P20831 (GYRA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	889 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 889	889	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145254
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Mutagenesis	85	1	S → P: Resistant to fluoroquinolones.
	Sequence conflict	439	1	D → N in CAA50571. Ref.2
	Sequence conflict	483	1	D → E in CAA50571. Ref.2
	Sequence conflict	594	1	E → G in CAA50571. Ref.2
	Sequence conflict	709	1	A → E in AAA73952. Ref.1
	Sequence conflict	815	1	Missing in CAA50571. Ref.2
	Sequence conflict	818	1	T → V in CAA50571. Ref.2
	Sequence conflict	825 – 826	2	Missing AA sequence Ref.2
	Sequence conflict	856	1	D → E in AAA73952. Ref.1
	Sequence conflict	860	1	N → T Ref.1
	Sequence conflict	862	1	E → D Ref.1
	Sequence conflict	884 – 886	3	LDE → S Ref.3
	Sequence conflict	884	1	L → S in CAA50571. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P20831 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 3B826115E06C2250	FASTA	889	99,621
	10 20 30 40 50 60 MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL YGLNEQGMTP 70 80 90 100 110 120 DKSYKKSARI VGDVMGKYHP HGDSSIYEAM VRMAQDFSYR YPLVDGQGNF GSMDGDGAAA 130 140 150 160 170 180 MRYTEARMTK ITLELLRDIN KDTIDFIDNY DGNEREPSVL PARFPNLLAN GASGIAVGMA 190 200 210 220 230 240 TNIPPHNLTE LINGVLSLSK NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS 250 260 270 280 290 300 IQMRSRAVIE ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT 310 320 330 340 350 360 GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE ALVHYLEHQK 370 380 390 400 410 420 TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE SDTDKVAMES LQQRFKLSEK 430 440 450 460 470 480 QAQAILDMRL RRLTGLERDK IEAEYNELLN YISELEAILA DEEVLLQLVR DELTEIRDRF 490 500 510 520 530 540 GDDRRTEIQL GGFEDLEDED LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT 550 560 570 580 590 600 LEEDFVSQLV TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS 610 620 630 640 650 660 TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE LIAVRLTSGQ 670 680 690 700 710 720 EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE VVGLDVAHAN SVDEVLVVTE 730 740 750 760 770 780 NGYGKRTPVN DYRLSNRGGK GIKTATITER NGNVVCITTV TGEEDLMIVT NAGVIIRLDV 790 800 810 820 830 840 ADISQNGRAA QGVRLIRLGD DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV 850 860 870 880 VNDETPGNAI HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSLDEDEE

References

[1]	"Nucleotide sequence of the Staphylococcus aureus gyrB-gyrA locus encoding the DNA gyrase A and B proteins." Margerrison E.E.C., Hopewell R., Fisher L.M. J. Bacteriol. 174:1596-1603(1992) [PubMed: 1311298] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning, sequencing, and expression of the DNA gyrase genes from Staphylococcus aureus." Brockbank S.M.V., Barth P.T. J. Bacteriol. 175:3269-3277(1993) [PubMed: 8388872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-16. Strain: 601055.
[3]	"Quinolone resistance mutations in the DNA gyrase gyrA and gyrB genes of Staphylococcus aureus." Ito H., Yoshida H., Bogaki-Shonai M., Niga T., Hattori H., Nakamura S. Antimicrob. Agents Chemother. 38:2014-2023(1994) [PubMed: 7811012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 12600 / IAM 12544 / NCTC 8532.
[4]	"DNA cloning and organization of the Staphylococcus aureus gyrA and gyrB genes: close homology among gyrase proteins and implications for 4-quinolone action and resistance." Hopewell R., Oram M., Briesewitz R., Fisher L.M. J. Bacteriol. 172:3481-3484(1990) [PubMed: 2160946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-146.
[5]	"DNA gyrase gyrA mutations in ciprofloxacin-resistant strains of Staphylococcus aureus: close similarity with quinolone resistance mutations in Escherichia coli." Sreedharan S., Oram M., Jensen B., Peterson L.R., Fisher L.M. J. Bacteriol. 172:7260-7262(1990) [PubMed: 2174869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-91.
[6]	"Detection of gyrA gene mutations associated with ciprofloxacin resistance in methicillin-resistant Staphylococcus aureus: analysis by polymerase chain reaction and automated direct DNA sequencing." Goswitz J.J., Willard K.E., Fasching C.E., Peterson L.R. Antimicrob. Agents Chemother. 36:1166-1169(1992) [PubMed: 1510411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86227 Genomic DNA. Translation: AAA73952.1. X71437 Genomic DNA. Translation: CAA50571.1. D10489 Genomic DNA. Translation: BAA01370.1. M37915 Genomic DNA. Translation: AAA26636.1.
PIR	B40585.
3D structure databases
ProteinModelPortal	P20831.
SMR	P20831. Positions 31-489.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_STAAU

Accession

Primary (citable) accession number: P20831

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program