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P20911 (CDK7_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 7

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
40 kDa protein kinase
CDC2/CDK2,4-activating kinase
Cell division protein kinase 7
P40 MO15
Gene names
Name:cdk7
Synonyms:mo15
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of cdk1/cyclin-B during G2-M transition, and for activation of cdk2/cyclins during G1-S transition (but not complex formation). cdk7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. CAK activates the cyclin-associated kinases cdk1, cdk2, cdk4 and cdk6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (polr2a), allowing its escape from the promoter and elongation of the transcripts By similarity. Involved in negative regulation of the meiotic maturation of Xenopus oocytes.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulation

Phosphorylation at Thr-176 is required for enzymatic activity.

Subunit structure

Probably associates with cyclin-H (ccnh) and mat1 to form a multimeric active enzyme.

Subcellular location

Nucleus. Note: In the prominent nucleus of oocytes.

Developmental stage

Expressed only in non-mature oocytes.

Post-translational modification

Phosphorylation of Ser-170 during mitosis inactivates the enzyme. Phosphorylation of Thr-176 is required for activity. Phosphorylated at Ser-170 and Thr-176 by CDK2 By similarity. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352Cyclin-dependent kinase 7
PRO_0000085795

Regions

Domain18 – 301284Protein kinase
Nucleotide binding24 – 329ATP By similarity

Sites

Active site1431Proton acceptor By similarity
Binding site471ATP By similarity

Amino acid modifications

Modified residue1701Phosphoserine; by CDK1 and CDK2 Ref.3
Modified residue1761Phosphothreonine; by CDK2 Ref.3

Sequences

Sequence LengthMass (Da)Tools
P20911 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: C4E21D21509317C4

FASTA35239,691
        10         20         30         40         50         60 
MEGIAARGVD VRSRAKQYEK LDFLGEGQFA TVYKARDKNT DRIVAIKKIK LGHRAEANDG 

        70         80         90        100        110        120 
INRTALREIK LLQELSHPNI IGLLDAFGHK SNISLVFDFM ETDLEVIIKD TSLVLTPAHI 

       130        140        150        160        170        180 
KSYMLMTLQG LEYLHHLWIL HRDLKPNNLL LDENGVLKLA DFGLAKSFGS PNRIYTHQVV 

       190        200        210        220        230        240 
TRWYRSPELL FGARMYGVGV DMWAVGCILA ELLLRVPFLP GDSDLDQLTR IFETLGTPTE 

       250        260        270        280        290        300 
EQWPGMSSLP DYVAFKSFPG TPLHLIFIAA GDDLLELLQG LFTFNPCARC TASQALRKRY 

       310        320        330        340        350 
FSNRPAPTPG NLLPRPNCSI EALKEQQNLN LGIKRKRTEG MDQKDIAKKL SF 

« Hide

References

« Hide 'large scale' references
[1]"p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes."
Shuttleworth J., Godfrey R., Colman A.
EMBO J. 9:3233-3240(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Oocyte.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
[3]"p40MO15 associates with a p36 subunit and requires both nuclear translocation and Thr176 phosphorylation to generate cdk-activating kinase activity in Xenopus oocytes."
Labbe J.-C., Martinez A.-M., Fesquet D., Capony J.-P., Darbon J.-M., Derancourt J., Devault A., Morin N., Cavadore J.-C., Doree M.
EMBO J. 13:5155-5164(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-170 AND THR-176, INTERACTION WITH CCNH.
Tissue: Oocyte.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53962 mRNA. Translation: CAA37915.1.
BC130134 mRNA. Translation: AAI30135.1.
PIRS12091.
RefSeqNP_001084361.1. NM_001090892.1.
UniGeneXl.15107.

3D structure databases

ProteinModelPortalP20911.
SMRP20911. Positions 19-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid100785. 3 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399461.
KEGGxla:399461.

Organism-specific databases

CTD1022.
XenbaseXB-GENE-485511. cdk7.

Phylogenomic databases

HOVERGENHBG014652.
KOK02202.

Enzyme and pathway databases

BRENDA2.7.11.22. 6726.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK7_XENLA
AccessionPrimary (citable) accession number: P20911
Secondary accession number(s): A2BDA1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: February 19, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families