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Protein

Cytochrome P450 2A5

Gene

Cyp2a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Exhibits a high coumarin 7-hydroxylase activity.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. response to stilbenoid Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP20852.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A5 (EC:1.14.14.1)
Alternative name(s):
CYPIIA5
Coumarin 7-hydroxylase
Cytochrome P450-15-COH
Cytochrome P450-IIA3.2
Gene namesi
Name:Cyp2a5
Synonyms:Cyp2a-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:88597. Cyp2a5.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Cytochrome P450 2A5PRO_0000051667Add
BLAST

Proteomic databases

MaxQBiP20852.
PaxDbiP20852.
PRIDEiP20852.

PTM databases

PhosphoSiteiP20852.

Expressioni

Tissue specificityi

Liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP.1 Publication

Developmental stagei

In liver; activity 6 fold higher in females than in males.

Gene expression databases

GenevestigatoriP20852.

Interactioni

Protein-protein interaction databases

MINTiMINT-1862789.

Structurei

3D structure databases

ProteinModelPortaliP20852.
SMRiP20852. Positions 31-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP20852.
PhylomeDBiP20852.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNFLQLN
60 70 80 90 100
TEQMYNSLMK ISQRYGPVFT IYLGPRRIVV LCGQEAVKEA LVDQAEEFSG
110 120 130 140 150
RGEQATFDWL FKGYGVVFSS GERAKQLRRF SIATLRDFGV GKRGIEERIQ
160 170 180 190 200
EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN VISSIVFGDR FDYEDKEFLS
210 220 230 240 250
LLRMMLGSFQ FTATSMGQLY EMFSSVMKHL PGPQQQAFKE LQGLEDFITK
260 270 280 290 300
KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF
310 320 330 340 350
AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP
360 370 380 390 400
YTEAVIHEIQ RFADMIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK
410 420 430 440 450
DPKFFSNPKD FNPKHFLDDK GQFKKNDAFV PFSIGKRYCF GEGLARMELF
460 470 480 490
LFLTNIMQNF HFKSTQAPQD IDVSPRLVGF ATIPPTYTMS FLSR
Length:494
Mass (Da):56,741
Last modified:February 1, 1991 - v1
Checksum:i1C2516D5FA2551D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25211
, M26204, M25205, M25206, M25207, M25208, M25209, M25210 Genomic DNA. Translation: AAA37798.1.
X89864 mRNA. Translation: CAA61963.1.
CCDSiCCDS21008.1.
PIRiB33531.
UniGeneiMm.389848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25211
, M26204, M25205, M25206, M25207, M25208, M25209, M25210 Genomic DNA. Translation: AAA37798.1.
X89864 mRNA. Translation: CAA61963.1.
CCDSiCCDS21008.1.
PIRiB33531.
UniGeneiMm.389848.

3D structure databases

ProteinModelPortaliP20852.
SMRiP20852. Positions 31-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1862789.

Chemistry

BindingDBiP20852.
ChEMBLiCHEMBL4085.

PTM databases

PhosphoSiteiP20852.

Proteomic databases

MaxQBiP20852.
PaxDbiP20852.
PRIDEiP20852.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:88597. Cyp2a5.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP20852.
PhylomeDBiP20852.

Enzyme and pathway databases

SABIO-RKP20852.

Miscellaneous databases

PROiP20852.
SOURCEiSearch...

Gene expression databases

GenevestigatoriP20852.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The structure and characterization of type I P-450(15) alpha gene as major steroid 15 alpha-hydroxylase and its comparison with type II P-450(15) alpha gene."
    Lindberg R., Burkhart B., Ichikawa T., Negishi M.
    J. Biol. Chem. 264:6465-6471(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Kidney.
  2. "cDNA and amino acid sequence of a new cyp2a isoform overexpressed in chemically induced mouse hepatoma."
    Jounaidi Y.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 17NC/Z.
  3. "Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue."
    Lindberg R., Negishi M.
    Nature 339:632-634(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  4. "Circadian expression of the steroid 15 alpha-hydroxylase (Cyp2a4) and coumarin 7-hydroxylase (Cyp2a5) genes in mouse liver is regulated by the PAR leucine zipper transcription factor DBP."
    Lavery D.J., Lopez-Molina L., Margueron R., Fleury-Olela F., Conquet F., Schibler U., Bonfils C.
    Mol. Cell. Biol. 19:6488-6499(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCP2A5_MOUSE
AccessioniPrimary (citable) accession number: P20852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 7, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.