structure databases

ProteinModelPortaliP20916.
SMRiP20916. Positions 26-503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110273. 11 interactions.
DIPiDIP-58523N.
IntActiP20916. 1 interaction.
MINTiMINT-3009422.
STRINGi9606.ENSP00000376048.

Chemistry

BindingDBiP20916.
ChEMBLiCHEMBL5807.

PTM databases

PhosphoSiteiP20916.

Polymorphism and mutation databases

BioMutaiMAG.
DMDMi126689.

Proteomic databases

PaxDbiP20916.
PRIDEiP20916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361922; ENSP00000355234; ENSG00000105695. [P20916-2]
ENST00000392213; ENSP00000376048; ENSG00000105695.
ENST00000537831; ENSP00000440695; ENSG00000105695. [P20916-3]
GeneIDi4099.
KEGGihsa:4099.
UCSCiuc002nyx.2. human. [P20916-2]
uc002nyy.2. human. [P20916-1]

Organism-specific databases

CTDi4099.
GeneCardsiGC19P035956.
H-InvDBHIX0080123.
HGNCiHGNC:6783. MAG.
HPAiCAB009345.
HPA012499.
MIMi159460. gene.
neXtProtiNX_P20916.
PharmGKBiPA30541.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG290903.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000113464.
HOVERGENiHBG006317.
InParanoidiP20916.
KOiK06771.
OMAiCQASFPN.
OrthoDBiEOG7JDQX2.
PhylomeDBiP20916.
TreeFamiTF332441.

Enzyme and pathway databases

ReactomeiREACT_12560. Basigin interactions.
REACT_13815. Axonal growth inhibition (RHOA activation).

Miscellaneous databases

ChiTaRSiMAG. human.
GeneWikiiMyelin-associated_glycoprotein.
GenomeRNAii4099.
NextBioi16074.
PMAP-CutDBP20916.
PROiP20916.
SOURCEiSearch...

Gene expression databases

BgeeiP20916.
CleanExiHS_MAG.
ExpressionAtlasiP20916. baseline and differential.
GenevisibleiP20916. HS.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 2 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and amino acid sequence for human myelin-associated glycoprotein."
    Sato S., Fujita N., Kurihara T., Kuwano R., Sakimura K., Takahashi Y., Miyatake T.
    Biochem. Biophys. Res. Commun. 163:1473-1480(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT MET-202.
    Tissue: Brain.
  4. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  6. "Identification of the glycosylated sequons of human myelin-associated glycoprotein."
    Burger D., Pidoux L., Steck A.J.
    Biochem. Biophys. Res. Commun. 197:457-464(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-99; ASN-106; ASN-223; ASN-246; ASN-315; ASN-406; ASN-450 AND ASN-454, LACK OF GLYCOSYLATION AT ASN-332.

Entry informationi

Entry nameiMAG_HUMAN
AccessioniPrimary (citable) accession number: P20916
Secondary accession number(s): B7Z2E5, F5GYC0, Q567S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 22, 2015
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.