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Protein

Citrate synthase

Gene

aarA

Organism
Acetobacter aceti
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei313 – 3131PROSITE-ProRule annotation
Active sitei371 – 3711PROSITE-ProRule annotation

GO - Molecular functioni

  1. citrate (Si)-synthase activity Source: InterPro

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17983.
BRENDAi2.3.3.16. 33.
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Alternative name(s):
Acetic acid resistance protein
Gene namesi
Name:aarA
OrganismiAcetobacter aceti
Taxonomic identifieri435 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacterAcetobacter subgen. Acetobacter

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436Citrate synthasePRO_0000169923Add
BLAST

Proteomic databases

PRIDEiP20901.

Interactioni

Subunit structurei

Homohexamer.

Structurei

Secondary structure

1
436
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 176Combined sources
Beta strandi20 – 278Combined sources
Beta strandi30 – 323Combined sources
Beta strandi35 – 373Combined sources
Helixi41 – 455Combined sources
Beta strandi48 – 503Combined sources
Beta strandi57 – 6812Combined sources
Turni69 – 724Combined sources
Beta strandi73 – 764Combined sources
Helixi81 – 877Combined sources
Helixi90 – 9910Combined sources
Helixi105 – 11612Combined sources
Helixi123 – 1253Combined sources
Helixi126 – 1294Combined sources
Helixi138 – 14912Combined sources
Helixi150 – 1523Combined sources
Helixi165 – 18824Combined sources
Helixi201 – 21010Combined sources
Helixi221 – 23414Combined sources
Helixi241 – 25010Combined sources
Turni251 – 2533Combined sources
Helixi256 – 26813Combined sources
Turni270 – 2745Combined sources
Helixi275 – 28612Combined sources
Helixi289 – 2913Combined sources
Helixi292 – 3009Combined sources
Helixi321 – 33616Combined sources
Helixi343 – 35715Combined sources
Helixi359 – 3635Combined sources
Helixi371 – 38010Combined sources
Helixi385 – 3873Combined sources
Helixi388 – 40922Combined sources
Beta strandi419 – 4224Combined sources
Helixi433 – 4353Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H12X-ray1.85A/B/C/D/E/F1-436[»]
ProteinModelPortaliP20901.
SMRiP20901. Positions 11-436.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20901.

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20901-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASQKEGKL STATISVDGK SAEMPVLSGT LGPDVIDIRK LPAQLGVFTF
60 70 80 90 100
DPGYGETAAC NSKITFIDGD KGVLLHRGYP IAQLDENASY EEVIYLLLNG
110 120 130 140 150
ELPNKVQYDT FTNTLTNHTL LHEQIRNFFN GFRRDAHPMA ILCGTVGALS
160 170 180 190 200
AFYPDANDIA IPANRDLAAM RLIAKIPTIA AWAYKYTQGE AFIYPRNDLN
210 220 230 240 250
YAENFLSMMF ARMSEPYKVN PVLARAMNRI LILHADHEQN ASTSTVRLAG
260 270 280 290 300
STGANPFACI AAGIAALWGP AHGGANEAVL KMLARIGKKE NIPAFIAQVK
310 320 330 340 350
DKNSGVKLMG FGHRVYKNFD PRAKIMQQTC HEVLTELGIK DDPLLDLAVE
360 370 380 390 400
LEKIALSDDY FVQRKLYPNV DFYSGIILKA MGIPTSMFTV LFAVARTTGW
410 420 430
VSQWKEMIEE PGQRISRPRQ LYIGAPQRDY VPLAKR
Length:436
Mass (Da):48,197
Last modified:February 1, 1991 - v1
Checksum:i3E6F0DD5D4216244
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34830 Genomic DNA. Translation: AAA21883.1.
PIRiA35157. YKQPC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34830 Genomic DNA. Translation: AAA21883.1.
PIRiA35157. YKQPC.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H12X-ray1.85A/B/C/D/E/F1-436[»]
ProteinModelPortaliP20901.
SMRiP20901. Positions 11-436.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP20901.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
BioCyciMetaCyc:MONOMER-17983.
BRENDAi2.3.3.16. 33.

Miscellaneous databases

EvolutionaryTraceiP20901.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of genes responsible for acetic acid resistance in Acetobacter aceti."
    Fukaya M., Takemura H., Okumura H., Kawamura Y., Horinouchi S., Beppu T.
    J. Bacteriol. 172:2096-2104(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiCISY_ACEAC
AccessioniPrimary (citable) accession number: P20901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 1, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

3D-structure, Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.