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Protein

Cytochrome P450 4A14

Gene

Cyp4a14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

A long-chain fatty acid + NADPH + O2 = an omega-hydroxy-long-chain fatty acid + NADP+ + H2O.By similarity

Cofactori

heme2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei318Heme (covalent; via 1 link)2 Publications1
Metal bindingi454Iron (heme axial ligand)2 Publications1

GO - Molecular functioni

  • alkane 1-monooxygenase activity Source: RGD
  • arachidonic acid monooxygenase activity Source: RGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • arachidonic acid metabolic process Source: RGD
  • icosanoid biosynthetic process Source: RGD
  • kidney development Source: RGD
  • lauric acid metabolic process Source: RGD
  • linoleic acid metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Chemistry databases

SwissLipidsiSLP:000000744.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A14
Alternative name(s):
CYPIVA14
Cytochrome P450-LA-omega 3
Lauric acid omega-hydroxylase
Long-chain fatty acid omega-monooxygenase (EC:1.14.13.205)
Gene namesi
Name:Cyp4a14
Synonyms:Cyp4a-3, Cyp4a3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631356. Cyp4a3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi318E → A: Loss of covalent heme binding. 1 Publication1
Mutagenesisi318E → D: Significant reduction in covalent heme binding. 1 Publication1
Mutagenesisi318E → Q: Significant reduction in covalent heme binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00000035692 – 4Removed in mature form1 Publication3
ChainiPRO_00000035705 – 507Cytochrome P450 4A14Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei437PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20817.
PRIDEiP20817.

PTM databases

iPTMnetiP20817.
PhosphoSitePlusiP20817.

Expressioni

Inductioni

By clofibrate.

Interactioni

Protein-protein interaction databases

MINTiMINT-4571238.
STRINGi10116.ENSRNOP00000042072.

Structurei

3D structure databases

ProteinModelPortaliP20817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP20817.
KOiK07425.
PhylomeDBiP20817.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFSVFTPTR SLDGVSGFFQ GAFLLSLFLV LFKAVQFYLR RQWLLKALEK
60 70 80 90 100
FPSTPSHWLW GHDLKDREFQ QVLTWVEKFP GACLQWLSGS KTRVLLYDPD
110 120 130 140 150
YVKVVLGRSD PKASGIYQFL APWIGYGLLL LNGKKWFQHR RMLTPAFHYG
160 170 180 190 200
ILKPYVKIMA DSVNIMLDKW EKLDDQDHPL EIFHYVSLMT LDTVMKCAFS
210 220 230 240 250
HQGSVQLDVN SRSYTKAVED LNNLTFFRVR SAFYGNSIIY NMSSDGRLSR
260 270 280 290 300
RACQIAHEHT DGVIKMRKAQ LQNEEELQKA RKKRHLDFLD ILLFAKMEDG
310 320 330 340 350
KSLSDEDLRA EVDTFMFEGH DTTASGISWV FYALATHPEH QERCREEVQS
360 370 380 390 400
ILGDGTSVTW DHLDQIPYTT MCIKEALRLY PPVPSVSREL SSPVTFPDGR
410 420 430 440 450
SIPKGITTTI LIYGLHHNPS YWPNPKVFDP SRFSPDSPRH SHAYLPFSGG
460 470 480 490 500
ARNCIGKQFA MNELKVAVAL TLLRFELLPD PTRIPVPMAR LVLKSKNGIH

LRLKKLR
Length:507
Mass (Da):58,232
Last modified:February 1, 1996 - v2
Checksum:i1A972C6F967BF1AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33936 mRNA. Translation: AAA41458.1.
PIRiA32966.
PC4351.
RefSeqiNP_786936.1. NM_175760.2.
UniGeneiRn.33492.

Genome annotation databases

GeneIDi298423.
KEGGirno:298423.
UCSCiRGD:631356. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33936 mRNA. Translation: AAA41458.1.
PIRiA32966.
PC4351.
RefSeqiNP_786936.1. NM_175760.2.
UniGeneiRn.33492.

3D structure databases

ProteinModelPortaliP20817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4571238.
STRINGi10116.ENSRNOP00000042072.

Chemistry databases

SwissLipidsiSLP:000000744.

PTM databases

iPTMnetiP20817.
PhosphoSitePlusiP20817.

Proteomic databases

PaxDbiP20817.
PRIDEiP20817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi298423.
KEGGirno:298423.
UCSCiRGD:631356. rat.

Organism-specific databases

CTDi298423.
RGDi631356. Cyp4a3.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP20817.
KOiK07425.
PhylomeDBiP20817.

Miscellaneous databases

PROiP20817.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP4AE_RAT
AccessioniPrimary (citable) accession number: P20817
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.