P20852 (CP2A5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2A5
EC=1.14.14.1

Alternative name(s):
CYPIIA5
Coumarin 7-hydroxylase
Cytochrome P450-15-COH
Cytochrome P450-IIA3.2

Gene names

Name:	Cyp2a5
Synonyms:	Cyp2a-5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Exhibits a high coumarin 7-hydroxylase activity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP. Ref.4
Developmental stage	In liver; activity 6 fold higher in females than in males.
Miscellaneous	There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stilbenoid Inferred from expression pattern PubMed 17086191. Source: UniProtKB
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Cytochrome P450 2A5		PRO_0000051667
Sites
	Metal binding	439	1	Iron (heme axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	P20852 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 1C2516D5FA2551D0	FASTA	494	56,741
	10 20 30 40 50 60 MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNFLQLN TEQMYNSLMK 70 80 90 100 110 120 ISQRYGPVFT IYLGPRRIVV LCGQEAVKEA LVDQAEEFSG RGEQATFDWL FKGYGVVFSS 130 140 150 160 170 180 GERAKQLRRF SIATLRDFGV GKRGIEERIQ EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN 190 200 210 220 230 240 VISSIVFGDR FDYEDKEFLS LLRMMLGSFQ FTATSMGQLY EMFSSVMKHL PGPQQQAFKE 250 260 270 280 290 300 LQGLEDFITK KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 310 320 330 340 350 360 AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP YTEAVIHEIQ 370 380 390 400 410 420 RFADMIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK DPKFFSNPKD FNPKHFLDDK 430 440 450 460 470 480 GQFKKNDAFV PFSIGKRYCF GEGLARMELF LFLTNIMQNF HFKSTQAPQD IDVSPRLVGF 490 ATIPPTYTMS FLSR

References

[1]	"The structure and characterization of type I P-450(15) alpha gene as major steroid 15 alpha-hydroxylase and its comparison with type II P-450(15) alpha gene." Lindberg R., Burkhart B., Ichikawa T., Negishi M. J. Biol. Chem. 264:6465-6471(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Kidney.
[2]	"cDNA and amino acid sequence of a new cyp2a isoform overexpressed in chemically induced mouse hepatoma." Jounaidi Y. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 17NC/Z.
[3]	"Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue." Lindberg R., Negishi M. Nature 339:632-634(1989) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
[4]	"Circadian expression of the steroid 15 alpha-hydroxylase (Cyp2a4) and coumarin 7-hydroxylase (Cyp2a5) genes in mouse liver is regulated by the PAR leucine zipper transcription factor DBP." Lavery D.J., Lopez-Molina L., Margueron R., Fleury-Olela F., Conquet F., Schibler U., Bonfils C. Mol. Cell. Biol. 19:6488-6499(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M25211 , M26204, M25205, M25206, M25207, M25208, M25209, M25210 Genomic DNA. Translation: AAA37798.1. X89864 mRNA. Translation: CAA61963.1.
IPI	IPI00123964.
PIR	B33531.
UniGene	Mm.389848.
3D structure databases
ProteinModelPortal	P20852.
SMR	P20852. Positions 31-494.
ModBase	Search...
PTM databases
PhosphoSite	P20852.
Proteomic databases
PaxDb	P20852.
PRIDE	P20852.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:88597. Cyp2a5.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
InParanoid	P20852.
OrthoDB	EOG4BP1BN.
Enzyme and pathway databases
SABIO-RK	P20852.
Gene expression databases
Genevestigator	P20852.
GermOnline	ENSMUSG00000005547. Mus musculus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P20852.
ChEMBL	CHEMBL4085.
SOURCE	Search...

Entry information

Entry name

CP2A5_MOUSE

Accession

Primary (citable) accession number: P20852

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program