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Protein

Cytochrome P450 4A14

Gene

Cyp4a14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

A long-chain fatty acid + NADPH + O2 = an omega-hydroxy-long-chain fatty acid + NADP+ + H2O.By similarity

Cofactori

heme2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei318 – 3181Heme (covalent; via 1 link)2 Publications
Metal bindingi454 – 4541Iron (heme axial ligand)2 Publications

GO - Molecular functioni

  • alkane 1-monooxygenase activity Source: RGD
  • arachidonic acid monooxygenase activity Source: RGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro

GO - Biological processi

  • arachidonic acid metabolic process Source: RGD
  • icosanoid biosynthetic process Source: RGD
  • kidney development Source: RGD
  • lauric acid metabolic process Source: RGD
  • linoleic acid metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Chemistry

SwissLipidsiSLP:000000744.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A14
Alternative name(s):
CYPIVA14
Cytochrome P450-LA-omega 3
Lauric acid omega-hydroxylase
Long-chain fatty acid omega-monooxygenase (EC:1.14.13.205)
Gene namesi
Name:Cyp4a14
Synonyms:Cyp4a-3, Cyp4a3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631356. Cyp4a3.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi318 – 3181E → A: Loss of covalent heme binding. 1 Publication
Mutagenesisi318 – 3181E → D: Significant reduction in covalent heme binding. 1 Publication
Mutagenesisi318 – 3181E → Q: Significant reduction in covalent heme binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 44Removed in mature form1 PublicationPRO_0000003569
Chaini5 – 507503Cytochrome P450 4A14PRO_0000003570Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei437 – 4371PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20817.
PRIDEiP20817.

PTM databases

iPTMnetiP20817.
PhosphoSiteiP20817.

Expressioni

Inductioni

By clofibrate.

Interactioni

Protein-protein interaction databases

MINTiMINT-4571238.
STRINGi10116.ENSRNOP00000042072.

Structurei

3D structure databases

ProteinModelPortaliP20817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP20817.
KOiK07425.
PhylomeDBiP20817.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFSVFTPTR SLDGVSGFFQ GAFLLSLFLV LFKAVQFYLR RQWLLKALEK
60 70 80 90 100
FPSTPSHWLW GHDLKDREFQ QVLTWVEKFP GACLQWLSGS KTRVLLYDPD
110 120 130 140 150
YVKVVLGRSD PKASGIYQFL APWIGYGLLL LNGKKWFQHR RMLTPAFHYG
160 170 180 190 200
ILKPYVKIMA DSVNIMLDKW EKLDDQDHPL EIFHYVSLMT LDTVMKCAFS
210 220 230 240 250
HQGSVQLDVN SRSYTKAVED LNNLTFFRVR SAFYGNSIIY NMSSDGRLSR
260 270 280 290 300
RACQIAHEHT DGVIKMRKAQ LQNEEELQKA RKKRHLDFLD ILLFAKMEDG
310 320 330 340 350
KSLSDEDLRA EVDTFMFEGH DTTASGISWV FYALATHPEH QERCREEVQS
360 370 380 390 400
ILGDGTSVTW DHLDQIPYTT MCIKEALRLY PPVPSVSREL SSPVTFPDGR
410 420 430 440 450
SIPKGITTTI LIYGLHHNPS YWPNPKVFDP SRFSPDSPRH SHAYLPFSGG
460 470 480 490 500
ARNCIGKQFA MNELKVAVAL TLLRFELLPD PTRIPVPMAR LVLKSKNGIH

LRLKKLR
Length:507
Mass (Da):58,232
Last modified:February 1, 1996 - v2
Checksum:i1A972C6F967BF1AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33936 mRNA. Translation: AAA41458.1.
PIRiA32966.
PC4351.
RefSeqiNP_786936.1. NM_175760.2.
UniGeneiRn.33492.

Genome annotation databases

GeneIDi298423.
KEGGirno:298423.
UCSCiRGD:631356. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33936 mRNA. Translation: AAA41458.1.
PIRiA32966.
PC4351.
RefSeqiNP_786936.1. NM_175760.2.
UniGeneiRn.33492.

3D structure databases

ProteinModelPortaliP20817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4571238.
STRINGi10116.ENSRNOP00000042072.

Chemistry

SwissLipidsiSLP:000000744.

PTM databases

iPTMnetiP20817.
PhosphoSiteiP20817.

Proteomic databases

PaxDbiP20817.
PRIDEiP20817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi298423.
KEGGirno:298423.
UCSCiRGD:631356. rat.

Organism-specific databases

CTDi298423.
RGDi631356. Cyp4a3.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP20817.
KOiK07425.
PhylomeDBiP20817.

Miscellaneous databases

NextBioi643645.
PROiP20817.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The rat clofibrate-inducible CYP4A subfamily. II. cDNA sequence of IVA3, mapping of the Cyp4a locus to mouse chromosome 4, and coordinate and tissue-specific regulation of the CYP4A genes."
    Kimura S., Hardwick J.P., Kozak C.A., Gonzalez F.J.
    DNA 8:517-525(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Induction of renal cytochrome P-450 in hepatic microsomes of diabetic rats."
    Imaoka S., Shimojo N., Funae Y.
    Biochem. Biophys. Res. Commun. 152:680-687(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 5-19.
  3. "Covalently linked heme in cytochrome P4504A fatty acid hydroxylases."
    Hoch U., Ortiz de Montellano P.R.
    J. Biol. Chem. 276:11339-11346(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT, MUTAGENESIS.
  4. "Autocatalytic mechanism and consequences of covalent heme attachment in the cytochrome P4504A family."
    LeBrun L.A., Hoch U., Ortiz de Montellano P.R.
    J. Biol. Chem. 277:12755-12761(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT, MUTAGENESIS OF GLU-318.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-437, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCP4AE_RAT
AccessioniPrimary (citable) accession number: P20817
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: February 17, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.