P20905 (5HT1R_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 1
Short name=5-HT receptor

Alternative name(s):
5HT-dro
Serotonin receptor 1

Gene names

Name:	5-HT7
Synonyms:	5HT-R1
ORF Names:	CG12073

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed predominantly in adult heads. Ref.1
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of adenylate cyclase activity by serotonin receptor signaling pathway Traceable author statement. Source: FlyBase female mating behavior Inferred from mutant phenotype. Source: FlyBase male courtship behavior, orientation prior to leg tapping and wing vibration Inferred from mutant phenotype. Source: FlyBase male courtship behavior, proboscis-mediated licking Inferred from mutant phenotype. Source: FlyBase male courtship behavior, veined wing vibration Inferred from mutant phenotype. Source: FlyBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dopamine receptor activity Inferred from electronic annotation. Source: InterPro serotonin receptor activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	5-hydroxytryptamine receptor 1		PRO_0000068941
Regions
	Transmembrane	29 – 51	23	Helical; Name=0; Potential
	Transmembrane	165 – 188	24	Helical; Name=1; Potential
	Topological domain	189 – 198	10	Cytoplasmic By similarity
	Transmembrane	199 – 222	24	Helical; Name=2; By similarity
	Topological domain	223 – 236	14	Extracellular By similarity
	Transmembrane	237 – 258	22	Helical; Name=3; By similarity
	Topological domain	259 – 278	20	Cytoplasmic By similarity
	Transmembrane	279 – 302	24	Helical; Name=4; By similarity
	Topological domain	303 – 330	28	Extracellular By similarity
	Transmembrane	331 – 353	23	Helical; Name=5; By similarity
	Topological domain	354 – 454	101	Cytoplasmic By similarity
	Transmembrane	455 – 476	22	Helical; Name=6; By similarity
	Topological domain	477 – 487	11	Extracellular By similarity
	Transmembrane	488 – 510	23	Helical; Name=7; By similarity
	Topological domain	511 – 564	54	Cytoplasmic By similarity
	Repeat	89 – 90	2	1
	Repeat	91 – 92	2	2
	Repeat	93 – 94	2	3
	Repeat	95 – 96	2	4
	Repeat	97 – 98	2	5
	Repeat	99 – 100	2	6
	Repeat	101 – 102	2	7
	Repeat	103 – 104	2	8
	Repeat	105 – 106	2	9
	Region	89 – 106	18	9 X 2 AA tandem repeats of G-S
Amino acid modifications
	Disulfide bond	235 ↔ 314		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20905 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 0C8B9F8DA63D8095	FASTA	564	60,861
	10 20 30 40 50 60 MALSGQDWRR HQSHRQHRNH RTQGNHQKLI STATLTLFVL FLSSWIAYAA GKATVPAPLV 70 80 90 100 110 120 EGETESATSQ DFNSSSAFLG AIASASSTGS GSGSGSGSGS GSGSGSYGLA SMNSSPIAIV 130 140 150 160 170 180 SYQGITSSNL GDSNTTLVPL SDTPLLLEEF AAGEFVLPPL TSIFVSIVLL IVILGTVVGN 190 200 210 220 230 240 VLVCIAVCMV RKLRRPCNYL LVSLALSDLC VALLVMPMAL LYEVLEKWNF GPLLCDIWVS 250 260 270 280 290 300 FDVLCCTASI LNLCAISVDR YLAITKPLEY GVKRTPRRMM LCVGIVWLAA ACISLPPLLI 310 320 330 340 350 360 LGNEHEDEEG QPICTVCQNF AYQIYATLGS FYIPLSVMLF VYYQIFRAAR RIVLEEKRAQ 370 380 390 400 410 420 THLQQALNGT GSPSAPQAPP LGHTELASSG NGQRHSSVGN TSLTYSTCGG LSSGGGALAG 430 440 450 460 470 480 HGSGGGVSGS TGLLGSPHHK KLRFQLAKEK KASTTLGIIM SAFTVCWLPF FILALIRPFE 490 500 510 520 530 540 TMHVPASLSS LFLWLGYANS LLNPIIYATL NRDFRKPFQE ILYFRCSSLN TMMRENYYQD 550 560 QYGEPPSQRV MLGDERHGAR ESFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a Drosophila serotonin receptor that activates adenylate cyclase." Witz P., Amlaiky N., Plassat J.-L., Maroteaux L., Borrelli E., Hen R. Proc. Natl. Acad. Sci. U.S.A. 87:8940-8944(1990) [PubMed: 2174167] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Oregon-R. Tissue: Head.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55533 mRNA. Translation: AAA28305.1. AE014297 Genomic DNA. Translation: AAF57104.1. AY118491 mRNA. Translation: AAM49860.1.
PIR	A38271.
RefSeq	NP_524599.1. NM_079860.2.
UniGene	Dm.1903.
3D structure databases
ProteinModelPortal	P20905.
SMR	P20905. Positions 160-523.
ModBase	Search...
Protein-protein interaction databases
STRING	P20905.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	P20905.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085746; FBpp0085108; FBgn0004573.
GeneID	43669.
KEGG	dme:Dmel_CG12073.
NMPDR	fig|7227.3.peg.15534.
Organism-specific databases
CTD	43669.
FlyBase	FBgn0004573. 5-HT7.
Phylogenomic databases
eggNOG	inNOG05035.
GeneTree	EMGT00050000005441.
InParanoid	P20905.
OMA	AYQIYAT.
OrthoDB	EOG4W9GKS.
PhylomeDB	P20905.
Gene expression databases
ArrayExpress	P20905.
Bgee	P20905.
GermOnline	CG12073. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR000929. Dopamine_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
KO	K04163.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00242. DOPAMINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	835175.

Entry information

Entry name

5HT1R_DROME

Accession

Primary (citable) accession number: P20905
Secondary accession number(s): Q9VA21

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project