P20825 (POL2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retrovirus-related Pol polyprotein from transposon 297

Including the following 3 domains:

1. Protease
   EC=3.4.23.-
2. Reverse transcriptase
   EC=2.7.7.49
3. Endonuclease

Gene names

Name:

pol

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1059 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Sequence similarities	Contains 1 integrase catalytic domain. Contains 1 peptidase A2 domain. Contains 1 reverse transcriptase domain.

Ontologies

Keywords
Molecular function	Aspartyl protease Endonuclease Hydrolase Nuclease Nucleotidyltransferase Protease RNA-directed DNA polymerase Transferase
Technical term	Transposable element
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: InterPro RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1059	1059	Retrovirus-related Pol polyprotein from transposon 297		PRO_0000199556
Regions
	Domain	230 – 414	185	Reverse transcriptase
	Domain	812 – 967	156	Integrase catalytic
Sites
	Active site	30	1	For protease activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20825 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 3905CF38E914173D	FASTA	1,059	123,311
	10 20 30 40 50 60 TKRKFSVNSS GKYEYIKIVY KGRSYKCLLD TGSTINMINE NIFCLPIQNS RCEVLTSNGP 70 80 90 100 110 120 ITLNDLIMLP RNSIFKKTEP FYVHRFSNNY DMLIGRKLLK NAQSVINYKN DTVTLFDQTY 130 140 150 160 170 180 KLITSESERN QNLYIQRTPE SIASSDQESI KKLDFSQFRL DHLNQEETFK LKGLLNKFRN 190 200 210 220 230 240 LEYKEGEKLT FTNTIKHVLN TTHNSPIYSK QYPLAQTHEI EVENQVQEML NQGLIRESNS 250 260 270 280 290 300 PYNSPTWVVP KKPDASGANK YRVVIDYRKL NEITIPDRYP IPNMDEILGK LGKCQYFTTI 310 320 330 340 350 360 DLAKGFHQIE MDEESISKTA FSTKSGHYEY LRMPFGLRNA PATFQRCMNN ILRPLLNKHC 370 380 390 400 410 420 LVYLDDIIIF STSLTEHLNS IQLVFTKLAD ANLKLQLDKC EFLKKEANFL GHIVTPDGIK 430 440 450 460 470 480 PNPIKVKAIV SYPIPTKDKE IRAFLGLTGY YRKFIPNYAD IAKPMTSCLK KRTKIDTQKL 490 500 510 520 530 540 EYIEAFEKLK ALIIRDPILQ LPDFEKKFVL TTDASNLALG AVLSQNGHPI SFISRTLNDH 550 560 570 580 590 600 ELNYSAIEKE LLAIVWATKT FRHYLLGRQF LIASDHQPLR WLHNLKEPGA KLERWRVRLS 610 620 630 640 650 660 EYQFKIDYIK GKENSVADAL SRIKIEENHH SEATQHSAEE DNSNLIHLTE KPINYFKKQI 670 680 690 700 710 720 IFIKSDKNKV EHSKIFGNSI TTIQYDVMTL EKAKQILLDH FIHRNITIYI ESDVDFEIVQ 730 740 750 760 770 780 RAHIEIVNTT YTKVIRSLFL LKNVGSYAEF KEIILQSHEK LLHPGIQKMT KLFKENHFFP 790 800 810 820 830 840 NSQLLIQNII NECNICNLAK TEHRNTKMPL KITPNPEHCR EKFVVDIYSS EGKHYISCID 850 860 870 880 890 900 IYSKFATLEQ IKTKDWIECR NALMRIFNQL GKPKLLKADR DGAFSSLALK RWLEEEEVEL 910 920 930 940 950 960 QLNTAKNGVA DVERLHKTIN EKIRIINSSD DEEVKLSKIE TILYTYNQKI KHDTTGQRPA 970 980 990 1000 1010 1020 QIFLYAGHPI LDTQKIKEKK IEKINEDRRE FNIDTNYRKG PLQKGKLENP FKPTKNVEQT 1030 1040 1050 DPDHYKITNR NRVTHYYKTQ FKKQKKNNKL SISQAPGTR

References

[1]	"Complete nucleotide sequence and genome organization of a Drosophila transposable genetic element, 297." Inouye S., Yuki S., Saigo K. Eur. J. Biochem. 154:417-425(1986) [PubMed: 2417839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03431 Genomic DNA. Translation: CAB57796.1. Sequence problems.
PIR	B24872.
3D structure databases
ProteinModelPortal	P20825.
ModBase	Search...
Protein family/group databases
MEROPS	A02.052.
Proteomic databases
PRIDE	P20825.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
FlyBase	FBgn0027622. 297\pol.
Family and domain databases
InterPro	IPR001584. Integrase_cat-core. IPR018061. Pept_A2A_retrovirus_sg. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR012337. RNaseH-like_dom. IPR000477. RVT. [Graphical view]
Pfam	PF00665. rve. 1 hit. PF00077. RVP. 1 hit. PF00078. RVT_1. 1 hit. [Graphical view]
SUPFAM	SSF50630. Pept_Aspartic. 1 hit. SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 1 hit. PS50994. INTEGRASE. 1 hit. PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL2_DROME

Accession

Primary (citable) accession number: P20825

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project