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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei123 – 1231O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotation
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi85 – 851S → P: Resistant to fluoroquinolones.

Chemistry

ChEMBLiCHEMBL3038482.
DrugBankiDB00537. Ciprofloxacin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 889889DNA gyrase subunit APRO_0000145254Add
BLAST

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Protein-protein interaction databases

STRINGi93062.SACOL0006.

Chemistry

BindingDBiP20831.

Structurei

Secondary structure

1
889
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133Combined sources
Helixi14 – 3219Combined sources
Turni38 – 403Combined sources
Helixi44 – 5512Combined sources
Helixi67 – 7711Combined sources
Helixi83 – 9412Combined sources
Turni96 – 983Combined sources
Beta strandi103 – 1086Combined sources
Turni121 – 1233Combined sources
Beta strandi125 – 1284Combined sources
Helixi130 – 1356Combined sources
Turni137 – 1426Combined sources
Beta strandi146 – 1483Combined sources
Beta strandi152 – 1598Combined sources
Helixi166 – 1705Combined sources
Beta strandi172 – 1754Combined sources
Beta strandi180 – 1834Combined sources
Helixi188 – 20013Combined sources
Helixi206 – 2094Combined sources
Turni210 – 2123Combined sources
Beta strandi223 – 2253Combined sources
Helixi228 – 2369Combined sources
Beta strandi237 – 2448Combined sources
Beta strandi246 – 2538Combined sources
Beta strandi256 – 2638Combined sources
Helixi270 – 28213Combined sources
Beta strandi286 – 2949Combined sources
Turni298 – 3003Combined sources
Beta strandi304 – 3085Combined sources
Helixi314 – 32411Combined sources
Beta strandi328 – 3347Combined sources
Beta strandi336 – 3394Combined sources
Beta strandi342 – 3454Combined sources
Helixi348 – 38942Combined sources
Helixi391 – 3999Combined sources
Helixi404 – 41512Combined sources
Helixi419 – 4279Combined sources
Helixi430 – 4334Combined sources
Helixi435 – 4373Combined sources
Helixi438 – 46023Combined sources
Helixi462 – 48019Combined sources
Beta strandi486 – 4894Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4PLBX-ray2.69B/D2-491[»]
5BS3X-ray2.65B/D2-491[»]
ProteinModelPortaliP20831.
SMRiP20831. Positions 31-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi528 – 5347GyrA-boxUniRule annotation

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.

Sequencei

Sequence statusi: Complete.

P20831-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL
60 70 80 90 100
YGLNEQGMTP DKSYKKSARI VGDVMGKYHP HGDSSIYEAM VRMAQDFSYR
110 120 130 140 150
YPLVDGQGNF GSMDGDGAAA MRYTEARMTK ITLELLRDIN KDTIDFIDNY
160 170 180 190 200
DGNEREPSVL PARFPNLLAN GASGIAVGMA TNIPPHNLTE LINGVLSLSK
210 220 230 240 250
NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS IQMRSRAVIE
260 270 280 290 300
ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT
310 320 330 340 350
GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE
360 370 380 390 400
ALVHYLEHQK TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE
410 420 430 440 450
SDTDKVAMES LQQRFKLSEK QAQAILDMRL RRLTGLERDK IEAEYNELLN
460 470 480 490 500
YISELEAILA DEEVLLQLVR DELTEIRDRF GDDRRTEIQL GGFEDLEDED
510 520 530 540 550
LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT LEEDFVSQLV
560 570 580 590 600
TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS
610 620 630 640 650
TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE
660 670 680 690 700
LIAVRLTSGQ EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE
710 720 730 740 750
VVGLDVAHAN SVDEVLVVTE NGYGKRTPVN DYRLSNRGGK GIKTATITER
760 770 780 790 800
NGNVVCITTV TGEEDLMIVT NAGVIIRLDV ADISQNGRAA QGVRLIRLGD
810 820 830 840 850
DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV VNDETPGNAI
860 870 880
HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSLDEDEE
Length:889
Mass (Da):99,621
Last modified:February 1, 1996 - v3
Checksum:i3B826115E06C2250
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti439 – 4391D → N in CAA50571 (PubMed:8388872).Curated
Sequence conflicti483 – 4831D → E in CAA50571 (PubMed:8388872).Curated
Sequence conflicti594 – 5941E → G in CAA50571 (PubMed:8388872).Curated
Sequence conflicti709 – 7091A → E in AAA73952 (PubMed:1311298).Curated
Sequence conflicti815 – 8151Missing in CAA50571 (PubMed:8388872).Curated
Sequence conflicti818 – 8181T → V in CAA50571 (PubMed:8388872).Curated
Sequence conflicti825 – 8262Missing AA sequence (PubMed:8388872).Curated
Sequence conflicti856 – 8561D → E in AAA73952 (PubMed:1311298).Curated
Sequence conflicti860 – 8601N → T in AAA73952 (PubMed:1311298).Curated
Sequence conflicti862 – 8621E → D in AAA73952 (PubMed:1311298).Curated
Sequence conflicti884 – 8863LDE → S in BAA01370 (PubMed:7811012).Curated
Sequence conflicti884 – 8841L → S in CAA50571 (PubMed:8388872).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86227 Genomic DNA. Translation: AAA73952.1.
X71437 Genomic DNA. Translation: CAA50571.1.
D10489 Genomic DNA. Translation: BAA01370.1.
M37915 Genomic DNA. Translation: AAA26636.1.
PIRiB40585.
RefSeqiWP_000819084.1. NZ_LNOO01000002.1.
WP_000819086.1. NZ_LJBL01000013.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86227 Genomic DNA. Translation: AAA73952.1.
X71437 Genomic DNA. Translation: CAA50571.1.
D10489 Genomic DNA. Translation: BAA01370.1.
M37915 Genomic DNA. Translation: AAA26636.1.
PIRiB40585.
RefSeqiWP_000819084.1. NZ_LNOO01000002.1.
WP_000819086.1. NZ_LJBL01000013.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4PLBX-ray2.69B/D2-491[»]
5BS3X-ray2.65B/D2-491[»]
ProteinModelPortaliP20831.
SMRiP20831. Positions 31-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL0006.

Chemistry

BindingDBiP20831.
ChEMBLiCHEMBL3038482.
DrugBankiDB00537. Ciprofloxacin.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGYRA_STAAU
AccessioniPrimary (citable) accession number: P20831
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.