Reviewed, UniProtKB/Swiss-Prot P20847 (GUN1_BUTFI)

Names and origin

Protein names

Recommended name:
    Endoglucanase 1
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase 1
    Cellulase 1

Gene names

Name:

end1

Organism

Butyrivibrio fibrisolvens

Taxonomic identifier

831 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Lachnospiraceae › Butyrivibrio

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM2 (carbohydrate binding type-2) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	Endoglucanase 1		PRO_0000184046
Regions
	Domain	448 – 547	100	CBM2
Sites
	Active site	189	1	Proton donor By similarity
	Active site	321	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20847-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: C62EE40E6442ECF9	FASTA	547	61,078
	10 20 30 40 50 60 MHKSKCIKRV FTFLLALFVF VMAIPATKVS AAGGTDRSAT QVVSDMRVGW NIGNSLDSFG 70 80 90 100 110 120 QSYNFPYTSL NETYWGNPAT TKALIDEVAK AGFNTIRIPV SWGQYTTGSD YQIPDFVMNR 130 140 150 160 170 180 VKEVVDYCIV NDMYVILNSH HDINSDYCFY VPNNANKDRS EKYFKSIWTQ IAKEFKNYDY 190 200 210 220 230 240 HLVFETMNEP RLVGHGEEWW FPRNNPSNDI REAVACINDY NQVALDAIRA TGGNNATRCV 250 260 270 280 290 300 MVPGYDASIE GCMTDGFKMP NDTASGRLIL SVHAYIPYYF ALASDTYVTR FDDNLKYDID 310 320 330 340 350 360 SFFNDLNSKF LSRNIPVVVG ETSATNRNNT AERVKWADYY WGRAARYSNV AMVLWDNNIY 370 380 390 400 410 420 QNNSAGSDGE CHMYIDRNSL QWKDPEIIST IMKHVDGTPA TINGKEIPST EQPDPTPVDP 430 440 450 460 470 480 DPTPVDPDPT PVDPDPTPVD PDPQPVDPTP VSGALKAEYT INNWGSGYQV LIKVKNDSAS 490 500 510 520 530 540 RVDGWTLKIS KSEVKIDSSW CVNIAEEGGY YVITPMSWNS SLEPSASVDF GIQGSGSIGT SVNISVQ

References

[1]	"Cloning and sequencing of an endoglucanase (end1) gene from Butyrivibrio fibrisolvens H17c." Berger E., Jones W.A., Jones D.T., Woods D.R. Mol. Gen. Genet. 219:193-198(1989) [PubMed: 2615759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H17C.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17538 Genomic DNA. Translation: CAA35574.1.
PIR	JQ0356.
3D structure databases
HSSP	HSSP built from PDB template 1EDG based on UniProtKB P17901.
ModBase	Search...
Protein family/group databases
CAZy	CBM2. Carbohydrate-Binding Module Family 2. GH5. Glycoside Hydrolase Family 5.
Enzyme and pathway databases
BRENDA	3.2.1.4. 1431.
Family and domain databases
InterPro	IPR001919. CBD_bac. IPR012291. CBD_carb_bd. IPR018366. CBM2_CS. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00553. CBM_2. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view]
SMART	SM00637. CBD_II. 1 hit. [Graphical view]
PROSITE	PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_BUTFI

Accession

Primary (citable) accession number: P20847

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)