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P20852 (CP2A5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2A5

EC=1.14.14.1
Alternative name(s):
CYPIIA5
Coumarin 7-hydroxylase
Cytochrome P450-15-COH
Cytochrome P450-IIA3.2
Gene names
Name:Cyp2a5
Synonyms:Cyp2a-5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Exhibits a high coumarin 7-hydroxylase activity.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP. Ref.4

Developmental stage

In liver; activity 6 fold higher in females than in males.

Miscellaneous

There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Cytochrome P450 2A5
PRO_0000051667

Sites

Metal binding4391Iron (heme axial ligand)

Sequences

Sequence LengthMass (Da)Tools
P20852 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 1C2516D5FA2551D0

FASTA49456,741
        10         20         30         40         50         60 
MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNFLQLN TEQMYNSLMK 

        70         80         90        100        110        120 
ISQRYGPVFT IYLGPRRIVV LCGQEAVKEA LVDQAEEFSG RGEQATFDWL FKGYGVVFSS 

       130        140        150        160        170        180 
GERAKQLRRF SIATLRDFGV GKRGIEERIQ EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN 

       190        200        210        220        230        240 
VISSIVFGDR FDYEDKEFLS LLRMMLGSFQ FTATSMGQLY EMFSSVMKHL PGPQQQAFKE 

       250        260        270        280        290        300 
LQGLEDFITK KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 

       310        320        330        340        350        360 
AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP YTEAVIHEIQ 

       370        380        390        400        410        420 
RFADMIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK DPKFFSNPKD FNPKHFLDDK 

       430        440        450        460        470        480 
GQFKKNDAFV PFSIGKRYCF GEGLARMELF LFLTNIMQNF HFKSTQAPQD IDVSPRLVGF 

       490 
ATIPPTYTMS FLSR 

« Hide

References

[1]"The structure and characterization of type I P-450(15) alpha gene as major steroid 15 alpha-hydroxylase and its comparison with type II P-450(15) alpha gene."
Lindberg R., Burkhart B., Ichikawa T., Negishi M.
J. Biol. Chem. 264:6465-6471(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Kidney.
[2]"cDNA and amino acid sequence of a new cyp2a isoform overexpressed in chemically induced mouse hepatoma."
Jounaidi Y.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 17NC/Z.
[3]"Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue."
Lindberg R., Negishi M.
Nature 339:632-634(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[4]"Circadian expression of the steroid 15 alpha-hydroxylase (Cyp2a4) and coumarin 7-hydroxylase (Cyp2a5) genes in mouse liver is regulated by the PAR leucine zipper transcription factor DBP."
Lavery D.J., Lopez-Molina L., Margueron R., Fleury-Olela F., Conquet F., Schibler U., Bonfils C.
Mol. Cell. Biol. 19:6488-6499(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M25211 expand/collapse EMBL AC list , M26204, M25205, M25206, M25207, M25208, M25209, M25210 Genomic DNA. Translation: AAA37798.1.
X89864 mRNA. Translation: CAA61963.1.
PIRB33531.
UniGeneMm.389848.

3D structure databases

ProteinModelPortalP20852.
SMRP20852. Positions 31-494.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1862789.

Chemistry

BindingDBP20852.
ChEMBLCHEMBL4085.

PTM databases

PhosphoSiteP20852.

Proteomic databases

PaxDbP20852.
PRIDEP20852.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:88597. Cyp2a5.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036992.
HOVERGENHBG015789.
InParanoidP20852.
PhylomeDBP20852.

Enzyme and pathway databases

SABIO-RKP20852.

Gene expression databases

GenevestigatorP20852.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP20852.
SOURCESearch...

Entry information

Entry nameCP2A5_MOUSE
AccessionPrimary (citable) accession number: P20852
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot