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P20911

- CDK7_XENLA

UniProt

P20911 - CDK7_XENLA

Protein

Cyclin-dependent kinase 7

Gene

cdk7

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of cdk1/cyclin-B during G2-M transition, and for activation of cdk2/cyclins during G1-S transition (but not complex formation). cdk7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. CAK activates the cyclin-associated kinases cdk1, cdk2, cdk4 and cdk6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (polr2a), allowing its escape from the promoter and elongation of the transcripts By similarity. Involved in negative regulation of the meiotic maturation of Xenopus oocytes.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-176 is required for enzymatic activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei47 – 471ATPPROSITE-ProRule annotation
    Active sitei143 – 1431Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi24 – 329ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 6726.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 7 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    40 kDa protein kinase
    CDC2/CDK2,4-activating kinase
    Cell division protein kinase 7
    P40 MO15
    Gene namesi
    Name:cdk7
    Synonyms:mo15
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-485511. cdk7.

    Subcellular locationi

    Nucleus
    Note: In the prominent nucleus of oocytes.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 352352Cyclin-dependent kinase 7PRO_0000085795Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei170 – 1701Phosphoserine; by CDK1 and CDK21 Publication
    Modified residuei176 – 1761Phosphothreonine; by CDK21 Publication

    Post-translational modificationi

    Phosphorylation of Ser-170 during mitosis inactivates the enzyme. Phosphorylation of Thr-176 is required for activity. Phosphorylated at Ser-170 and Thr-176 by CDK2 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Developmental stagei

    Expressed only in non-mature oocytes.

    Interactioni

    Subunit structurei

    Probably associates with cyclin-H (ccnh) and mat1 to form a multimeric active enzyme.

    Protein-protein interaction databases

    BioGridi100785. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP20911.
    SMRiP20911. Positions 19-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 301284Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG014652.
    KOiK02202.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20911-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGIAARGVD VRSRAKQYEK LDFLGEGQFA TVYKARDKNT DRIVAIKKIK    50
    LGHRAEANDG INRTALREIK LLQELSHPNI IGLLDAFGHK SNISLVFDFM 100
    ETDLEVIIKD TSLVLTPAHI KSYMLMTLQG LEYLHHLWIL HRDLKPNNLL 150
    LDENGVLKLA DFGLAKSFGS PNRIYTHQVV TRWYRSPELL FGARMYGVGV 200
    DMWAVGCILA ELLLRVPFLP GDSDLDQLTR IFETLGTPTE EQWPGMSSLP 250
    DYVAFKSFPG TPLHLIFIAA GDDLLELLQG LFTFNPCARC TASQALRKRY 300
    FSNRPAPTPG NLLPRPNCSI EALKEQQNLN LGIKRKRTEG MDQKDIAKKL 350
    SF 352
    Length:352
    Mass (Da):39,691
    Last modified:February 1, 1991 - v1
    Checksum:iC4E21D21509317C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53962 mRNA. Translation: CAA37915.1.
    BC130134 mRNA. Translation: AAI30135.1.
    PIRiS12091.
    RefSeqiNP_001084361.1. NM_001090892.1.
    UniGeneiXl.15107.

    Genome annotation databases

    GeneIDi399461.
    KEGGixla:399461.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53962 mRNA. Translation: CAA37915.1 .
    BC130134 mRNA. Translation: AAI30135.1 .
    PIRi S12091.
    RefSeqi NP_001084361.1. NM_001090892.1.
    UniGenei Xl.15107.

    3D structure databases

    ProteinModelPortali P20911.
    SMRi P20911. Positions 19-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 100785. 3 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399461.
    KEGGi xla:399461.

    Organism-specific databases

    CTDi 1022.
    Xenbasei XB-GENE-485511. cdk7.

    Phylogenomic databases

    HOVERGENi HBG014652.
    KOi K02202.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 6726.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes."
      Shuttleworth J., Godfrey R., Colman A.
      EMBO J. 9:3233-3240(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Oocyte.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.
    3. "p40MO15 associates with a p36 subunit and requires both nuclear translocation and Thr176 phosphorylation to generate cdk-activating kinase activity in Xenopus oocytes."
      Labbe J.-C., Martinez A.-M., Fesquet D., Capony J.-P., Darbon J.-M., Derancourt J., Devault A., Morin N., Cavadore J.-C., Doree M.
      EMBO J. 13:5155-5164(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-170 AND THR-176, INTERACTION WITH CCNH.
      Tissue: Oocyte.

    Entry informationi

    Entry nameiCDK7_XENLA
    AccessioniPrimary (citable) accession number: P20911
    Secondary accession number(s): A2BDA1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3